PERMEABILIZATION, CELL WALL ULTRASTRUCTURE, AND GERMINATION OF BASIDIOSPORES OF THE ECTOMYCORRHIZAL FUNGUS Pisolithus microcarpus TREATED WITH DIFFERENT COMMERCIAL BRANDS OF BLEACH

Detalhes bibliográficos
Autor(a) principal: Silvério, Merielle Angélica Martines [UNESP]
Data de Publicação: 2021
Outros Autores: Nascimento, Jaqueline Maria Do, Serrão, José Eduardo, Tótola, Marcos Rogério, Costa, Maurício Dutra
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/1806-908820210000009
http://hdl.handle.net/11449/211218
Resumo: Basidiospores of the ectomycorrhizal fungus Pisolithus microcarpus have an impermeable cell wall, a characteristic that is possibly related to the low germination percentages of these propagules, which makes it difficult to obtain monokaryons and use these spores in inoculants. The objective of this study was to evaluate the effect of different concentrations of commercial bleach on the permeabilization of P. microcarpus basidiospores and to analyze the alterations caused in the cell wall ultrastructure and the viability and germination capacity of these propagules. Fungal basidiospores were collected in eucalyptus plantations and permeabilized using different bleach concentrations and exposure times. The basidiospores were then analyzed by scanning and transmission electron microscopy. The percentage of permeabilized basidiospores varied with the commercial brand, bleach concentration, and exposure time. Basidiospores of different basidiocarps differed in susceptibility to permeabilization treatment with bleach. Changes in the ultrastructure of permeabilized basidiospores were observed at bleach concentrations of 15 and 50 % for an exposure time of 40 s, with surface changes and loss of the spicules of the outermost layer of the wall. After permeabilization with 5 % bleach for 40 s, 80 % of the permeabilized spores were viable, resulting in the production of fungal colonies after 15 days of incubation of these propagules in the presence of Corymbia citriodora. However, the germination percentage obtained, 0.001 %, was similar to that of non-permeabilized basidiospores, indicating that other factors, besides cell wall permeability, are determinant for the germination process.
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spelling PERMEABILIZATION, CELL WALL ULTRASTRUCTURE, AND GERMINATION OF BASIDIOSPORES OF THE ECTOMYCORRHIZAL FUNGUS Pisolithus microcarpus TREATED WITH DIFFERENT COMMERCIAL BRANDS OF BLEACHPERMEABILIZAÇÃO, ULTRAESTRUTURA DA PAREDE CELULAR E GERMINAÇÃO DE BASIDIÓSPOROS DO FUNGO EcTOMICORRÍZICO Pisolithus microcarpus TRATADOS COM DIFERENTES MARCAS COMERCIAIS DE ÁGUA SANITÁRIAEctomycorrhizaInoculantEucalyptusEctomicorrizaInoculanteEucalyptusBasidiospores of the ectomycorrhizal fungus Pisolithus microcarpus have an impermeable cell wall, a characteristic that is possibly related to the low germination percentages of these propagules, which makes it difficult to obtain monokaryons and use these spores in inoculants. The objective of this study was to evaluate the effect of different concentrations of commercial bleach on the permeabilization of P. microcarpus basidiospores and to analyze the alterations caused in the cell wall ultrastructure and the viability and germination capacity of these propagules. Fungal basidiospores were collected in eucalyptus plantations and permeabilized using different bleach concentrations and exposure times. The basidiospores were then analyzed by scanning and transmission electron microscopy. The percentage of permeabilized basidiospores varied with the commercial brand, bleach concentration, and exposure time. Basidiospores of different basidiocarps differed in susceptibility to permeabilization treatment with bleach. Changes in the ultrastructure of permeabilized basidiospores were observed at bleach concentrations of 15 and 50 % for an exposure time of 40 s, with surface changes and loss of the spicules of the outermost layer of the wall. After permeabilization with 5 % bleach for 40 s, 80 % of the permeabilized spores were viable, resulting in the production of fungal colonies after 15 days of incubation of these propagules in the presence of Corymbia citriodora. However, the germination percentage obtained, 0.001 %, was similar to that of non-permeabilized