CLONAL PROPAGATION OF NEEM (Azadirachta indica A. Juss.) VIA DIRECT AND INDIRECT IN VITRO REGENERATION

Detalhes bibliográficos
Autor(a) principal: Houllou,Laureen Michelle
Data de Publicação: 2015
Outros Autores: Souza,Robson Antônio de, Santos,Elizabete Cristina Pacheco dos, Silva,José Jackson Pereira da, Barbosa,Marta Ribeiro, Sauvé,Jean Philippe Guimarães, Harand,Wolfgang
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista Árvore (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-67622015000300439
Resumo: ABSTRACTThe study was conducted with shoot tip explants of neem (Azadirachta indica A. Juss) to identify a viable regenerative process. Shoot tips were obtained from neem embryos cultured alternatingly in DKW medium supplemented with BAP and medium without hormones. Initial shoot development was influenced by cotyledon presence. Basal callus, excised from in vitro stem base, also presented organogenic potential. In some cases, plant lines, obtained from each seed, presented different characteristics. The most common characteristic observed in vitro was callus formation at the stem base. However, the rarest characteristics were stem callus formation and leaf senescence. The regenerated shoot tips were further subculture and rooted on a medium supplemented with IBA so that complete plants could be obtained. The rooted plants were transplanted to a greenhouse and successfully acclimatized. No significant differences in in vivo development were observed between neem plants from callus and from shoot tip propagation.
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spelling CLONAL PROPAGATION OF NEEM (Azadirachta indica A. Juss.) VIA DIRECT AND INDIRECT IN VITRO REGENERATIONMicropropagationIn vitro developmental characteristicsCallus organogenesisABSTRACTThe study was conducted with shoot tip explants of neem (Azadirachta indica A. Juss) to identify a viable regenerative process. Shoot tips were obtained from neem embryos cultured alternatingly in DKW medium supplemented with BAP and medium without hormones. Initial shoot development was influenced by cotyledon presence. Basal callus, excised from in vitro stem base, also presented organogenic potential. In some cases, plant lines, obtained from each seed, presented different characteristics. The most common characteristic observed in vitro was callus formation at the stem base. However, the rarest characteristics were stem callus formation and leaf senescence. The regenerated shoot tips were further subculture and rooted on a medium supplemented with IBA so that complete plants could be obtained. The rooted plants were transplanted to a greenhouse and successfully acclimatized. No significant differences in in vivo development were observed between neem plants from callus and from shoot tip propagation.Sociedade de Investigações Florestais2015-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-67622015000300439Revista Árvore v.39 n.3 2015reponame:Revista Árvore (Online)instname:Universidade Federal de Viçosa (UFV)instacron:SIF10.1590/0100-67622015000300004info:eu-repo/semantics/openAccessHoullou,Laureen MichelleSouza,Robson Antônio deSantos,Elizabete Cristina Pacheco dosSilva,José Jackson Pereira daBarbosa,Marta RibeiroSauvé,Jean Philippe GuimarãesHarand,Wolfgangeng2015-08-26T00:00:00Zoai:scielo:S0100-67622015000300439Revistahttp://www.scielo.br/revistas/rarv/iaboutj.htmPUBhttps://old.scielo.br/oai/scielo-oai.php||r.arvore@ufv.br1806-90880100-6762opendoar:2015-08-26T00:00Revista Árvore (Online) - Universidade Federal de Viçosa (UFV)false
dc.title.none.fl_str_mv CLONAL PROPAGATION OF NEEM (Azadirachta indica A. Juss.) VIA DIRECT AND INDIRECT IN VITRO REGENERATION
title CLONAL PROPAGATION OF NEEM (Azadirachta indica A. Juss.) VIA DIRECT AND INDIRECT IN VITRO REGENERATION
spellingShingle CLONAL PROPAGATION OF NEEM (Azadirachta indica A. Juss.) VIA DIRECT AND INDIRECT IN VITRO REGENERATION
Houllou,Laureen Michelle
Micropropagation
In vitro developmental characteristics
Callus organogenesis
title_short CLONAL PROPAGATION OF NEEM (Azadirachta indica A. Juss.) VIA DIRECT AND INDIRECT IN VITRO REGENERATION
title_full CLONAL PROPAGATION OF NEEM (Azadirachta indica A. Juss.) VIA DIRECT AND INDIRECT IN VITRO REGENERATION
title_fullStr CLONAL PROPAGATION OF NEEM (Azadirachta indica A. Juss.) VIA DIRECT AND INDIRECT IN VITRO REGENERATION
title_full_unstemmed CLONAL PROPAGATION OF NEEM (Azadirachta indica A. Juss.) VIA DIRECT AND INDIRECT IN VITRO REGENERATION
title_sort CLONAL PROPAGATION OF NEEM (Azadirachta indica A. Juss.) VIA DIRECT AND INDIRECT IN VITRO REGENERATION
author Houllou,Laureen Michelle
author_facet Houllou,Laureen Michelle
Souza,Robson Antônio de
Santos,Elizabete Cristina Pacheco dos
Silva,José Jackson Pereira da
Barbosa,Marta Ribeiro
Sauvé,Jean Philippe Guimarães
Harand,Wolfgang
author_role author
author2 Souza,Robson Antônio de
Santos,Elizabete Cristina Pacheco dos
Silva,José Jackson Pereira da
Barbosa,Marta Ribeiro
Sauvé,Jean Philippe Guimarães
Harand,Wolfgang
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Houllou,Laureen Michelle
Souza,Robson Antônio de
Santos,Elizabete Cristina Pacheco dos
Silva,José Jackson Pereira da
Barbosa,Marta Ribeiro
Sauvé,Jean Philippe Guimarães
Harand,Wolfgang
dc.subject.por.fl_str_mv Micropropagation
In vitro developmental characteristics
Callus organogenesis
topic Micropropagation
In vitro developmental characteristics
Callus organogenesis
description ABSTRACTThe study was conducted with shoot tip explants of neem (Azadirachta indica A. Juss) to identify a viable regenerative process. Shoot tips were obtained from neem embryos cultured alternatingly in DKW medium supplemented with BAP and medium without hormones. Initial shoot development was influenced by cotyledon presence. Basal callus, excised from in vitro stem base, also presented organogenic potential. In some cases, plant lines, obtained from each seed, presented different characteristics. The most common characteristic observed in vitro was callus formation at the stem base. However, the rarest characteristics were stem callus formation and leaf senescence. The regenerated shoot tips were further subculture and rooted on a medium supplemented with IBA so that complete plants could be obtained. The rooted plants were transplanted to a greenhouse and successfully acclimatized. No significant differences in in vivo development were observed between neem plants from callus and from shoot tip propagation.
publishDate 2015
dc.date.none.fl_str_mv 2015-06-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-67622015000300439
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-67622015000300439
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/0100-67622015000300004
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade de Investigações Florestais
publisher.none.fl_str_mv Sociedade de Investigações Florestais
dc.source.none.fl_str_mv Revista Árvore v.39 n.3 2015
reponame:Revista Árvore (Online)
instname:Universidade Federal de Viçosa (UFV)
instacron:SIF
instname_str Universidade Federal de Viçosa (UFV)
instacron_str SIF
institution SIF
reponame_str Revista Árvore (Online)
collection Revista Árvore (Online)
repository.name.fl_str_mv Revista Árvore (Online) - Universidade Federal de Viçosa (UFV)
repository.mail.fl_str_mv ||r.arvore@ufv.br
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