Initial evaluations of the use of eugenol as an antioxidant in the in vitro maturation of ovine oocytes
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , , , , , , , , , , |
Tipo de documento: | preprint |
Idioma: | por |
Título da fonte: | SciELO Preprints |
Texto Completo: | https://preprints.scielo.org/index.php/scielo/preprint/view/7755 |
Resumo: | The aim of this study was to assess the effect of eugenol on the in vitro production of ovine embryos. Oocytes were aspirated and taken for in vitro maturation (IVM), where they were divided into 4 groups: the CON (control), in which the oocytes were immersed in medium without an antioxidant; in the EU10, EU20, EU40 groups, the same medium as in the COM group was used, supplemented with 10, 20, and 40 µM of eugenol, respectively. The degree of expansion of cumulus cells was evaluated. Subsequently, the oocytes underwent in vitro fertilization and in vitro culture, and at the end of this process, the rate of cleaved structures was assessed. Regarding the expansion rate of cumulus cells, the CON group exhibited higher rates compared to the groups supplemented with eugenol. Evaluating oocytes with fully expanded cumulus cells, only the EU40 group showed a lower rate when compared to the rest of the treatment groups. Whereas observing oocytes with partially expanded cumulus cells, the EU40 group showed higher expansion percentages compared to the other treatments. Concerning the presence of the first polar body in the oocytes, it was observed that there was no statistical difference between the treatment groups. Lastly, concerning the cleaved structures, the CON and EU10 groups showed the highest rates compared to the EU20 and EU40 groups. Thus, it was possible to conclude that the use of 10 µM of eugenol in the IVM of ovine oocytes proved to be the optimal concentration of this substance. |
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Initial evaluations of the use of eugenol as an antioxidant in the in vitro maturation of ovine oocytesEvaluaciones iniciales del uso del eugenol como antioxidante en la maduración in vitro de ovocitos ovinosAvaliações iniciais do uso do eugenol como antioxidante na maturação in vitro de oócitos ovinosFIVMIVovelhaPIVzigotoIVFIVMIVPsheepzygotecigotoFIVMIVovejaPIVThe aim of this study was to assess the effect of eugenol on the in vitro production of ovine embryos. Oocytes were aspirated and taken for in vitro maturation (IVM), where they were divided into 4 groups: the CON (control), in which the oocytes were immersed in medium without an antioxidant; in the EU10, EU20, EU40 groups, the same medium as in the COM group was used, supplemented with 10, 20, and 40 µM of eugenol, respectively. The degree of expansion of cumulus cells was evaluated. Subsequently, the oocytes underwent in vitro fertilization and in vitro culture, and at the end of this process, the rate of cleaved structures was assessed. Regarding the expansion rate of cumulus cells, the CON group exhibited higher rates compared to the groups supplemented with eugenol. Evaluating oocytes with fully expanded cumulus cells, only the EU40 group showed a lower rate when compared to the rest of the treatment groups. Whereas observing oocytes with partially expanded cumulus cells, the EU40 group showed higher expansion percentages compared to the other treatments. Concerning the presence of the first polar body in the oocytes, it was observed that there was no statistical difference between the treatment groups. Lastly, concerning the cleaved structures, the CON and EU10 groups showed the highest rates compared to the EU20 and EU40 groups. Thus, it was possible to conclude that the use of 10 µM of eugenol in the IVM of ovine oocytes proved to be the optimal concentration of this substance.El objetivo del presente estudio fue evaluar el efecto del eugenol en la producción in vitro de embriones ovinos. Los ovocitos fueron aspirados y llevados para la maduración in vitro (MIV), donde se dividieron en 4 grupos: el CON (control), en el cual los ovocitos fueron sumergidos en medio sin antioxidantes; en los grupos EU10, EU20, EU40 se utilizó el mismo medio que en el grupo CON, pero con la adición de 10, 20 y 40 µM de eugenol, respectivamente. Se evaluó el grado de expansión de las células del cúmulo. Después de esto, los ovocitos fueron sometidos a la fecundación in vitro y al cultivo in vitro, y al final de este proceso se evaluó la tasa de estructuras clivadas. En cuanto a la tasa de expansión de las células del cúmulo, el grupo CON mostró tasas más altas en comparación con los grupos suplementados con eugenol. Al evaluar los ovocitos con células del cúmulo totalmente expandidas, solo el grupo EU40 mostró una tasa más baja en comparación con el resto de los grupos de tratamiento. Mientras que al observar