Obtenção de nanocelulose bacteriana de kombucha
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFSCAR |
Texto Completo: | https://repositorio.ufscar.br/handle/ufscar/13522 |
Resumo: | In the last years, sustainable materials that provide less environmental impact are gaining special attention. In this context, cellulose, a natural and abundant polymer from several renewable vegetable and microbial sources, is being highlighted in related literature. Bacterial cellulose (BC) from microbial sources presents several advantageous properties as lignin and hemicellulose absence, high resistance to traction, elasticity, durability, and a high degree of crystallinity. Also, BC is biodegradable, non-toxic, and biocompatible. A less explored source of BC includes Kombucha membranes (KM), a by-product of Kombucha's ancient beverage. KM are cellulosic pellicles derived from the fermentation of black or green tea broth. Thus, the main objective of this study was the production and characterization of the physical and chemical properties of BC obtained from Kombucha’s bacterial cellulose membranes (KBCM) based on the fermentation of green tea broth with added sucrose. We also aimed at the production of bacterial nanocellulose from Kombucha beverage (BNKB) via a hydrolytic reaction. KBCM was produced using a symbiotic consortium of bacteria and yeast during the fermentation of green tea broth and sucrose inoculated with an initial KBCM solution and fermented at 35°C for 21 days. After, the KBCM solution was purified using distilled water and sodium hydroxide (NaOH). BKNB were extracted via KBCM acid hydrolysis with 64% sulfuric acid at 50°C, and reaction time variation, followed by dialysis processes until reach a neutral pH value. KBCM were analyzed before and after the purification process using atomic force microscopy (AFM), scanning electron microscopy (SEM), energy-dispersive spectroscopy (EDS), Fourier transform infrared (FTIR) spectroscopy, and X-ray diffraction (XRD). Suspensions containing BNKB were characterized by AFM only. KBCM before and after purification were characterized by the techniques of atomic force microscopy (AFM), scanning electron microscopy (SEM), dispersive energy spectroscopy (EDS), infrared absorption spectroscopy with Fourier transform (FTIR) and Diffraction X-ray (XRD). BNKB suspensions were characterized only by AFM. AFM and SEM analyzes revealed the morphology of the BNKB structures in long fibrous chains, which were clustered and interlaced. The relative intensities by XRD varied after the purification process, indicating a possible change in crystallinity. The presence of carbon and oxygen elements in KBCM before and after purification were also confirmed by EDS. Thus, it is concluded that through the mentioned characteristics and the BNKB morphology, they offer resistance in their cellulosic chains during the acid attack, which can be observed by the AFM images, due to the presence of curvatures in the KC nanofibrils, which by time it leverages it as a promising source of raw material for the production of new sustainable materials. |
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Góes, Thaís Soares deSilva, Adriana de Oliveira Delgadohttp://lattes.cnpq.br/2707979913713089Menezes, Aparecido Junior dehttp://lattes.cnpq.br/0484426340349483http://lattes.cnpq.br/035725658798346475c1544b-bd89-4d82-80df-8dd146193ee52020-12-04T16:37:51Z2020-12-04T16:37:51Z2020-03-24GÓES, Thaís Soares de. Obtenção de nanocelulose bacteriana de kombucha. 2020. Dissertação (Mestrado em Ciência dos Materiais) – Universidade Federal de São Carlos, Sorocaba, 2020. Disponível em: https://repositorio.ufscar.br/handle/ufscar/13522.https://repositorio.ufscar.br/handle/ufscar/13522In the last years, sustainable materials that provide less environmental impact are gaining special attention. In this context, cellulose, a natural and abundant polymer from several renewable vegetable and microbial sources, is being highlighted in related literature. Bacterial cellulose (BC) from microbial sources presents several advantageous properties as lignin and hemicellulose absence, high resistance to traction, elasticity, durability, and a high degree of crystallinity. Also, BC is biodegradable, non-toxic, and biocompatible. A less explored source of BC includes Kombucha membranes (KM), a by-product of Kombucha's ancient beverage. KM are cellulosic pellicles derived from the fermentation of black or green tea broth. Thus, the main objective of this study was the production and characterization of the physical and chemical properties of BC obtained from Kombucha’s bacterial cellulose membranes (KBCM) based on the fermentation of green tea