Predominante of Ralstonia solanacearum biovar 1 in vegetable crops under crop in Rio Grande do Sul State

Detalhes bibliográficos
Autor(a) principal: Silveira, José Ricardo Pfeifer
Data de Publicação: 2007
Outros Autores: Ruprecht, Vivian Caroline, Silva, Mônica de Medeiros, Lisboa, Bruno Brito, Vargas, Luciano Kayser, Oliveira, Andréia Mara Rotta
Tipo de documento: Artigo
Idioma: por
Título da fonte: Pesquisa Agropecuária Gaúcha (Online)
Texto Completo: http://revistapag.agricultura.rs.gov.br/ojs/index.php/revistapag/article/view/268
Resumo: In ordcr to verify the occurrence and distribution of biovars of Ralstonia solanacearum in vegetable crops under crop in the Statc of Rio Grande do Sul, Brazil. samples of tomato, eggplant and tobacco showing symptoms of bacterial wilt were collectcd in the spring of 2004, in 12 fanes from four citics: Maquiné, Caxias do Sul, Sobradinho and Santa Cruz do Sul. The identification of the bacterial species was carried out by the immunological test of ELISA and thc classification in biovar was based on the oxidative metabolism of alcohols and sugars and in the PCR fingerprinting. All thc 54 isolatcs were identificd as biovar 1. l-lowever, the amplification of DNA with the primas T3A and T5A resulted in a profile different from that expectcd for R. solanacearum strains, showing the amplification of an additional product with 650 bp. Dueto its dimane conditions and according to previous surveys in potato flelds, it was cstablished thc predominante of the biovar 2 of R. solanacearum. This study demonstrated that conditions of climate and soil in Rio Grande do Sul State pennit the occurrence of both biovars I and 2 and that the predominante of one or other is determined by the host spccics.
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spelling Predominante of Ralstonia solanacearum biovar 1 in vegetable crops under crop in Rio Grande do Sul State Predominância da biovar 1 de Ralstonia solanacearum em olerícolas cultivadas no Estado do Rio Grande do Sulepidemiology, bacterial wilt, biovar, tomato, eggplant, tobaccocpidemiologia, murcha bacteriana, biovar, tomate, berinjela, fumoIn ordcr to verify the occurrence and distribution of biovars of Ralstonia solanacearum in vegetable crops under crop in the Statc of Rio Grande do Sul, Brazil. samples of tomato, eggplant and tobacco showing symptoms of bacterial wilt were collectcd in the spring of 2004, in 12 fanes from four citics: Maquiné, Caxias do Sul, Sobradinho and Santa Cruz do Sul. The identification of the bacterial species was carried out by the immunological test of ELISA and thc classification in biovar was based on the oxidative metabolism of alcohols and sugars and in the PCR fingerprinting. All thc 54 isolatcs were identificd as biovar 1. l-lowever, the amplification of DNA with the primas T3A and T5A resulted in a profile different from that expectcd for R. solanacearum strains, showing the amplification of an additional product with 650 bp. Dueto its dimane conditions and according to previous surveys in potato flelds, it was cstablished thc predominante of the biovar 2 of R. solanacearum. This study demonstrated that conditions of climate and soil in Rio Grande do Sul State pennit the occurrence of both biovars I and 2 and that the predominante of one or other is determined by the host spccics. Com a finalidade de verificar a ocorrência e a distribuição de biovares de Ralstonia solanacearum em olericolas cultivadas no Estado do Rio Grande do Sul, plantas de tomate, berinjela e fumo com sintonias de murcha bacteriana