Efeito da exposição à atrazina nas brânquias de curimbatá, Prochilodus lineatus (Teleósteo, Prochilodontidae)
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFSCAR |
Texto Completo: | https://repositorio.ufscar.br/handle/ufscar/1329 |
Resumo: | The herbicide atrazine is one of the most common pesticides found in the aquatic ecosystems all over the world. In this study the effects of atrazine, at sublethal concentrations, were evaluated on the gills of Prochilodus lineatus. Juveniles P. lineatus were divided in 4 groups: control and exposed to atrazine (2, 10 e 25 μg.L-1) for 48 hours or 14 days. After the experimental periods, blood samples were taken by caudal puncture for ion plasma analusis and the gills were removed and samples were fixed for morphological analysis (chloride cell immunohistochemistry, scanning electron microscopy, histochemistry of mucous cells and histopathology) or frozen for biochemical analysis (Na+/K+-ATPase and oxidative stress enzyme activity, GSH and determination of lipid hydroperoxidation). The activity of antioxidant enzymes (SOD, CAT e GST) e lipid peroxidation increased in fish of group exposed to 10 μg.L-1 for 14 days. Although there was an increase on plasma Na+ e Cl- and osmolality in fish exposed for 14 days it was not characterized as ionic imbalance as the Na+/Cl- ratio was similar in all groups. The total number of chloride cells decreased significantly only in the group exposed to 25 μg.l-1 of atrazine (48 h) and the CC density at epithelial surface and the fractional surface area of chloride cells increase in both treatments (48 h and 14 days) in fish exposed to 25 and 10 μg.l-1, respectively. The Na+/K+-ATPase activity was not altered in any experimental condition. The number of mucous cells containing sulfated mucins decreased. Hypertrophy and disruption of lamellar epithelium and vascular congestion were the most common injuries found in the gill tissue of P. lineatus and in fish exposed to 25 μg.l-1 atrazine (14 dias) the histopathological changes index was calculate as slightly to moderate. In conclusion, the tested concentrations, which are similar to those found in natural environments, may cause alterations in the gills of P. lienatus, so as not to endanger the vital functions of the gills and homeostasis of the animal. The changes observed in the gill of P. lineatus after atrazine exposure at tested concentrations, similar to that found in the natural environment, suggested that atrazine may affect the organ function. |
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Paulino, Marcelo GustavoFernandes, Marisa Narcisohttp://genos.cnpq.br:12010/dwlattes/owa/prc_imp_cv_int?f_cod=K4783604Y6http://lattes.cnpq.br/6503185330517924a4a1a785-aaa6-48ec-8e83-ec9c11993a012016-06-02T19:22:54Z2011-05-202016-06-02T19:22:54Z2011-03-03PAULINO, Marcelo Gustavo. Efeito da exposição à atrazina nas brânquias de curimbatá, Prochilodus lineatus (Teleósteo, Prochilodontidae). 2011. 118 f. Dissertação (Mestrado em Ciências Biológicas) - Universidade Federal de São Carlos, São Carlos, 2011.https://repositorio.ufscar.br/handle/ufscar/1329The herbicide atrazine is one of the most common pesticides found in the aquatic ecosystems all over the world. In this study the effects of atrazine, at sublethal concentrations, were evaluated on the gills of Prochilodus lineatus. Juveniles P. lineatus were divided in 4 groups: control and exposed to atrazine (2, 10 e 25 μg.L-1) for 48 hours or 14 days. After the experimental periods, blood samples were taken by caudal puncture for ion plasma analusis and the gills were removed and samples were fixed for morphological analysis (chloride cell immunohistochemistry, scanning electron microscopy, histochemistry of mucous cells and histopathology) or frozen for biochemical analysis (Na+/K+-ATPase and oxidative stress enzyme activity, GSH and determination of lipid hydroperoxidation). The activity of antioxidant enzymes (SOD, CAT e GST) e lipid peroxidation increased in fish of group exposed to 10 μg.L-1 for 14 days. Although there was an increase on plasma Na+ e Cl- and osmolality in fish exposed for 14 days it was not characterized as ionic imbalance as the Na+/Cl- ratio was similar in all groups. The total number of chloride cells decreased significantly only in the group exposed to 25 μg.l-1 of atrazine (48 h) and the CC density at epithelial surface and the fractional surface area of chloride cells increase in both treatments (48 h and 14 days) in fish exposed to 25 and 10 μg.l-1, respectively. The Na+/K+-ATPase activity was not altered in any experimental condition. The number of mucous cells containing sulfated mucins decreased. Hypertrophy and disruption of lamellar epithelium and vascular congestion were the most common injuries found in the gill tissue of P. lineatus and in fish exposed to 25 μg.l-1 atrazine (14 dias) the histopathological changes index