broth with added sucrose. We also aimed at the production of bacterial nanocellulose from Kombucha beverage (BNKB) via a hydrolytic reaction. KBCM was produced using a symbiotic consortium of bacteria and yeast during the fermentation of green tea broth and sucrose inoculated with an initial KBCM solution and fermented at 35°C for 21 days. After, the KBCM solution was purified using distilled water and sodium hydroxide (NaOH). BKNB were extracted via KBCM acid hydrolysis with 64% sulfuric acid at 50°C, and reaction time variation, followed by dialysis processes until reach a neutral pH value. KBCM were analyzed before and after the purification process using atomic force microscopy (AFM), scanning electron microscopy (SEM), energy-dispersive spectroscopy (EDS), Fourier transform infrared (FTIR) spectroscopy, and X-ray diffraction (XRD). Suspensions containing BNKB were characterized by AFM only. KBCM before and after purification were characterized by the techniques of atomic force microscopy (AFM), scanning electron microscopy (SEM), dispersive energy spectroscopy (EDS), infrared absorption spectroscopy with Fourier transform (FTIR) and Diffraction X-ray (XRD). BNKB suspensions were characterized only by AFM. AFM and SEM analyzes revealed the morphology of the BNKB structures in long fibrous chains, which were clustered and interlaced. The relative intensities by XRD varied after the purification process, indicating a possible change in crystallinity. The presence of carbon and oxygen elements in KBCM before and after purification were also confirmed by EDS. Thus, it is concluded that through the mentioned characteristics and the BNKB morphology, they offer resistance in their cellulosic chains during the acid attack, which can be observed by the AFM images, due to the presence of curvatures in the KC nanofibrils, which by time it leverages it as a promising source of raw material for the production of new sustainable materials.Atenção especial tem sido dada nos últimos anos à busca por materiais sustentáveis que ofereçam menores impactos ao meio ambiente. Dessa forma, destaca-se a celulose, polímero natural abundante e presente em diferentes fontes renováveis, sejam vegetais ou microbianas. As últimas são capazes de produzir a chamada celulose bacteriana (CB), com vantagens como: ausência de lignina e hemiceluloses, alta resistência à tração, elasticidade, durabilidade e alta cristalinidade, além de ser biodegradável, atóxica e biocompatível. As membranas de Kombucha (KC), são fontes de CB ainda pouco exploradas. A KC, é subproduto de bebida milenar, consideradas películas celulósicas que podem ser obtidas por meio da fermentação de chá preto ou verde. Com isso, o objetivo deste estudo foi a produção e a caracterização das propriedades físicas e químicas de CB, a partir das membranas de celulose bacteriana de Kombucha (MCBK) cultivadas em chá verde e sacarose, além da obtenção de nanocelulose bacteriana de Kombucha (NCCB), via reação hidrolítica. As MCBK foram produzidas por meio de um consórcio simbiótico de leveduras e bactérias, cultivadas pela fermentação de chá verde e sacarose, a partir de um pré-inócúlo da própria MCBK, mantidos em estufa a 35°C por 21 dias, os quais foram purificadas com água destilada e hidróxido de sódio (NaOH) posteriormente. Para as suspensões de NCCB, estas foram obtidas mediante reação de hidrólise ácida das MCBK, com ácido sulfúrico (H2SO4) a 64% e 50°C, variando o tempo reacional, seguida do processo de diálise até pH neutro. As MCBK antes e após purificação foram caracterizadas mediante as técnicas de microscopia de força atômica (AFM), microscopia eletrônica de varredura (MEV), espectroscopia de energia dispersiva (EDS), espectroscopia de absorção no infravermelho com transformada de Fourier (FTIR) e Difração de Raio-X (DRX). As suspensões de NCCB foram caracterizadas apenas por AFM. Análises de AFM e MEV revelaram a morfologia das estruturas das MCBK em longas cadeias fibrosas, que se apresentaram aglomeradas e entrelaçadas. As intensidades relativas por DRX variaram após o processo de purificação, indicando possível alteração na cristalinidade. A presença dos elementos carbono e oxigênio nas MCBK antes e após purificação também foram confirmados por EDS. Assim, conclui-se que por meio da caraterizações mencionadas e pela morfologia das NCCB, estas oferecem resistência em suas cadeias celulósicas durante o ataque ácido, que pode ser observado pelas imagens de AFM, devido a presença de curvaturas nas nanofibrilas de KC, que por vez a potencializa como fonte promissora de matéria-prima, para produção de novos materiais sustentáveis.