Investigação do potencial antimicrobiano de Casearia sylvestris sobre microrganismo endossimbionte de Diabrotica speciosa
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFSCAR |
Texto Completo: | https://repositorio.ufscar.br/handle/ufscar/6556 |
Resumo: | Diabrotica speciosa is a pest insect which occurs in most Brazilian states, standing out as one of the most important polyphagous pest of in grain crops. The understand of the role of the microbiota present in the digestive tract and of this insect the identification of ways to interfer or even to eliminate this microbial flora they are knowlegdes that, may lead mode of action to control of the pest insect. In this context, this study aimed to evaluate the microbial diversity present in the digestive tract of larvae and adults of D. speciosa and in the sometime, to investigate the potential of C. sylvestris as an antimicrobial agent. As results, they were obtained 21 bacterial isolates and 8 fungal isolates. The proteic profile of these microrganisms were analyzed by using MALDI-TOF/MS. Afterwards, these data were grouped identifying clusters of isolates. A bacteria each groups was identified by rDNA sequencing, 16S region. Identified bacteria were: Pantoea agglomerans, Luteibacter sp., Serratia marcescens, and Pseudomonas temperans Acidovorax sp. Two isolated fungi were identified through morphological comparison with literature data, and identified as Cladosporium sp. and Penicillium sp. A further study was carried out with Luteibacter sp bacteria, which were isolated from D. speciosa larva. In this study was evaluated growth profile and the parameters to carry out the antimicrobial assay. Several kind of microbial assays were investigated such as agar diffusion and microdilution plate ELISA®. The second method showed the best results. Thus, a microdiluition methodology using fluorometric assay, was developed and validated, highlighting parameter such as linearity, specificity, accuracy, precision, robustness, recovery, limites of detection, quantification and detection, etc. This method was applied evaluating extracts, fraction and secondary molecules of cerrado plants. Phytochemical study of C. sylvestris leaves resulted in the isolation of an umpublished tricyclic sesquiterpene, the flavonoid catechin and a clerodane diterpene, which was used as a reference marker in chromatographic method to quality control of the extracts and fractions of C. sylvestris that were used in assays. |
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Marques, Fabiana AparecidaForim, Moacir Rossihttp://lattes.cnpq.br/1234565160573730http://lattes.cnpq.br/1362671256884870d0e09c53-fee9-40a7-8d9d-5555a6c322132016-06-02T20:36:45Z2013-07-012016-06-02T20:36:45Z2013-03-27MARQUES, Fabiana Aparecida. Investigation of the potential antimicrobial of casearia sylvestris on the endosymbiont microorganisms of diabrotica speciosa. 2013. 195 f. Dissertação (Mestrado em Ciências Exatas e da Terra) - Universidade Federal de São Carlos, São Carlos, 2013.https://repositorio.ufscar.br/handle/ufscar/6556Diabrotica speciosa is a pest insect which occurs in most Brazilian states, standing out as one of the most important polyphagous pest of in grain crops. The understand of the role of the microbiota present in the digestive tract and of this insect the identification of ways to interfer or even to eliminate this microbial flora they are knowlegdes that, may lead mode of action to control of the pest insect. In this context, this study aimed to evaluate the microbial diversity present in the digestive tract of larvae and adults of D. speciosa and in the sometime, to investigate the potential of C. sylvestris as an antimicrobial agent. As results, they were obtained 21 bacterial isolates and 8 fungal isolates. The proteic profile of these microrganisms were analyzed by using MALDI-TOF/MS. Afterwards, these data were grouped identifying clusters