Produção de ficocianina por Aphanothece microscopica Nägeli a partir de vinhaça de cana-de-açúcar
Autor(a) principal: | |
---|---|
Data de Publicação: | 2018 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFSCAR |
Texto Completo: | https://repositorio.ufscar.br/handle/ufscar/10249 |
Resumo: | Microalgae cultivation has a great diversity of applications. Pigments produced by microalgae are largely used in many industries, such as food, pharmaceutical and cosmetics. Phycobiliproteins are photosynthetic pigments that capture around 50% of the light needed to maintain the metabolism of cyanobacteria. A big advantage for the use of microorganisms to produce pigments is that they can assimilate low-cost energy sources, including wastewaters. In this context, the present work proposed to evaluate the production of phycocyanin by Aphanothece microscopica Nägeli, through its cultivation in vinasse, an effluent generated abundantly in Brazil. The mixotrophic cultivation of A. microscopica in BG11 medium supplemented with vinasse showed superior performance than the photoautotrophic growth, in terms of phycocyanin accumulation, with specific rate of production (μP) and maximum productivity (Prodmax) of 9.86 mg phycocyanin mg biomass/h and 0.0047 mg phycocyanin/L/h, respectively. For the heterotrophic cultivation, the highest phycocyanin production occurred after 6 hours, with μP and Prodmax of 22.59 mg phycocyanin/mg biomass/h and 0.0067 mg phycocyanin/L/h, respectively. No statistically significant difference was found for wide ranges of pH, inoculum and salinity tested. The conditions which had the highest and lowest performance relative to μPmax were selected for further experiments on a benchtop bioreactor. The condition with the highest μPmax showed maximum productivity of 49.18 mg phycocyanin/mg biomass/h, and the lowest, 33.83 mg phycocyanin/mg biomass. Phycocyanin extraction with hydrochloric acid 12 mol/L was the most efficient method, yielding up to 5,6 times more phycocyanin than sonication with glass pearls and phosphate buffer extractions . A. microscopica biomass contained 4.6% of proteins and 6.38% of lipids. Those results show that vinasse can be used as a supplement for the production of phycocyanin by A. microscopica. |
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Morais, Dayane VanessaBastos, Reinaldo Gasparhttp://lattes.cnpq.br/9329363278429665http://lattes.cnpq.br/511270583286527652687f24-71e8-477b-9db5-456f13cd0abf2018-07-03T14:43:12Z2018-07-03T14:43:12Z2018-02-26MORAIS, Dayane Vanessa. Produção de ficocianina por Aphanothece microscopica Nägeli a partir de vinhaça de cana-de-açúcar. 2018. Dissertação (Mestrado em Produção Vegetal e Bioprocessos Associados) – Universidade Federal de São Carlos, Araras, 2018. Disponível em: https://repositorio.ufscar.br/handle/ufscar/10249.https://repositorio.ufscar.br/handle/ufscar/10249Microalgae cultivation has a great diversity of applications. Pigments produced by microalgae are largely used in many industries, such as food, pharmaceutical and cosmetics. Phycobiliproteins are photosynthetic pigments that capture around 50% of the light needed to maintain the metabolism of cyanobacteria. A big advantage for the use of microorganisms to produce pigments is that they can assimilate low-cost energy sources, including wastewaters. In this context, the present work proposed to evaluate the production of phycocyanin by Aphanothece microscopica Nägeli, through its cultivation in vinasse, an effluent generated abundantly in Brazil. The mixotrophic cultivation of A. microscopica in BG11 medium supplemented with vinasse showed superior performance than the photoautotrophic growth, in terms of phycocyanin accumulation, with specific rate of production (μP) and maximum productivity (Prodmax) of 9.86 mg phycocyanin mg biomass/h and 0.0047 mg phycocyanin/L/h, respectively. For the heterotrophic cultivation, the highest phycocyanin production occurred after 6 hours, with μP and Prodmax of 22.59 mg phycocyanin/mg biomass/h and 0.0067 mg phycocyanin/L/h, respectively. No statistically significant difference was found for wide ranges of pH, inoculum and salinity tested. The conditions which had the highest and lowest performance relative to μPmax were selected for further experiments on a benchtop bioreactor. The condition with the highest μPmax showed maximum productivity of 49.18 mg phycocyanin/mg biomass/h, and the lowest, 33.83 mg phycocyanin/mg biomass. Phycocyanin extraction with hydrochloric acid 12 mol/L was the most efficient method, yielding up to 5,6 times more phycocyanin than sonication with glass pearls and phosphate buffer extractions . A. microscopica biomass contained 4.6% of proteins and 6.38% of lipids. Those results show that vinasse can be used as a supplement for the production of phycocyanin by