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)88882.426827/2019-01porUniversidade Federal de São CarlosCâmpus SorocabaPrograma de Pós-Graduação em Ciência dos Materiais - PPGCM-SoUFSCarAttribution-NonCommercial-NoDerivs 3.0 Brazilhttp://creativecommons.org/licenses/by-nc-nd/3.0/br/info:eu-repo/semantics/openAccessCelulose bacterianaBiopolímeroFontes renováveisNanociênciaNanoscienceRenewable sourcesBacterial celluloseBiopolymersCIENCIAS EXATAS E DA TERRA::QUIMICAObtenção de nanocelulose bacteriana de kombuchaObtaining of bacterial nanocellulose from kombuchainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis600600ed0daaa2-e641-4928-83be-5cfa9f893dbereponame:Repositório Institucional da UFSCARinstname:Universidade Federal de São Carlos (UFSCAR)instacron:UFSCARORIGINALDissertacao_Finalizada_2020_dez.pdfDissertacao_Finalizada_2020_dez.pdfDissertação de Mestradoapplication/pdf3996257https://repositorio.ufscar.br/bitstream/ufscar/13522/1/Dissertacao_Finalizada_2020_dez.pdf27d0d54b7ae61f0f3b9c29d468391a65MD51carta_comprovante_versão final_Thaís.pdfcarta_comprovante_versão final_Thaís.pdfCarta comprovanteapplication/pdf97624https://repositorio.ufscar.br/bitstream/ufscar/13522/2/carta_comprovante_vers%c3%a3o%20final_Tha%c3%ads.pdf02feaaf26cb28b72f1f704a684562be6MD52CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8811https://repositorio.ufscar.br/bitstream/ufscar/13522/3/license_rdfe39d27027a6cc9cb039ad269a5db8e34MD53TEXTDissertacao_Finalizada_2020_dez.pdf.txtDissertacao_Finalizada_2020_dez.pdf.txtExtracted texttext/plain167142https://repositorio.ufscar.br/bitstream/ufscar/13522/4/Dissertacao_Finalizada_2020_dez.pdf.txt5c5f12c4e86dcf811d7af4f801436a11MD54carta_comprovante_versão final_Thaís.pdf.txtcarta_comprovante_versão final_Thaís.pdf.txtExtracted texttext/plain1371https://repositorio.ufscar.br/bitstream/ufscar/13522/6/carta_comprovante_vers%c3%a3o%20final_Tha%c3%ads.pdf.txt9de4e176e388aee734b76f8ae036f50fMD56THUMBNAILDissertacao_Finalizada_2020_dez.pdf.jpgDissertacao_Finalizada_2020_dez.pdf.jpgIM Thumbnailimage/jpeg5406https://repositorio.ufscar.br/bitstream/ufscar/13522/5/Dissertacao_Finalizada_2020_dez.pdf.jpg3a2353f82a0b5a9ec8677fcf093c6e4aMD55carta_comprovante_versão final_Thaís.pdf.jpgcarta_comprovante_versão final_Thaís.pdf.jpgIM Thumbnailimage/jpeg13447https://repositorio.ufscar.br/bitstream/ufscar/13522/7/carta_comprovante_vers%c3%a3o%20final_Tha%c3%ads.pdf.jpgd273ef059df321b03c759d17a0558276MD57ufscar/135222023-09-18 18:32:04.513oai:repositorio.ufscar.br:ufscar/13522Repositório InstitucionalPUBhttps://repositorio.ufscar.br/oai/requestopendoar:43222023-09-18T18:32:04Repositório Institucional da UFSCAR - Universidade Federal de São Carlos (UFSCAR)false |
dc.title.por.fl_str_mv |
Obtenção de nanocelulose bacteriana de kombucha |
dc.title.alternative.por.fl_str_mv |
Obtaining of bacterial nanocellulose from kombucha |
title |
Obtenção de nanocelulose bacteriana de kombucha |
spellingShingle |
Obtenção de nanocelulose bacteriana de kombucha Góes, Thaís Soares de Celulose bacteriana Biopolímero Fontes renováveis Nanociência Nanoscience Renewable sources Bacterial cellulose Biopolymers CIENCIAS EXATAS E DA TERRA::QUIMICA |
title_short |
Obtenção de nanocelulose bacteriana de kombucha |
title_full |
Obtenção de nanocelulose bacteriana de kombucha |
title_fullStr |
Obtenção de nanocelulose bacteriana de kombucha |
title_full_unstemmed |
Obtenção de nanocelulose bacteriana de kombucha |
title_sort |
Obtenção de nanocelulose bacteriana de kombucha |
author |
Góes, Thaís Soares de |
author_facet |
Góes, Thaís Soares de |
author_role |
author |
dc.contributor.authorlattes.por.fl_str_mv |
http://lattes.cnpq.br/0357256587983464 |
dc.contributor.author.fl_str_mv |
Góes, Thaís Soares de |
dc.contributor.advisor1.fl_str_mv |
Silva, Adriana de Oliveira Delgado |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/2707979913713089 |
dc.contributor.advisor-co1.fl_str_mv |
Menezes, Aparecido Junior de |
dc.contributor.advisor-co1Lattes.fl_str_mv |
http://lattes.cnpq.br/0484426340349483 |
dc.contributor.authorID.fl_str_mv |
75c1544b-bd89-4d82-80df-8dd146193ee5 |
contributor_str_mv |
Silva, Adriana de Oliveira Delgado Menezes, Aparecido Junior de |
dc.subject.por.fl_str_mv |
Celulose bacteriana Biopolímero Fontes renováveis Nanociência Nanoscience Renewable sources Bacterial cellulose Biopolymers |
topic |
Celulose bacteriana Biopolímero Fontes renováveis Nanociência Nanoscience Renewable sources Bacterial cellulose Biopolymers CIENCIAS EXATAS E DA TERRA::QUIMICA |
dc.subject.cnpq.fl_str_mv |
CIENCIAS EXATAS E DA TERRA::QUIMICA |
description |