of isolates. A bacteria each groups was identified by rDNA sequencing, 16S region. Identified bacteria were: Pantoea agglomerans, Luteibacter sp., Serratia marcescens, and Pseudomonas temperans Acidovorax sp. Two isolated fungi were identified through morphological comparison with literature data, and identified as Cladosporium sp. and Penicillium sp. A further study was carried out with Luteibacter sp bacteria, which were isolated from D. speciosa larva. In this study was evaluated growth profile and the parameters to carry out the antimicrobial assay. Several kind of microbial assays were investigated such as agar diffusion and microdilution plate ELISA®. The second method showed the best results. Thus, a microdiluition methodology using fluorometric assay, was developed and validated, highlighting parameter such as linearity, specificity, accuracy, precision, robustness, recovery, limites of detection, quantification and detection, etc. This method was applied evaluating extracts, fraction and secondary molecules of cerrado plants. Phytochemical study of C. sylvestris leaves resulted in the isolation of an umpublished tricyclic sesquiterpene, the flavonoid catechin and a clerodane diterpene, which was used as a reference marker in chromatographic method to quality control of the extracts and fractions of C. sylvestris that were used in assays.A Diabrotica speciosa é uma espécie que ocorre na maioria dos estados brasileiros, destacando-se como uma das principais pragas polífagas nas culturas de grãos. O entendimento da função da microbiota presente no trato digestivo deste inseto e a identificação de meios de interferir ou até mesmo destruir essa flora microbiana são conhecimentos que podem levar a um novo modo de ação para o controle de insetos praga. Neste contexto, este trabalho teve como objetivo avaliar a diversidade microbiana presente no trato digestivo de larvas e insetos adultos de D. speciosa e avaliar o potencial de Casearia sylvestris e extratos de plantas do cerrado como agente antimicrobiano. Como resultados, foram obtidos 19 isolados bacterianos e 8 fúngicos. Estes foram avaliados em termos de perfis protéico por MALDI-TOF/MS, e após a obtenção de agrupamentos estatísticos alguns microrganismos foram identificadas através do seqüenciamento de rDNA região 16S. As bactérias identificadas foram: Pantoea agglomerans, Luteibacter sp., Serratia marcescens, Acidovorax temperans e Pseudomonas sp. Dos isolados fúngicos, dois foram identificados através de comparação morfológica como dados da literatura, pertencendo aos gêneros; Cladosporium sp. e Penicillium sp. Um estudo mais aprofundado foi realizado com a bactéria Luteibacter sp isolada da larva, que teve seu perfil de crescimento avaliado, possibilitando o ajuste de parâmetros para realização de ensaios antimicrobiano. Para estes ensaios foram testadas metodologias de difusão em ágar e por microdiluição em placa de ELISA®, sendo na segunda metodologia obtido os melhores resultados. Assim, uma metodologia por microdiluição foi validada através da utilização do fluorímetro, analisando figuras de mérito como faixa linear de trabalho, precisão, exatidão, limites de quantificação e detecção, etc. O método por microdiluição foi aplicado ensaiando extratos, frações e metabólitos de plantas do cerrado. O estudo fitoquímico das folhas de C. sylvestris propiciou o isolamento de um sesquiterpeno tricíclico inédito na literatura, um flavonóide (catequina) e um diterpeno clerodânico que foi utilizado como marcador de referência para controle cromatográfico de qualidade de extratos e frações C sylvestris que foram submetidos a ensaios antimicrobianos.Financiadora de Estudos e Projetosapplication/pdfporUniversidade Federal de São CarlosPrograma de Pós-Graduação em Química - PPGQUFSCarBRQuímica orgânicaMicroorganismos endossimbiontesDiabrotica speciosaAntimicrobianaCasearia sylvestrisFitoquímicaPlantas do cerradoEndosymbiont