was calculate as slightly to moderate. In conclusion, the tested concentrations, which are similar to those found in natural environments, may cause alterations in the gills of P. lienatus, so as not to endanger the vital functions of the gills and homeostasis of the animal. The changes observed in the gill of P. lineatus after atrazine exposure at tested concentrations, similar to that found in the natural environment, suggested that atrazine may affect the organ function.O herbicida atrazina é um dos pesticidas mais encontrados em ecossistemas aquáticos em todo o mundo. Nesse estudo os efeitos da atrazina, em concentrações subletais, foram avaliados nas brânquias de Prochilodus lineatus quanto aos aspectos morfo-funcionais do órgão. Exemplares juvenis de P. lineatus foram divididos em 4 grupos: controle e expostos à atrazina (2, 10 e 25 μg.L-1) por 48 horas ou 14 dias. Após os períodos experimentais, amostras de sangue foram obtidas por punção caudal para análise dos íons plasmáticos, as brânquias foram removidas e amostras fixadas para análise morfológica (imunohistoquímica de células-cloreto, microscopia eletrônica de varredura, histoquímica de células mucosas e histopatologia) ou congeladas para análises bioquímicas (atividade da enzima Na+/K+-ATPase e enzimas antioxidantes, GSH e determinação de hidroperóxidos de lipídios). A atividade das enzimas de defesa antioxidante (SOD, CAT e GST) e formação de hidroperóxidos de lipídeos aumentou no grupo exposto a 10 μg.L-1 por 14 dias. Apesar de ocorrer um aumento na Na+ e Cl- e na osmolaridade plasmática do grupo exposto por 14 dias não foi caracterizado desequilíbrio eletrolítico visto que a razão Na+/Cl- foi similar em todos os grupos. O número total de células-cloreto (CC) diminuiu significativamente apenas no grupo exposto a 25 μg.L-1 de atrazina (48 h) e houve um aumento da densidade das CCs na superfície epitelial e da área fracional das CCs em ambos os tratamentos (48 h e 14 dias) nas concentrações de 25 e 10 μg.L-1, respectivamente. A atividade da enzima Na+/K+-ATPase não foi alterada em nenhuma condição experimental. Em ambos os períodos houve uma diminuição no número de células mucosas contendo mucinas sulfatadas. Hipertrofia e ruptura do epitélio lamelar e congestão vascular foram as lesões mais comuns encontradas no tecido branquial em animais expostos a atrazina a 25 ugL-1 (14 dias) e o Índice de Alterações Histopatologicas foi calculado como leve a moderado. As alterações observadas nas brânquias de P. lineatus após a exposição a atrazina sugere que nas concentrações testadas, similares às encontradas em ambientes naturais, pode causar alterações moderadas nas brânquias e comprometer as funções do órgão.Universidade Federal de Sao Carlosapplication/pdfporUniversidade Federal de São CarlosPrograma Interinstitucional de Pós-Graduação em Ciências Fisiológicas - PIPGCFUFSCarBRPeixe - fisiologiaBrânquiasAtrazinaProchilodus lineatusCélulas cloretoHistopatologiaCIENCIAS BIOLOGICAS::FISIOLOGIAEfeito da exposição à atrazina nas brânquias de curimbatá, Prochilodus lineatus (Teleósteo, Prochilodontidae)info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis-1-15470807a-1e63-4591-8511-0cb223b337f2info:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFSCARinstname:Universidade Federal de São Carlos (UFSCAR)instacron:UFSCARORIGINAL3620.pdfapplication/pdf6241997https://repositorio.ufscar.br/bitstream/ufscar/1329/1/3620.pdfa8410d2f6c9083e06f888dd61a69934fMD51THUMBNAIL3620.pdf.jpg3620.pdf.jpgIM Thumbnailimage/jpeg6000https://repositorio.ufscar.br/bitstream/ufscar/1329/2/3620.pdf.jpg284eff3402cd047b3d48de90348da7a2MD52ufscar/13292023-09-18 18:30:41.903oai:repositorio.ufscar.br:ufscar/1329Repositório InstitucionalPUBhttps://repositorio.ufscar.br/oai/requestopendoar:43222023-09-18T18:30:41Repositório Institucional da UFSCAR - Universidade Federal de São Carlos (UFSCAR)false |
dc.title.por.fl_str_mv |
Efeito da exposição à atrazina nas brânquias de curimbatá, Prochilodus lineatus (Teleósteo, Prochilodontidae) |
title |
Efeito da exposição à atrazina nas brânquias de curimbatá, Prochilodus lineatus (Teleósteo, Prochilodontidae) |
spellingShingle |
Efeito da exposição à atrazina nas brânquias de curimbatá, Prochilodus lineatus (Teleósteo, Prochilodontidae) Paulino, Marcelo Gustavo Peixe - fisiologia Brânquias Atrazina Prochilodus lineatus Células cloreto Histopatologia CIENCIAS BIOLOGICAS::FISIOLOGIA |
title_short |
Efeito da exposição à atrazina nas brânquias de curimbatá, Prochilodus lineatus (Teleósteo, Prochilodontidae) |
title_full |
Efeito da exposição à atrazina nas brânquias de curimbatá, Prochilodus lineatus (Teleósteo, Prochilodontidae) |
title_fullStr |
Efeito da exposição à atrazina nas brânquias de curimbatá, Prochilodus lineatus (Teleósteo, Prochilodontidae) |
title_full_unstemmed |
Efeito da exposição à atrazina nas brânquias de curimbatá, Prochilodus lineatus (Teleósteo, Prochilodontidae) |
