Análise da sazonalidade e regionalidade em suco de laranja natural por ressonância magnética nuclear (RMN) e quimiometria
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Tipo de documento: | Tese |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFSCAR |
Texto Completo: | https://repositorio.ufscar.br/handle/ufscar/11372 |
Resumo: | The Nuclear Magnetic Resonance (NMR) has been become increasingly helpful as an analytical tool to assess the food quality, specially to highlight fruit juice in the last few years. In this context, NMR has presented solutions to identify frauds such as addition of unauthorized or not allowed compounds in fruit juices, wines, honeys, spirits, coffees, olive oil, etc.. The development of databases is used for this purpose which enables to perform at the same time the quantification of large numbers of compounds into the samples, as well as to provide the information about origin, variety and type of fruit that were used throughout orange juice production. The purpose of this study was to evaluate the seasonality and regionality in fresh orange juice used to produce concentrated orange juice for exportation through the orange varieties hamlin, pera, natal, and valência (Citrus sinensis (L.) Osbeck). 1H NMR and 2D analysis enabled us to characterize more than 20 compounds in fresh orange juice, and also to quantify the content of sugars (sucrose, α and β-glucose, and fructose), acids (citric, malic, ascorbic, succinic, and γ-aminobutyric (GABA)), aminoacids (proline, arginine, alanine, threonine, and valine), ratio and ethanol. The seasonality effects were evaluated by using principal components analysis (PCA), where it was possible to determine that region 3 showed more explicity defined seasonality stages for the varieties pera, natal and valência across the 2014 harvest. In contrast, the hamlin type presented more defined seasonality stages in region 2 for 2014´s harvest. The 1H NMR allied to supervised analysis of Partial Least Squares Discriminant Analysis (PLS-DA) lead to development of models where it was possible to distinguish the year of harvest, type of orange (early or late), type of variety (hamlin, pera, natal or valência) and the region (1, 2 or 3) where the orange juice was obtained. |
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Oliveira, Clayton Rodrigues deFerreira, Antonio Gilbertohttp://lattes.cnpq.br/3676462220401452http://lattes.cnpq.br/7172297688805316cc4a4935-1c41-4408-b832-47a35d77f10a2019-05-06T18:04:31Z2019-05-06T18:04:31Z2017-10-27OLIVEIRA, Clayton Rodrigues de. Análise da sazonalidade e regionalidade em suco de laranja natural por ressonância magnética nuclear (RMN) e quimiometria. 2017. Tese (Doutorado em Química) – Universidade Federal de São Carlos, São Carlos, 2017. Disponível em: https://repositorio.ufscar.br/handle/ufscar/11372.https://repositorio.ufscar.br/handle/ufscar/11372The Nuclear Magnetic Resonance (NMR) has been become increasingly helpful as an analytical tool to assess the food quality, specially to highlight fruit juice in the last few years. In this context, NMR has presented solutions to identify frauds such as addition of unauthorized or not allowed compounds in fruit juices, wines, honeys, spirits, coffees, olive oil, etc.. The development of databases is used for this purpose which enables to perform at the same time the quantification of large numbers of compounds into the samples, as well as to provide the information about origin, variety and type of fruit that were used throughout orange juice production. The purpose of this study was to evaluate the seasonality and regionality in fresh orange juice used to produce concentrated orange juice for exportation through the orange varieties hamlin, pera, natal, and valência (Citrus sinensis (L.) Osbeck). 1H NMR and 2D analysis enabled us to characterize more than 20 compounds in fresh orange juice, and also to quantify the content of sugars (sucrose, α and β-glucose, and fructose), acids (citric, malic, ascorbic, succinic, and γ-aminobutyric (GABA)), aminoacids (proline, arginine, alanine, threonine, and valine), ratio and ethanol. The seasonality effects were evaluated by using principal components analysis (PCA), where it was possible to determine that region 3 showed more explicity defined seasonality stages for the varieties pera, natal and valência across the 2014 harvest. In contrast, the hamlin type presented more defined seasonality stages in region 2 for 2014´s harvest. The 1H NMR allied to supervised analysis of Partial Least Squares Discriminant Analysis (PLS-DA) lead to development of models where it was possible to distinguish the year of harvest, type of orange (early or late), type of variety (hamlin, pera, natal or valência) and the region (1, 2 or 3) where the orange juice was obtained.Nos últimos anos a Ressonância Magnética Nuclear (RMN) vem se tornando uma