microorganismsDiabrotica speciosaCerrado plantsAntimicrobial activityCasearia sylvestrisPhytochemistryCIENCIAS EXATAS E DA TERRA::QUIMICAInvestigação do potencial antimicrobiano de Casearia sylvestris sobre microrganismo endossimbionte de Diabrotica speciosaInvestigation of the potential antimicrobial of casearia sylvestris on the endosymbiont microorganisms of diabrotica speciosainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis-1-1479a949d-29af-46e4-bd97-337dd2c96228info:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFSCARinstname:Universidade Federal de São Carlos (UFSCAR)instacron:UFSCARORIGINAL5223.pdfapplication/pdf14772341https://repositorio.ufscar.br/bitstream/ufscar/6556/1/5223.pdff9288ce2d5f0b661781b49aa3722146bMD51TEXT5223.pdf.txt5223.pdf.txtExtracted texttext/plain0https://repositorio.ufscar.br/bitstream/ufscar/6556/4/5223.pdf.txtd41d8cd98f00b204e9800998ecf8427eMD54THUMBNAIL5223.pdf.jpg5223.pdf.jpgIM Thumbnailimage/jpeg8912https://repositorio.ufscar.br/bitstream/ufscar/6556/5/5223.pdf.jpg59fbdd4c7e5a50d718fb435140c0916eMD55ufscar/65562023-09-18 18:30:39.717oai:repositorio.ufscar.br:ufscar/6556Repositório InstitucionalPUBhttps://repositorio.ufscar.br/oai/requestopendoar:43222023-09-18T18:30:39Repositório Institucional da UFSCAR - Universidade Federal de São Carlos (UFSCAR)false |
dc.title.por.fl_str_mv |
Investigação do potencial antimicrobiano de Casearia sylvestris sobre microrganismo endossimbionte de Diabrotica speciosa |
dc.title.alternative.eng.fl_str_mv |
Investigation of the potential antimicrobial of casearia sylvestris on the endosymbiont microorganisms of diabrotica speciosa |
title |
Investigação do potencial antimicrobiano de Casearia sylvestris sobre microrganismo endossimbionte de Diabrotica speciosa |
spellingShingle |
Investigação do potencial antimicrobiano de Casearia sylvestris sobre microrganismo endossimbionte de Diabrotica speciosa Marques, Fabiana Aparecida Química orgânica Microorganismos endossimbiontes Diabrotica speciosa Antimicrobiana Casearia sylvestris Fitoquímica Plantas do cerrado Endosymbiont microorganisms Diabrotica speciosa Cerrado plants Antimicrobial activity Casearia sylvestris Phytochemistry CIENCIAS EXATAS E DA TERRA::QUIMICA |
title_short |
Investigação do potencial antimicrobiano de Casearia sylvestris sobre microrganismo endossimbionte de Diabrotica speciosa |
title_full |
Investigação do potencial antimicrobiano de Casearia sylvestris sobre microrganismo endossimbionte de Diabrotica speciosa |
title_fullStr |
Investigação do potencial antimicrobiano de Casearia sylvestris sobre microrganismo endossimbionte de Diabrotica speciosa |
title_full_unstemmed |
Investigação do potencial antimicrobiano de Casearia sylvestris sobre microrganismo endossimbionte de Diabrotica speciosa |
title_sort |
Investigação do potencial antimicrobiano de Casearia sylvestris sobre microrganismo endossimbionte de Diabrotica speciosa |
author |
Marques, Fabiana Aparecida |
author_facet |
Marques, Fabiana Aparecida |
author_role |
author |
dc.contributor.authorlattes.por.fl_str_mv |
http://lattes.cnpq.br/1362671256884870 |
dc.contributor.author.fl_str_mv |
Marques, Fabiana Aparecida |
dc.contributor.advisor1.fl_str_mv |
Forim, Moacir Rossi |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/1234565160573730 |
dc.contributor.authorID.fl_str_mv |
d0e09c53-fee9-40a7-8d9d-5555a6c32213 |
contributor_str_mv |
Forim, Moacir Rossi |
dc.subject.por.fl_str_mv |
Química orgânica Microorganismos endossimbiontes Diabrotica speciosa Antimicrobiana Casearia sylvestris Fitoquímica Plantas do cerrado |
topic |
Química orgânica Microorganismos endossimbiontes Diabrotica speciosa Antimicrobiana Casearia sylvestris Fitoquímica Plantas do cerrado Endosymbiont microorganisms Diabrotica speciosa Cerrado plants Antimicrobial activity Casearia sylvestris Phytochemistry CIENCIAS EXATAS E DA TERRA::QUIMICA |
dc.subject.eng.fl_str_mv |