basidiospores, indicating that other factors, besides cell wall permeability, are determinant for the germination process.Basidiósporos do fungo ectomicorrízico Pisolithus microcarpus apresentam parede celular impermeável, característica possivelmente relacionada às baixas porcentagens de germinação desses propágulos, o que dificulta a obtenção de monocários e a utilização desses esporos em inoculantes. O objetivo deste estudo foi avaliar o efeito de diferentes concentrações de água sanitária comercial na permeabilização dos basidiósporos de P. microcarpus e analisar as alterações causadas na ultraestrutura da parede celular, na viabilidade e na capacidade germinativa desses propágulos. Basidiósporos fúngicos foram coletados em plantações de eucalipto e permeabilizados utilizando-se diferentes concentrações de água sanitária e tempos de exposição. Os basidiósporos foram então analisados por microscopia eletrônica de varredura e de transmissão. A porcentagem de basidiósporos permeabilizados variou com a marca comercial, concentração de água sanitária e o tempo de exposição. Basidiósporos de diferentes basidiocarpos diferiram na suscetibilidade ao tratamento de permeabilização com água sanitária. Alterações da ultraestrutura dos basidiósporos permeabilizados foram observadas nas concentrações de 15 e 50 % de água sanitária pelo tempo de exposição de 40 s, com mudanças de superfície e perda das espículas da camada mais externa da parede. Após a permeabilização com água sanitária a 5 % por 40 s, 80 % dos esporos permeabilizados encontravam-se viáveis, resultando na produção de colônias fúngicas após 15 dias de incubação desses propágulos na presença de Corymbia citriodora. No entanto, a porcentagem de germinação obtida: 0,001%, foi semelhante à de basidiósporos não-permeabilizados, indicando que outros fatores, além da permeabilidade da parede celular, são determinantes para o processo de germinação.Universidade Estadual Paulista Júlio de Mesquita Filho, Programa de Pós-Graduação em Educação Para a CiênciaUniversidade Federal de Viçosa, Programa de Pós-Graduação em Microbiologia AgrícolaUniversidade Federal de Viçosa, Departamento de Biologia GeralUniversidade Federal de Viçosa, Departamento de MicrobiologiaUniversidade Estadual Paulista Júlio de Mesquita Filho, Programa de Pós-Graduação em Educação Para a CiênciaSociedade de Investigações FlorestaisUniversidade Estadual Paulista (Unesp)Universidade Federal de ViçosaSilvério, Merielle Angélica Martines [UNESP]Nascimento, Jaqueline Maria DoSerrão, José EduardoTótola, Marcos RogérioCosta, Maurício Dutra2021-07-14T10:21:04Z2021-07-14T10:21:04Z2021-07-02info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article-application/pdfhttp://dx.doi.org/10.1590/1806-908820210000009Revista Árvore. Sociedade de Investigações Florestais, v. 45, p. -, 2021.0100-67621806-9088http://hdl.handle.net/11449/21121810.1590/1806-908820210000009S0100-67622021000100209S0100-67622021000100209.pdfSciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengRevista Árvoreinfo:eu-repo/semantics/openAccess2024-01-04T06:23:12Zoai:repositorio.unesp.br:11449/211218Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T22:04:49.755525Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv PERMEABILIZATION, CELL WALL ULTRASTRUCTURE, AND GERMINATION OF BASIDIOSPORES OF THE ECTOMYCORRHIZAL FUNGUS Pisolithus microcarpus TREATED WITH DIFFERENT COMMERCIAL BRANDS OF BLEACH
PERMEABILIZAÇÃO, ULTRAESTRUTURA DA PAREDE CELULAR E GERMINAÇÃO DE BASIDIÓSPOROS DO FUNGO EcTOMICORRÍZICO Pisolithus microcarpus TRATADOS COM DIFERENTES MARCAS COMERCIAIS DE ÁGUA SANITÁRIA
title PERMEABILIZATION, CELL WALL ULTRASTRUCTURE, AND GERMINATION OF BASIDIOSPORES OF THE ECTOMYCORRHIZAL FUNGUS Pisolithus microcarpus TREATED WITH DIFFERENT COMMERCIAL BRANDS OF BLEACH
spellingShingle PERMEABILIZATION, CELL WALL ULTRASTRUCTURE, AND GERMINATION OF BASIDIOSPORES OF THE ECTOMYCORRHIZAL FUNGUS Pisolithus microcarpus TREATED WITH DIFFERENT COMMERCIAL BRANDS OF BLEACH
Silvério, Merielle Angélica Martines [UNESP]
Ectomycorrhiza
Inoculant
Eucalyptus
Ectomicorriza
Inoculante
Eucalyptus
title_short PERMEABILIZATION, CELL WALL ULTRASTRUCTURE, AND GERMINATION OF BASIDIOSPORES OF THE ECTOMYCORRHIZAL FUNGUS Pisolithus microcarpus TREATED WITH DIFFERENT COMMERCIAL BRANDS OF BLEACH