los ovocitos con células del cúmulo parcialmente expandidas, el grupo EU40 mostró porcentajes de expansión más altos en comparación con los otros tratamientos. Respecto a la presencia del primer corpúsculo polar en los ovocitos, se observó que no hubo diferencia estadística entre los grupos de tratamiento. Por último, en relación con las estructuras clivadas, los grupos CON y EU10 mostraron las tasas más altas en comparación con los grupos EU20 y EU40. Por lo tanto, se pudo concluir que el uso de 10 µM de eugenol en la MIV de ovocitos ovinos resultó ser la concentración óptima de esta sustância.Objetivando avaliar o efeito do eugenol na produção in vitro de embriões ovinos. Os oócitos, foram aspirados e levados para a maturação in vitro (MIV), onde foram divididos em 4 grupos: o CON (controle), no qual os oócitos foram imersos em meio, sem antioxidante, já nos grupos EU10, EU20, EU40 foi utilizado o mesmo meio do grupo CON, acrescido de 10, 20 e 40 µM de eugenol, respectivamente. Foi avaliado o grau de expansão das células do cumulus. Após isso, os oócitos seguiram para a fecundação in vitro e cultivo in vitro, sendo, ao final desse processo, avaliada a taxa de estruturas clivadas. Em relação à taxa de expansão das células do cumulus, o grupo CON apresentou maiores taxas, quando comparado aos grupos adicionados de eugenol. Avaliando os oócitos com células do cumulus totalmente expandidas, somente o grupo EU40, apresentou-se menor, quando comparado ao restante dos grupos de tratamento. Já observando os oócitos com células do cumulus parcialmente expandidas, o grupo EU40, apresentou maiores percentuais de expansão, quando comparado aos demais tratamentos. Sobre a presença do primeiro corpúsculo polar nos oócitos, observou-se que não houve diferença estatística entre os grupos de tratamentos. E, por último, em relação às estruturas clivadas, os grupos CON e EU10 apresentaram as maiores taxas, quando comparado aos grupos EU20 e EU40. Dessa maneira, foi possível concluir que a utilização de 10 µM de eugenol na MIV de oócitos ovinos apresentou ser a melhor concentração dessa substância.SciELO PreprintsSciELO PreprintsSciELO Preprints2023-12-22info:eu-repo/semantics/preprintinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://preprints.scielo.org/index.php/scielo/preprint/view/775510.1590/SciELOPreprints.7755porhttps://preprints.scielo.org/index.php/scielo/article/view/7755/14494Copyright (c) 2023 Sara Rodrigues Pereira dos Santos, Brenna Maria Silva de Souza, Bruna Moura Silva, Damaris Raquel Pires dos Santos, Érika Karoline de Oliveira Aureliano, Andreza Mayara Carneiro Lima, Luana Kealy Pimentel de Oliveira, Sueli de Oliveira Lima, Ailton Batista Pereira , Isabela Maria Lopes, Beatriz Cavalcanti de Freitas, Thais Thatiane dos Santos Souza, Pedro Humberto Félix de Sousa, Mabel Freitas Cordeiro, Edilson Soares Lopes Júniorhttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessSantos, Sara Rodrigues Pereira dosSouza, Brenna Maria Silva deSilva, Bruna MouraSantos, Damaris Raquel Pires dosAureliano, Érika Karoline de OliveiraLima, Andreza Mayara CarneiroOliveira, Luana Kealy Pimentel deLima, Sueli de OliveiraPereira , Ailton BatistaLopes, Isabela MariaFreitas, Beatriz Cavalcanti deSouza, Thais Thatiane dos SantosSousa, Pedro Humberto Félix deCordeiro, Mabel FreitasLopes Júnior, Edilson Soaresreponame:SciELO Preprintsinstname:Scientific Electronic Library Online (SCIELO)instacron:SCI2023-12-20T00:07:22Zoai:ops.preprints.scielo.org:preprint/7755Servidor de preprintshttps://preprints.scielo.org/index.php/scieloONGhttps://preprints.scielo.org/index.php/scielo/oaiscielo.submission@scielo.orgopendoar:2023-12-20T00:07:22SciELO Preprints - Scientific Electronic Library Online (SCIELO)false |
dc.title.none.fl_str_mv |
Initial evaluations of the use of eugenol as an antioxidant in the in vitro maturation of ovine oocytes Evaluaciones iniciales del uso del eugenol como antioxidante en la maduración in vitro de ovocitos ovinos Avaliações iniciais do uso do eugenol como antioxidante na maturação in vitro de oócitos ovinos |
title |
Initial evaluations of the use of eugenol as an antioxidant in the in vitro maturation of ovine oocytes |
spellingShingle |
Initial evaluations of the use of eugenol as an antioxidant in the in vitro maturation of ovine oocytes Santos, Sara Rodrigues Pereira dos FIV MIV ovelha PIV zigoto IVF IVM IVP sheep zygote cigoto FIV MIV oveja PIV |
title_short |
Initial evaluations of the use of eugenol as an antioxidant in the in vitro maturation of ovine oocytes |
title_full |
Initial evaluations of the use of eugenol as an antioxidant in the in vitro maturation of ovine oocytes |
title_fullStr |
Initial evaluations of the use of eugenol as an antioxidant in the in vitro maturation of ovine oocytes |
title_full_unstemmed |
