Detection of mitochondrial DNA restriction site differences between the subspecies of Melipona quadrifasciata Lepeletier (Hymenoptera: Apidae: Meliponini)

Detalhes bibliográficos
Autor(a) principal: Moretto,Geraldo
Data de Publicação: 2005
Outros Autores: Arias,Maria C.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Neotropical entomology (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2005000300004
Resumo: The endemic range of the stingless bee Melipona quadrifasciata Lepeletier extends from the southern most state in Brazil, Rio Grande do Sul, up to the state of Paraíba, where it originally inhabited the Atlantic Rain Forest. Two subspecies have been morphologically recognized based on the tergal band pattern (yellow stripes across the abdomen), M. q. quadrifasciata and M. q. anthidioides. The objective of the present study was to characterize restriction site variation in the mitochondrial DNA of each subspecies. Four colonies of each subspecies were sampled. One individual per colony was used for DNA extraction and further analysis through amplification of nine fragments of the mitochondrial genome and digestion with 13 restriction enzymes (PCR+RFLP). Three enzymes showed restriction pattern differences between the subspecies. Those differences will be useful to determine the maternal origin of hybrids colonies, which may occur naturally or due to colony transportation among breeders.
id SEB-2_1fbb7d96778eb892b49896a35ab16158
oai_identifier_str oai:scielo:S1519-566X2005000300004
network_acronym_str SEB-2
network_name_str Neotropical entomology (Online)
repository_id_str
spelling Detection of mitochondrial DNA restriction site differences between the subspecies of Melipona quadrifasciata Lepeletier (Hymenoptera: Apidae: Meliponini)Restriction enzymepolymorphismPCR+RLFPmtDNAThe endemic range of the stingless bee Melipona quadrifasciata Lepeletier extends from the southern most state in Brazil, Rio Grande do Sul, up to the state of Paraíba, where it originally inhabited the Atlantic Rain Forest. Two subspecies have been morphologically recognized based on the tergal band pattern (yellow stripes across the abdomen), M. q. quadrifasciata and M. q. anthidioides. The objective of the present study was to characterize restriction site variation in the mitochondrial DNA of each subspecies. Four colonies of each subspecies were sampled. One individual per colony was used for DNA extraction and further analysis through amplification of nine fragments of the mitochondrial genome and digestion with 13 restriction enzymes (PCR+RFLP). Three enzymes showed restriction pattern differences between the subspecies. Those differences will be useful to determine the maternal origin of hybrids colonies, which may occur naturally or due to colony transportation among breeders.Sociedade Entomológica do Brasil2005-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2005000300004Neotropical Entomology v.34 n.3 2005reponame:Neotropical entomology (Online)instname:Sociedade Entomológica do Brasil (SEB)instacron:SEB10.1590/S1519-566X2005000300004info:eu-repo/semantics/openAccessMoretto,GeraldoArias,Maria C.eng2005-09-12T00:00:00Zoai:scielo:S1519-566X2005000300004Revistahttp://www.scielo.br/neONGhttps://old.scielo.br/oai/scielo-oai.php||editor@seb.org.br1678-80521519-566Xopendoar:2005-09-12T00:00Neotropical entomology (Online) - Sociedade Entomológica do Brasil (SEB)false
dc.title.none.fl_str_mv Detection of mitochondrial DNA restriction site differences between the subspecies of Melipona quadrifasciata Lepeletier (Hymenoptera: Apidae: Meliponini)
title Detection of mitochondrial DNA restriction site differences between the subspecies of Melipona quadrifasciata Lepeletier (Hymenoptera: Apidae: Meliponini)
spellingShingle Detection of mitochondrial DNA restriction site differences between the subspecies of Melipona quadrifasciata Lepeletier (Hymenoptera: Apidae: Meliponini)
Moretto,Geraldo
Restriction enzyme
polymorphism
PCR+RLFP
mtDNA
title_short Detection of mitochondrial DNA restriction site differences between the subspecies of Melipona quadrifasciata Lepeletier (Hymenoptera: Apidae: Meliponini)
title_full Detection of mitochondrial DNA restriction site differences between the subspecies of Melipona quadrifasciata Lepeletier (Hymenoptera: Apidae: Meliponini)
title_fullStr Detection of mitochondrial DNA restriction site differences between the subspecies of Melipona quadrifasciata Lepeletier (Hymenoptera: Apidae: Meliponini)
title_full_unstemmed Detection of mitochondrial DNA restriction site differences between the subspecies of Melipona quadrifasciata Lepeletier (Hymenoptera: Apidae: Meliponini)
title_sort Detection of mitochondrial DNA restriction site differences between the subspecies of Melipona quadrifasciata Lepeletier (Hymenoptera: Apidae: Meliponini)
author Moretto,Geraldo
author_facet Moretto,Geraldo
Arias,Maria C.
author_role author
author2 Arias,Maria C.
author2_role author
dc.contributor.author.fl_str_mv Moretto,Geraldo
Arias,Maria C.
dc.subject.por.fl_str_mv Restriction enzyme
polymorphism
PCR+RLFP
mtDNA
topic Restriction enzyme
polymorphism
PCR+RLFP
mtDNA
description The endemic range of the stingless bee Melipona quadrifasciata Lepeletier extends from the southern most state in Brazil, Rio Grande do Sul, up to the state of Paraíba, where it originally inhabited the Atlantic Rain Forest. Two subspecies have been morphologically recognized based on the tergal band pattern (yellow stripes across the abdomen), M. q. quadrifasciata and M. q. anthidioides. The objective of the present study was to characterize restriction site variation in the mitochondrial DNA of each subspecies. Four colonies of each subspecies were sampled. One individual per colony was used for DNA extraction and further analysis through amplification of nine fragments of the mitochondrial genome and digestion with 13 restriction enzymes (PCR+RFLP). Three enzymes showed restriction pattern differences between the subspecies. Those differences will be useful to determine the maternal origin of hybrids colonies, which may occur naturally or due to colony transportation among breeders.
publishDate 2005
dc.date.none.fl_str_mv 2005-06-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2005000300004
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2005000300004
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1519-566X2005000300004
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Entomológica do Brasil
publisher.none.fl_str_mv Sociedade Entomológica do Brasil
dc.source.none.fl_str_mv Neotropical Entomology v.34 n.3 2005
reponame:Neotropical entomology (Online)
instname:Sociedade Entomológica do Brasil (SEB)
instacron:SEB
instname_str Sociedade Entomológica do Brasil (SEB)
instacron_str SEB
institution SEB
reponame_str Neotropical entomology (Online)
collection Neotropical entomology (Online)
repository.name.fl_str_mv Neotropical entomology (Online) - Sociedade Entomológica do Brasil (SEB)
repository.mail.fl_str_mv ||editor@seb.org.br
_version_ 1754820845410189312

Registros relacionados