Análise genotóxica do Fipronil® no cérebro de abelhas da espécie Bombus atratus (HYMENOPTERA, BOMBINI)

Detalhes bibliográficos
Autor(a) principal: Provase, Michele
Data de Publicação: 2022
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Repositório Institucional da UFSCAR
Texto Completo: https://repositorio.ufscar.br/handle/ufscar/15666
Resumo: Fipronil® is a neurotoxic insecticide, effective for control of crop pests. However, there are no data about possible genotoxic effect of sublethal concentrations in the brain of pollinator bees. Therefore, this study aimed sought to analyze the genotoxicity of fipronil® at two concentrations estimated in the field in the brain of foraging workers of Bombus atratus through microelectrophoresis. Workers of B. atratus were collected in the field and transferred to laboratory where they were kept isolately at 26 ºC and 70% relative humidity for 96 hours. Exposure was performed by offering a solution contaminated with fipronil at 2.5 ppb and 3.5 ppb and water as control. Sucrose syrup at 70% was offered to all groups as food ad libitum. After exposure, the workers were crio-anesthezied at 4 ºC and dissected, the removed brain was macerated and centrifuged to isolate the nuclei. The prepared samples were submitted to the Comet Assay technique. Comets were analyzed using an optical microscope, and by the parameters: Damage Index (ID), Damage Frequency (FD%) and Total Damage (DT). Tukey's statistical test was also applied (p<0.05). The results showed that the mean values obtained for ID and FD% showed notable differences between the experimental groups in relation to the control group. However, when analyzing DT through the Tukey test, it was observed that the concentration of 2.5 ppb did not cause significant damage to the DNA of the neural cells of B. atratus (p=0.4434), as observed in the concentration of 3.5 ppb, that induced significant genotoxic effects in the brain of these bees (p=0.0215). Thus, it is concluded that the concentration found in fields of 3.5 ppb is genotoxic to the brain of B. atratus workers.
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spelling Provase, MicheleAbdalla, Fábio Camargohttp://lattes.cnpq.br/7770803221041927http://lattes.cnpq.br/7262712256652151c0247a8e-a6f2-43db-a19a-de301017507c2022-03-07T17:29:21Z2022-03-07T17:29:21Z2022-02-22PROVASE, Michele. Análise genotóxica do Fipronil® no cérebro de abelhas da espécie Bombus atratus (HYMENOPTERA, BOMBINI). 2022. Dissertação (Mestrado em Biotecnologia e Monitoramento Ambiental) – Universidade Federal de São Carlos, Sorocaba, 2022. Disponível em: https://repositorio.ufscar.br/handle/ufscar/15666.https://repositorio.ufscar.br/handle/ufscar/15666Fipronil® is a neurotoxic insecticide, effective for control of crop pests. However, there are no data about possible genotoxic effect of sublethal concentrations in the brain of pollinator bees. Therefore, this study aimed sought to analyze the genotoxicity of fipronil® at two concentrations estimated in the field in the brain of foraging workers of Bombus atratus through microelectrophoresis. Workers of B. atratus were collected in the field and transferred to laboratory where they were kept isolately at 26 ºC and 70% relative humidity for 96 hours. Exposure was performed by offering a solution contaminated with fipronil at 2.5 ppb and 3.5 ppb and water as control. Sucrose syrup at 70% was offered to all groups as food ad libitum. After exposure, the workers were crio-anesthezied at 4 ºC and dissected, the removed brain was macerated and centrifuged to isolate the nuclei. The prepared samples were submitted to the Comet Assay technique. Comets were analyzed using an optical microscope, and by the parameters: Damage Index (ID), Damage Frequency (FD%) and Total Damage (DT). Tukey's statistical test was also applied (p<0.05). The results showed that the mean values obtained for ID and FD% showed notable differences between the experimental groups in relation to the control group. However, when analyzing DT through the Tukey test, it was observed that the concentration of 2.5 ppb did not cause significant damage to the DNA of the neural cells of B. atratus (p=0.4434), as observed in the concentration of 3.5 ppb, that induced significant genotoxic effects in the brain of these bees (p=0.0215). Thus, it is concluded that the concentration found in fields of 3.5 ppb is genotoxic to the brain of B. atratus workers.O fipronil® é um inseticida de ação neurotóxica, efetivo no combate às pragas. Atualmente não há estudos sobre os seus efeitos genotóxico no cérebro de abelhas. Em razão disso, o presente estudo