Predominante of Ralstonia solanacearum biovar 1 in vegetable crops under crop in Rio Grande do Sul State
Autor(a) principal: | |
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Data de Publicação: | 2007 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Pesquisa Agropecuária Gaúcha (Online) |
Texto Completo: | http://revistapag.agricultura.rs.gov.br/ojs/index.php/revistapag/article/view/268 |
Resumo: | In ordcr to verify the occurrence and distribution of biovars of Ralstonia solanacearum in vegetable crops under crop in the Statc of Rio Grande do Sul, Brazil. samples of tomato, eggplant and tobacco showing symptoms of bacterial wilt were collectcd in the spring of 2004, in 12 fanes from four citics: Maquiné, Caxias do Sul, Sobradinho and Santa Cruz do Sul. The identification of the bacterial species was carried out by the immunological test of ELISA and thc classification in biovar was based on the oxidative metabolism of alcohols and sugars and in the PCR fingerprinting. All thc 54 isolatcs were identificd as biovar 1. l-lowever, the amplification of DNA with the primas T3A and T5A resulted in a profile different from that expectcd for R. solanacearum strains, showing the amplification of an additional product with 650 bp. Dueto its dimane conditions and according to previous surveys in potato flelds, it was cstablished thc predominante of the biovar 2 of R. solanacearum. This study demonstrated that conditions of climate and soil in Rio Grande do Sul State pennit the occurrence of both biovars I and 2 and that the predominante of one or other is determined by the host spccics. |
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Predominante of Ralstonia solanacearum biovar 1 in vegetable crops under crop in Rio Grande do Sul State Predominância da biovar 1 de Ralstonia solanacearum em olerícolas cultivadas no Estado do Rio Grande do Sulepidemiology, bacterial wilt, biovar, tomato, eggplant, tobaccocpidemiologia, murcha bacteriana, biovar, tomate, berinjela, fumoIn ordcr to verify the occurrence and distribution of biovars of Ralstonia solanacearum in vegetable crops under crop in the Statc of Rio Grande do Sul, Brazil. samples of tomato, eggplant and tobacco showing symptoms of bacterial wilt were collectcd in the spring of 2004, in 12 fanes from four citics: Maquiné, Caxias do Sul, Sobradinho and Santa Cruz do Sul. The identification of the bacterial species was carried out by the immunological test of ELISA and thc classification in biovar was based on the oxidative metabolism of alcohols and sugars and in the PCR fingerprinting. All thc 54 isolatcs were identificd as biovar 1. l-lowever, the amplification of DNA with the primas T3A and T5A resulted in a profile different from that expectcd for R. solanacearum strains, showing the amplification of an additional product with 650 bp. Dueto its dimane conditions and according to previous surveys in potato flelds, it was cstablished thc predominante of the biovar 2 of R. solanacearum. This study demonstrated that conditions of climate and soil in Rio Grande do Sul State pennit the occurrence of both biovars I and 2 and that the predominante of one or other is determined by the host spccics. Com a finalidade de verificar a ocorrência e a distribuição de biovares de Ralstonia solanacearum em olericolas cultivadas no Estado do Rio Grande do Sul, plantas de tomate, berinjela e fumo com sintonias de murcha bacteriana foram coletadas, na primavera de 2004, em 12 lavouras de quatro municípios: Maquiné, Caxias do Sul, Sobradinho e Santa Cruz do Sul. A determinação da espécie foi feita com teste sorológico através de ELISA c a da biovar, com base no metabolismo oxidativo de açúcares e álcoois e por PCR. Todos os 54 isolados foram identificados como biovar I. Porém, a amplificação por PCR com os oligonucleolídeos iniciadores T3A e T5A resultaram em tipo diferente de perfil daqueles previstos para estirpes deste patógcno, com a amplificação de um produto de adicional de 650 pb. Devido às condições climáticas do Estado do Rio Grande do Sul e a levantamentos anteriormente realizados para determinar a ocorrência de biovares de R. solanacearum em lavouras de batata, ficou estabelecida a predominância, quase que absoluta, de estirpes da biovar 2 do patogeno. Este trabalho demonstrou que as condições de clima e solo do RS possibilitam a ocorrência das duas biovares, c que a predominância de uma delas é determinada pela espécie hospedeira. Departamento de Diagnóstico e Pesquisa Agropecuária. Secretaria de Agricultura, Pecuária e Desenvolvimento Rural2007-12-20info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://revistapag.agricultura.rs.gov.br/ojs/index.php/revistapag/article/view/268Pesquisa Agropecuária Gaúcha; Vol 12 No 1/2 (2006): PESQ. AGROP. GAÚCHA; 31-36Pesquisa Agropecuária Gaúcha; v. 12 n. 1/2 (2006): PESQ. AGROP. GAÚCHA; 31-362595-76860104-9070reponame:Pesquisa Agropecuária Gaúcha (Online)instname:Secretaria de Agricultura, Pecuária e Desenvolvimento Rural do Rio Grande do Sulinstacron:SEAPDRporhttp://revistapag.agricultura.rs.gov.br/ojs/index.php/revistapag/article/view/268/232Silveira, José Ricardo Pfeifer Ruprecht, Vivian Caroline Silva, Mônica de Medeiros Lisboa, Bruno Brito Vargas, Luciano Kayser Oliveira, Andréia Mara Rottainfo:eu-repo/semantics/openAccess2024-04-22T12:35:09Zoai:ojs.revistapag.agricultura.rs.gov.br:article/268Revistahttp://revistapag.agricultura.rs.gov.br/ojs/index.php/revistapagPUBhttp://revistapag.agricultura.rs.gov.br/ojs/index.php/revistapag/oaipag@agricultura.rs.gov.br||2595-76860104-9070opendoar:2024-04-22T12:35:09Pesquisa Agropecuária Gaúcha (Online) - Secretaria de Agricultura, Pecuária e Desenvolvimento Rural do Rio Grande do Sulfalse |
