Toxicidade de nanopartículas de óxido de ferro (Fe3O4) para o cladócero tropical Ceriodaphnia silvestrii
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFSCAR |
Texto Completo: | https://repositorio.ufscar.br/handle/ufscar/9045 |
Resumo: | Nanoparticles (NPs) have been produced on a large scale worldwide for various consumer purposes such as the production of cosmetics, sunscreen, biosensors, human prosthetics and cancer therapy. However, due to its large production, NPs can have water bodies as final destination, where current studies addressing these compounds are not sufficient to estimate the threat that these substances could cause to aquatic organisms. Chronic studies with nano-Fe3O4 are scarce and, to the best of our knowledge, inexistent regarding tropical zooplankton species, although chronic studies are of great importance to predict the effects of the substances because they analyses great part of this organisms life cycle. At present study, we investigated nanoparticles of Fe3O4, to the tropical cladoceran Ceriodaphnia silvestrii using acute toxicity tests (0.00; 0.01; 0.10; 1.00; 10.00 and 100.00 mg L-1) during 48h, and chronic toxicity tests (0.00; 3.125; 6.25; 12.50; 25.00 and 50.00 mg L-1) during 14 days. Characterization of NPs in the exposure medium revealed that they experienced agglomeration and aggregation on a micrometer scale. Results showed non-toxicity of nano-Fe3O4 after 48h of acute exposure (EC5048h > 100.00 mg L-1). ). In chronic tests, treatment of 50 mg L-1 caused significant inhibition of growth and reproduction, affecting the maximum length (inhibited 12.71%), accumulated number of eggs (reduction of 51.99%) and neonates (decrease of 61.37%) per female (Dunnett’s test, p < 0.05). We concluded that nano-Fe3O4, in the 14th day, was toxic to C. silvestrii only at the highest concentration tested (50.00 mg L-1) during this chronic exposure.. The use of nano-Fe3O4 in aquatic environments could be considered safe to this species in concentrations up to 25.00 mg L-1, according to the parameters evaluated. |
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Gebara, Renan CastelhanoMelão, Maria da Graça Gamahttp://lattes.cnpq.br/8342306603661310Souza, Jaqueline Pérola dehttp://lattes.cnpq.br/2320506014760329http://lattes.cnpq.br/75528263884787845ae80bcf-9888-4374-8b17-a7303ac7f2182017-08-22T14:00:38Z2017-08-22T14:00:38Z2017-03-02GEBARA, Renan Castelhano. Toxicidade de nanopartículas de óxido de ferro (Fe3O4) para o cladócero tropical Ceriodaphnia silvestrii. 2017. Dissertação (Mestrado em Ecologia e Recursos Naturais) – Universidade Federal de São Carlos, São Carlos, 2017. Disponível em: https://repositorio.ufscar.br/handle/ufscar/9045.https://repositorio.ufscar.br/handle/ufscar/9045Nanoparticles (NPs) have been produced on a large scale worldwide for various consumer purposes such as the production of cosmetics, sunscreen, biosensors, human prosthetics and cancer therapy. However, due to its large production, NPs can have water bodies as final destination, where current studies addressing these compounds are not sufficient to estimate the threat that these substances could cause to aquatic organisms. Chronic studies with nano-Fe3O4 are scarce and, to the best of our knowledge, inexistent regarding tropical zooplankton species, although chronic studies are of great importance to predict the effects of the substances because they analyses great part of this organisms life cycle. At present study, we investigated nanoparticles of Fe3O4, to the tropical cladoceran Ceriodaphnia silvestrii using