Estudo da imobilização de celulase em géis de quitosana.
Autor(a) principal: | |
---|---|
Data de Publicação: | 2007 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFSCAR |
Texto Completo: | https://repositorio.ufscar.br/handle/ufscar/3978 |
Resumo: | Cellulases are enzymes that catalyze the hydrolysis of cellulose producing glucose. Immobilization and stabilization of these enzymes could improve enzymatic process making it more attractive by reducing costs during their use. In this work, Celluclast (Novozymes A/S, Denmark) and CG 200 (Genencor) were immobilized into chitosan and into a chitosan/alginate hybrid support, after activation of the support with glutaraldehyde and glycidol under different conditions of immobilization such as temperature and pH. Immobilization process was evaluated by calculating the immobilization yield, coupling yield and thermal stability at 65°C. Temperature and pH optimum of enzymatic activity were analyzed as well. The best derivatives were obtained with chitosan alginate activated with glycidol, after immobilization at 27°C, pH 7.0, for 16 hours. The biocatalyst presented 56.3% of immobilization yield, 40.6% of coupling yield and it 11.2 fold more stable than free enzyme at 65°C. The temperature and pH for maximum activity of the derivatives and soluble enzyme was 60°C and 50°C, pH 2.5-3.0 and 4.2, respectively. Finally, the performance of the best biocatalyst developed in this work in the hydrolysis of sugarcane bagasse was compared to one observed using soluble enzyme. Although a higher conversion has been obtained with soluble enzyme in the beginning of the reaction, the immobilized enzyme allowed to reach a higher final conversion, 70%, than the one obtained with free Celluclast, 38.9%. |
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Martins, Rafael EliasGiordano, Raquel de Lima Camargohttp://genos.cnpq.br:12010/dwlattes/owa/prc_imp_cv_int?f_cod=K4780181P0http://lattes.cnpq.br/0792538389469353e4685f16-ab0f-4b64-9cbe-803f7c6d83b62016-06-02T19:56:28Z2007-08-312016-06-02T19:56:28Z2007-01-29MARTINS, Rafael Elias. Estudo da imobilização de celulase em géis de quitosana.. 2007. 100 f. Dissertação (Mestrado em Ciências Exatas e da Terra) - Universidade Federal de São Carlos, São Carlos, 2007.https://repositorio.ufscar.br/handle/ufscar/3978Cellulases are enzymes that catalyze the hydrolysis of cellulose producing glucose. Immobilization and stabilization of these enzymes could improve enzymatic process making it more attractive by reducing costs during their use. In this work, Celluclast (Novozymes A/S, Denmark) and CG 200 (Genencor) were immobilized into chitosan and into a chitosan/alginate hybrid support, after activation of the support with glutaraldehyde and glycidol under different conditions of immobilization such as temperature and pH. Immobilization process was evaluated by calculating the immobilization yield, coupling yield and thermal stability at 65°C. Temperature and pH optimum of enzymatic activity were analyzed as well. The best derivatives were obtained with chitosan alginate activated with glycidol, after immobilization at 27°C, pH 7.0, for 16 hours. The biocatalyst presented 56.3% of immobilization yield, 40.6% of coupling yield and it 11.2 fold more stable than free enzyme at 65°C. The temperature and pH for maximum activity of the derivatives and soluble enzyme was 60°C and 50°C, pH 2.5-3.0 and 4.2, respectively. Finally, the performance of the best biocatalyst developed in this work in the hydrolysis of sugarcane bagasse was compared to one observed using soluble enzyme. Although a higher conversion has been obtained