dc.title.none.fl_str_mv |
Predominante of Ralstonia solanacearum biovar 1 in vegetable crops under crop in Rio Grande do Sul State Predominância da biovar 1 de Ralstonia solanacearum em olerícolas cultivadas no Estado do Rio Grande do Sul |
title |
Predominante of Ralstonia solanacearum biovar 1 in vegetable crops under crop in Rio Grande do Sul State |
spellingShingle |
Predominante of Ralstonia solanacearum biovar 1 in vegetable crops under crop in Rio Grande do Sul State Silveira, José Ricardo Pfeifer epidemiology, bacterial wilt, biovar, tomato, eggplant, tobacco cpidemiologia, murcha bacteriana, biovar, tomate, berinjela, fumo |
title_short |
Predominante of Ralstonia solanacearum biovar 1 in vegetable crops under crop in Rio Grande do Sul State |
title_full |
Predominante of Ralstonia solanacearum biovar 1 in vegetable crops under crop in Rio Grande do Sul State |
title_fullStr |
Predominante of Ralstonia solanacearum biovar 1 in vegetable crops under crop in Rio Grande do Sul State |
title_full_unstemmed |
Predominante of Ralstonia solanacearum biovar 1 in vegetable crops under crop in Rio Grande do Sul State |
title_sort |
Predominante of Ralstonia solanacearum biovar 1 in vegetable crops under crop in Rio Grande do Sul State |
author |
Silveira, José Ricardo Pfeifer |
author_facet |
Silveira, José Ricardo Pfeifer Ruprecht, Vivian Caroline Silva, Mônica de Medeiros Lisboa, Bruno Brito Vargas, Luciano Kayser Oliveira, Andréia Mara Rotta |
author_role |
author |
author2 |
Ruprecht, Vivian Caroline Silva, Mônica de Medeiros Lisboa, Bruno Brito Vargas, Luciano Kayser Oliveira, Andréia Mara Rotta |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Silveira, José Ricardo Pfeifer Ruprecht, Vivian Caroline Silva, Mônica de Medeiros Lisboa, Bruno Brito Vargas, Luciano Kayser Oliveira, Andréia Mara Rotta |
dc.subject.por.fl_str_mv |
epidemiology, bacterial wilt, biovar, tomato, eggplant, tobacco cpidemiologia, murcha bacteriana, biovar, tomate, berinjela, fumo |
topic |
epidemiology, bacterial wilt, biovar, tomato, eggplant, tobacco cpidemiologia, murcha bacteriana, biovar, tomate, berinjela, fumo |
description |
In ordcr to verify the occurrence and distribution of biovars of Ralstonia solanacearum in vegetable crops under crop in the Statc of Rio Grande do Sul, Brazil. samples of tomato, eggplant and tobacco showing symptoms of bacterial wilt were collectcd in the spring of 2004, in 12 fanes from four citics: Maquiné, Caxias do Sul, Sobradinho and Santa Cruz do Sul. The identification of the bacterial species was carried out by the immunological test of ELISA and thc classification in biovar was based on the oxidative metabolism of alcohols and sugars and in the PCR fingerprinting. All thc 54 isolatcs were identificd as biovar 1. l-lowever, the amplification of DNA with the primas T3A and T5A resulted in a profile different from that expectcd for R. solanacearum strains, showing the amplification of an additional product with 650 bp. Dueto its dimane conditions and according to previous surveys in potato flelds, it was cstablished thc predominante of the biovar 2 of R. solanacearum. This study demonstrated that conditions of climate and soil in Rio Grande do Sul State pennit the occurrence of both biovars I and 2 and that the predominante of one or other is determined by the host spccics. |
publishDate |
2007 |
dc.date.none.fl_str_mv |
2007-12-20 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://revistapag.agricultura.rs.gov.br/ojs/index.php/revistapag/article/view/268 |
url |
http://revistapag.agricultura.rs.gov.br/ojs/index.php/revistapag/article/view/268 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
http://revistapag.agricultura.rs.gov.br/ojs/index.php/revistapag/article/view/268/232 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Departamento de Diagnóstico e Pesquisa Agropecuária. Secretaria de Agricultura, Pecuária e Desenvolvimento Rural |
publisher.none.fl_str_mv |
Departamento de Diagnóstico e Pesquisa Agropecuária. Secretaria de Agricultura, Pecuária e Desenvolvimento Rural |
dc.source.none.fl_str_mv |
Pesquisa Agropecuária Gaúcha; Vol 12 No 1/2 (2006): PESQ. AGROP. GAÚCHA; 31-36 Pesquisa Agropecuária Gaúcha; v. 12 n. 1/2 (2006): PESQ. AGROP. GAÚCHA; 31-36 2595-7686 0104-9070 reponame:Pesquisa Agropecuária Gaúcha (Online) instname:Secretaria de Agricultura, Pecuária e Desenvolvimento Rural do Rio Grande do Sul instacron:SEAPDR |
instname_str |
Secretaria de Agricultura, Pecuária e Desenvolvimento Rural do Rio Grande do Sul |
instacron_str |
SEAPDR |
institution |
SEAPDR |
reponame_str |
Pesquisa Agropecuária Gaúcha (Online) |
collection |
Pesquisa Agropecuária Gaúcha (Online) |
repository.name.fl_str_mv |
Pesquisa Agropecuária Gaúcha (Online) - Secretaria de Agricultura, Pecuária e Desenvolvimento Rural do Rio Grande do Sul |
repository.mail.fl_str_mv |
pag@agricultura.rs.gov.br|| |
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1797068772220076032 |