Avaliação da atividade biológica do material orgânico derivado da base de Schiff N,N-bis'(salicilideno)-1,2-fenilenodiamina complexado com cobre (ll) e cobalto (ll)
Autor(a) principal: | |
---|---|
Data de Publicação: | 2021 |
Tipo de documento: | Tese |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFSCAR |
Texto Completo: | https://repositorio.ufscar.br/handle/ufscar/16063 |
Resumo: | Treatment for bacterial infections is through antimicrobials and resistance is a major worldwide problem that leads to approximately 35,000 deaths per year. Another public health problem is related to occurrences caused by parasites such as visceral leishmaniasis, which presents severe clinical manifestations that can lead to death and the available treatments can cause definite side effects in addition to resistance. In this way, there is a need to produce new substances for the pharmacological development of molecules and their derivatives. This study aimed to evaluate the physical and biological properties of products derived from the base of Schiff N, N’-bis (Salicylidene) -1,2-phenylenediamine complexed with copper (II) and cobalt (II). X-ray diffraction techniques, Ramam spectroscopy, Minimum Inhibitory Concentration (MIC), Minimum Bactericidal Concentration (CBM), time kill, modulation of antibiotics with amikacin and gentamicin, determination of leishmanicida, determination of cell cytotoxicity in fibroblasts and evaluation of production of Reactive Oxygen Species (ROS). It was possible to identify differences between the free ligand and its criteria. In the antimicrobial activity, the most expressive results were obtained with the Gram-positives S. aureus, S. epidermidis, S. saprophyticus, M. luteus, E. faecalis and B. subitilis with inhibitions between 75 and 96% for all tested and a MIC of 125 µg.ml-1 to these bacteria. The CBM obtained was 1000 µg.ml-1 and 250 µg.ml-1 to S. aureus and S. epidermidis, respectively. To S. saprophyticus and E. faecalis, 500 µg.ml- 1 was detected. Through the antibiotic modulation test, a synergistic effect of the free ligand with the antibiotics gentamicin and amikacin was detected, reducing the MIC by 4 and 16X. In the evaluation of the leishmanicidal activity, activity was observed in the highest tested concentration of the studies with inhibitions above 85%. The assessment of cytotoxicity allowed us to observe that the free ligand has lower toxicity for all cell types tested when compared to the complexes. Through the ROS test, ROS production was detected, but at the lowest concentration tested, this production reached levels close to the levels of the control group. Through the cell migration test, total cell growth was detected in 48 hours with the free ligand and 72 hours with the cobalt complex. Thus, the data suggest that the cobalt complex and the free ligand have expressive antimicrobial and leishmanicidal activity when compared to the copper complex. |
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Soriano, BiancaSousa, Cristina Paiva dehttp://lattes.cnpq.br/9002619114161319http://lattes.cnpq.br/6431092009628550ea0da443-0400-454b-9b5f-e0eebc9cf35e2022-05-06T21:47:31Z2022-05-06T21:47:31Z2021-11-08SORIANO, Bianca. Avaliação da atividade biológica do material orgânico derivado da base de Schiff N,N-bis'(salicilideno)-1,2-fenilenodiamina complexado com cobre (ll) e cobalto (ll). 