In the last years, sustainable materials that provide less environmental impact are gaining special attention. In this context, cellulose, a natural and abundant polymer from several renewable vegetable and microbial sources, is being highlighted in related literature. Bacterial cellulose (BC) from microbial sources presents several advantageous properties as lignin and hemicellulose absence, high resistance to traction, elasticity, durability, and a high degree of crystallinity. Also, BC is biodegradable, non-toxic, and biocompatible. A less explored source of BC includes Kombucha membranes (KM), a by-product of Kombucha's ancient beverage. KM are cellulosic pellicles derived from the fermentation of black or green tea broth. Thus, the main objective of this study was the production and characterization of the physical and chemical properties of BC obtained from Kombucha’s bacterial cellulose membranes (KBCM) based on the fermentation of green tea broth with added sucrose. We also aimed at the production of bacterial nanocellulose from Kombucha beverage (BNKB) via a hydrolytic reaction. KBCM was produced using a symbiotic consortium of bacteria and yeast during the fermentation of green tea broth and sucrose inoculated with an initial KBCM solution and fermented at 35°C for 21 days. After, the KBCM solution was purified using distilled water and sodium hydroxide (NaOH). BKNB were extracted via KBCM acid hydrolysis with 64% sulfuric acid at 50°C, and reaction time variation, followed by dialysis processes until reach a neutral pH value. KBCM were analyzed before and after the purification process using atomic force microscopy (AFM), scanning electron microscopy (SEM), energy-dispersive spectroscopy (EDS), Fourier transform infrared (FTIR) spectroscopy, and X-ray diffraction (XRD). Suspensions containing BNKB were characterized by AFM only. KBCM before and after purification were characterized by the techniques of atomic force microscopy (AFM), scanning electron microscopy (SEM), dispersive energy spectroscopy (EDS), infrared absorption spectroscopy with Fourier transform (FTIR) and Diffraction X-ray (XRD). BNKB suspensions were characterized only by AFM. AFM and SEM analyzes revealed the morphology of the BNKB structures in long fibrous chains, which were clustered and interlaced. The relative intensities by XRD varied after the purification process, indicating a possible change in crystallinity. The presence of carbon and oxygen elements in KBCM before and after purification were also confirmed by EDS. Thus, it is concluded that through the mentioned characteristics and the BNKB morphology, they offer resistance in their cellulosic chains during the acid attack, which can be observed by the AFM images, due to the presence of curvatures in the KC nanofibrils, which by time it leverages it as a promising source of raw material for the production of new sustainable materials. |
publishDate |
2020 |
dc.date.accessioned.fl_str_mv |
2020-12-04T16:37:51Z |
dc.date.available.fl_str_mv |
2020-12-04T16:37:51Z |
dc.date.issued.fl_str_mv |
2020-03-24 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
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masterThesis |
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publishedVersion |
dc.identifier.citation.fl_str_mv |
GÓES, Thaís Soares de. Obtenção de nanocelulose bacteriana de kombucha. 2020. Dissertação (Mestrado em Ciência dos Materiais) – Universidade Federal de São Carlos, Sorocaba, 2020. Disponível em: https://repositorio.ufscar.br/handle/ufscar/13522. |
dc.identifier.uri.fl_str_mv |
https://repositorio.ufscar.br/handle/ufscar/13522 |
identifier_str_mv |
GÓES, Thaís Soares de. Obtenção de nanocelulose bacteriana de kombucha. 2020. Dissertação (Mestrado em Ciência dos Materiais) – Universidade Federal de São Carlos, Sorocaba, 2020. Disponível em: https://repositorio.ufscar.br/handle/ufscar/13522. |
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https://repositorio.ufscar.br/handle/ufscar/13522 |
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por |
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600 600 |
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Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ info:eu-repo/semantics/openAccess |
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Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ |
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openAccess |
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Universidade Federal de São Carlos Câmpus Sorocaba |
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Programa de Pós-Graduação em Ciência dos Materiais - PPGCM-So |
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Universidade Federal de São Carlos Câmpus Sorocaba |
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