A. microscopica.O cultivo de microalgas apresenta grande diversidade de aplicações. Os pigmentos extraídos de microalgas podem ser amplamente utilizados em várias indústrias, incluindo a alimentícia, farmacêutica e cosmética. As ficobiliproteínas são pigmentos fotossintéticos e capturam cerca de 50% da luz necessária para manutenção do metabolismo das cianobactérias. Uma grande vantagem da utilização de micro-organismos é que estes podem assimilar fontes de energia de baixo custo, incluindo águas residuárias. Neste contexto, o presente trabalho propôs a avaliação da produção de ficocianina por Aphanothece microscopica Nägeli a partir de vinhaça, um efluente produzido em larga escala no Brasil. O cultivo mixotrófico de A. microscopica em meio BG11 suplementado com vinhaça apresentou desempenho superior ao cultivo fotoautotrófico em termos de acúmulo de ficocianina, com velocidade específica de produção (μP) e produtividade máxima (Prodmáx) de 9,86 mg ficocianina/mg biomassa/h e 0,0047 mg ficocianina/L/h, respectivamente. Para o cultivo heterotrófico, a maior produção de ficocianina ocorreu após 6 horas, com μP e Prodmáx de 22,59 mg ficocianina/mg biomassa/h e 0,0067 mg ficocianina/L/h, respectivamente. Não foram encontradas diferenças significativas na produção de ficocianina para amplas faixas de pH, inóculo e salinidade testadas; as condições que obtiveram o melhor e pior desempenho em termos de μPmáx foram selecionadas para se avaliar a produção do pigmento em processo escalonado. A condição de melhor desempenho apresentou μPmáx de 49,18 mg ficocianina/mg biomassa/h, e a pior, 33,83 mg ficocianina/mg biomassa/h. A extração de ficocianina com ácido clorídrico 12 mol/L mostrou-se até 5,6 vezes mais eficiente do que a sonicação com pérolas de vidro e tampão fosfato. A biomassa de A. microscopica apresentou 4,6% de proteínas e 6,38% de lipídios. Os resultados indicam que a vinhaça pode ser utilizada como suplemento para a produção de ficocianina por A. microscopica.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)porUniversidade Federal de São CarlosCâmpus ArarasPrograma de Pós-Graduação em Produção Vegetal e Bioprocessos Associados - PPGPVBA-ArUFSCarCianobactériasFicobiliproteínasMicroalgasPigmentosCultivo heterotróficoCyanobacteriaPhycobiliproteinsMicroalgaePigmentsHeterotrophic CultivationCIENCIAS AGRARIASProdução de ficocianina por Aphanothece microscopica Nägeli a partir de vinhaça de cana-de-açúcarPhycocyanin production by Aphanothece microscopica Nägeli from sugarcane vinasseinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisOnline600cb09db38-7017-4f6a-a8e0-d6de60933623info:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFSCARinstname:Universidade Federal de São Carlos (UFSCAR)instacron:UFSCARORIGINALMORAIS_Dayane_2018.pdfMORAIS_Dayane_2018.pdfapplication/pdf1985020https://repositorio.ufscar.br/bitstream/ufscar/10249/6/MORAIS_Dayane_2018.pdfb9a1486a583db360fcd413f1e122f7c0MD56LICENSElicense.txtlicense.txttext/plain; charset=utf-81957https://repositorio.ufscar.br/bitstream/ufscar/10249/5/license.txtae0398b6f8b235e40ad82cba6c50031dMD55TEXTMORAIS_Dayane_2018.pdf.txtMORAIS_Dayane_2018.pdf.txtExtracted texttext/plain184470https://repositorio.ufscar.br/bitstream/ufscar/10249/7/MORAIS_Dayane_2018.pdf.txt216a007a88f64a074799309f2b2e2a9fMD57THUMBNAILMORAIS_Dayane_2018.pdf.jpgMORAIS_Dayane_2018.pdf.jpgIM Thumbnailimage/jpeg6972https://repositorio.ufscar.br/bitstream/ufscar/10249/8/MORAIS_Dayane_2018.pdf.jpgdc1a782d41a8ad3464568ca9fb105eccMD58ufscar/102492023-09-18 18:31:43.1oai:repositorio.ufscar.br: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Repositório InstitucionalPUBhttps://repositorio.ufscar.br/oai/requestopendoar:43222023-09-18T18:31:43Repositório Institucional da UFSCAR - Universidade Federal de São Carlos (UFSCAR)false |
dc.title.por.fl_str_mv |
Produção de ficocianina por Aphanothece microscopica Nägeli a partir de vinhaça de cana-de-açúcar |
dc.title.alternative.eng.fl_str_mv |
Phycocyanin production by Aphanothece microscopica Nägeli from sugarcane vinasse |
title |
Produção de ficocianina por Aphanothece microscopica Nägeli a partir de vinhaça de cana-de-açúcar |
spellingShingle |
Produção de ficocianina por Aphanothece microscopica Nägeli a partir de vinhaça de cana-de-açúcar Morais, Dayane Vanessa Cianobactérias Ficobiliproteínas Microalgas Pigmentos Cultivo heterotrófico Cyanobacteria Phycobiliproteins Microalgae Pigments Heterotrophic Cultivation CIENCIAS AGRARIAS |
title_short |
Produção de ficocianina por Aphanothece microscopica Nägeli a partir de vinhaça de cana-de-açúcar |
title_full |
Produção de ficocianina por Aphanothece microscopica Nägeli a partir de vinhaça de cana-de-açúcar |
title_fullStr |
Produção de ficocianina por Aphanothece microscopica Nägeli a partir de vinhaça de cana-de-açúcar |
title_full_unstemmed |