with soluble enzyme in the beginning of the reaction, the immobilized enzyme allowed to reach a higher final conversion, 70%, than the one obtained with free Celluclast, 38.9%.Celulases catalisam hidrólise de celulose à glicose e a imobilização e estabilização da mesma podem viabilizar o processo enzimático, pois reduz custos em sua utilização. Neste trabalho, Celluclast 1.5L (Novozymes A/S, Denmark) e CG 200 (Genencor) foram imobilizadas em géis de quitosana e quitosana-alginato ativados com glutaraldeído e glicidol para posterior hidrólise do bagaço de cana-de-açúcar. O processo de imobilização foi acompanhado medindo-se proteína e atividade hidrolítica da enzima em papel de filtro. Avaliaram-se rendimento de imobilização, atividade recuperada, estabilidade térmica, temperatura e pH ótimo de atividade enzimática. Os melhores derivados foram obtidos com quitosana-alginato, ativados com glicidol com temperatura de imobilização de 27°C e pH 7,0 sendo 11,2 vezes mais estáveis que a enzima livre, com rendimento em torno de 40,6% e atividade recuperada em torno de 56,3%. A temperatura ótima para os derivados foi cerca de 10 graus maior que a da enzima livre (50°C), enquanto que o pH deslocou-se de 4,2 (enzima livre) para a região entre 2,5 e 3, para os derivados. Com o melhor derivado, realizou-se a hidrólise do bagaço de cana-de-açúcar, que apresentou conversão de 1,8 vezes (70,0%) superior à enzima livre (38,9%).application/pdfporUniversidade Federal de São CarlosPrograma de Pós-Graduação em Engenharia Química - PPGEQUFSCarBRTecnologia de enzimasCelulaseImobilizaçãoQuitosanaBagaço de canacellulaseimmobilizationchitosan, sodium alginate, sugarcane bagasseENGENHARIAS::ENGENHARIA QUIMICAEstudo da imobilização de celulase em géis de quitosana.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis-1-187b60e6c-591e-4a38-94f3-e75e2beebea0info:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFSCARinstname:Universidade Federal de São Carlos (UFSCAR)instacron:UFSCARORIGINALDissREM.pdfapplication/pdf1870524https://repositorio.ufscar.br/bitstream/ufscar/3978/1/DissREM.pdf11256ff68568004b18adba99fa4ba12bMD51THUMBNAILDissREM.pdf.jpgDissREM.pdf.jpgIM Thumbnailimage/jpeg6286https://repositorio.ufscar.br/bitstream/ufscar/3978/2/DissREM.pdf.jpg752013505321372725b97ec796fabe04MD52ufscar/39782023-09-18 18:30:58.923oai:repositorio.ufscar.br:ufscar/3978Repositório InstitucionalPUBhttps://repositorio.ufscar.br/oai/requestopendoar:43222023-09-18T18:30:58Repositório Institucional da UFSCAR - Universidade Federal de São Carlos (UFSCAR)false |
dc.title.por.fl_str_mv |
Estudo da imobilização de celulase em géis de quitosana. |
title |
Estudo da imobilização de celulase em géis de quitosana. |
spellingShingle |
Estudo da imobilização de celulase em géis de quitosana. Martins, Rafael Elias Tecnologia de enzimas Celulase Imobilização Quitosana Bagaço de cana cellulase immobilization chitosan, sodium alginate, sugarcane bagasse ENGENHARIAS::ENGENHARIA QUIMICA |
title_short |
Estudo da imobilização de celulase em géis de quitosana. |
title_full |
Estudo da imobilização de celulase em géis de quitosana. |
title_fullStr |
Estudo da imobilização de celulase em géis de quitosana. |
title_full_unstemmed |
Estudo da imobilização de celulase em géis de quitosana. |
title_sort |
Estudo da imobilização de celulase em géis de quitosana. |
author |
Martins, Rafael Elias |
author_facet |
Martins, Rafael Elias |
author_role |
author |
dc.contributor.authorlattes.por.fl_str_mv |
http://lattes.cnpq.br/0792538389469353 |
dc.contributor.author.fl_str_mv |
Martins, Rafael Elias |
dc.contributor.advisor1.fl_str_mv |
