EVALUATION OF AN INDIRECT ELISA FOR DETECTION AND TYPING OF FOOT AND MOUTH DISEASE VIRUS

Detalhes bibliográficos
Autor(a) principal: PIRES PRADO, JOSÉ ANTONIO
Data de Publicação: 1996
Outros Autores: FREITAS TEIXEIRA, JOÃO CARLOS, RECKZIEGEL, PAULO ESTANISLÁO, PETZHOLD, SYLIO ALFREDO
Tipo de documento: Artigo
Idioma: por
Título da fonte: Pesquisa Agropecuária Gaúcha (Online)
Texto Completo: http://revistapag.agricultura.rs.gov.br/ojs/index.php/revistapag/article/view/605
Resumo: A Indirect enzyme-linked immunosorbent assay (ELISA) kit was used for diagnosis of foot and mouth disease virus (FMDV) typeS 01, A24, C3 which occured in Rio Grande do Sul State, Southern Brazil during 1984-1993. The samples were randomly selected and tested by. Elisa, Complement Fixation Test (CFT) and in tissue ctilture. Out of 106 samples 78 (73:5%) were positive by Elisa and 39(36.8 %) were found positive in CFT, when original suspensions were used. Once_those samples were inoculated onto tissue culture both tests gave similar results, although Elisa picked tip more positive samples during the lst passage in tissue culture. The negative samples (16) included in this study were negative in all tests. The Elisa was more sensitive than and as speci fie as CFT. Elisa and tissue culture together were shown to be a better system for detection of Foot and Mouth Disease (FMD) antigen than CFT.
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spelling EVALUATION OF AN INDIRECT ELISA FOR DETECTION AND TYPING OF FOOT AND MOUTH DISEASE VIRUS AVALIAÇÃO DE UM TESTE DE ELISA INDIRETO PARA DETECTAR E TIPIFICAR VIRUS DA FEBRE AFTOSA Foot and mouth disease.Indirect Elisa.Complement fixation.Tissue culture.Febre aftosa.Elisa indireto.Fixação de complemento.Cultura de tecidos.A Indirect enzyme-linked immunosorbent assay (ELISA) kit was used for diagnosis of foot and mouth disease virus (FMDV) typeS 01, A24, C3 which occured in Rio Grande do Sul State, Southern Brazil during 1984-1993. The samples were randomly selected and tested by. Elisa, Complement Fixation Test (CFT) and in tissue ctilture. Out of 106 samples 78 (73:5%) were positive by Elisa and 39(36.8 %) were found positive in CFT, when original suspensions were used. Once_those samples were inoculated onto tissue culture both tests gave similar results, although Elisa picked tip more positive samples during the lst passage in tissue culture. The negative samples (16) included in this study were negative in all tests. The Elisa was more sensitive than and as speci fie as CFT. Elisa and tissue culture together were shown to be a better system for detection of Foot and Mouth Disease (FMD) antigen than CFT.Um kit de elisa indireto foi usado para diagnosticar o virus da febre aftosa para os tipos 01, A24, C3 incidentes no Estado do Rio Grande do Sul no período 1984 - 1993. As amostras foram selecionadas ao acaso e testadas por elisa, fixação do complemento(FC) e em cultura de tecido.De um total de 106 amostras de suspensões originais, 78(73,5%) foram positivas em elisa e 39(36,8%) foram positivas por fixação do complemento. Quando estas amostras foram inoculadas em culturas de células, ambos os testes tiveram idêntica performance, embora o teste de elisa tenha sido capaz de detectar mais amostras positivas que o teste de fixação de complemento durante a l' passagem em cultura de tecido. Todas as amostras negativas(16) incluídas no experimento se mostraram negativas em todos os testes e durante toda a investigação. O teste de elisa foi mais sensível que FC; elisa e FC tiveram a mesma especificidade. Elisa e cultura de tecidos se mostraram o melhor sistema paradetectar o antígeno de febre aftosa que o teste de FC. Departamento de Diagnóstico e Pesquisa Agropecuária. Secretaria de Agricultura, Pecuária e Desenvolvimento Rural1996-01-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://revistapag.agricultura.rs.gov.br/ojs/index.php/revistapag/article/view/605Pesquisa Agropecuária Gaúcha; Vol 2 No 1 (1996): PESQ. AGROP. GAÚCHA; 107-109Pesquisa Agropecuária Gaúcha; v. 2 n. 1 (1996): PESQ. AGROP. GAÚCHA; 107-1092595-76860104-9070reponame:Pesquisa Agropecuária Gaúcha (Online)instname:Secretaria de Agricultura, Pecuária e Desenvolvimento Rural do Rio Grande do Sulinstacron:SEAPDRporhttp://revistapag.agricultura.rs.gov.br/ojs/index.php/revistapag/article/view/605/556PIRES PRADO, JOSÉ ANTONIO FREITAS TEIXEIRA, JOÃO CARLOS RECKZIEGEL, PAULO ESTANISLÁO PETZHOLD, SYLIO ALFREDO info:eu-repo/semantics/openAccess2024-04-22T12:01:36Zoai:ojs.revistapag.agricultura.rs.gov.br:article/605Revistahttp://revistapag.agricultura.rs.gov.br/ojs/index.php/revistapagPUBhttp://revistapag.agricultura.rs.gov.br/ojs/index.php/revistapag/oaipag@agricultura.rs.gov.br||2595-76860104-9070opendoar:2024-04-22T12:01:36Pesquisa Agropecuária Gaúcha (Online) - Secretaria de Agricultura, Pecuária e Desenvolvimento Rural do Rio Grande do Sulfalse
dc.title.none.fl_str_mv EVALUATION OF AN INDIRECT ELISA FOR DETECTION AND TYPING OF FOOT AND MOUTH DISEASE VIRUS
AVALIAÇÃO DE UM TESTE DE ELISA INDIRETO PARA DETECTAR E TIPIFICAR VIRUS DA FEBRE AFTOSA
title EVALUATION OF AN INDIRECT ELISA FOR DETECTION AND TYPING OF FOOT AND MOUTH DISEASE VIRUS
spellingShingle EVALUATION OF AN INDIRECT ELISA FOR DETECTION AND TYPING OF FOOT AND MOUTH DISEASE VIRUS
PIRES PRADO, JOSÉ ANTONIO
Foot and mouth disease.