foram coletadas, na primavera de 2004, em 12 lavouras de quatro municípios: Maquiné, Caxias do Sul, Sobradinho e Santa Cruz do Sul. A determinação da espécie foi feita com teste sorológico através de ELISA c a da biovar, com base no metabolismo oxidativo de açúcares e álcoois e por PCR. Todos os 54 isolados foram identificados como biovar I. Porém, a amplificação por PCR com os oligonucleolídeos iniciadores T3A e T5A resultaram em tipo diferente de perfil daqueles previstos para estirpes deste patógcno, com a amplificação de um produto de adicional de 650 pb. Devido às condições climáticas do Estado do Rio Grande do Sul e a levantamentos anteriormente realizados para determinar a ocorrência de biovares de R. solanacearum em lavouras de batata, ficou estabelecida a predominância, quase que absoluta, de estirpes da biovar 2 do patogeno. Este trabalho demonstrou que as condições de clima e solo do RS possibilitam a ocorrência das duas biovares, c que a predominância de uma delas é determinada pela espécie hospedeira. Departamento de Diagnóstico e Pesquisa Agropecuária. Secretaria de Agricultura, Pecuária e Desenvolvimento Rural2007-12-20info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://revistapag.agricultura.rs.gov.br/ojs/index.php/revistapag/article/view/268Pesquisa Agropecuária Gaúcha; Vol 12 No 1/2 (2006): PESQ. AGROP. GAÚCHA; 31-36Pesquisa Agropecuária Gaúcha; v. 12 n. 1/2 (2006): PESQ. AGROP. GAÚCHA; 31-362595-76860104-9070reponame:Pesquisa Agropecuária Gaúcha (Online)instname:Secretaria de Agricultura, Pecuária e Desenvolvimento Rural do Rio Grande do Sulinstacron:SEAPDRporhttp://revistapag.agricultura.rs.gov.br/ojs/index.php/revistapag/article/view/268/232Silveira, José Ricardo Pfeifer Ruprecht, Vivian Caroline Silva, Mônica de Medeiros Lisboa, Bruno Brito Vargas, Luciano Kayser Oliveira, Andréia Mara Rottainfo:eu-repo/semantics/openAccess2024-04-22T12:35:09Zoai:ojs.revistapag.agricultura.rs.gov.br:article/268Revistahttp://revistapag.agricultura.rs.gov.br/ojs/index.php/revistapagPUBhttp://revistapag.agricultura.rs.gov.br/ojs/index.php/revistapag/oaipag@agricultura.rs.gov.br||2595-76860104-9070opendoar:2024-04-22T12:35:09Pesquisa Agropecuária Gaúcha (Online) - Secretaria de Agricultura, Pecuária e Desenvolvimento Rural do Rio Grande do Sulfalse
dc.title.none.fl_str_mv Predominante of Ralstonia solanacearum biovar 1 in vegetable crops under crop in Rio Grande do Sul State
Predominância da biovar 1 de Ralstonia solanacearum em olerícolas cultivadas no Estado do Rio Grande do Sul
title Predominante of Ralstonia solanacearum biovar 1 in vegetable crops under crop in Rio Grande do Sul State
spellingShingle Predominante of Ralstonia solanacearum biovar 1 in vegetable crops under crop in Rio Grande do Sul State
Silveira, José Ricardo Pfeifer
epidemiology, bacterial wilt, biovar, tomato, eggplant, tobacco
cpidemiologia, murcha bacteriana, biovar, tomate, berinjela, fumo
title_short Predominante of Ralstonia solanacearum biovar 1 in vegetable crops under crop in Rio Grande do Sul State
title_full Predominante of Ralstonia solanacearum biovar 1 in vegetable crops under crop in Rio Grande do Sul State
title_fullStr Predominante of Ralstonia solanacearum biovar 1 in vegetable crops under crop in Rio Grande do Sul State
title_full_unstemmed Predominante of Ralstonia solanacearum biovar 1 in vegetable crops under crop in Rio Grande do Sul State
title_sort Predominante of Ralstonia solanacearum biovar 1 in vegetable crops under crop in Rio Grande do Sul State
author Silveira, José Ricardo Pfeifer
