PERMEABILIZATION, CELL WALL ULTRASTRUCTURE, AND GERMINATION OF BASIDIOSPORES OF THE ECTOMYCORRHIZAL FUNGUS Pisolithus microcarpus TREATED WITH DIFFERENT COMMERCIAL BRANDS OF BLEACH

Detalhes bibliográficos
Autor(a) principal: Silvério,Merielle Angélica Martines
Data de Publicação: 2021
Outros Autores: Nascimento,Jaqueline Maria do, Serrão,José Eduardo, Tótola,Marcos Rogério, Costa,Maurício Dutra
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista Árvore (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-67622021000100209
Resumo: ABSTRACT Basidiospores of the ectomycorrhizal fungus Pisolithus microcarpus have an impermeable cell wall, a characteristic that is possibly related to the low germination percentages of these propagules, which makes it difficult to obtain monokaryons and use these spores in inoculants. The objective of this study was to evaluate the effect of different concentrations of commercial bleach on the permeabilization of P. microcarpus basidiospores and to analyze the alterations caused in the cell wall ultrastructure and the viability and germination capacity of these propagules. Fungal basidiospores were collected in eucalyptus plantations and permeabilized using different bleach concentrations and exposure times. The basidiospores were then analyzed by scanning and transmission electron microscopy. The percentage of permeabilized basidiospores varied with the commercial brand, bleach concentration, and exposure time. Basidiospores of different basidiocarps differed in susceptibility to permeabilization treatment with bleach. Changes in the ultrastructure of permeabilized basidiospores were observed at bleach concentrations of 15 and 50 % for an exposure time of 40 s, with surface changes and loss of the spicules of the outermost layer of the wall. After permeabilization with 5 % bleach for 40 s, 80 % of the permeabilized spores were viable, resulting in the production of fungal colonies after 15 days of incubation of these propagules in the presence of Corymbia citriodora. However, the germination percentage obtained, 0.001 %, was similar to that of non-permeabilized basidiospores, indicating that other factors, besides cell wall permeability, are determinant for the germination process.
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spelling PERMEABILIZATION, CELL WALL ULTRASTRUCTURE, AND GERMINATION OF BASIDIOSPORES OF THE ECTOMYCORRHIZAL FUNGUS Pisolithus microcarpus TREATED WITH DIFFERENT COMMERCIAL BRANDS OF BLEACHEctomycorrhizaInoculantEucalyptusABSTRACT Basidiospores of the ectomycorrhizal fungus Pisolithus microcarpus have an impermeable cell wall, a characteristic that is possibly related to the low germination percentages of these propagules, which makes it difficult to obtain monokaryons and use these spores in inoculants. The objective of this study was to evaluate the effect of different concentrations of commercial bleach on the permeabilization of P. microcarpus basidiospores and to analyze the alterations caused in the cell wall ultrastructure and the viability and germination capacity of these propagules. Fungal basidiospores were collected in eucalyptus plantations and permeabilized using different bleach concentrations and exposure times. The basidiospores were then analyzed by scanning and transmission electron microscopy. The percentage of permeabilized basidiospores varied with the commercial brand, bleach concentration, and exposure time. Basidiospores of different basidiocarps differed in susceptibility to permeabilization treatment with bleach. Changes in the ultrastructure of permeabilized basidiospores were observed at bleach concentrations of 15 and 50 % for an exposure time of 40 s, with surface changes and loss of the spicules of the outermost layer of the wall. After permeabilization with 5 % bleach for 40 s, 80 % of the permeabilized spores were viable, resulting in the production of fungal colonies after 15 days of incubation of these propagules in the presence of Corymbia citriodora. However, the germination percentage obtained, 0.001 %, was similar to that of non-permeabilized basidiospores, indicating that other factors, besides cell wall permeability, are determinant for the germination process.Sociedade de Investigações Florestais2021-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-67622021000100209Revista Árvore v.45 2021reponame:Revista Árvore (Online)instname:Universidade Federal de Viçosa (UFV)instacron:SIF10.1590/1806-908820210000009info:eu-repo/semantics/openAccessSilvério,Merielle Angélica MartinesNascimento,Jaqueline Maria doSerrão,José EduardoTótola,Marcos RogérioCosta,Maurício Dutraeng2021-06-30T00:00:00Zoai:scielo:S0100-67622021000100209Revistahttp://www.scielo.br/revistas/rarv/iaboutj.htmPUBhttps://old.scielo.br/oai/scielo-oai.php||r.arvore@ufv.br1806-90880100-6762opendoar:2021-06-30T00:00Revista Árvore (Online) - Universidade Federal de Viçosa (UFV)false