2021. Tese (Doutorado em Biotecnologia) – Universidade Federal de São Carlos, São Carlos, 2021. Disponível em: https://repositorio.ufscar.br/handle/ufscar/16063.https://repositorio.ufscar.br/handle/ufscar/16063Treatment for bacterial infections is through antimicrobials and resistance is a major worldwide problem that leads to approximately 35,000 deaths per year. Another public health problem is related to occurrences caused by parasites such as visceral leishmaniasis, which presents severe clinical manifestations that can lead to death and the available treatments can cause definite side effects in addition to resistance. In this way, there is a need to produce new substances for the pharmacological development of molecules and their derivatives. This study aimed to evaluate the physical and biological properties of products derived from the base of Schiff N, N’-bis (Salicylidene) -1,2-phenylenediamine complexed with copper (II) and cobalt (II). X-ray diffraction techniques, Ramam spectroscopy, Minimum Inhibitory Concentration (MIC), Minimum Bactericidal Concentration (CBM), time kill, modulation of antibiotics with amikacin and gentamicin, determination of leishmanicida, determination of cell cytotoxicity in fibroblasts and evaluation of production of Reactive Oxygen Species (ROS). It was possible to identify differences between the free ligand and its criteria. In the antimicrobial activity, the most expressive results were obtained with the Gram-positives S. aureus, S. epidermidis, S. saprophyticus, M. luteus, E. faecalis and B. subitilis with inhibitions between 75 and 96% for all tested and a MIC of 125 µg.ml-1 to these bacteria. The CBM obtained was 1000 µg.ml-1 and 250 µg.ml-1 to S. aureus and S. epidermidis, respectively. To S. saprophyticus and E. faecalis, 500 µg.ml- 1 was detected. Through the antibiotic modulation test, a synergistic effect of the free ligand with the antibiotics gentamicin and amikacin was detected, reducing the MIC by 4 and 16X. In the evaluation of the leishmanicidal activity, activity was observed in the highest tested concentration of the studies with inhibitions above 85%. The assessment of cytotoxicity allowed us to observe that the free ligand has lower toxicity for all cell types tested when compared to the complexes. Through the ROS test, ROS production was detected, but at the lowest concentration tested, this production reached levels close to the levels of the control group. Through the cell migration test, total cell growth was detected in 48 hours with the free ligand and 72 hours with the cobalt complex. Thus, the data suggest that the cobalt complex and the free ligand have expressive antimicrobial and leishmanicidal activity when compared to the copper complex.O tratamento para as infecções bacterianas é feito através de antimicrobianos e a resistência é um grande problema mundial que leva a aproximadamente 35.000 mortes por ano. Outro problema de saúde pública está relacionado as infecções ocasionadas por parasitas como a leishmaniose visceral que apresenta manifestações clínicas graves podendo levar a óbito e os tratamentos disponíveis podem ocasionar efeitos colaterais significativos além de resistência. Em vista deste contexto há necessidade de produção de novas substancias para o desenvolvimento farmacológico de moléculas e seus derivados. Este estudo teve como objetivo avaliar as propriedades físicas e biológicas dos derivados orgânicos da base de Schiff N,N’-bis(Salicilideno)-1,2- fenilenodiamina complexada com cobre (II) e cobalto (II). Foram utilizadas técnicas de difração de raios X, espectroscopia Ramam, Concentração Inibitória Mínima (CIM), Concentração Bactericida Mínima (CBM), time kill, modulação de antibióticos com amicacina e gentamicina, determinação da atividade leishmanicida, determinação da citotoxicidade celular em fibroblastos, avaliação da produção de Espécies Reativas de oxigênio (EROs) e migração celular. Foi possível detectar diferenças estruturais entre o ligante livre e seus complexos. Na atividade antimicrobiana, os resultados mais expressivos foram obtidos com as Gram-positivas S. aureus, S. epidermidis, S. saprophyticus, M. luteus, E. faecalis e B. subtilis com inibições entre 75 e 96% para todas as