Produção de ficocianina por Aphanothece microscopica Nägeli a partir de vinhaça de cana-de-açúcar |
title_sort |
Produção de ficocianina por Aphanothece microscopica Nägeli a partir de vinhaça de cana-de-açúcar |
author |
Morais, Dayane Vanessa |
author_facet |
Morais, Dayane Vanessa |
author_role |
author |
dc.contributor.authorlattes.por.fl_str_mv |
http://lattes.cnpq.br/5112705832865276 |
dc.contributor.author.fl_str_mv |
Morais, Dayane Vanessa |
dc.contributor.advisor1.fl_str_mv |
Bastos, Reinaldo Gaspar |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/9329363278429665 |
dc.contributor.authorID.fl_str_mv |
52687f24-71e8-477b-9db5-456f13cd0abf |
contributor_str_mv |
Bastos, Reinaldo Gaspar |
dc.subject.por.fl_str_mv |
Cianobactérias Ficobiliproteínas Microalgas Pigmentos Cultivo heterotrófico |
topic |
Cianobactérias Ficobiliproteínas Microalgas Pigmentos Cultivo heterotrófico Cyanobacteria Phycobiliproteins Microalgae Pigments Heterotrophic Cultivation CIENCIAS AGRARIAS |
dc.subject.eng.fl_str_mv |
Cyanobacteria Phycobiliproteins Microalgae Pigments Heterotrophic Cultivation |
dc.subject.cnpq.fl_str_mv |
CIENCIAS AGRARIAS |
description |
Microalgae cultivation has a great diversity of applications. Pigments produced by microalgae are largely used in many industries, such as food, pharmaceutical and cosmetics. Phycobiliproteins are photosynthetic pigments that capture around 50% of the light needed to maintain the metabolism of cyanobacteria. A big advantage for the use of microorganisms to produce pigments is that they can assimilate low-cost energy sources, including wastewaters. In this context, the present work proposed to evaluate the production of phycocyanin by Aphanothece microscopica Nägeli, through its cultivation in vinasse, an effluent generated abundantly in Brazil. The mixotrophic cultivation of A. microscopica in BG11 medium supplemented with vinasse showed superior performance than the photoautotrophic growth, in terms of phycocyanin accumulation, with specific rate of production (μP) and maximum productivity (Prodmax) of 9.86 mg phycocyanin mg biomass/h and 0.0047 mg phycocyanin/L/h, respectively. For the heterotrophic cultivation, the highest phycocyanin production occurred after 6 hours, with μP and Prodmax of 22.59 mg phycocyanin/mg biomass/h and 0.0067 mg phycocyanin/L/h, respectively. No statistically significant difference was found for wide ranges of pH, inoculum and salinity tested. The conditions which had the highest and lowest performance relative to μPmax were selected for further experiments on a benchtop bioreactor. The condition with the highest μPmax showed maximum productivity of 49.18 mg phycocyanin/mg biomass/h, and the lowest, 33.83 mg phycocyanin/mg biomass. Phycocyanin extraction with hydrochloric acid 12 mol/L was the most efficient method, yielding up to 5,6 times more phycocyanin than sonication with glass pearls and phosphate buffer extractions . A. microscopica biomass contained 4.6% of proteins and 6.38% of lipids. Those results show that vinasse can be used as a supplement for the production of phycocyanin by A. microscopica. |
publishDate |
2018 |
dc.date.accessioned.fl_str_mv |
2018-07-03T14:43:12Z |
dc.date.available.fl_str_mv |
2018-07-03T14:43:12Z |
dc.date.issued.fl_str_mv |
2018-02-26 |
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info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
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masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
MORAIS, Dayane Vanessa. Produção de ficocianina por Aphanothece microscopica Nägeli a partir de vinhaça de cana-de-açúcar. 2018. Dissertação (Mestrado em Produção Vegetal e Bioprocessos Associados) – Universidade Federal de São Carlos, Araras, 2018. Disponível em: https://repositorio.ufscar.br/handle/ufscar/10249. |
dc.identifier.uri.fl_str_mv |
https://repositorio.ufscar.br/handle/ufscar/10249 |
identifier_str_mv |
MORAIS, Dayane Vanessa. Produção de ficocianina por Aphanothece microscopica Nägeli a partir de vinhaça de cana-de-açúcar. 2018. Dissertação (Mestrado em Produção Vegetal e Bioprocessos Associados) – Universidade Federal de São Carlos, Araras, 2018. Disponível em: https://repositorio.ufscar.br/handle/ufscar/10249. |
url |
https://repositorio.ufscar.br/handle/ufscar/10249 |
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openAccess |
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Universidade Federal de São Carlos Câmpus Araras |
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Programa de Pós-Graduação em Produção Vegetal e Bioprocessos Associados - PPGPVBA-Ar |
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UFSCar |
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Universidade Federal de São Carlos Câmpus Araras |
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