Endosymbiont microorganisms Diabrotica speciosa Cerrado plants Antimicrobial activity Casearia sylvestris Phytochemistry |
dc.subject.cnpq.fl_str_mv |
CIENCIAS EXATAS E DA TERRA::QUIMICA |
description |
Diabrotica speciosa is a pest insect which occurs in most Brazilian states, standing out as one of the most important polyphagous pest of in grain crops. The understand of the role of the microbiota present in the digestive tract and of this insect the identification of ways to interfer or even to eliminate this microbial flora they are knowlegdes that, may lead mode of action to control of the pest insect. In this context, this study aimed to evaluate the microbial diversity present in the digestive tract of larvae and adults of D. speciosa and in the sometime, to investigate the potential of C. sylvestris as an antimicrobial agent. As results, they were obtained 21 bacterial isolates and 8 fungal isolates. The proteic profile of these microrganisms were analyzed by using MALDI-TOF/MS. Afterwards, these data were grouped identifying clusters of isolates. A bacteria each groups was identified by rDNA sequencing, 16S region. Identified bacteria were: Pantoea agglomerans, Luteibacter sp., Serratia marcescens, and Pseudomonas temperans Acidovorax sp. Two isolated fungi were identified through morphological comparison with literature data, and identified as Cladosporium sp. and Penicillium sp. A further study was carried out with Luteibacter sp bacteria, which were isolated from D. speciosa larva. In this study was evaluated growth profile and the parameters to carry out the antimicrobial assay. Several kind of microbial assays were investigated such as agar diffusion and microdilution plate ELISA®. The second method showed the best results. Thus, a microdiluition methodology using fluorometric assay, was developed and validated, highlighting parameter such as linearity, specificity, accuracy, precision, robustness, recovery, limites of detection, quantification and detection, etc. This method was applied evaluating extracts, fraction and secondary molecules of cerrado plants. Phytochemical study of C. sylvestris leaves resulted in the isolation of an umpublished tricyclic sesquiterpene, the flavonoid catechin and a clerodane diterpene, which was used as a reference marker in chromatographic method to quality control of the extracts and fractions of C. sylvestris that were used in assays. |
publishDate |
2013 |
dc.date.available.fl_str_mv |
2013-07-01 2016-06-02T20:36:45Z |
dc.date.issued.fl_str_mv |
2013-03-27 |
dc.date.accessioned.fl_str_mv |
2016-06-02T20:36:45Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
MARQUES, Fabiana Aparecida. Investigation of the potential antimicrobial of casearia sylvestris on the endosymbiont microorganisms of diabrotica speciosa. 2013. 195 f. Dissertação (Mestrado em Ciências Exatas e da Terra) - Universidade Federal de São Carlos, São Carlos, 2013. |
dc.identifier.uri.fl_str_mv |
https://repositorio.ufscar.br/handle/ufscar/6556 |
identifier_str_mv |
MARQUES, Fabiana Aparecida. Investigation of the potential antimicrobial of casearia sylvestris on the endosymbiont microorganisms of diabrotica speciosa. 2013. 195 f. Dissertação (Mestrado em Ciências Exatas e da Terra) - Universidade Federal de São Carlos, São Carlos, 2013. |
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https://repositorio.ufscar.br/handle/ufscar/6556 |
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openAccess |
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Universidade Federal de São Carlos |
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Programa de Pós-Graduação em Química - PPGQ |
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UFSCar |
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BR |
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Universidade Federal de São Carlos |
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