author_facet Silveira, José Ricardo Pfeifer
Ruprecht, Vivian Caroline
Silva, Mônica de Medeiros
Lisboa, Bruno Brito
Vargas, Luciano Kayser
Oliveira, Andréia Mara Rotta
author_role author
author2 Ruprecht, Vivian Caroline
Silva, Mônica de Medeiros
Lisboa, Bruno Brito
Vargas, Luciano Kayser
Oliveira, Andréia Mara Rotta
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Silveira, José Ricardo Pfeifer
Ruprecht, Vivian Caroline
Silva, Mônica de Medeiros
Lisboa, Bruno Brito
Vargas, Luciano Kayser
Oliveira, Andréia Mara Rotta
dc.subject.por.fl_str_mv epidemiology, bacterial wilt, biovar, tomato, eggplant, tobacco
cpidemiologia, murcha bacteriana, biovar, tomate, berinjela, fumo
topic epidemiology, bacterial wilt, biovar, tomato, eggplant, tobacco
cpidemiologia, murcha bacteriana, biovar, tomate, berinjela, fumo
description In ordcr to verify the occurrence and distribution of biovars of Ralstonia solanacearum in vegetable crops under crop in the Statc of Rio Grande do Sul, Brazil. samples of tomato, eggplant and tobacco showing symptoms of bacterial wilt were collectcd in the spring of 2004, in 12 fanes from four citics: Maquiné, Caxias do Sul, Sobradinho and Santa Cruz do Sul. The identification of the bacterial species was carried out by the immunological test of ELISA and thc classification in biovar was based on the oxidative metabolism of alcohols and sugars and in the PCR fingerprinting. All thc 54 isolatcs were identificd as biovar 1. l-lowever, the amplification of DNA with the primas T3A and T5A resulted in a profile different from that expectcd for R. solanacearum strains, showing the amplification of an additional product with 650 bp. Dueto its dimane conditions and according to previous surveys in potato flelds, it was cstablished thc predominante of the biovar 2 of R. solanacearum. This study demonstrated that conditions of climate and soil in Rio Grande do Sul State pennit the occurrence of both biovars I and 2 and that the predominante of one or other is determined by the host spccics.
publishDate 2007
dc.date.none.fl_str_mv 2007-12-20
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://revistapag.agricultura.rs.gov.br/ojs/index.php/revistapag/article/view/268
url http://revistapag.agricultura.rs.gov.br/ojs/index.php/revistapag/article/view/268
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv http://revistapag.agricultura.rs.gov.br/ojs/index.php/revistapag/article/view/268/232
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Departamento de Diagnóstico e Pesquisa Agropecuária. Secretaria de Agricultura, Pecuária e Desenvolvimento Rural
publisher.none.fl_str_mv Departamento de Diagnóstico e Pesquisa Agropecuária. Secretaria de Agricultura, Pecuária e Desenvolvimento Rural
dc.source.none.fl_str_mv Pesquisa Agropecuária Gaúcha; Vol 12 No 1/2 (2006): PESQ. AGROP. GAÚCHA; 31-36
Pesquisa Agropecuária Gaúcha; v. 12 n. 1/2 (2006): PESQ. AGROP. GAÚCHA; 31-36
2595-7686
0104-9070
reponame:Pesquisa Agropecuária Gaúcha (Online)
instname:Secretaria de Agricultura, Pecuária e Desenvolvimento Rural do Rio Grande do Sul
instacron:SEAPDR
instname_str Secretaria de Agricultura, Pecuária e Desenvolvimento Rural do Rio Grande do Sul
instacron_str SEAPDR
institution SEAPDR
reponame_str Pesquisa Agropecuária Gaúcha (Online)
collection Pesquisa Agropecuária Gaúcha (Online)
repository.name.fl_str_mv Pesquisa Agropecuária Gaúcha (Online) - Secretaria de Agricultura, Pecuária e Desenvolvimento Rural do Rio Grande do Sul
repository.mail.fl_str_mv pag@agricultura.rs.gov.br||
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