Análise da sazonalidade e regionalidade em suco de laranja natural por ressonância magnética nuclear (RMN) e quimiometria

Detalhes bibliográficos
Autor(a) principal: Oliveira, Clayton Rodrigues de
Data de Publicação: 2017
Tipo de documento: Tese
Idioma: por
Título da fonte: Repositório Institucional da UFSCAR
Texto Completo: https://repositorio.ufscar.br/handle/ufscar/11372
Resumo: The Nuclear Magnetic Resonance (NMR) has been become increasingly helpful as an analytical tool to assess the food quality, specially to highlight fruit juice in the last few years. In this context, NMR has presented solutions to identify frauds such as addition of unauthorized or not allowed compounds in fruit juices, wines, honeys, spirits, coffees, olive oil, etc.. The development of databases is used for this purpose which enables to perform at the same time the quantification of large numbers of compounds into the samples, as well as to provide the information about origin, variety and type of fruit that were used throughout orange juice production. The purpose of this study was to evaluate the seasonality and regionality in fresh orange juice used to produce concentrated orange juice for exportation through the orange varieties hamlin, pera, natal, and valência (Citrus sinensis (L.) Osbeck). 1H NMR and 2D analysis enabled us to characterize more than 20 compounds in fresh orange juice, and also to quantify the content of sugars (sucrose, α and β-glucose, and fructose), acids (citric, malic, ascorbic, succinic, and γ-aminobutyric (GABA)), aminoacids (proline, arginine, alanine, threonine, and valine), ratio and ethanol. The seasonality effects were evaluated by using principal components analysis (PCA), where it was possible to determine that region 3 showed more explicity defined seasonality stages for the varieties pera, natal and valência across the 2014 harvest. In contrast, the hamlin type presented more defined seasonality stages in region 2 for 2014´s harvest. The 1H NMR allied to supervised analysis of Partial Least Squares Discriminant Analysis (PLS-DA) lead to development of models where it was possible to distinguish the year of harvest, type of orange (early or late), type of variety (hamlin, pera, natal or valência) and the region (1, 2 or 3) where the orange juice was obtained.
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spelling Oliveira, Clayton Rodrigues deFerreira, Antonio Gilbertohttp://lattes.cnpq.br/3676462220401452http://lattes.cnpq.br/7172297688805316cc4a4935-1c41-4408-b832-47a35d77f10a2019-05-06T18:04:31Z2019-05-06T18:04:31Z2017-10-27OLIVEIRA, Clayton Rodrigues de. Análise da sazonalidade e regionalidade em suco de laranja natural por ressonância magnética nuclear (RMN) e quimiometria. 2017. Tese (Doutorado em Química) – Universidade Federal de São Carlos, São Carlos, 2017. Disponível em: https://repositorio.ufscar.br/handle/ufscar/11372.https://repositorio.ufscar.br/handle/ufscar/11372The Nuclear Magnetic Resonance (NMR) has been become increasingly helpful as an analytical tool to assess the food quality, specially to highlight fruit juice in the last few years. In this context, NMR has presented solutions to identify frauds such as addition of unauthorized or not allowed compounds in fruit juices, wines, honeys, spirits, coffees, olive oil, etc.. The development of databases is used for this purpose which enables to perform at the same time the quantification of large numbers of compounds into the samples, as well as to provide the information about origin, variety and type of fruit that were used throughout orange juice production. The purpose of this study was to evaluate the seasonality and regionality in fresh orange juice used to produce concentrated orange juice for exportation through the orange varieties hamlin, pera, natal, and valência (Citrus sinensis (L.) Osbeck). 1H NMR and 2D analysis enabled us to characterize more than 20 compounds in fresh orange juice, and also to quantify the content of sugars (sucrose, α and β-glucose, and fructose), acids (citric, malic, ascorbic, succinic, and γ-aminobutyric (GABA)), aminoacids (proline, arginine, alanine, threonine, and valine), ratio and ethanol. The seasonality effects were evaluated by using principal components analysis (PCA), where it was possible to determine that region 3 showed more explicity defined seasonality stages for the varieties pera, natal and valência across the 2014 harvest. In contrast, the hamlin type presented more defined seasonality stages in region 2 for 2014´s harvest. The 1H NMR allied to supervised analysis of Partial Least Squares Discriminant Analysis (PLS-DA) lead to development of models where it was possible to distinguish the year of harvest, type of orange (early or late), type of variety (hamlin, pera, natal or valência) and