Isolation and maintenance of symbiotic fungi of ants in the tribe Attini (Hymenoptera: Formicidae)

Detalhes bibliográficos
Autor(a) principal: Silva-Pinhati,Ana C.O.
Data de Publicação: 2005
Outros Autores: Bacci Jr.,Maurício, Siqueira,Célia G., Silva,Aline, Pagnocca,Fernando C., Bueno,Odair C., Hebling,Maria J.A.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Neotropical entomology (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2005000100001
Resumo: The isolation and maintenance of symbiotic basidiomycete fungi living in association with ants of the tribe Attini has been hindered by the slow growth rate of these fungi and the presence of other microorganisms on the surface of the material which the ants maintain inside their nests to provide a growth substrate for their symbiont. In this paper we describe a method which increases the efficiency of isolation of these fungal symbionts by over seven fold as compared to traditional isolation procedures. Underground nests of attine ants of the genera Atta, Acromyrmex, Trachymyrmex and Mycetarotes were located, from which samples containing the fungal symbiont and ants were collected and transported to the laboratory where the ants were able to clean the fungal culture and stimulate its growth. As the symbiotic fungus grew, portions of its mycelium were collected and transferred to solid Yeast Nitrogen Base culture medium containing glucose and chloramphenicol. To facilitate the maintenance of the isolates in laboratory cultures, several nutrients were tested to formulate a complex culture medium for fast fungal growth and long-term storage. We successfully applied this methodology to the fungal symbionts of all the ant genera studied, thus producing a useful tool for the creation and maintenance of a comprehensive collection of fungi symbiotic of ants in the tribe Attini.
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spelling Isolation and maintenance of symbiotic fungi of ants in the tribe Attini (Hymenoptera: Formicidae)AttaAcromyrmexTrachymyrmexMycetarotesbasidiomycetesymbiosisThe isolation and maintenance of symbiotic basidiomycete fungi living in association with ants of the tribe Attini has been hindered by the slow growth rate of these fungi and the presence of other microorganisms on the surface of the material which the ants maintain inside their nests to provide a growth substrate for their symbiont. In this paper we describe a method which increases the efficiency of isolation of these fungal symbionts by over seven fold as compared to traditional isolation procedures. Underground nests of attine ants of the genera Atta, Acromyrmex, Trachymyrmex and Mycetarotes were located, from which samples containing the fungal symbiont and ants were collected and transported to the laboratory where the ants were able to clean the fungal culture and stimulate its growth. As the symbiotic fungus grew, portions of its mycelium were collected and transferred to solid Yeast Nitrogen Base culture medium containing glucose and chloramphenicol. To facilitate the maintenance of the isolates in laboratory cultures, several nutrients were tested to formulate a complex culture medium for fast fungal growth and long-term storage. We successfully applied this methodology to the fungal symbionts of all the ant genera studied, thus producing a useful tool for the creation and maintenance of a comprehensive collection of fungi symbiotic of ants in the tribe Attini.Sociedade Entomológica do Brasil2005-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2005000100001Neotropical Entomology v.34 n.1 2005reponame:Neotropical entomology (Online)instname:Sociedade Entomológica do Brasil (SEB)instacron:SEB10.1590/S1519-566X2005000100001info:eu-repo/semantics/openAccessSilva-Pinhati,Ana C.O.Bacci Jr.,MaurícioSiqueira,Célia G.Silva,AlinePagnocca,Fernando C.Bueno,Odair C.Hebling,Maria J.A.eng2005-03-22T00:00:00Zoai:scielo:S1519-566X2005000100001Revistahttp://www.scielo.br/neONGhttps://old.scielo.br/oai/scielo-oai.php||editor@seb.org.br1678-80521519-566Xopendoar:2005-03-22T00:00Neotropical entomology (Online) - Sociedade Entomológica do Brasil (SEB)false
dc.title.none.fl_str_mv Isolation and maintenance of symbiotic fungi of ants in the tribe Attini (Hymenoptera: Formicidae)
title Isolation and maintenance of symbiotic fungi of ants in the tribe Attini (Hymenoptera: Formicidae)
spellingShingle Isolation and maintenance of symbiotic fungi of ants in the tribe Attini (Hymenoptera: Formicidae)
Silva-Pinhati,Ana C.O.
