PLANT EXTRACTS (Nicotiana glauca and Chrysanthemum vulgaris) IN A VACCINE TO BOVINE HERPESVIRUS TYPE 5 (BHV-5)

Detalhes bibliográficos
Autor(a) principal: FELDENS, OTTO
Data de Publicação: 2000
Outros Autores: VIDOR, TELMO, HÜBNER, SILVIA DE OLIVEIRA, HALFEN, DANIZA COELHO
Tipo de documento: Artigo
Idioma: por
Título da fonte: Pesquisa Agropecuária Gaúcha (Online)
Texto Completo: http://revistapag.agricultura.rs.gov.br/ojs/index.php/revistapag/article/view/470
Resumo: The effect of Nicotiana glauca and Chrysanthemum vulgaris leave extracts on the humoral response in cattle vaccinated with an inactivated vaccine to bovine Herpesvirus type 5 (BHV-5) were examined. Two inactivated oil-adjuvanted BHV-5 vaccines were prepared with an infectious titre (pre-inactivation) of 10 84DICC/ ml, inactivated with binary ethylenimine (BEI) and emulsified in oil adjuvant. Nicotiana glauca and Chrysanthemum vulgaris extracts were added . (5mg of each extract per dose) as a coadjuvant to one of the vaccines (vaccine B). Two groups of nineteen calves were vaccinated with 3 doses (3 ml) at four week intervals between doses. Neutralizing antibody leveis in sera were determined at different time intervals after vaccination. Thirty days after the administration of the third dose the mean neutralizing antibody titre was 96 for vaccine A and 80 for vaccine B. There was no significant difference between the antibody titres induced by the two vaccines (p>0.05). These results indicate that extracts of these plants had no adjuvant effect on the inactivated BHV-5 vaccine .
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spelling PLANT EXTRACTS (Nicotiana glauca and Chrysanthemum vulgaris) IN A VACCINE TO BOVINE HERPESVIRUS TYPE 5 (BHV-5)EXTRATOS VEGETAIS (Nicotiana glauca e Chrysanthemum vulgaris) EM UMA VACINA CONTRA O HERPESVÍRUS BOVINO TIPO 5 (BHV-5) Bovine herpesvirus, Bovine encephalitis virus, BHV-5, immunostimulants, vaccine.Herpesvírus bovino, Vírus da encefalite bovina, BHV-5, imunoestimulantes, vacina.The effect of Nicotiana glauca and Chrysanthemum vulgaris leave extracts on the humoral response in cattle vaccinated with an inactivated vaccine to bovine Herpesvirus type 5 (BHV-5) were examined. Two inactivated oil-adjuvanted BHV-5 vaccines were prepared with an infectious titre (pre-inactivation) of 10 84DICC/ ml, inactivated with binary ethylenimine (BEI) and emulsified in oil adjuvant. Nicotiana glauca and Chrysanthemum vulgaris extracts were added . (5mg of each extract per dose) as a coadjuvant to one of the vaccines (vaccine B). Two groups of nineteen calves were vaccinated with 3 doses (3 ml) at four week intervals between doses. Neutralizing antibody leveis in sera were determined at different time intervals after vaccination. Thirty days after the administration of the third dose the mean neutralizing antibody titre was 96 for vaccine A and 80 for vaccine B. There was no significant difference between the antibody titres induced by the two vaccines (p>0.05). These results indicate that extracts of these plants had no adjuvant effect on the inactivated BHV-5 vaccine . Este trabalho analisa o efeito da adição do extrato das folhas de Nicotiana glauca e Chrysanthemum vulgaris em vacina inativada contra Herpesvírus bovino tipo 5 (BHV-5) sobre a resposta imune humoral em bovinos vacinados. Duas vacinas para BHV-5 inativadas com etileno mina binária e emulsificadas com adjuvante oleoso foram produzidas a partir de uma suspensão viral com título de 10 8 . 