concentrações testadas e uma CIM de 125 µg.ml-1 para estas bactérias. A CBM obtida foi de 1000 µg.ml-1 e 250 µg.ml-1 para S. aureus e S. epidermidis, respectivamente. Para S. saprophyticus e E. faecalis detectou-se 500 µg.ml-1. Através do teste de modulação de antibióticos detectou-se efeito sinérgico do ligante livre com os antibióticos gentamicina e amicacina diminuindo 4 e 16X a CIM. Na avaliação da atividade leishmanicida observou-se atividade nas maiores concentrações testadas dos complexos com inibições acima de 85%. A avaliação de citotoxicidade permitiu observar que o ligante livre apresenta menor toxicidade para todos os tipos celulares testados quando comparado aos complexos. Atraves do teste de ROS, detectou-se produção de EROs, porem na menor concentração testada, esta produção atingiu níveis próximos aos níveis do grupo controle. Atraves do teste de migração celular, detectou-se o crescimento total das células em 48 horas com o ligante livre e 72 horas com o complexo de cobalto. Sendo assim, os dados sugerem que o complexo de cobalto e o ligante livre apresentam atividade antimicrobiana e leishmanicida expressiva quando comparado com o complexo de cobre.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)88882.426509/2019-01porUniversidade Federal de São CarlosCâmpus São CarlosPrograma de Pós-Graduação em Biotecnologia - PPGBiotecUFSCarAttribution-NonCommercial-NoDerivs 3.0 Brazilhttp://creativecommons.org/licenses/by-nc-nd/3.0/br/info:eu-repo/semantics/openAccessAtividade antimicrobianaAtividade LeishmanicidaBase de SchiffComplexos metálicosAntimicrobial activityLeishmanicidal activitySchiff's baseMetal complexesCIENCIAS BIOLOGICAS::MICROBIOLOGIAAvaliação da atividade biológica do material orgânico derivado da base de Schiff N,N-bis'(salicilideno)-1,2-fenilenodiamina complexado com cobre (ll) e cobalto (ll)Evaluation of the biological activity of organic material derived from the Schiff base N,N-bis'(salicylidene)-1,2-phenylenediamine complexed with copper (ll) and cobalt (ll)info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesis60060035b91dbf-6130-418b-915b-3380dadd62dcreponame:Repositório Institucional da UFSCARinstname:Universidade Federal de São Carlos (UFSCAR)instacron:UFSCARORIGINALManuscrito Bianca Soriano.pdfManuscrito Bianca Soriano.pdfTese de Doutoradoapplication/pdf2550410https://repositorio.ufscar.br/bitstream/ufscar/16063/2/Manuscrito%20Bianca%20Soriano.pdf1c4cd8bc2611d804fd0aee9ef5ebc4fdMD52Carta Comprovante - Orientador.pdfCarta Comprovante - Orientador.pdfCarta comprovante orientadoraapplication/pdf292031https://repositorio.ufscar.br/bitstream/ufscar/16063/1/Carta%20Comprovante%20-%20Orientador.pdfdc111999552914eaf89e0303760f20b1MD51CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8811https://repositorio.ufscar.br/bitstream/ufscar/16063/3/license_rdfe39d27027a6cc9cb039ad269a5db8e34MD53TEXTManuscrito Bianca Soriano.pdf.txtManuscrito Bianca Soriano.pdf.txtExtracted texttext/plain166632https://repositorio.ufscar.br/bitstream/ufscar/16063/4/Manuscrito%20Bianca%20Soriano.pdf.txtbe64d8db829ed041932dd29e9210f98aMD54Carta Comprovante - Orientador.pdf.txtCarta Comprovante - Orientador.pdf.txtExtracted texttext/plain1547https://repositorio.ufscar.br/bitstream/ufscar/16063/6/Carta%20Comprovante%20-%20Orientador.pdf.txt330debd128760dfe76bfb3b090908ca2MD56THUMBNAILManuscrito Bianca Soriano.pdf.jpgManuscrito Bianca Soriano.pdf.jpgIM Thumbnailimage/jpeg5361https://repositorio.ufscar.br/bitstream/ufscar/16063/5/Manuscrito%20Bianca%20Soriano.pdf.jpg858b55323efba8b7dd876f4f7ecff186MD55Carta Comprovante - Orientador.pdf.jpgCarta Comprovante - Orientador.pdf.jpgIM Thumbnailimage/jpeg6008https://repositorio.ufscar.br/bitstream/ufscar/16063/7/Carta%20Comprovante%20-%20Orientador.pdf.jpg37a2068b50dccf9b639c5bfbb0dc2a07MD57ufscar/160632023-09-18 18:32:25.44oai:repositorio.ufscar.br:ufscar/16063Repositório InstitucionalPUBhttps://repositorio.ufscar.br/oai/requestopendoar:43222023-09-18T18:32:25Repositório Institucional da UFSCAR - Universidade Federal de São Carlos (UFSCAR)false |