Giordano, Raquel de Lima Camargo |
dc.contributor.advisor1Lattes.fl_str_mv |
http://genos.cnpq.br:12010/dwlattes/owa/prc_imp_cv_int?f_cod=K4780181P0 |
dc.contributor.authorID.fl_str_mv |
e4685f16-ab0f-4b64-9cbe-803f7c6d83b6 |
contributor_str_mv |
Giordano, Raquel de Lima Camargo |
dc.subject.por.fl_str_mv |
Tecnologia de enzimas Celulase Imobilização Quitosana Bagaço de cana |
topic |
Tecnologia de enzimas Celulase Imobilização Quitosana Bagaço de cana cellulase immobilization chitosan, sodium alginate, sugarcane bagasse ENGENHARIAS::ENGENHARIA QUIMICA |
dc.subject.eng.fl_str_mv |
cellulase immobilization chitosan, sodium alginate, sugarcane bagasse |
dc.subject.cnpq.fl_str_mv |
ENGENHARIAS::ENGENHARIA QUIMICA |
description |
Cellulases are enzymes that catalyze the hydrolysis of cellulose producing glucose. Immobilization and stabilization of these enzymes could improve enzymatic process making it more attractive by reducing costs during their use. In this work, Celluclast (Novozymes A/S, Denmark) and CG 200 (Genencor) were immobilized into chitosan and into a chitosan/alginate hybrid support, after activation of the support with glutaraldehyde and glycidol under different conditions of immobilization such as temperature and pH. Immobilization process was evaluated by calculating the immobilization yield, coupling yield and thermal stability at 65°C. Temperature and pH optimum of enzymatic activity were analyzed as well. The best derivatives were obtained with chitosan alginate activated with glycidol, after immobilization at 27°C, pH 7.0, for 16 hours. The biocatalyst presented 56.3% of immobilization yield, 40.6% of coupling yield and it 11.2 fold more stable than free enzyme at 65°C. The temperature and pH for maximum activity of the derivatives and soluble enzyme was 60°C and 50°C, pH 2.5-3.0 and 4.2, respectively. Finally, the performance of the best biocatalyst developed in this work in the hydrolysis of sugarcane bagasse was compared to one observed using soluble enzyme. Although a higher conversion has been obtained with soluble enzyme in the beginning of the reaction, the immobilized enzyme allowed to reach a higher final conversion, 70%, than the one obtained with free Celluclast, 38.9%. |
publishDate |
2007 |
dc.date.available.fl_str_mv |
2007-08-31 2016-06-02T19:56:28Z |
dc.date.issued.fl_str_mv |
2007-01-29 |
dc.date.accessioned.fl_str_mv |
2016-06-02T19:56:28Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
MARTINS, Rafael Elias. Estudo da imobilização de celulase em géis de quitosana.. 2007. 100 f. Dissertação (Mestrado em Ciências Exatas e da Terra) - Universidade Federal de São Carlos, São Carlos, 2007. |
dc.identifier.uri.fl_str_mv |
https://repositorio.ufscar.br/handle/ufscar/3978 |
identifier_str_mv |
MARTINS, Rafael Elias. Estudo da imobilização de celulase em géis de quitosana.. 2007. 100 f. Dissertação (Mestrado em Ciências Exatas e da Terra) - Universidade Federal de São Carlos, São Carlos, 2007. |
url |
https://repositorio.ufscar.br/handle/ufscar/3978 |
dc.language.iso.fl_str_mv |
por |
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por |
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-1 -1 |
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87b60e6c-591e-4a38-94f3-e75e2beebea0 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de São Carlos |
dc.publisher.program.fl_str_mv |
Programa de Pós-Graduação em Engenharia Química - PPGEQ |
dc.publisher.initials.fl_str_mv |
UFSCar |
dc.publisher.country.fl_str_mv |
BR |
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Universidade Federal de São Carlos |
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