title_sort |
Efeito da exposição à atrazina nas brânquias de curimbatá, Prochilodus lineatus (Teleósteo, Prochilodontidae) |
author |
Paulino, Marcelo Gustavo |
author_facet |
Paulino, Marcelo Gustavo |
author_role |
author |
dc.contributor.authorlattes.por.fl_str_mv |
http://lattes.cnpq.br/6503185330517924 |
dc.contributor.author.fl_str_mv |
Paulino, Marcelo Gustavo |
dc.contributor.advisor1.fl_str_mv |
Fernandes, Marisa Narciso |
dc.contributor.advisor1Lattes.fl_str_mv |
http://genos.cnpq.br:12010/dwlattes/owa/prc_imp_cv_int?f_cod=K4783604Y6 |
dc.contributor.authorID.fl_str_mv |
a4a1a785-aaa6-48ec-8e83-ec9c11993a01 |
contributor_str_mv |
Fernandes, Marisa Narciso |
dc.subject.por.fl_str_mv |
Peixe - fisiologia Brânquias Atrazina Prochilodus lineatus Células cloreto Histopatologia |
topic |
Peixe - fisiologia Brânquias Atrazina Prochilodus lineatus Células cloreto Histopatologia CIENCIAS BIOLOGICAS::FISIOLOGIA |
dc.subject.cnpq.fl_str_mv |
CIENCIAS BIOLOGICAS::FISIOLOGIA |
description |
The herbicide atrazine is one of the most common pesticides found in the aquatic ecosystems all over the world. In this study the effects of atrazine, at sublethal concentrations, were evaluated on the gills of Prochilodus lineatus. Juveniles P. lineatus were divided in 4 groups: control and exposed to atrazine (2, 10 e 25 μg.L-1) for 48 hours or 14 days. After the experimental periods, blood samples were taken by caudal puncture for ion plasma analusis and the gills were removed and samples were fixed for morphological analysis (chloride cell immunohistochemistry, scanning electron microscopy, histochemistry of mucous cells and histopathology) or frozen for biochemical analysis (Na+/K+-ATPase and oxidative stress enzyme activity, GSH and determination of lipid hydroperoxidation). The activity of antioxidant enzymes (SOD, CAT e GST) e lipid peroxidation increased in fish of group exposed to 10 μg.L-1 for 14 days. Although there was an increase on plasma Na+ e Cl- and osmolality in fish exposed for 14 days it was not characterized as ionic imbalance as the Na+/Cl- ratio was similar in all groups. The total number of chloride cells decreased significantly only in the group exposed to 25 μg.l-1 of atrazine (48 h) and the CC density at epithelial surface and the fractional surface area of chloride cells increase in both treatments (48 h and 14 days) in fish exposed to 25 and 10 μg.l-1, respectively. The Na+/K+-ATPase activity was not altered in any experimental condition. The number of mucous cells containing sulfated mucins decreased. Hypertrophy and disruption of lamellar epithelium and vascular congestion were the most common injuries found in the gill tissue of P. lineatus and in fish exposed to 25 μg.l-1 atrazine (14 dias) the histopathological changes index was calculate as slightly to moderate. In conclusion, the tested concentrations, which are similar to those found in natural environments, may cause alterations in the gills of P. lienatus, so as not to endanger the vital functions of the gills and homeostasis of the animal. The changes observed in the gill of P. lineatus after atrazine exposure at tested concentrations, similar to that found in the natural environment, suggested that atrazine may affect the organ function. |
publishDate |
2011 |
dc.date.available.fl_str_mv |
2011-05-20 2016-06-02T19:22:54Z |
dc.date.issued.fl_str_mv |
2011-03-03 |
dc.date.accessioned.fl_str_mv |
2016-06-02T19:22:54Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
PAULINO, Marcelo Gustavo. Efeito da exposição à atrazina nas brânquias de curimbatá, Prochilodus lineatus (Teleósteo, Prochilodontidae). 2011. 118 f. Dissertação (Mestrado em Ciências Biológicas) - Universidade Federal de São Carlos, São Carlos, 2011. |
dc.identifier.uri.fl_str_mv |
https://repositorio.ufscar.br/handle/ufscar/1329 |
identifier_str_mv |
PAULINO, Marcelo Gustavo. Efeito da exposição à atrazina nas brânquias de curimbatá, Prochilodus lineatus (Teleósteo, Prochilodontidae). 2011. 118 f. Dissertação (Mestrado em Ciências Biológicas) - Universidade Federal de São Carlos, São Carlos, 2011. |
url |
https://repositorio.ufscar.br/handle/ufscar/1329 |
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por |
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por |
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-1 -1 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
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Universidade Federal de São Carlos |
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Programa Interinstitucional de Pós-Graduação em Ciências Fisiológicas - PIPGCF |
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UFSCar |
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BR |
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Universidade Federal de São Carlos |
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