Initial evaluations of quercetin on the in vitro maturation of ovine oocytes
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2023 |
| Outros Autores: | , , , , , , , , , , , , , |
| Tipo de documento: | preprint |
| Idioma: | por |
| Título da fonte: | SciELO Preprints |
| Texto Completo: | https://preprints.scielo.org/index.php/scielo/preprint/view/7759 |
Resumo: | The aim of this study was to evaluate the effect of quercetin on the in vitro maturation (IVM) of oocytes and the subsequent in vitro fertilization (IVF) of ovine embryos. For this purpose, oocytes were collected and divided into the following treatment groups: control group, containing CON medium (control); and groups Q2, Q4, and Q8, with control medium supplemented with 2, 4, and 8 µM of quercetin, respectively. After IVM, the degree of cumulus cells expansion, presence of the first polar body (PB), and the other part of the oocytes proceeded to in vitro fertilization (IVF), where oocytes were incubated with spermatozoa for a period of 20 hours. Subsequently, the presumptive zygotes underwent in vitro culture (IVC) for 48 hours, and at the end of the process, the rate of cleaved structures was evaluated. The results were expressed in percentage and compared using the Chi-square test, with a significant difference considered when P<0.05. Evaluating the expansion of cumulus cells, the CON and Q2 groups showed the best expansion rates compared to the Q4 and Q8 groups. Regarding the presence of the 1st PB, it was observed that only the Q8 group exhibited lower rates compared to the control group. When evaluating the rate of cleaved structures, it was observed that the Q2 group showed a higher number compared to the Q8 group. Quercetin does not influence the maturation of ovine oocytes. |
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Initial evaluations of quercetin on the in vitro maturation of ovine oocytesEvaluaciones iniciales de la quercetina en la maduración in vitro de ovocitos ovinosAvaliações iniciais da quercetina na maturação in vitro de oócitos ovinosAntioxidanteFIVMIVovelhaAntioxidantIVFIVMsheepAntioxidanteFIVMIVovejaThe aim of this study was to evaluate the effect of quercetin on the in vitro maturation (IVM) of oocytes and the subsequent in vitro fertilization (IVF) of ovine embryos. For this purpose, oocytes were collected and divided into the following treatment groups: control group, containing CON medium (control); and groups Q2, Q4, and Q8, with control medium supplemented with 2, 4, and 8 µM of quercetin, respectively. After IVM, the degree of cumulus cells expansion, presence of the first polar body (PB), and the other part of the oocytes proceeded to in vitro fertilization (IVF), where oocytes were incubated with spermatozoa for a period of 20 hours. Subsequently, the presumptive zygotes underwent in vitro culture (IVC) for 48 hours, and at the end of the process, the rate of cleaved structures was evaluated. The results were expressed in percentage and compared using the Chi-square test, with a significant difference considered when P<0.05. Evaluating the expansion of cumulus cells, the CON and Q2 groups showed the best expansion rates compared to the Q4 and Q8 groups. Regarding the presence of the 1st PB, it was observed that only the Q8 group exhibited lower rates compared to the control group. When evaluating the rate of cleaved structures, it was observed that the Q2 group showed a higher number compared to the Q8 group. Quercetin does not influence the maturation of ovine oocytes.El objetivo del presente estudio fue evaluar el efecto de la quercetina en la maduración in vitro (MIV) de ovocitos y la posterior fertilización in vitro (FIV) de embriones ovinos. Para ello, se recolectaron y seleccionaron ovocitos divididos en los siguientes grupos de tratamiento: grupo control, que contenía medio CON (control); y los grupos Q2, Q4 y Q8, con medio control al que se le añadieron 2, 4 y 8 µM de quercetina, respectivamente. Tras la MIV se evaluó el grado de expansión de las células del cúmulo, la presencia del primer corpúsculo polar (CP) y la otra parte de los ovocitos se utilizó para la fecundación in vitro (FIV), donde los ovocitos fueron incubados con espermatozoides durante un período de 20 horas. Posteriormente, los presuntos cigotos pasaron a cultivo in vitro (CIV) durante 48 horas y, al final del proceso, se evaluó la tasa de estructuras