acute toxicity tests (0.00; 0.01; 0.10; 1.00; 10.00 and 100.00 mg L-1) during 48h, and chronic toxicity tests (0.00; 3.125; 6.25; 12.50; 25.00 and 50.00 mg L-1) during 14 days. Characterization of NPs in the exposure medium revealed that they experienced agglomeration and aggregation on a micrometer scale. Results showed non-toxicity of nano-Fe3O4 after 48h of acute exposure (EC5048h > 100.00 mg L-1). ). In chronic tests, treatment of 50 mg L-1 caused significant inhibition of growth and reproduction, affecting the maximum length (inhibited 12.71%), accumulated number of eggs (reduction of 51.99%) and neonates (decrease of 61.37%) per female (Dunnett’s test, p < 0.05). We concluded that nano-Fe3O4, in the 14th day, was toxic to C. silvestrii only at the highest concentration tested (50.00 mg L-1) during this chronic exposure.. The use of nano-Fe3O4 in aquatic environments could be considered safe to this species in concentrations up to 25.00 mg L-1, according to the parameters evaluated.As nanopartículas (NPs) têm sido amplamente produzidas em larga escala para vários propósitos tais como: produção de cosméticos, protetores solares, biosensores, próteses humanas e tratamento do câncer. No entanto, devido à ampla produção, eventualmente as NPs podem encontrar seu destino final nos corpos d’água, onde os estudos relativos à presença dessas substâncias muitas vezes não são suficientes para estimar os efeitos que as mesmas poderiam causar nos organismos aquáticos. Estudos de toxicidade crônica com nanopartículas de óxido de ferro (nano-Fe3O4) são escassos e, até onde sabemos, inexistentes para cladóceros tropicais, embora avaliações crônicas sejam de grande importância para predição dos efeitos de substâncias, pois abrangem grande parte do ciclo de vida dos organismos. No presente estudo, foram estudadas nanopartículas de Fe3O4 para cladócero neotropical Ceriodaphnia silvestrii, por meio de testes de toxicidade aguda (0,00; 0,01; 0,10; 1,00; 10,00 e 100,00 mg L-1), durante 48 horas, e crônica (0,00; 3,125; 6,25; 12,50; 25,00 e 50,00 mg L-1), durante 14 dias. A caracterização das NPs nos meios de exposição revelou que elas sofreram aglomeração e agregação em escalas micrométricas. Os resultados obtidos apontaram ausência de toxicidade aguda para as nano-Fe3O4 (CE(I)5048h > 100,00 mg L-1). Nos testes de toxicidade crônica, no tratamento de 50,00 mg L-1, houve inibição significativa no crescimento e reprodução, afetando o comprimento máximo (inibição de 12,71%) e o número acumulado de ovos (diminuição de 51,99%) e de neonatas (diminuição de 61,37%) produzidos por fêmea (teste de Dunnett p < 0.05). Concluiu-se que as nano-Fe3O4 apresentaram efeitos crônicos, no 14º dia, para o cladócero C. silvestrii somente na maior concentração avaliada (50,00 mg L-1). O uso de nano-Fe3O4 em ambientes aquáticos pode ser considerado seguro para esta espécie de cladócero tropical, com base nos parâmetros avaliados, até concentrações de 25,00 mg L-1.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)CNPq: 132379/2015-5FAPESP: 2014/14139-3FAPESP: 2016/00753-7porUniversidade Federal de São CarlosCâmpus São CarlosPrograma de Pós-Graduação em Ecologia e Recursos Naturais - PPGERNUFSCarEcotoxicologiaNanotoxicologiaNanopartículas magnéticasMagnetitaMicrocrustáceosEspécies neotropicaisEcotoxicologyNanotoxicologyMagnetic nanoparticlesMagnetiteMicrocrustaceansNeotropical speciesCIENCIAS BIOLOGICAS::ECOLOGIAToxicidade de nanopartículas de óxido de ferro (Fe3O4) para o cladócero tropical Ceriodaphnia silvestriiinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisOnline600600dc176969-1756-4354-8ff1-5242c10033aeinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFSCARinstname:Universidade Federal de São Carlos (UFSCAR)instacron:UFSCARORIGINALDissRCG.pdfDissRCG.pdfapplication/pdf1738048https://repositorio.ufscar.br/bitstream/ufscar/9045/1/DissRCG.pdf5fe499e8468dd911a3576a5aaf9afff3MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81957https://repositorio.ufscar.br/bitstream/ufscar/9045/2/license.txtae0398b6f8b235e40ad82cba6c50031dMD52TEXTDissRCG.pdf.txtDissRCG.pdf.txtExtracted texttext/plain104331https://repositorio.ufscar.br/bitstream/ufscar/9045/3/DissRCG.pdf.txt0ecbc757c90d7e8a6763f8818d9d4f97MD53THUMBNAILDissRCG.pdf.jpgDissRCG.pdf.jpgIM Thumbnailimage/jpeg7165https://repositorio.ufscar.br/bitstream/ufscar/9045/4/DissRCG.pdf.jpgfdfb645d690796e2d5f3986e97820bb9MD54ufscar/90452023-09-18 18:31:22.158oai:repositorio.ufscar.br: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Repositório InstitucionalPUBhttps://repositorio.ufscar.br/oai/requestopendoar:43222023-09-18T18:31:22Repositório Institucional da UFSCAR - Universidade Federal de São Carlos (UFSCAR)false |
dc.title.por.fl_str_mv |
Toxicidade de nanopartículas de óxido de ferro (Fe3O4) para o cladócero tropical Ceriodaphnia silvestrii |
title |
Toxicidade de nanopartículas de óxido de ferro (Fe3O4) para o cladócero tropical Ceriodaphnia silvestrii |
spellingShingle |
Toxicidade de nanopartículas de óxido de ferro (Fe3O4) para o cladócero tropical Ceriodaphnia silvestrii Gebara, Renan Castelhano Ecotoxicologia Nanotoxicologia Nanopartículas magnéticas Magnetita Microcrustáceos Espécies neotropicais Ecotoxicology Nanotoxicology Magnetic nanoparticles Magnetite Microcrustaceans Neotropical species CIENCIAS BIOLOGICAS::ECOLOGIA |
title_short |
Toxicidade de nanopartículas de óxido de ferro (Fe3O4) para o cladócero tropical Ceriodaphnia silvestrii |
title_full |
Toxicidade de nanopartículas de óxido de ferro (Fe3O4) para o cladócero tropical Ceriodaphnia silvestrii |
title_fullStr |
Toxicidade de nanopartículas de óxido de ferro (Fe3O4) para o cladócero tropical Ceriodaphnia silvestrii |
title_full_unstemmed |
Toxicidade de nanopartículas de óxido de ferro (Fe3O4) para o cladócero tropical Ceriodaphnia silvestrii |
title_sort |
Toxicidade de nanopartículas de óxido de ferro (Fe3O4) para o cladócero tropical Ceriodaphnia silvestrii |
author |
Gebara, Renan Castelhano |
author_facet |
Gebara, Renan Castelhano |
author_role |
author |
dc.contributor.authorlattes.por.fl_str_mv |
http://lattes.cnpq.br/7552826388478784 |
dc.contributor.author.fl_str_mv |
Gebara, Renan Castelhano |
dc.contributor.advisor1.fl_str_mv |
Melão, Maria da Graça Gama |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/8342306603661310 |
dc.contributor.advisor-co1.fl_str_mv |
Souza, Jaqueline Pérola de |
dc.contributor.advisor-co1Lattes.fl_str_mv |
http://lattes.cnpq.br/2320506014760329 |
dc.contributor.authorID.fl_str_mv |
5ae80bcf-9888-4374-8b17-a7303ac7f218 |
contributor_str_mv |
Melão, Maria da Graça Gama Souza, Jaqueline Pérola de |
dc.subject.por.fl_str_mv |
Ecotoxicologia Nanotoxicologia Nanopartículas magnéticas Magnetita Microcrustáceos Espécies neotropicais |
topic |
Ecotoxicologia Nanotoxicologia Nanopartículas magnéticas Magnetita Microcrustáceos Espécies neotropicais Ecotoxicology Nanotoxicology Magnetic nanoparticles Magnetite Microcrustaceans Neotropical species CIENCIAS BIOLOGICAS::ECOLOGIA |
dc.subject.eng.fl_str_mv |
Ecotoxicology Nanotoxicology Magnetic nanoparticles Magnetite Microcrustaceans Neotropical species |