Atta
Acromyrmex
Trachymyrmex
Mycetarotes
basidiomycete
symbiosis
title_short Isolation and maintenance of symbiotic fungi of ants in the tribe Attini (Hymenoptera: Formicidae)
title_full Isolation and maintenance of symbiotic fungi of ants in the tribe Attini (Hymenoptera: Formicidae)
title_fullStr Isolation and maintenance of symbiotic fungi of ants in the tribe Attini (Hymenoptera: Formicidae)
title_full_unstemmed Isolation and maintenance of symbiotic fungi of ants in the tribe Attini (Hymenoptera: Formicidae)
title_sort Isolation and maintenance of symbiotic fungi of ants in the tribe Attini (Hymenoptera: Formicidae)
author Silva-Pinhati,Ana C.O.
author_facet Silva-Pinhati,Ana C.O.
Bacci Jr.,Maurício
Siqueira,Célia G.
Silva,Aline
Pagnocca,Fernando C.
Bueno,Odair C.
Hebling,Maria J.A.
author_role author
author2 Bacci Jr.,Maurício
Siqueira,Célia G.
Silva,Aline
Pagnocca,Fernando C.
Bueno,Odair C.
Hebling,Maria J.A.
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Silva-Pinhati,Ana C.O.
Bacci Jr.,Maurício
Siqueira,Célia G.
Silva,Aline
Pagnocca,Fernando C.
Bueno,Odair C.
Hebling,Maria J.A.
dc.subject.por.fl_str_mv Atta
Acromyrmex
Trachymyrmex
Mycetarotes
basidiomycete
symbiosis
topic Atta
Acromyrmex
Trachymyrmex
Mycetarotes
basidiomycete
symbiosis
description The isolation and maintenance of symbiotic basidiomycete fungi living in association with ants of the tribe Attini has been hindered by the slow growth rate of these fungi and the presence of other microorganisms on the surface of the material which the ants maintain inside their nests to provide a growth substrate for their symbiont. In this paper we describe a method which increases the efficiency of isolation of these fungal symbionts by over seven fold as compared to traditional isolation procedures. Underground nests of attine ants of the genera Atta, Acromyrmex, Trachymyrmex and Mycetarotes were located, from which samples containing the fungal symbiont and ants were collected and transported to the laboratory where the ants were able to clean the fungal culture and stimulate its growth. As the symbiotic fungus grew, portions of its mycelium were collected and transferred to solid Yeast Nitrogen Base culture medium containing glucose and chloramphenicol. To facilitate the maintenance of the isolates in laboratory cultures, several nutrients were tested to formulate a complex culture medium for fast fungal growth and long-term storage. We successfully applied this methodology to the fungal symbionts of all the ant genera studied, thus producing a useful tool for the creation and maintenance of a comprehensive collection of fungi symbiotic of ants in the tribe Attini.
publishDate 2005
dc.date.none.fl_str_mv 2005-02-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2005000100001
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2005000100001
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1519-566X2005000100001
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Entomológica do Brasil
publisher.none.fl_str_mv Sociedade Entomológica do Brasil
dc.source.none.fl_str_mv Neotropical Entomology v.34 n.1 2005
reponame:Neotropical entomology (Online)
instname:Sociedade Entomológica do Brasil (SEB)
instacron:SEB
instname_str Sociedade Entomológica do Brasil (SEB)
instacron_str SEB
institution SEB
reponame_str Neotropical entomology (Online)
collection Neotropical entomology (Online)
repository.name.fl_str_mv Neotropical entomology (Online) - Sociedade Entomológica do Brasil (SEB)
repository.mail.fl_str_mv ||editor@seb.org.br
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