ferramenta analítica cada vez mais útil para avaliar a qualidade de alimentos, em especial suco de frutas. Neste contexto, a RMN tem apresentado soluções para identificar fraudes como à adição de substâncias indevidas ou não permitidas em suco de frutas, vinhos, méis, cachaça, café, azeite de oliva, etc.. A construção de bancos de dados é usada com este proposito, a qual permite quantificar simultaneamente uma grande quantidade de substâncias presente nas amostras, além de fornecer informações sobre origem, variedade e tipo de fruta utilizada na produção de um suco. O objetivo desse trabalho foi avaliar a sazonalidade e regionalidade em suco de laranja integral usado para produção de suco concentrado destinado à exportação, através das variedades de laranja hamlin, pera, natal e valência (Citrus sinensis (L.) Osbeck). As técnicas de RMN de 1H e 2D nos permitiu caracterizar mais de 20 metabólitos presentes no suco de laranja integral, além de quantificar o conteúdo de açúcares (sacarose, α e β-glicose e frutose), ácidos (cítrico, málico, ascórbico, succínico e γ-aminobutírico (GABA)); aminoácidos (prolina, arginina, alanina, treonina e valina), ratio e etanol. Os efeitos da sazonalidade foram avaliados através das análises por componentes principais (PCA), onde foi possível constatar que a região 3 foi aquela que apresentou os períodos sazonais mais bem definidos ao longo das safras 2014 para as variedades pera, natal e valência. Ao contrario das demais variedades, a variedade hamlin teve os períodos sazonais mais bem definidos na região 2 ao longo da safra de 2014. Os dados de RMN de 1H aliados às análises supervisionadas de Partial Least Squares Discriminant Analysis (PLS-DA) possibilitaram construir modelos onde foi possível distinguir o ano da safra, o tipo de laranja (precoce ou tardia), tipo de variedade (hamlin, pera, natal ou valência) e a região (1, 2 ou 3) de onde o suco de laranja foi obtido.Não recebi financiamentoporUniversidade Federal de São CarlosCâmpus São CarlosPrograma de Pós-Graduação em Química - PPGQUFSCarSuco de laranjaRessonância magnética nuclearQuimiometriaOrange juiceNuclear magnetic resonanceChemometricsCIENCIAS EXATAS E DA TERRA::QUIMICA::QUIMICA ORGANICA::QUIMICA DOS PRODUTOS NATURAISAnálise da sazonalidade e regionalidade em suco de laranja natural por ressonância magnética nuclear (RMN) e quimiometriainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesis12 meses após a data da defesa600ddf683dd-9e54-4d02-b23f-b33d9b4d289finfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFSCARinstname:Universidade Federal de São Carlos (UFSCAR)instacron:UFSCARORIGINALTese CRO-dez18_defesa_corrigida.pdfTese CRO-dez18_defesa_corrigida.pdfTeseapplication/pdf5554335https://repositorio.ufscar.br/bitstream/ufscar/11372/3/Tese%20CRO-dez18_defesa_corrigida.pdf6fe9171ad449d2b1314a1c412602e883MD53LICENSElicense.txtlicense.txttext/plain; charset=utf-81957https://repositorio.ufscar.br/bitstream/ufscar/11372/6/license.txtae0398b6f8b235e40ad82cba6c50031dMD56TEXTTese CRO-dez18_defesa_corrigida.pdf.txtTese CRO-dez18_defesa_corrigida.pdf.txtExtracted texttext/plain173955https://repositorio.ufscar.br/bitstream/ufscar/11372/7/Tese%20CRO-dez18_defesa_corrigida.pdf.txt630aded0788f21b771b966c5997849cbMD57THUMBNAILTese CRO-dez18_defesa_corrigida.pdf.jpgTese CRO-dez18_defesa_corrigida.pdf.jpgIM Thumbnailimage/jpeg8623https://repositorio.ufscar.br/bitstream/ufscar/11372/8/Tese%20CRO-dez18_defesa_corrigida.pdf.jpg2899cf92992704482208b589738a3966MD58ufscar/113722023-09-18 18:31:22.591oai:repositorio.ufscar.br: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Repositório InstitucionalPUBhttps://repositorio.ufscar.br/oai/requestopendoar:43222023-09-18T18:31:22Repositório Institucional da UFSCAR - Universidade Federal de São Carlos (UFSCAR)false |
dc.title.por.fl_str_mv |
Análise da sazonalidade e regionalidade em suco de laranja natural por ressonância magnética nuclear (RMN) e quimiometria |
title |
Análise da sazonalidade e regionalidade em suco de laranja natural por ressonância magnética nuclear (RMN) e quimiometria |
spellingShingle |
Análise da sazonalidade e regionalidade em suco de laranja natural por ressonância magnética nuclear (RMN) e quimiometria Oliveira, Clayton Rodrigues de Suco de laranja Ressonância magnética nuclear Quimiometria Orange juice Nuclear magnetic resonance Chemometrics CIENCIAS EXATAS E DA TERRA::QUIMICA::QUIMICA ORGANICA::QUIMICA DOS PRODUTOS NATURAIS |
title_short |
Análise da sazonalidade e regionalidade em suco de laranja natural por ressonância magnética nuclear (RMN) e quimiometria |
title_full |
Análise da sazonalidade e regionalidade em suco de laranja natural por ressonância magnética nuclear (RMN) e quimiometria |
title_fullStr |
Análise da sazonalidade e regionalidade em suco de laranja natural por ressonância magnética nuclear (RMN) e quimiometria |
title_full_unstemmed |