dc.title.none.fl_str_mv PERMEABILIZATION, CELL WALL ULTRASTRUCTURE, AND GERMINATION OF BASIDIOSPORES OF THE ECTOMYCORRHIZAL FUNGUS Pisolithus microcarpus TREATED WITH DIFFERENT COMMERCIAL BRANDS OF BLEACH
title PERMEABILIZATION, CELL WALL ULTRASTRUCTURE, AND GERMINATION OF BASIDIOSPORES OF THE ECTOMYCORRHIZAL FUNGUS Pisolithus microcarpus TREATED WITH DIFFERENT COMMERCIAL BRANDS OF BLEACH
spellingShingle PERMEABILIZATION, CELL WALL ULTRASTRUCTURE, AND GERMINATION OF BASIDIOSPORES OF THE ECTOMYCORRHIZAL FUNGUS Pisolithus microcarpus TREATED WITH DIFFERENT COMMERCIAL BRANDS OF BLEACH
Silvério,Merielle Angélica Martines
Ectomycorrhiza
Inoculant
Eucalyptus
title_short PERMEABILIZATION, CELL WALL ULTRASTRUCTURE, AND GERMINATION OF BASIDIOSPORES OF THE ECTOMYCORRHIZAL FUNGUS Pisolithus microcarpus TREATED WITH DIFFERENT COMMERCIAL BRANDS OF BLEACH
title_full PERMEABILIZATION, CELL WALL ULTRASTRUCTURE, AND GERMINATION OF BASIDIOSPORES OF THE ECTOMYCORRHIZAL FUNGUS Pisolithus microcarpus TREATED WITH DIFFERENT COMMERCIAL BRANDS OF BLEACH
title_fullStr PERMEABILIZATION, CELL WALL ULTRASTRUCTURE, AND GERMINATION OF BASIDIOSPORES OF THE ECTOMYCORRHIZAL FUNGUS Pisolithus microcarpus TREATED WITH DIFFERENT COMMERCIAL BRANDS OF BLEACH
title_full_unstemmed PERMEABILIZATION, CELL WALL ULTRASTRUCTURE, AND GERMINATION OF BASIDIOSPORES OF THE ECTOMYCORRHIZAL FUNGUS Pisolithus microcarpus TREATED WITH DIFFERENT COMMERCIAL BRANDS OF BLEACH
title_sort PERMEABILIZATION, CELL WALL ULTRASTRUCTURE, AND GERMINATION OF BASIDIOSPORES OF THE ECTOMYCORRHIZAL FUNGUS Pisolithus microcarpus TREATED WITH DIFFERENT COMMERCIAL BRANDS OF BLEACH
author Silvério,Merielle Angélica Martines
author_facet Silvério,Merielle Angélica Martines
Nascimento,Jaqueline Maria do
Serrão,José Eduardo
Tótola,Marcos Rogério
Costa,Maurício Dutra
author_role author
author2 Nascimento,Jaqueline Maria do
Serrão,José Eduardo
Tótola,Marcos Rogério
Costa,Maurício Dutra
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Silvério,Merielle Angélica Martines
Nascimento,Jaqueline Maria do
Serrão,José Eduardo
Tótola,Marcos Rogério
Costa,Maurício Dutra
dc.subject.por.fl_str_mv Ectomycorrhiza
Inoculant
Eucalyptus
topic Ectomycorrhiza
Inoculant
Eucalyptus
description ABSTRACT Basidiospores of the ectomycorrhizal fungus Pisolithus microcarpus have an impermeable cell wall, a characteristic that is possibly related to the low germination percentages of these propagules, which makes it difficult to obtain monokaryons and use these spores in inoculants. The objective of this study was to evaluate the effect of different concentrations of commercial bleach on the permeabilization of P. microcarpus basidiospores and to analyze the alterations caused in the cell wall ultrastructure and the viability and germination capacity of these propagules. Fungal basidiospores were collected in eucalyptus plantations and permeabilized using different bleach concentrations and exposure times. The basidiospores were then analyzed by scanning and transmission electron microscopy. The percentage of permeabilized basidiospores varied with the commercial brand, bleach concentration, and exposure time. Basidiospores of different basidiocarps differed in susceptibility to permeabilization treatment with bleach. Changes in the ultrastructure of permeabilized basidiospores were observed at bleach concentrations of 15 and 50 % for an exposure time of 40 s, with surface changes and loss of the spicules of the outermost layer of the wall. After permeabilization with 5 % bleach for 40 s, 80 % of the permeabilized spores were viable, resulting in the production of fungal colonies after 15 days of incubation of these propagules in the presence of Corymbia citriodora. However, the germination percentage obtained, 0.001 %, was similar to that of non-permeabilized basidiospores, indicating that other factors, besides cell wall permeability, are determinant for the germination process.
publishDate 2021
dc.date.none.fl_str_mv 2021-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-67622021000100209
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-67622021000100209
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/1806-908820210000009
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade de Investigações Florestais
publisher.none.fl_str_mv Sociedade de Investigações Florestais
dc.source.none.fl_str_mv Revista Árvore v.45 2021
reponame:Revista Árvore (Online)
instname:Universidade Federal de Viçosa (UFV)
instacron:SIF
instname_str Universidade Federal de Viçosa (UFV)
instacron_str SIF
institution SIF
reponame_str Revista Árvore (Online)
collection Revista Árvore (Online)
repository.name.fl_str_mv Revista Árvore (Online) - Universidade Federal de Viçosa (UFV)
repository.mail.fl_str_mv ||r.arvore@ufv.br
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