buscou verificar a genotoxicidade do fipronil® às duas concentrações estimadas em campo no cérebro de operárias de Bombus atratus através de microeletroforese. Operárias de B. atratus foram coletadas em campo e transferidas para o laboratório onde foram mantidas isoladamente em estufa B.O.D. (26 °C, 70% umidade relativa) por 96 horas. A exposição foi realizada oferecendo uma solução contaminada com fipronil à 2,5 ppb e 3,5 ppb. O grupo controle mantido em mesma condição foi ofertada água filtrada. Para todos os grupos foram ofertada sacarose 70% ad libitum como alimento. Após exposição, as abelhas foram crio-anestesiadas e dissecadas, o cérebro retirado foi macerado e centrifugado para isolamento dos núcleos. As amostras preparadas foram submetidas à técnica Ensaio Cometa. Os cometas foram analisados através de microscópio ótico, e pelos parâmetros: Índice de Danos (ID), Frequência de Dano (FD%) e Dano total (DT). Aplicou-se também teste estatístico de Tukey (p<0,05). Os resultados mostraram que os valores médios obtidos para ID e FD% apresentaram diferenças notáveis entre os grupos experimentais em relação ao grupo controle. Entretanto, ao analisar DT através do teste de Tukey, observou-se que a concentração 2,5 ppb não causou danos significativos ao DNA nas células neurais de B. atratus (p=0,4434), como observado na concentração de 3,5 ppb que induziu efeitos genotóxicos significativos ao cérebro dessas abelhas (p=0,0215). Assim, conclui-se que a concentração encontrada em campos de 3,5 ppb é genotóxica ao cérebro de operárias de B. atratus.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)O presente trabalho foi realizado com apoio da Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - Brasil (CAPES) - Código de Financiamento 001, processo nº 88887.492976/2020-00porUniversidade Federal de São CarlosCâmpus SorocabaPrograma de Pós-Graduação em Biotecnologia e Monitoramento Ambiental - PPGBMA-SoUFSCarAttribution-NonCommercial-NoDerivs 3.0 Brazilhttp://creativecommons.org/licenses/by-nc-nd/3.0/br/info:eu-repo/semantics/openAccessEnsaio cometaBombus atratusFipronilGenotoxicidadecomet assayGenotoxicitCIENCIAS BIOLOGICAS::BIOLOGIA GERALAnálise genotóxica do Fipronil® no cérebro de abelhas da espécie Bombus atratus (HYMENOPTERA, BOMBINI)Genotoxicity of Fipronil® in the brain of bumblebee Bombus atratus (Hymenopter: Bombini)info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis600600c4f0703e-797c-491e-889d-533cc39b3edbreponame:Repositório Institucional da UFSCARinstname:Universidade Federal de São Carlos (UFSCAR)instacron:UFSCARORIGINALDissertação (2022).pdfDissertação (2022).pdfDissertaçãoapplication/pdf2150380https://repositorio.ufscar.br/bitstream/ufscar/15666/1/Disserta%c3%a7%c3%a3o%20%282022%29.pdf9c89525909bcf27044686d07a5cfc26bMD51Carta comprovante da versão final de dissertação.pdfCarta comprovante da versão final de dissertação.pdfapplication/pdf203291https://repositorio.ufscar.br/bitstream/ufscar/15666/2/Carta%20comprovante%20da%20vers%c3%a3o%20final%20de%20disserta%c3%a7%c3%a3o.pdf4a35f74d790531326c8cd42b1b21cf34MD52CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8811https://repositorio.ufscar.br/bitstream/ufscar/15666/3/license_rdfe39d27027a6cc9cb039ad269a5db8e34MD53TEXTDissertação (2022).pdf.txtDissertação (2022).pdf.txtExtracted texttext/plain131961https://repositorio.ufscar.br/bitstream/ufscar/15666/4/Disserta%c3%a7%c3%a3o%20%282022%29.pdf.txt53f1b56ee4756bed6497693871866f90MD54Carta comprovante da versão final de dissertação.pdf.txtCarta comprovante da versão final de dissertação.pdf.txtExtracted texttext/plain1528https://repositorio.ufscar.br/bitstream/ufscar/15666/6/Carta%20comprovante%20da%20vers%c3%a3o%20final%20de%20disserta%c3%a7%c3%a3o.pdf.txta7245715647dfb3ae86097a440c3d26bMD56THUMBNAILDissertação (2022).pdf.jpgDissertação (2022).pdf.jpgIM Thumbnailimage/jpeg6284https://repositorio.ufscar.br/bitstream/ufscar/15666/5/Disserta%c3%a7%c3%a3o%20%282022%29.pdf.jpgc5784b054fefa12c320a3c54a2c25218MD55Carta comprovante da versão final de dissertação.pdf.jpgCarta comprovante da versão final de dissertação.pdf.jpgIM Thumbnailimage/jpeg14191https://repositorio.ufscar.br/bitstream/ufscar/15666/7/Carta%20comprovante%20da%20vers%c3%a3o%20final%20de%20disserta%c3%a7%c3%a3o.pdf.jpg2f838a6e12805b030d382096923027c5MD57ufscar/156662023-09-18 18:32:28.037oai:repositorio.ufscar.br:ufscar/15666Repositório InstitucionalPUBhttps://repositorio.ufscar.br/oai/requestopendoar:43222023-09-18T18:32:28Repositório Institucional da UFSCAR - Universidade Federal de São Carlos (UFSCAR)false
dc.title.por.fl_str_mv Análise genotóxica do Fipronil® no cérebro de abelhas da espécie Bombus atratus (HYMENOPTERA, BOMBINI)