dc.title.por.fl_str_mv |
Avaliação da atividade biológica do material orgânico derivado da base de Schiff N,N-bis'(salicilideno)-1,2-fenilenodiamina complexado com cobre (ll) e cobalto (ll) |
dc.title.alternative.eng.fl_str_mv |
Evaluation of the biological activity of organic material derived from the Schiff base N,N-bis'(salicylidene)-1,2-phenylenediamine complexed with copper (ll) and cobalt (ll) |
title |
Avaliação da atividade biológica do material orgânico derivado da base de Schiff N,N-bis'(salicilideno)-1,2-fenilenodiamina complexado com cobre (ll) e cobalto (ll) |
spellingShingle |
Avaliação da atividade biológica do material orgânico derivado da base de Schiff N,N-bis'(salicilideno)-1,2-fenilenodiamina complexado com cobre (ll) e cobalto (ll) Soriano, Bianca Atividade antimicrobiana Atividade Leishmanicida Base de Schiff Complexos metálicos Antimicrobial activity Leishmanicidal activity Schiff's base Metal complexes CIENCIAS BIOLOGICAS::MICROBIOLOGIA |
title_short |
Avaliação da atividade biológica do material orgânico derivado da base de Schiff N,N-bis'(salicilideno)-1,2-fenilenodiamina complexado com cobre (ll) e cobalto (ll) |
title_full |
Avaliação da atividade biológica do material orgânico derivado da base de Schiff N,N-bis'(salicilideno)-1,2-fenilenodiamina complexado com cobre (ll) e cobalto (ll) |
title_fullStr |
Avaliação da atividade biológica do material orgânico derivado da base de Schiff N,N-bis'(salicilideno)-1,2-fenilenodiamina complexado com cobre (ll) e cobalto (ll) |
title_full_unstemmed |
Avaliação da atividade biológica do material orgânico derivado da base de Schiff N,N-bis'(salicilideno)-1,2-fenilenodiamina complexado com cobre (ll) e cobalto (ll) |
title_sort |
Avaliação da atividade biológica do material orgânico derivado da base de Schiff N,N-bis'(salicilideno)-1,2-fenilenodiamina complexado com cobre (ll) e cobalto (ll) |
author |
Soriano, Bianca |
author_facet |
Soriano, Bianca |
author_role |
author |
dc.contributor.authorlattes.por.fl_str_mv |
http://lattes.cnpq.br/6431092009628550 |
dc.contributor.author.fl_str_mv |
Soriano, Bianca |
dc.contributor.advisor1.fl_str_mv |
Sousa, Cristina Paiva de |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/9002619114161319 |
dc.contributor.authorID.fl_str_mv |
ea0da443-0400-454b-9b5f-e0eebc9cf35e |
contributor_str_mv |
Sousa, Cristina Paiva de |
dc.subject.por.fl_str_mv |
Atividade antimicrobiana Atividade Leishmanicida Base de Schiff Complexos metálicos |
topic |
Atividade antimicrobiana Atividade Leishmanicida Base de Schiff Complexos metálicos Antimicrobial activity Leishmanicidal activity Schiff's base Metal complexes CIENCIAS BIOLOGICAS::MICROBIOLOGIA |
dc.subject.eng.fl_str_mv |
Antimicrobial activity Leishmanicidal activity Schiff's base Metal complexes |
dc.subject.cnpq.fl_str_mv |
CIENCIAS BIOLOGICAS::MICROBIOLOGIA |
description |
Treatment for bacterial infections is through antimicrobials and resistance is a major worldwide problem that leads to approximately 35,000 deaths per year. Another public health problem is related to occurrences caused by parasites such as visceral leishmaniasis, which presents severe clinical manifestations that can lead to death and the available treatments can cause definite side effects in addition to resistance. In this way, there is a need to produce new substances for the pharmacological development of molecules and their derivatives. This study aimed to evaluate the physical and biological properties of products derived from the base of Schiff N, N’-bis (Salicylidene) -1,2-phenylenediamine