dc.subject.cnpq.fl_str_mv |
CIENCIAS BIOLOGICAS::ECOLOGIA |
description |
Nanoparticles (NPs) have been produced on a large scale worldwide for various consumer purposes such as the production of cosmetics, sunscreen, biosensors, human prosthetics and cancer therapy. However, due to its large production, NPs can have water bodies as final destination, where current studies addressing these compounds are not sufficient to estimate the threat that these substances could cause to aquatic organisms. Chronic studies with nano-Fe3O4 are scarce and, to the best of our knowledge, inexistent regarding tropical zooplankton species, although chronic studies are of great importance to predict the effects of the substances because they analyses great part of this organisms life cycle. At present study, we investigated nanoparticles of Fe3O4, to the tropical cladoceran Ceriodaphnia silvestrii using acute toxicity tests (0.00; 0.01; 0.10; 1.00; 10.00 and 100.00 mg L-1) during 48h, and chronic toxicity tests (0.00; 3.125; 6.25; 12.50; 25.00 and 50.00 mg L-1) during 14 days. Characterization of NPs in the exposure medium revealed that they experienced agglomeration and aggregation on a micrometer scale. Results showed non-toxicity of nano-Fe3O4 after 48h of acute exposure (EC5048h > 100.00 mg L-1). ). In chronic tests, treatment of 50 mg L-1 caused significant inhibition of growth and reproduction, affecting the maximum length (inhibited 12.71%), accumulated number of eggs (reduction of 51.99%) and neonates (decrease of 61.37%) per female (Dunnett’s test, p < 0.05). We concluded that nano-Fe3O4, in the 14th day, was toxic to C. silvestrii only at the highest concentration tested (50.00 mg L-1) during this chronic exposure.. The use of nano-Fe3O4 in aquatic environments could be considered safe to this species in concentrations up to 25.00 mg L-1, according to the parameters evaluated. |
publishDate |
2017 |
dc.date.accessioned.fl_str_mv |
2017-08-22T14:00:38Z |
dc.date.available.fl_str_mv |
2017-08-22T14:00:38Z |
dc.date.issued.fl_str_mv |
2017-03-02 |
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info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
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masterThesis |
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publishedVersion |
dc.identifier.citation.fl_str_mv |
GEBARA, Renan Castelhano. Toxicidade de nanopartículas de óxido de ferro (Fe3O4) para o cladócero tropical Ceriodaphnia silvestrii. 2017. Dissertação (Mestrado em Ecologia e Recursos Naturais) – Universidade Federal de São Carlos, São Carlos, 2017. Disponível em: https://repositorio.ufscar.br/handle/ufscar/9045. |
dc.identifier.uri.fl_str_mv |
https://repositorio.ufscar.br/handle/ufscar/9045 |
identifier_str_mv |
GEBARA, Renan Castelhano. Toxicidade de nanopartículas de óxido de ferro (Fe3O4) para o cladócero tropical Ceriodaphnia silvestrii. 2017. Dissertação (Mestrado em Ecologia e Recursos Naturais) – Universidade Federal de São Carlos, São Carlos, 2017. Disponível em: https://repositorio.ufscar.br/handle/ufscar/9045. |
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https://repositorio.ufscar.br/handle/ufscar/9045 |
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Universidade Federal de São Carlos Câmpus São Carlos |
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Programa de Pós-Graduação em Ecologia e Recursos Naturais - PPGERN |
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Universidade Federal de São Carlos Câmpus São Carlos |
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