Bioactivity, chemical and microbial diversity in Alternanthera brasiliana : the influence of endophytes on antibiotic production

Detalhes bibliográficos
Autor(a) principal: Trapp, Marília Almeida
Data de Publicação: 2016
Tipo de documento: Tese
Idioma: eng
Título da fonte: Repositório Institucional da UFSCAR
Texto Completo: https://repositorio.ufscar.br/handle/ufscar/8846
Resumo: Alternanthera brasiliana is a plant widely used by Brazilian people as a folk medicine for the treatment of numerous diseases, especially due to its antibiotic properties. Using dereplication strategies, we identified antibiotic compounds as modified fatty acids, which are closely related to oxylipins. The biosynthesis of these compounds could be related with the presence of endophytic bacteria. Thus, we established axenic cultures of A. brasiliana and developed methods for detection and quantification of bacterial cells in plant tissues. We also performed initial re-inoculation experiments with endophytic bacteria isolated from A. brasiliana. In addition, method for quantification of phytohormones and oxylipins in plant tissues were developed. The results reinforced the correlation between both the amount of bacteria and oxylipins in A. brasiliana tissues.
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spelling Trapp, Marília AlmeidaRodrigues Filho, Edsonhttp://lattes.cnpq.br/3667941735597178http://lattes.cnpq.br/40768943762187777ded997f-1a92-4b29-8952-d6e773aa2fc82017-06-12T20:03:06Z2017-06-12T20:03:06Z2016-02-25TRAPP, Marília Almeida. Bioactivity, chemical and microbial diversity in Alternanthera brasiliana : the influence of endophytes on antibiotic production. 2016. Tese (Doutorado em Química) – Universidade Federal de São Carlos, São Carlos, 2016. Disponível em: https://repositorio.ufscar.br/handle/ufscar/8846.https://repositorio.ufscar.br/handle/ufscar/8846Alternanthera brasiliana is a plant widely used by Brazilian people as a folk medicine for the treatment of numerous diseases, especially due to its antibiotic properties. Using dereplication strategies, we identified antibiotic compounds as modified fatty acids, which are closely related to oxylipins. The biosynthesis of these compounds could be related with the presence of endophytic bacteria. Thus, we established axenic cultures of A. brasiliana and developed methods for detection and quantification of bacterial cells in plant tissues. We also performed initial re-inoculation experiments with endophytic bacteria isolated from A. brasiliana. In addition, method for quantification of phytohormones and oxylipins in plant tissues were developed. The results reinforced the correlation between both the amount of bacteria and oxylipins in A. brasiliana tissues.Alternanthera brasiliana é uma planta amplamente utilizada na medicina popular brasileira para o tratamento de diversas doenças, principalmente devido a suas propriedades antibióticas. Durante este trabalho, utilizou-se estratégias de desreplicação a fim de se identificar os compostos antibióticos presentes nesta planta. Esses compostos foram identificados como ácidos graxos oxidados pertencentes a classe das oxilipinas. A biossíntese destes compostos esta comumente relacionada a situações de estresse biótico e abiótico em plantas. Dessa forma, nos estabelecemos métodos para a detecção e quantificação de células bacterianas em tecidos vegetais. Também foram realizados experimentos iniciais de re-inoculação de bactérias endofíticas isoladas de A. brasiliana. Além disso, foram desenvolvidos métodos para a quantificação de fitohormônios e oxilipinas em tecidos vegetais. Os resultados obtidos até o momento ressaltam a possibilidade de bactérias endofíticas influenciarem a produção de oxilipinas (compostos antibióticos) na planta A. brasiliana..Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)FAPESP: 2007/03771-7FAPESP: 2008/07494-0FAPESP: 2010/17925-9FAPESP: 2012/20932-2engUniversidade Federal de São CarlosCâmpus São CarlosPrograma de Pós-Graduação em Química - PPGQUFSCarAlternanthera brasilianaDereplicationAntibioticChemical ecologyPlant-bacteriaCIENCIAS EXATAS E DA TERRA::QUIMICABioactivity, chemical and microbial diversity in Alternanthera brasiliana : the influence of endophytes on antibiotic productionBioatividade, diversidade química e microbiana associadas à Alternanthera brasiliana : a influência de micro-organismos endofíticos na produção de compostos antibióticosinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisOnline60038736a86-d071-4ee1-8969-144f8d20b7ccinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFSCARinstname:Universidade Federal de São Carlos (UFSCAR)instacron:UFSCARORIGINALTeseMAT.pdfTeseMAT.pdfapplication/pdf10785085https://repositorio.ufscar.br/bitstream/ufscar/8846/1/TeseMAT.pdf69ccc2d94ef0cf944d85e6cf91c78d59MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81957https://repositorio.ufscar.br/bitstream/ufscar/8846/2/license.txtae0398b6f8b235e40ad82cba6c50031dMD52TEXTTeseMAT.pdf.txtTeseMAT.pdf.txtExtracted texttext/plain272383https://repositorio.ufscar.br/bitstream/ufscar/8846/3/TeseMAT.pdf.txtfb4583db74554f855d1dcd0962039092MD53THUMBNAILTeseMAT.pdf.jpgTeseMAT.pdf.jpgIM Thumbnailimage/jpeg8999https://repositorio.ufscar.br/bitstream/ufscar/8846/4/TeseMAT.pdf.jpgc3760313773e59cc67a34fcdb8c55992MD54ufscar/88462023-09-18 18:31:24.728oai:repositorio.ufscar.br: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Repositório InstitucionalPUBhttps://repositorio.ufscar.br/oai/requestopendoar:43222023-09-18T18:31:24Repositório Institucional da UFSCAR - Universidade Federal de São Carlos (UFSCAR)false