the region (1, 2 or 3) where the orange juice was obtained.Nos últimos anos a Ressonância Magnética Nuclear (RMN) vem se tornando uma ferramenta analítica cada vez mais útil para avaliar a qualidade de alimentos, em especial suco de frutas. Neste contexto, a RMN tem apresentado soluções para identificar fraudes como à adição de substâncias indevidas ou não permitidas em suco de frutas, vinhos, méis, cachaça, café, azeite de oliva, etc.. A construção de bancos de dados é usada com este proposito, a qual permite quantificar simultaneamente uma grande quantidade de substâncias presente nas amostras, além de fornecer informações sobre origem, variedade e tipo de fruta utilizada na produção de um suco. O objetivo desse trabalho foi avaliar a sazonalidade e regionalidade em suco de laranja integral usado para produção de suco concentrado destinado à exportação, através das variedades de laranja hamlin, pera, natal e valência (Citrus sinensis (L.) Osbeck). As técnicas de RMN de 1H e 2D nos permitiu caracterizar mais de 20 metabólitos presentes no suco de laranja integral, além de quantificar o conteúdo de açúcares (sacarose, α e β-glicose e frutose), ácidos (cítrico, málico, ascórbico, succínico e γ-aminobutírico (GABA)); aminoácidos (prolina, arginina, alanina, treonina e valina), ratio e etanol. Os efeitos da sazonalidade foram avaliados através das análises por componentes principais (PCA), onde foi possível constatar que a região 3 foi aquela que apresentou os períodos sazonais mais bem definidos ao longo das safras 2014 para as variedades pera, natal e valência. Ao contrario das demais variedades, a variedade hamlin teve os períodos sazonais mais bem definidos na região 2 ao longo da safra de 2014. Os dados de RMN de 1H aliados às análises supervisionadas de Partial Least Squares Discriminant Analysis (PLS-DA) possibilitaram construir modelos onde foi possível distinguir o ano da safra, o tipo de laranja (precoce ou tardia), tipo de variedade (hamlin, pera, natal ou valência) e a região (1, 2 ou 3) de onde o suco de laranja foi obtido.Não recebi financiamentoporUniversidade Federal de São CarlosCâmpus São CarlosPrograma de Pós-Graduação em Química - PPGQUFSCarSuco de laranjaRessonância magnética nuclearQuimiometriaOrange juiceNuclear magnetic resonanceChemometricsCIENCIAS EXATAS E DA TERRA::QUIMICA::QUIMICA ORGANICA::QUIMICA DOS PRODUTOS NATURAISAnálise da sazonalidade e regionalidade em suco de laranja natural por ressonância magnética nuclear (RMN) e quimiometriainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesis12 meses após a data da defesa600ddf683dd-9e54-4d02-b23f-b33d9b4d289finfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFSCARinstname:Universidade Federal de São Carlos (UFSCAR)instacron:UFSCARORIGINALTese CRO-dez18_defesa_corrigida.pdfTese CRO-dez18_defesa_corrigida.pdfTeseapplication/pdf5554335https://repositorio.ufscar.br/bitstream/ufscar/11372/3/Tese%20CRO-dez18_defesa_corrigida.pdf6fe9171ad449d2b1314a1c412602e883MD53LICENSElicense.txtlicense.txttext/plain; charset=utf-81957https://repositorio.ufscar.br/bitstream/ufscar/11372/6/license.txtae0398b6f8b235e40ad82cba6c50031dMD56TEXTTese CRO-dez18_defesa_corrigida.pdf.txtTese CRO-dez18_defesa_corrigida.pdf.txtExtracted texttext/plain173955https://repositorio.ufscar.br/bitstream/ufscar/11372/7/Tese%20CRO-dez18_defesa_corrigida.pdf.txt630aded0788f21b771b966c5997849cbMD57THUMBNAILTese CRO-dez18_defesa_corrigida.pdf.jpgTese CRO-dez18_defesa_corrigida.pdf.jpgIM Thumbnailimage/jpeg8623https://repositorio.ufscar.br/bitstream/ufscar/11372/8/Tese%20CRO-dez18_defesa_corrigida.pdf.jpg2899cf92992704482208b589738a3966MD58ufscar/113722023-09-18 18:31:22.591oai:repositorio.ufscar.br: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Repositório InstitucionalPUBhttps://repositorio.ufscar.br/oai/requestopendoar:43222023-09-18T18:31:22Repositório Institucional da UFSCAR - Universidade Federal de São Carlos (UFSCAR)false
dc.title.por.fl_str_mv Análise da sazonalidade e regionalidade em suco de laranja natural por ressonância magnética nuclear (RMN) e quimiometria
title Análise da sazonalidade e regionalidade em suco de laranja natural por ressonância magnética nuclear (RMN) e quimiometria
spellingShingle Análise da sazonalidade e regionalidade em suco de laranja natural por ressonância magnética nuclear (RMN) e quimiometria
Oliveira, Clayton Rodrigues de
Suco de laranja
Ressonância magnética nuclear
Quimiometria
Orange juice
Nuclear magnetic resonance
Chemometrics
CIENCIAS EXATAS E DA TERRA::QUIMICA::QUIMICA ORGANICA::QUIMICA DOS PRODUTOS NATURAIS
title_short Análise da sazonalidade e regionalidade em suco de laranja natural por ressonância magnética nuclear (RMN) e quimiometria
title_full Análise da sazonalidade e regionalidade em suco de laranja natural por ressonância magnética nuclear (RMN) e quimiometria