Indirect Elisa.
Complement fixation.
Tissue culture.
Febre aftosa.
Elisa indireto.
Fixação de complemento.
Cultura de tecidos.
title_short EVALUATION OF AN INDIRECT ELISA FOR DETECTION AND TYPING OF FOOT AND MOUTH DISEASE VIRUS
title_full EVALUATION OF AN INDIRECT ELISA FOR DETECTION AND TYPING OF FOOT AND MOUTH DISEASE VIRUS
title_fullStr EVALUATION OF AN INDIRECT ELISA FOR DETECTION AND TYPING OF FOOT AND MOUTH DISEASE VIRUS
title_full_unstemmed EVALUATION OF AN INDIRECT ELISA FOR DETECTION AND TYPING OF FOOT AND MOUTH DISEASE VIRUS
title_sort EVALUATION OF AN INDIRECT ELISA FOR DETECTION AND TYPING OF FOOT AND MOUTH DISEASE VIRUS
author PIRES PRADO, JOSÉ ANTONIO
author_facet PIRES PRADO, JOSÉ ANTONIO
FREITAS TEIXEIRA, JOÃO CARLOS
RECKZIEGEL, PAULO ESTANISLÁO
PETZHOLD, SYLIO ALFREDO
author_role author
author2 FREITAS TEIXEIRA, JOÃO CARLOS
RECKZIEGEL, PAULO ESTANISLÁO
PETZHOLD, SYLIO ALFREDO
author2_role author
author
author
dc.contributor.author.fl_str_mv PIRES PRADO, JOSÉ ANTONIO
FREITAS TEIXEIRA, JOÃO CARLOS
RECKZIEGEL, PAULO ESTANISLÁO
PETZHOLD, SYLIO ALFREDO
dc.subject.por.fl_str_mv Foot and mouth disease.
Indirect Elisa.
Complement fixation.
Tissue culture.
Febre aftosa.
Elisa indireto.
Fixação de complemento.
Cultura de tecidos.
topic Foot and mouth disease.
Indirect Elisa.
Complement fixation.
Tissue culture.
Febre aftosa.
Elisa indireto.
Fixação de complemento.
Cultura de tecidos.
description A Indirect enzyme-linked immunosorbent assay (ELISA) kit was used for diagnosis of foot and mouth disease virus (FMDV) typeS 01, A24, C3 which occured in Rio Grande do Sul State, Southern Brazil during 1984-1993. The samples were randomly selected and tested by. Elisa, Complement Fixation Test (CFT) and in tissue ctilture. Out of 106 samples 78 (73:5%) were positive by Elisa and 39(36.8 %) were found positive in CFT, when original suspensions were used. Once_those samples were inoculated onto tissue culture both tests gave similar results, although Elisa picked tip more positive samples during the lst passage in tissue culture. The negative samples (16) included in this study were negative in all tests. The Elisa was more sensitive than and as speci fie as CFT. Elisa and tissue culture together were shown to be a better system for detection of Foot and Mouth Disease (FMD) antigen than CFT.
publishDate 1996
dc.date.none.fl_str_mv 1996-01-08
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://revistapag.agricultura.rs.gov.br/ojs/index.php/revistapag/article/view/605
url http://revistapag.agricultura.rs.gov.br/ojs/index.php/revistapag/article/view/605
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv http://revistapag.agricultura.rs.gov.br/ojs/index.php/revistapag/article/view/605/556
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Departamento de Diagnóstico e Pesquisa Agropecuária. Secretaria de Agricultura, Pecuária e Desenvolvimento Rural
publisher.none.fl_str_mv Departamento de Diagnóstico e Pesquisa Agropecuária. Secretaria de Agricultura, Pecuária e Desenvolvimento Rural
dc.source.none.fl_str_mv Pesquisa Agropecuária Gaúcha; Vol 2 No 1 (1996): PESQ. AGROP. GAÚCHA; 107-109
Pesquisa Agropecuária Gaúcha; v. 2 n. 1 (1996): PESQ. AGROP. GAÚCHA; 107-109
2595-7686
0104-9070
reponame:Pesquisa Agropecuária Gaúcha (Online)
instname:Secretaria de Agricultura, Pecuária e Desenvolvimento Rural do Rio Grande do Sul
instacron:SEAPDR
instname_str Secretaria de Agricultura, Pecuária e Desenvolvimento Rural do Rio Grande do Sul
instacron_str SEAPDR
institution SEAPDR
reponame_str Pesquisa Agropecuária Gaúcha (Online)
collection Pesquisa Agropecuária Gaúcha (Online)
repository.name.fl_str_mv Pesquisa Agropecuária Gaúcha (Online) - Secretaria de Agricultura, Pecuária e Desenvolvimento Rural do Rio Grande do Sul
repository.mail.fl_str_mv pag@agricultura.rs.gov.br||
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