Análise da sazonalidade e regionalidade em suco de laranja natural por ressonância magnética nuclear (RMN) e quimiometria |
title_sort |
Análise da sazonalidade e regionalidade em suco de laranja natural por ressonância magnética nuclear (RMN) e quimiometria |
author |
Oliveira, Clayton Rodrigues de |
author_facet |
Oliveira, Clayton Rodrigues de |
author_role |
author |
dc.contributor.authorlattes.por.fl_str_mv |
http://lattes.cnpq.br/7172297688805316 |
dc.contributor.author.fl_str_mv |
Oliveira, Clayton Rodrigues de |
dc.contributor.advisor1.fl_str_mv |
Ferreira, Antonio Gilberto |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/3676462220401452 |
dc.contributor.authorID.fl_str_mv |
cc4a4935-1c41-4408-b832-47a35d77f10a |
contributor_str_mv |
Ferreira, Antonio Gilberto |
dc.subject.por.fl_str_mv |
Suco de laranja Ressonância magnética nuclear Quimiometria |
topic |
Suco de laranja Ressonância magnética nuclear Quimiometria Orange juice Nuclear magnetic resonance Chemometrics CIENCIAS EXATAS E DA TERRA::QUIMICA::QUIMICA ORGANICA::QUIMICA DOS PRODUTOS NATURAIS |
dc.subject.eng.fl_str_mv |
Orange juice Nuclear magnetic resonance Chemometrics |
dc.subject.cnpq.fl_str_mv |
CIENCIAS EXATAS E DA TERRA::QUIMICA::QUIMICA ORGANICA::QUIMICA DOS PRODUTOS NATURAIS |
description |
The Nuclear Magnetic Resonance (NMR) has been become increasingly helpful as an analytical tool to assess the food quality, specially to highlight fruit juice in the last few years. In this context, NMR has presented solutions to identify frauds such as addition of unauthorized or not allowed compounds in fruit juices, wines, honeys, spirits, coffees, olive oil, etc.. The development of databases is used for this purpose which enables to perform at the same time the quantification of large numbers of compounds into the samples, as well as to provide the information about origin, variety and type of fruit that were used throughout orange juice production. The purpose of this study was to evaluate the seasonality and regionality in fresh orange juice used to produce concentrated orange juice for exportation through the orange varieties hamlin, pera, natal, and valência (Citrus sinensis (L.) Osbeck). 1H NMR and 2D analysis enabled us to characterize more than 20 compounds in fresh orange juice, and also to quantify the content of sugars (sucrose, α and β-glucose, and fructose), acids (citric, malic, ascorbic, succinic, and γ-aminobutyric (GABA)), aminoacids (proline, arginine, alanine, threonine, and valine), ratio and ethanol. The seasonality effects were evaluated by using principal components analysis (PCA), where it was possible to determine that region 3 showed more explicity defined seasonality stages for the varieties pera, natal and valência across the 2014 harvest. In contrast, the hamlin type presented more defined seasonality stages in region 2 for 2014´s harvest. The 1H NMR allied to supervised analysis of Partial Least Squares Discriminant Analysis (PLS-DA) lead to development of models where it was possible to distinguish the year of harvest, type of orange (early or late), type of variety (hamlin, pera, natal or valência) and the region (1, 2 or 3) where the orange juice was obtained. |
publishDate |
2017 |
dc.date.issued.fl_str_mv |
2017-10-27 |
dc.date.accessioned.fl_str_mv |
2019-05-06T18:04:31Z |
dc.date.available.fl_str_mv |
2019-05-06T18:04:31Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/doctoralThesis |
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doctoralThesis |
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publishedVersion |
dc.identifier.citation.fl_str_mv |
OLIVEIRA, Clayton Rodrigues de. Análise da sazonalidade e regionalidade em suco de laranja natural por ressonância magnética nuclear (RMN) e quimiometria. 2017. Tese (Doutorado em Química) – Universidade Federal de São Carlos, São Carlos, 2017. Disponível em: https://repositorio.ufscar.br/handle/ufscar/11372. |
dc.identifier.uri.fl_str_mv |
https://repositorio.ufscar.br/handle/ufscar/11372 |
identifier_str_mv |
OLIVEIRA, Clayton Rodrigues de. Análise da sazonalidade e regionalidade em suco de laranja natural por ressonância magnética nuclear (RMN) e quimiometria. 2017. Tese (Doutorado em Química) – Universidade Federal de São Carlos, São Carlos, 2017. Disponível em: https://repositorio.ufscar.br/handle/ufscar/11372. |
url |
https://repositorio.ufscar.br/handle/ufscar/11372 |
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openAccess |
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Universidade Federal de São Carlos Câmpus São Carlos |
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Programa de Pós-Graduação em Química - PPGQ |
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UFSCar |
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Universidade Federal de São Carlos Câmpus São Carlos |
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