title_fullStr Análise da sazonalidade e regionalidade em suco de laranja natural por ressonância magnética nuclear (RMN) e quimiometria
title_full_unstemmed Análise da sazonalidade e regionalidade em suco de laranja natural por ressonância magnética nuclear (RMN) e quimiometria
title_sort Análise da sazonalidade e regionalidade em suco de laranja natural por ressonância magnética nuclear (RMN) e quimiometria
author Oliveira, Clayton Rodrigues de
author_facet Oliveira, Clayton Rodrigues de
author_role author
dc.contributor.authorlattes.por.fl_str_mv http://lattes.cnpq.br/7172297688805316
dc.contributor.author.fl_str_mv Oliveira, Clayton Rodrigues de
dc.contributor.advisor1.fl_str_mv Ferreira, Antonio Gilberto
dc.contributor.advisor1Lattes.fl_str_mv http://lattes.cnpq.br/3676462220401452
dc.contributor.authorID.fl_str_mv cc4a4935-1c41-4408-b832-47a35d77f10a
contributor_str_mv Ferreira, Antonio Gilberto
dc.subject.por.fl_str_mv Suco de laranja
Ressonância magnética nuclear
Quimiometria
topic Suco de laranja
Ressonância magnética nuclear
Quimiometria
Orange juice
Nuclear magnetic resonance
Chemometrics
CIENCIAS EXATAS E DA TERRA::QUIMICA::QUIMICA ORGANICA::QUIMICA DOS PRODUTOS NATURAIS
dc.subject.eng.fl_str_mv Orange juice
Nuclear magnetic resonance
Chemometrics
dc.subject.cnpq.fl_str_mv CIENCIAS EXATAS E DA TERRA::QUIMICA::QUIMICA ORGANICA::QUIMICA DOS PRODUTOS NATURAIS
description The Nuclear Magnetic Resonance (NMR) has been become increasingly helpful as an analytical tool to assess the food quality, specially to highlight fruit juice in the last few years. In this context, NMR has presented solutions to identify frauds such as addition of unauthorized or not allowed compounds in fruit juices, wines, honeys, spirits, coffees, olive oil, etc.. The development of databases is used for this purpose which enables to perform at the same time the quantification of large numbers of compounds into the samples, as well as to provide the information about origin, variety and type of fruit that were used throughout orange juice production. The purpose of this study was to evaluate the seasonality and regionality in fresh orange juice used to produce concentrated orange juice for exportation through the orange varieties hamlin, pera, natal, and valência (Citrus sinensis (L.) Osbeck). 1H NMR and 2D analysis enabled us to characterize more than 20 compounds in fresh orange juice, and also to quantify the content of sugars (sucrose, α and β-glucose, and fructose), acids (citric, malic, ascorbic, succinic, and γ-aminobutyric (GABA)), aminoacids (proline, arginine, alanine, threonine, and valine), ratio and ethanol. The seasonality effects were evaluated by using principal components analysis (PCA), where it was possible to determine that region 3 showed more explicity defined seasonality stages for the varieties pera, natal and valência across the 2014 harvest. In contrast, the hamlin type presented more defined seasonality stages in region 2 for 2014´s harvest. The 1H NMR allied to supervised analysis of Partial Least Squares Discriminant Analysis (PLS-DA) lead to development of models where it was possible to distinguish the year of harvest, type of orange (early or late), type of variety (hamlin, pera, natal or valência) and the region (1, 2 or 3) where the orange juice was obtained.
publishDate 2017
dc.date.issued.fl_str_mv 2017-10-27
dc.date.accessioned.fl_str_mv 2019-05-06T18:04:31Z
dc.date.available.fl_str_mv 2019-05-06T18:04:31Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/doctoralThesis
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status_str publishedVersion
dc.identifier.citation.fl_str_mv OLIVEIRA, Clayton Rodrigues de. Análise da sazonalidade e regionalidade em suco de laranja natural por ressonância magnética nuclear (RMN) e quimiometria. 2017. Tese (Doutorado em Química) – Universidade Federal de São Carlos, São Carlos, 2017. Disponível em: https://repositorio.ufscar.br/handle/ufscar/11372.
dc.identifier.uri.fl_str_mv https://repositorio.ufscar.br/handle/ufscar/11372
identifier_str_mv OLIVEIRA, Clayton Rodrigues de. Análise da sazonalidade e regionalidade em suco de laranja natural por ressonância magnética nuclear (RMN) e quimiometria. 2017. Tese (Doutorado em Química) – Universidade Federal de São Carlos, São Carlos, 2017. Disponível em: https://repositorio.ufscar.br/handle/ufscar/11372.
url https://repositorio.ufscar.br/handle/ufscar/11372
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dc.publisher.none.fl_str_mv Universidade Federal de São Carlos
Câmpus São Carlos
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dc.publisher.initials.fl_str_mv UFSCar
publisher.none.fl_str_mv Universidade Federal de São Carlos
Câmpus São Carlos
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