dc.title.eng.fl_str_mv Bioactivity, chemical and microbial diversity in Alternanthera brasiliana : the influence of endophytes on antibiotic production
dc.title.alternative.por.fl_str_mv Bioatividade, diversidade química e microbiana associadas à Alternanthera brasiliana : a influência de micro-organismos endofíticos na produção de compostos antibióticos
title Bioactivity, chemical and microbial diversity in Alternanthera brasiliana : the influence of endophytes on antibiotic production
spellingShingle Bioactivity, chemical and microbial diversity in Alternanthera brasiliana : the influence of endophytes on antibiotic production
Trapp, Marília Almeida
Alternanthera brasiliana
Dereplication
Antibiotic
Chemical ecology
Plant-bacteria
CIENCIAS EXATAS E DA TERRA::QUIMICA
title_short Bioactivity, chemical and microbial diversity in Alternanthera brasiliana : the influence of endophytes on antibiotic production
title_full Bioactivity, chemical and microbial diversity in Alternanthera brasiliana : the influence of endophytes on antibiotic production
title_fullStr Bioactivity, chemical and microbial diversity in Alternanthera brasiliana : the influence of endophytes on antibiotic production
title_full_unstemmed Bioactivity, chemical and microbial diversity in Alternanthera brasiliana : the influence of endophytes on antibiotic production
title_sort Bioactivity, chemical and microbial diversity in Alternanthera brasiliana : the influence of endophytes on antibiotic production
author Trapp, Marília Almeida
author_facet Trapp, Marília Almeida
author_role author
dc.contributor.authorlattes.por.fl_str_mv http://lattes.cnpq.br/4076894376218777
dc.contributor.author.fl_str_mv Trapp, Marília Almeida
dc.contributor.advisor1.fl_str_mv Rodrigues Filho, Edson
dc.contributor.advisor1Lattes.fl_str_mv http://lattes.cnpq.br/3667941735597178
dc.contributor.authorID.fl_str_mv 7ded997f-1a92-4b29-8952-d6e773aa2fc8
contributor_str_mv Rodrigues Filho, Edson
dc.subject.lat.fl_str_mv Alternanthera brasiliana
topic Alternanthera brasiliana
Dereplication
Antibiotic
Chemical ecology
Plant-bacteria
CIENCIAS EXATAS E DA TERRA::QUIMICA
dc.subject.eng.fl_str_mv Dereplication
Antibiotic
Chemical ecology
Plant-bacteria
dc.subject.cnpq.fl_str_mv CIENCIAS EXATAS E DA TERRA::QUIMICA
description Alternanthera brasiliana is a plant widely used by Brazilian people as a folk medicine for the treatment of numerous diseases, especially due to its antibiotic properties. Using dereplication strategies, we identified antibiotic compounds as modified fatty acids, which are closely related to oxylipins. The biosynthesis of these compounds could be related with the presence of endophytic bacteria. Thus, we established axenic cultures of A. brasiliana and developed methods for detection and quantification of bacterial cells in plant tissues. We also performed initial re-inoculation experiments with endophytic bacteria isolated from A. brasiliana. In addition, method for quantification of phytohormones and oxylipins in plant tissues were developed. The results reinforced the correlation between both the amount of bacteria and oxylipins in A. brasiliana tissues.
publishDate 2016
dc.date.issued.fl_str_mv 2016-02-25
dc.date.accessioned.fl_str_mv 2017-06-12T20:03:06Z
dc.date.available.fl_str_mv 2017-06-12T20:03:06Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/doctoralThesis
format doctoralThesis
status_str publishedVersion
dc.identifier.citation.fl_str_mv TRAPP, Marília Almeida. Bioactivity, chemical and microbial diversity in Alternanthera brasiliana : the influence of endophytes on antibiotic production. 2016. Tese (Doutorado em Química) – Universidade Federal de São Carlos, São Carlos, 2016. Disponível em: https://repositorio.ufscar.br/handle/ufscar/8846.
dc.identifier.uri.fl_str_mv https://repositorio.ufscar.br/handle/ufscar/8846
identifier_str_mv TRAPP, Marília Almeida. Bioactivity, chemical and microbial diversity in Alternanthera brasiliana : the influence of endophytes on antibiotic production. 2016. Tese (Doutorado em Química) – Universidade Federal de São Carlos, São Carlos, 2016. Disponível em: https://repositorio.ufscar.br/handle/ufscar/8846.
url https://repositorio.ufscar.br/handle/ufscar/8846
dc.language.iso.fl_str_mv eng
language eng
dc.relation.confidence.fl_str_mv 600
dc.relation.authority.fl_str_mv 38736a86-d071-4ee1-8969-144f8d20b7cc
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Universidade Federal de São Carlos
Câmpus São Carlos
dc.publisher.program.fl_str_mv Programa de Pós-Graduação em Química - PPGQ
dc.publisher.initials.fl_str_mv UFSCar
publisher.none.fl_str_mv Universidade Federal de São Carlos
Câmpus São Carlos
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFSCAR
instname:Universidade Federal de São Carlos (UFSCAR)
instacron:UFSCAR
instname_str Universidade Federal de São Carlos (UFSCAR)
instacron_str UFSCAR
institution UFSCAR
reponame_str Repositório Institucional da UFSCAR
collection Repositório Institucional da UFSCAR
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