Initial evaluations of the use of eugenol as an antioxidant in the in vitro maturation of ovine oocytes |
title_sort |
Initial evaluations of the use of eugenol as an antioxidant in the in vitro maturation of ovine oocytes |
author |
Santos, Sara Rodrigues Pereira dos |
author_facet |
Santos, Sara Rodrigues Pereira dos Souza, Brenna Maria Silva de Silva, Bruna Moura Santos, Damaris Raquel Pires dos Aureliano, Érika Karoline de Oliveira Lima, Andreza Mayara Carneiro Oliveira, Luana Kealy Pimentel de Lima, Sueli de Oliveira Pereira , Ailton Batista Lopes, Isabela Maria Freitas, Beatriz Cavalcanti de Souza, Thais Thatiane dos Santos Sousa, Pedro Humberto Félix de Cordeiro, Mabel Freitas Lopes Júnior, Edilson Soares |
author_role |
author |
author2 |
Souza, Brenna Maria Silva de Silva, Bruna Moura Santos, Damaris Raquel Pires dos Aureliano, Érika Karoline de Oliveira Lima, Andreza Mayara Carneiro Oliveira, Luana Kealy Pimentel de Lima, Sueli de Oliveira Pereira , Ailton Batista Lopes, Isabela Maria Freitas, Beatriz Cavalcanti de Souza, Thais Thatiane dos Santos Sousa, Pedro Humberto Félix de Cordeiro, Mabel Freitas Lopes Júnior, Edilson Soares |
author2_role |
author author author author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Santos, Sara Rodrigues Pereira dos Souza, Brenna Maria Silva de Silva, Bruna Moura Santos, Damaris Raquel Pires dos Aureliano, Érika Karoline de Oliveira Lima, Andreza Mayara Carneiro Oliveira, Luana Kealy Pimentel de Lima, Sueli de Oliveira Pereira , Ailton Batista Lopes, Isabela Maria Freitas, Beatriz Cavalcanti de Souza, Thais Thatiane dos Santos Sousa, Pedro Humberto Félix de Cordeiro, Mabel Freitas Lopes Júnior, Edilson Soares |
dc.subject.por.fl_str_mv |
FIV MIV ovelha PIV zigoto IVF IVM IVP sheep zygote cigoto FIV MIV oveja PIV |
topic |
FIV MIV ovelha PIV zigoto IVF IVM IVP sheep zygote cigoto FIV MIV oveja PIV |
description |
The aim of this study was to assess the effect of eugenol on the in vitro production of ovine embryos. Oocytes were aspirated and taken for in vitro maturation (IVM), where they were divided into 4 groups: the CON (control), in which the oocytes were immersed in medium without an antioxidant; in the EU10, EU20, EU40 groups, the same medium as in the COM group was used, supplemented with 10, 20, and 40 µM of eugenol, respectively. The degree of expansion of cumulus cells was evaluated. Subsequently, the oocytes underwent in vitro fertilization and in vitro culture, and at the end of this process, the rate of cleaved structures was assessed. Regarding the expansion rate of cumulus cells, the CON group exhibited higher rates compared to the groups supplemented with eugenol. Evaluating oocytes with fully expanded cumulus cells, only the EU40 group showed a lower rate when compared to the rest of the treatment groups. Whereas observing oocytes with partially expanded cumulus cells, the EU40 group showed higher expansion percentages compared to the other treatments. Concerning the presence of the first polar body in the oocytes, it was observed that there was no statistical difference between the treatment groups. Lastly, concerning the cleaved structures, the CON and EU10 groups showed the highest rates compared to the EU20 and EU40 groups. Thus, it was possible to conclude that the use of 10 µM of eugenol in the IVM of ovine oocytes proved to be the optimal concentration of this substance. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-12-22 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/preprint info:eu-repo/semantics/publishedVersion |
format |
preprint |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://preprints.scielo.org/index.php/scielo/preprint/view/7755 10.1590/SciELOPreprints.7755 |
url |
https://preprints.scielo.org/index.php/scielo/preprint/view/7755 |
identifier_str_mv |
10.1590/SciELOPreprints.7755 |
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por |
language |
por |
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https://preprints.scielo.org/index.php/scielo/article/view/7755/14494 |
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https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
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https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
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application/pdf |
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SciELO Preprints SciELO Preprints SciELO Preprints |
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SciELO Preprints SciELO Preprints SciELO Preprints |
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Scientific Electronic Library Online (SCIELO) |
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SciELO Preprints - Scientific Electronic Library Online (SCIELO) |
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