complexed with copper (II) and cobalt (II). X-ray diffraction techniques, Ramam spectroscopy, Minimum Inhibitory Concentration (MIC), Minimum Bactericidal Concentration (CBM), time kill, modulation of antibiotics with amikacin and gentamicin, determination of leishmanicida, determination of cell cytotoxicity in fibroblasts and evaluation of production of Reactive Oxygen Species (ROS). It was possible to identify differences between the free ligand and its criteria. In the antimicrobial activity, the most expressive results were obtained with the Gram-positives S. aureus, S. epidermidis, S. saprophyticus, M. luteus, E. faecalis and B. subitilis with inhibitions between 75 and 96% for all tested and a MIC of 125 µg.ml-1 to these bacteria. The CBM obtained was 1000 µg.ml-1 and 250 µg.ml-1 to S. aureus and S. epidermidis, respectively. To S. saprophyticus and E. faecalis, 500 µg.ml- 1 was detected. Through the antibiotic modulation test, a synergistic effect of the free ligand with the antibiotics gentamicin and amikacin was detected, reducing the MIC by 4 and 16X. In the evaluation of the leishmanicidal activity, activity was observed in the highest tested concentration of the studies with inhibitions above 85%. The assessment of cytotoxicity allowed us to observe that the free ligand has lower toxicity for all cell types tested when compared to the complexes. Through the ROS test, ROS production was detected, but at the lowest concentration tested, this production reached levels close to the levels of the control group. Through the cell migration test, total cell growth was detected in 48 hours with the free ligand and 72 hours with the cobalt complex. Thus, the data suggest that the cobalt complex and the free ligand have expressive antimicrobial and leishmanicidal activity when compared to the copper complex. |
publishDate |
2021 |
dc.date.issued.fl_str_mv |
2021-11-08 |
dc.date.accessioned.fl_str_mv |
2022-05-06T21:47:31Z |
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2022-05-06T21:47:31Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/doctoralThesis |
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doctoralThesis |
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publishedVersion |
dc.identifier.citation.fl_str_mv |
SORIANO, Bianca. Avaliação da atividade biológica do material orgânico derivado da base de Schiff N,N-bis'(salicilideno)-1,2-fenilenodiamina complexado com cobre (ll) e cobalto (ll). 2021. Tese (Doutorado em Biotecnologia) – Universidade Federal de São Carlos, São Carlos, 2021. Disponível em: https://repositorio.ufscar.br/handle/ufscar/16063. |
dc.identifier.uri.fl_str_mv |
https://repositorio.ufscar.br/handle/ufscar/16063 |
identifier_str_mv |
SORIANO, Bianca. Avaliação da atividade biológica do material orgânico derivado da base de Schiff N,N-bis'(salicilideno)-1,2-fenilenodiamina complexado com cobre (ll) e cobalto (ll). 2021. Tese (Doutorado em Biotecnologia) – Universidade Federal de São Carlos, São Carlos, 2021. Disponível em: https://repositorio.ufscar.br/handle/ufscar/16063. |
url |
https://repositorio.ufscar.br/handle/ufscar/16063 |
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por |
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Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ |
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openAccess |
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Universidade Federal de São Carlos Câmpus São Carlos |
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Programa de Pós-Graduação em Biotecnologia - PPGBiotec |
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UFSCar |
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Universidade Federal de São Carlos Câmpus São Carlos |
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