clivadas. Los resultados se expresaron en porcentaje y se compararon utilizando la prueba de Chi-cuadrado, considerando una diferencia significativa cuando P<0,05. Al evaluar la expansión de las células del cúmulo, los grupos CON y Q2 mostraron las mejores tasas de expansión en comparación con los grupos Q4 y Q8. En cuanto a la presencia del 1er CP, se observó que solo el grupo Q8 mostró tasas más bajas en comparación con el grupo control. Al evaluar la tasa de estructuras clivadas, se observó que el grupo Q2 mostró un número mayor en comparación con el grupo Q8. La quercetina no influye en la maduración de ovocitos ovinos.O objetivo do presente estudo foi avaliar o efeito da quercetina na MIV de oócitos e na subsequente PIV de embriões ovinos. Para isso, foram colhidos e selecionados oócitos divididos nos seguintes grupos de tratamento: grupo controle, contendo meio CON (controle); e grupos Q2, Q4 e Q8, com meio controle, acrescido de 2, 4 e 8 µM de quercetina, respectivamente. Após a MIV foi avaliado o grau de expansão das células do cumulus, presença do primeiro corpúsculo polar (CP) e a outra parte dos oócitos seguiu para a fecundação in vitro (FIV), onde os oócitos foram incubados com os espermatozoides, por um período de 20 h. Em seguida, os presumíveis zigotos seguiram para o cultivo in vitro (CIV), por um período de 48 h e, ao final do processo, foi avaliada a taxa de estruturas clivadas. Os resultados foram expressos em porcentagem e comparados, usando o Teste do Qui-quadrado e foi considerada diferença significativa quando P<0,05. Avaliando a expansão das células do cumulus, os grupos CON e Q2 apresentaram as melhores taxas de expansão, quando comparadas aos grupos Q4 e Q8. Quanto à presença do 1º CP, foi possível observar que somente o grupo Q8 apresentou taxas menores, quando comparado ao grupo controle. Já avaliando a taxa de estruturas clivadas, foi possível observar que o grupo Q2 apresentou maior número quando comparado ao grupo Q8. A quercetina não influencia na maturação de oócitos ovinos.SciELO PreprintsSciELO PreprintsSciELO Preprints2023-12-22info:eu-repo/semantics/preprintinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://preprints.scielo.org/index.php/scielo/preprint/view/775910.1590/SciELOPreprints.7759porhttps://preprints.scielo.org/index.php/scielo/article/view/7759/14498Copyright (c) 2023 Bruna Moura Silva, Sara Rodrigues Pereira dos Santos, Brenna Maria Silva de Souza, Damaris Raquel Pires dos Santos, Érika Karoline de Oliveira Aureliano, Andreza Mayara Carneiro Lima, Luana Kealy Pimentel de Oliveira, Sueli de Oliveira Lima, Ailton Batista Pereira , Isabela Maria Lopes, Beatriz Cavalcanti de Freitas, Thais Thatiane dos Santos Souza, Pedro Humberto Félix de Sousa, Mabel Freitas Cordeiro, Edilson Soares Lopes Júniorhttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessSilva, Bruna MouraSantos, Sara Rodrigues Pereira dosSouza, Brenna Maria Silva deSantos, Damaris Raquel Pires dosAureliano, Érika Karoline de OliveiraLima, Andreza Mayara CarneiroOliveira, Luana Kealy Pimentel deLima, Sueli de OliveiraPereira , Ailton BatistaLopes, Isabela MariaFreitas, Beatriz Cavalcanti deSouza, Thais Thatiane dos SantosSousa, Pedro Humberto Félix deCordeiro, Mabel FreitasLopes Júnior, Edilson Soaresreponame:SciELO Preprintsinstname:Scientific Electronic Library Online (SCIELO)instacron:SCI2023-12-20T02:30:51Zoai:ops.preprints.scielo.org:preprint/7759Servidor de preprintshttps://preprints.scielo.org/index.php/scieloONGhttps://preprints.scielo.org/index.php/scielo/oaiscielo.submission@scielo.orgopendoar:2023-12-20T02:30:51SciELO Preprints - Scientific Electronic Library Online (SCIELO)false |
| dc.title.none.fl_str_mv |
Initial evaluations of quercetin on the in vitro maturation of ovine oocytes Evaluaciones iniciales de la quercetina en la maduración in vitro de ovocitos ovinos Avaliações iniciais da quercetina na maturação in vitro de oócitos ovinos |
| title |
Initial evaluations of quercetin on the in vitro maturation of ovine oocytes |
| spellingShingle |
Initial evaluations of quercetin on the in vitro maturation of ovine oocytes Silva, Bruna Moura Antioxidante FIV MIV ovelha Antioxidant IVF IVM sheep Antioxidante FIV MIV oveja |
| title_short |
Initial evaluations of quercetin on the in vitro maturation of ovine oocytes |
| title_full |
Initial evaluations of quercetin on the in vitro maturation of ovine oocytes |