6 DICC50/ml. Uma das vacinas (vacina 13) teve a incorporação de extrato bruto das plantas Nicotiana glauca e Chrysanthemum vulgaris na dose de 5mg de extrato de cada planta por dose. As vacinas foram testadas em dois grupos de 19 bovinos, vacinados com 3 doses (3m1) de cada vacina com intervalo mensal. Os títulos de anticorpos neutralizantes foram determinados em diferentes intervalos após a vacinação através do teste de soroneutralização. Trinta dias após a terceira dose vacinal a vacina A induziu título médio de anticorpos de 96 e a vacina B de 80. Na análise estatística não houve diferença significativa entre os títulos de anticorpos induzidos pelas duas vacinas (p>0,05). Os resultados indicaram que a adição dos extratos destas plantas não influenciou a resposta imune humoral nesta vacina. Departamento de Diagnóstico e Pesquisa Agropecuária. Secretaria de Agricultura, Pecuária e Desenvolvimento Rural2000-12-29info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://revistapag.agricultura.rs.gov.br/ojs/index.php/revistapag/article/view/470Pesquisa Agropecuária Gaúcha; Vol 6 No 2 (2000): PESQ. AGROP. GAÚCHA; 309-315Pesquisa Agropecuária Gaúcha; v. 6 n. 2 (2000): PESQ. AGROP. GAÚCHA; 309-3152595-76860104-9070reponame:Pesquisa Agropecuária Gaúcha (Online)instname:Secretaria de Agricultura, Pecuária e Desenvolvimento Rural do Rio Grande do Sulinstacron:SEAPDRporhttp://revistapag.agricultura.rs.gov.br/ojs/index.php/revistapag/article/view/470/434FELDENS, OTTO VIDOR, TELMO HÜBNER, SILVIA DE OLIVEIRA HALFEN, DANIZA COELHO info:eu-repo/semantics/openAccess2020-08-08T21:33:15Zoai:ojs.revistapag.agricultura.rs.gov.br:article/470Revistahttp://revistapag.agricultura.rs.gov.br/ojs/index.php/revistapagPUBhttp://revistapag.agricultura.rs.gov.br/ojs/index.php/revistapag/oaipag@agricultura.rs.gov.br||2595-76860104-9070opendoar:2020-08-08T21:33:15Pesquisa Agropecuária Gaúcha (Online) - Secretaria de Agricultura, Pecuária e Desenvolvimento Rural do Rio Grande do Sulfalse
dc.title.none.fl_str_mv PLANT EXTRACTS (Nicotiana glauca and Chrysanthemum vulgaris) IN A VACCINE TO BOVINE HERPESVIRUS TYPE 5 (BHV-5)
EXTRATOS VEGETAIS (Nicotiana glauca e Chrysanthemum vulgaris) EM UMA VACINA CONTRA O HERPESVÍRUS BOVINO TIPO 5 (BHV-5)
title PLANT EXTRACTS (Nicotiana glauca and Chrysanthemum vulgaris) IN A VACCINE TO BOVINE HERPESVIRUS TYPE 5 (BHV-5)
spellingShingle PLANT EXTRACTS (Nicotiana glauca and Chrysanthemum vulgaris) IN A VACCINE TO BOVINE HERPESVIRUS TYPE 5 (BHV-5)
FELDENS, OTTO
Bovine herpesvirus, Bovine encephalitis virus, BHV-5, immunostimulants, vaccine.
Herpesvírus bovino, Vírus da encefalite bovina, BHV-5, imunoestimulantes, vacina.