title_full PERMEABILIZATION, CELL WALL ULTRASTRUCTURE, AND GERMINATION OF BASIDIOSPORES OF THE ECTOMYCORRHIZAL FUNGUS Pisolithus microcarpus TREATED WITH DIFFERENT COMMERCIAL BRANDS OF BLEACH
title_fullStr PERMEABILIZATION, CELL WALL ULTRASTRUCTURE, AND GERMINATION OF BASIDIOSPORES OF THE ECTOMYCORRHIZAL FUNGUS Pisolithus microcarpus TREATED WITH DIFFERENT COMMERCIAL BRANDS OF BLEACH
title_full_unstemmed PERMEABILIZATION, CELL WALL ULTRASTRUCTURE, AND GERMINATION OF BASIDIOSPORES OF THE ECTOMYCORRHIZAL FUNGUS Pisolithus microcarpus TREATED WITH DIFFERENT COMMERCIAL BRANDS OF BLEACH
title_sort PERMEABILIZATION, CELL WALL ULTRASTRUCTURE, AND GERMINATION OF BASIDIOSPORES OF THE ECTOMYCORRHIZAL FUNGUS Pisolithus microcarpus TREATED WITH DIFFERENT COMMERCIAL BRANDS OF BLEACH
author Silvério, Merielle Angélica Martines [UNESP]
author_facet Silvério, Merielle Angélica Martines [UNESP]
Nascimento, Jaqueline Maria Do
Serrão, José Eduardo
Tótola, Marcos Rogério
Costa, Maurício Dutra
author_role author
author2 Nascimento, Jaqueline Maria Do
Serrão, José Eduardo
Tótola, Marcos Rogério
Costa, Maurício Dutra
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Universidade Federal de Viçosa
dc.contributor.author.fl_str_mv Silvério, Merielle Angélica Martines [UNESP]
Nascimento, Jaqueline Maria Do
Serrão, José Eduardo
Tótola, Marcos Rogério
Costa, Maurício Dutra
dc.subject.por.fl_str_mv Ectomycorrhiza
Inoculant
Eucalyptus
Ectomicorriza
Inoculante
Eucalyptus
topic Ectomycorrhiza
Inoculant
Eucalyptus
Ectomicorriza
Inoculante
Eucalyptus
description Basidiospores of the ectomycorrhizal fungus Pisolithus microcarpus have an impermeable cell wall, a characteristic that is possibly related to the low germination percentages of these propagules, which makes it difficult to obtain monokaryons and use these spores in inoculants. The objective of this study was to evaluate the effect of different concentrations of commercial bleach on the permeabilization of P. microcarpus basidiospores and to analyze the alterations caused in the cell wall ultrastructure and the viability and germination capacity of these propagules. Fungal basidiospores were collected in eucalyptus plantations and permeabilized using different bleach concentrations and exposure times. The basidiospores were then analyzed by scanning and transmission electron microscopy. The percentage of permeabilized basidiospores varied with the commercial brand, bleach concentration, and exposure time. Basidiospores of different basidiocarps differed in susceptibility to permeabilization treatment with bleach. Changes in the ultrastructure of permeabilized basidiospores were observed at bleach concentrations of 15 and 50 % for an exposure time of 40 s, with surface changes and loss of the spicules of the outermost layer of the wall. After permeabilization with 5 % bleach for 40 s, 80 % of the permeabilized spores were viable, resulting in the production of fungal colonies after 15 days of incubation of these propagules in the presence of Corymbia citriodora. However, the germination percentage obtained, 0.001 %, was similar to that of non-permeabilized basidiospores, indicating that other factors, besides cell wall permeability, are determinant for the germination process.
publishDate 2021
dc.date.none.fl_str_mv 2021-07-14T10:21:04Z
2021-07-14T10:21:04Z
2021-07-02
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/1806-908820210000009
Revista Árvore. Sociedade de Investigações Florestais, v. 45, p. -, 2021.
0100-6762
1806-9088
http://hdl.handle.net/11449/211218
10.1590/1806-908820210000009
S0100-67622021000100209
S0100-67622021000100209.pdf
url http://dx.doi.org/10.1590/1806-908820210000009
http://hdl.handle.net/11449/211218
identifier_str_mv Revista Árvore. Sociedade de Investigações Florestais, v. 45, p. -, 2021.
0100-6762
1806-9088
10.1590/1806-908820210000009
S0100-67622021000100209
S0100-67622021000100209.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Revista Árvore
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv -
application/pdf
dc.publisher.none.fl_str_mv Sociedade de Investigações Florestais
publisher.none.fl_str_mv Sociedade de Investigações Florestais
dc.source.none.fl_str_mv SciELO
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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