| title_fullStr |
Initial evaluations of quercetin on the in vitro maturation of ovine oocytes |
| title_full_unstemmed |
Initial evaluations of quercetin on the in vitro maturation of ovine oocytes |
| title_sort |
Initial evaluations of quercetin on the in vitro maturation of ovine oocytes |
| author |
Silva, Bruna Moura |
| author_facet |
Silva, Bruna Moura Santos, Sara Rodrigues Pereira dos Souza, Brenna Maria Silva de Santos, Damaris Raquel Pires dos Aureliano, Érika Karoline de Oliveira Lima, Andreza Mayara Carneiro Oliveira, Luana Kealy Pimentel de Lima, Sueli de Oliveira Pereira , Ailton Batista Lopes, Isabela Maria Freitas, Beatriz Cavalcanti de Souza, Thais Thatiane dos Santos Sousa, Pedro Humberto Félix de Cordeiro, Mabel Freitas Lopes Júnior, Edilson Soares |
| author_role |
author |
| author2 |
Santos, Sara Rodrigues Pereira dos Souza, Brenna Maria Silva de Santos, Damaris Raquel Pires dos Aureliano, Érika Karoline de Oliveira Lima, Andreza Mayara Carneiro Oliveira, Luana Kealy Pimentel de Lima, Sueli de Oliveira Pereira , Ailton Batista Lopes, Isabela Maria Freitas, Beatriz Cavalcanti de Souza, Thais Thatiane dos Santos Sousa, Pedro Humberto Félix de Cordeiro, Mabel Freitas Lopes Júnior, Edilson Soares |
| author2_role |
author author author author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Silva, Bruna Moura Santos, Sara Rodrigues Pereira dos Souza, Brenna Maria Silva de Santos, Damaris Raquel Pires dos Aureliano, Érika Karoline de Oliveira Lima, Andreza Mayara Carneiro Oliveira, Luana Kealy Pimentel de Lima, Sueli de Oliveira Pereira , Ailton Batista Lopes, Isabela Maria Freitas, Beatriz Cavalcanti de Souza, Thais Thatiane dos Santos Sousa, Pedro Humberto Félix de Cordeiro, Mabel Freitas Lopes Júnior, Edilson Soares |
| dc.subject.por.fl_str_mv |
Antioxidante FIV MIV ovelha Antioxidant IVF IVM sheep Antioxidante FIV MIV oveja |
| topic |
Antioxidante FIV MIV ovelha Antioxidant IVF IVM sheep Antioxidante FIV MIV oveja |
| description |
The aim of this study was to evaluate the effect of quercetin on the in vitro maturation (IVM) of oocytes and the subsequent in vitro fertilization (IVF) of ovine embryos. For this purpose, oocytes were collected and divided into the following treatment groups: control group, containing CON medium (control); and groups Q2, Q4, and Q8, with control medium supplemented with 2, 4, and 8 µM of quercetin, respectively. After IVM, the degree of cumulus cells expansion, presence of the first polar body (PB), and the other part of the oocytes proceeded to in vitro fertilization (IVF), where oocytes were incubated with spermatozoa for a period of 20 hours. Subsequently, the presumptive zygotes underwent in vitro culture (IVC) for 48 hours, and at the end of the process, the rate of cleaved structures was evaluated. The results were expressed in percentage and compared using the Chi-square test, with a significant difference considered when P<0.05. Evaluating the expansion of cumulus cells, the CON and Q2 groups showed the best expansion rates compared to the Q4 and Q8 groups. Regarding the presence of the 1st PB, it was observed that only the Q8 group exhibited lower rates compared to the control group. When evaluating the rate of cleaved structures, it was observed that the Q2 group showed a higher number compared to the Q8 group. Quercetin does not influence the maturation of ovine oocytes. |
| publishDate |
2023 |
| dc.date.none.fl_str_mv |
2023-12-22 |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/preprint info:eu-repo/semantics/publishedVersion |
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preprint |
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publishedVersion |
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https://preprints.scielo.org/index.php/scielo/preprint/view/7759 10.1590/SciELOPreprints.7759 |
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https://preprints.scielo.org/index.php/scielo/preprint/view/7759 |
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10.1590/SciELOPreprints.7759 |
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por |
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por |
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https://preprints.scielo.org/index.php/scielo/article/view/7759/14498 |
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openAccess |
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