dc.title.alternative.eng.fl_str_mv Genotoxicity of Fipronil® in the brain of bumblebee Bombus atratus (Hymenopter: Bombini)
title Análise genotóxica do Fipronil® no cérebro de abelhas da espécie Bombus atratus (HYMENOPTERA, BOMBINI)
spellingShingle Análise genotóxica do Fipronil® no cérebro de abelhas da espécie Bombus atratus (HYMENOPTERA, BOMBINI)
Provase, Michele
Ensaio cometa
Bombus atratus
Fipronil
Genotoxicidade
comet assay
Genotoxicit
CIENCIAS BIOLOGICAS::BIOLOGIA GERAL
title_short Análise genotóxica do Fipronil® no cérebro de abelhas da espécie Bombus atratus (HYMENOPTERA, BOMBINI)
title_full Análise genotóxica do Fipronil® no cérebro de abelhas da espécie Bombus atratus (HYMENOPTERA, BOMBINI)
title_fullStr Análise genotóxica do Fipronil® no cérebro de abelhas da espécie Bombus atratus (HYMENOPTERA, BOMBINI)
title_full_unstemmed Análise genotóxica do Fipronil® no cérebro de abelhas da espécie Bombus atratus (HYMENOPTERA, BOMBINI)
title_sort Análise genotóxica do Fipronil® no cérebro de abelhas da espécie Bombus atratus (HYMENOPTERA, BOMBINI)
author Provase, Michele
author_facet Provase, Michele
author_role author
dc.contributor.authorlattes.por.fl_str_mv http://lattes.cnpq.br/7262712256652151
dc.contributor.author.fl_str_mv Provase, Michele
dc.contributor.advisor1.fl_str_mv Abdalla, Fábio Camargo
dc.contributor.advisor1Lattes.fl_str_mv http://lattes.cnpq.br/7770803221041927
dc.contributor.authorID.fl_str_mv c0247a8e-a6f2-43db-a19a-de301017507c
contributor_str_mv Abdalla, Fábio Camargo
dc.subject.por.fl_str_mv Ensaio cometa
Bombus atratus
Fipronil
Genotoxicidade
topic Ensaio cometa
Bombus atratus
Fipronil
Genotoxicidade
comet assay
Genotoxicit
CIENCIAS BIOLOGICAS::BIOLOGIA GERAL
dc.subject.eng.fl_str_mv comet assay
Genotoxicit
dc.subject.cnpq.fl_str_mv CIENCIAS BIOLOGICAS::BIOLOGIA GERAL
description Fipronil® is a neurotoxic insecticide, effective for control of crop pests. However, there are no data about possible genotoxic effect of sublethal concentrations in the brain of pollinator bees. Therefore, this study aimed sought to analyze the genotoxicity of fipronil® at two concentrations estimated in the field in the brain of foraging workers of Bombus atratus through microelectrophoresis. Workers of B. atratus were collected in the field and transferred to laboratory where they were kept isolately at 26 ºC and 70% relative humidity for 96 hours. Exposure was performed by offering a solution contaminated with fipronil at 2.5 ppb and 3.5 ppb and water as control. Sucrose syrup at 70% was offered to all groups as food ad libitum. After exposure, the workers were crio-anesthezied at 4 ºC and dissected, the removed brain was macerated and centrifuged to isolate the nuclei. The prepared samples were submitted to the Comet Assay technique. Comets were analyzed using an optical microscope, and by the parameters: Damage Index (ID), Damage Frequency (FD%) and Total Damage (DT). Tukey's statistical test was also applied (p<0.05). The results showed that the mean values obtained for ID and FD% showed notable differences between the experimental groups in relation to the control group. However, when analyzing DT through the Tukey test, it was observed that the concentration of 2.5 ppb did not cause significant damage to the DNA of the neural cells of B. atratus (p=0.4434), as observed in the concentration of 3.5 ppb, that induced significant genotoxic effects in the brain of these bees (p=0.0215). Thus, it is concluded that the concentration found in fields of 3.5 ppb is genotoxic to the brain of B. atratus workers.
publishDate 2022
dc.date.accessioned.fl_str_mv 2022-03-07T17:29:21Z
dc.date.available.fl_str_mv 2022-03-07T17:29:21Z
dc.date.issued.fl_str_mv 2022-02-22
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dc.identifier.citation.fl_str_mv PROVASE, Michele. Análise genotóxica do Fipronil® no cérebro de abelhas da espécie Bombus atratus (HYMENOPTERA, BOMBINI). 2022. Dissertação (Mestrado em Biotecnologia e Monitoramento Ambiental) – Universidade Federal de São Carlos, Sorocaba, 2022. Disponível em: https://repositorio.ufscar.br/handle/ufscar/15666.
dc.identifier.uri.fl_str_mv https://repositorio.ufscar.br/handle/ufscar/15666
identifier_str_mv PROVASE, Michele. Análise genotóxica do Fipronil® no cérebro de abelhas da espécie Bombus atratus (HYMENOPTERA, BOMBINI). 2022. Dissertação (Mestrado em Biotecnologia e Monitoramento Ambiental) – Universidade Federal de São Carlos, Sorocaba, 2022. Disponível em: https://repositorio.ufscar.br/handle/ufscar/15666.
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