title_short PLANT EXTRACTS (Nicotiana glauca and Chrysanthemum vulgaris) IN A VACCINE TO BOVINE HERPESVIRUS TYPE 5 (BHV-5)
title_full PLANT EXTRACTS (Nicotiana glauca and Chrysanthemum vulgaris) IN A VACCINE TO BOVINE HERPESVIRUS TYPE 5 (BHV-5)
title_fullStr PLANT EXTRACTS (Nicotiana glauca and Chrysanthemum vulgaris) IN A VACCINE TO BOVINE HERPESVIRUS TYPE 5 (BHV-5)
title_full_unstemmed PLANT EXTRACTS (Nicotiana glauca and Chrysanthemum vulgaris) IN A VACCINE TO BOVINE HERPESVIRUS TYPE 5 (BHV-5)
title_sort PLANT EXTRACTS (Nicotiana glauca and Chrysanthemum vulgaris) IN A VACCINE TO BOVINE HERPESVIRUS TYPE 5 (BHV-5)
author FELDENS, OTTO
author_facet FELDENS, OTTO
VIDOR, TELMO
HÜBNER, SILVIA DE OLIVEIRA
HALFEN, DANIZA COELHO
author_role author
author2 VIDOR, TELMO
HÜBNER, SILVIA DE OLIVEIRA
HALFEN, DANIZA COELHO
author2_role author
author
author
dc.contributor.author.fl_str_mv FELDENS, OTTO
VIDOR, TELMO
HÜBNER, SILVIA DE OLIVEIRA
HALFEN, DANIZA COELHO
dc.subject.por.fl_str_mv Bovine herpesvirus, Bovine encephalitis virus, BHV-5, immunostimulants, vaccine.
Herpesvírus bovino, Vírus da encefalite bovina, BHV-5, imunoestimulantes, vacina.
topic Bovine herpesvirus, Bovine encephalitis virus, BHV-5, immunostimulants, vaccine.
Herpesvírus bovino, Vírus da encefalite bovina, BHV-5, imunoestimulantes, vacina.
description The effect of Nicotiana glauca and Chrysanthemum vulgaris leave extracts on the humoral response in cattle vaccinated with an inactivated vaccine to bovine Herpesvirus type 5 (BHV-5) were examined. Two inactivated oil-adjuvanted BHV-5 vaccines were prepared with an infectious titre (pre-inactivation) of 10 84DICC/ ml, inactivated with binary ethylenimine (BEI) and emulsified in oil adjuvant. Nicotiana glauca and Chrysanthemum vulgaris extracts were added . (5mg of each extract per dose) as a coadjuvant to one of the vaccines (vaccine B). Two groups of nineteen calves were vaccinated with 3 doses (3 ml) at four week intervals between doses. Neutralizing antibody leveis in sera were determined at different time intervals after vaccination. Thirty days after the administration of the third dose the mean neutralizing antibody titre was 96 for vaccine A and 80 for vaccine B. There was no significant difference between the antibody titres induced by the two vaccines (p>0.05). These results indicate that extracts of these plants had no adjuvant effect on the inactivated BHV-5 vaccine .
publishDate 2000
dc.date.none.fl_str_mv 2000-12-29
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://revistapag.agricultura.rs.gov.br/ojs/index.php/revistapag/article/view/470
url http://revistapag.agricultura.rs.gov.br/ojs/index.php/revistapag/article/view/470
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv http://revistapag.agricultura.rs.gov.br/ojs/index.php/revistapag/article/view/470/434
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Departamento de Diagnóstico e Pesquisa Agropecuária. Secretaria de Agricultura, Pecuária e Desenvolvimento Rural
publisher.none.fl_str_mv Departamento de Diagnóstico e Pesquisa Agropecuária. Secretaria de Agricultura, Pecuária e Desenvolvimento Rural
dc.source.none.fl_str_mv Pesquisa Agropecuária Gaúcha; Vol 6 No 2 (2000): PESQ. AGROP. GAÚCHA; 309-315
Pesquisa Agropecuária Gaúcha; v. 6 n. 2 (2000): PESQ. AGROP. GAÚCHA; 309-315
2595-7686
0104-9070
reponame:Pesquisa Agropecuária Gaúcha (Online)
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instname_str Secretaria de Agricultura, Pecuária e Desenvolvimento Rural do Rio Grande do Sul
instacron_str SEAPDR
institution SEAPDR
reponame_str Pesquisa Agropecuária Gaúcha (Online)
collection Pesquisa Agropecuária Gaúcha (Online)
repository.name.fl_str_mv Pesquisa Agropecuária Gaúcha (Online) - Secretaria de Agricultura, Pecuária e Desenvolvimento Rural do Rio Grande do Sul
repository.mail.fl_str_mv pag@agricultura.rs.gov.br||
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