In vitro multiplication of Tabernaemontana fuchsiaefolia L. (Apocynaceae)
Autor(a) principal: | |
---|---|
Data de Publicação: | 2003 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista Árvore (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-67622003000400001 |
Resumo: | This study describes a simple and promising for in vitro multiplication of Tabernaemontana fuchsiaefolia, a species abundantly found in southern Brazil utilized for medicinal purposes and as a source of compounds that may be used to develop new synthetic drugs. Apical and hypocotyl explants were cultured in MS medium containing different concentrations of the cytokinins benzylaminopurine (BA) and 6-furfurylaminopurine (kinetin), supplemented with phloroglucinol (1, 3, 5-hydroxybenzene) to stimulate growth and shoot proliferation. Cytokinin added to the culture media positively influenced the micropropagation of T. fuchsiaefolia.and kinetin induced more shoots per explant than BA cytokinin. A favorable effect of phloroglucinol on apical and lateral buds from hypocotyls was also achieved in medium containing no kinetin or in all kinetin concentrations tested. Short pulses of auxin 3-indolebutyric acid (IBA) 5.0 mg/l resulted in satisfactory rooting in apical microcuttings. The addition of phloroglucinol to MS medium induced rhizogenesis in 29% of the nodal segments transferred to MS medium in the absence of IBA and in 50% of the nodal segments transferred to MS medium containing 0.5 mg/l IBA and in nodal segments previously submitted to short pulses of IBA. |
id |
SIF-1_bca1cb8edd63c19b1eabb87b63c6da4f |
---|---|
oai_identifier_str |
oai:scielo:S0100-67622003000400001 |
network_acronym_str |
SIF-1 |
network_name_str |
Revista Árvore (Online) |
repository_id_str |
|
spelling |
In vitro multiplication of Tabernaemontana fuchsiaefolia L. (Apocynaceae)micropropagationTabernaemontana fuchsiaefoliaApocynaceaetissue cultureThis study describes a simple and promising for in vitro multiplication of Tabernaemontana fuchsiaefolia, a species abundantly found in southern Brazil utilized for medicinal purposes and as a source of compounds that may be used to develop new synthetic drugs. Apical and hypocotyl explants were cultured in MS medium containing different concentrations of the cytokinins benzylaminopurine (BA) and 6-furfurylaminopurine (kinetin), supplemented with phloroglucinol (1, 3, 5-hydroxybenzene) to stimulate growth and shoot proliferation. Cytokinin added to the culture media positively influenced the micropropagation of T. fuchsiaefolia.and kinetin induced more shoots per explant than BA cytokinin. A favorable effect of phloroglucinol on apical and lateral buds from hypocotyls was also achieved in medium containing no kinetin or in all kinetin concentrations tested. Short pulses of auxin 3-indolebutyric acid (IBA) 5.0 mg/l resulted in satisfactory rooting in apical microcuttings. The addition of phloroglucinol to MS medium induced rhizogenesis in 29% of the nodal segments transferred to MS medium in the absence of IBA and in 50% of the nodal segments transferred to MS medium containing 0.5 mg/l IBA and in nodal segments previously submitted to short pulses of IBA.Sociedade de Investigações Florestais2003-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-67622003000400001Revista Árvore v.27 n.4 2003reponame:Revista Árvore (Online)instname:Universidade Federal de Viçosa (UFV)instacron:SIF10.1590/S0100-67622003000400001info:eu-repo/semantics/openAccessOliveira,Arildo José Braz deCarvalho,Vanda Marilza deFerreira,AlexandreSato,Fernando Y.Machado,Maria de Fátima Pires da Silvaeng2003-12-23T00:00:00Zoai:scielo:S0100-67622003000400001Revistahttp://www.scielo.br/revistas/rarv/iaboutj.htmPUBhttps://old.scielo.br/oai/scielo-oai.php||r.arvore@ufv.br1806-90880100-6762opendoar:2003-12-23T00:00Revista Árvore (Online) - Universidade Federal de Viçosa (UFV)false |
dc.title.none.fl_str_mv |
In vitro multiplication of Tabernaemontana fuchsiaefolia L. (Apocynaceae) |
title |
In vitro multiplication of Tabernaemontana fuchsiaefolia L. (Apocynaceae) |
spellingShingle |
In vitro multiplication of Tabernaemontana fuchsiaefolia L. (Apocynaceae) Oliveira,Arildo José Braz de micropropagation Tabernaemontana fuchsiaefolia Apocynaceae tissue culture |
title_short |
In vitro multiplication of Tabernaemontana fuchsiaefolia L. (Apocynaceae) |
title_full |
In vitro multiplication of Tabernaemontana fuchsiaefolia L. (Apocynaceae) |
title_fullStr |
In vitro multiplication of Tabernaemontana fuchsiaefolia L. (Apocynaceae) |
title_full_unstemmed |
In vitro multiplication of Tabernaemontana fuchsiaefolia L. (Apocynaceae) |
title_sort |
In vitro multiplication of Tabernaemontana fuchsiaefolia L. (Apocynaceae) |
author |
Oliveira,Arildo José Braz de |
author_facet |
Oliveira,Arildo José Braz de Carvalho,Vanda Marilza de Ferreira,Alexandre Sato,Fernando Y. Machado,Maria de Fátima Pires da Silva |
author_role |
author |
author2 |
Carvalho,Vanda Marilza de Ferreira,Alexandre Sato,Fernando Y. Machado,Maria de Fátima Pires da Silva |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Oliveira,Arildo José Braz de Carvalho,Vanda Marilza de Ferreira,Alexandre Sato,Fernando Y. Machado,Maria de Fátima Pires da Silva |
dc.subject.por.fl_str_mv |
micropropagation Tabernaemontana fuchsiaefolia Apocynaceae tissue culture |
topic |
micropropagation Tabernaemontana fuchsiaefolia Apocynaceae tissue culture |
description |
This study describes a simple and promising for in vitro multiplication of Tabernaemontana fuchsiaefolia, a species abundantly found in southern Brazil utilized for medicinal purposes and as a source of compounds that may be used to develop new synthetic drugs. Apical and hypocotyl explants were cultured in MS medium containing different concentrations of the cytokinins benzylaminopurine (BA) and 6-furfurylaminopurine (kinetin), supplemented with phloroglucinol (1, 3, 5-hydroxybenzene) to stimulate growth and shoot proliferation. Cytokinin added to the culture media positively influenced the micropropagation of T. fuchsiaefolia.and kinetin induced more shoots per explant than BA cytokinin. A favorable effect of phloroglucinol on apical and lateral buds from hypocotyls was also achieved in medium containing no kinetin or in all kinetin concentrations tested. Short pulses of auxin 3-indolebutyric acid (IBA) 5.0 mg/l resulted in satisfactory rooting in apical microcuttings. The addition of phloroglucinol to MS medium induced rhizogenesis in 29% of the nodal segments transferred to MS medium in the absence of IBA and in 50% of the nodal segments transferred to MS medium containing 0.5 mg/l IBA and in nodal segments previously submitted to short pulses of IBA. |
publishDate |
2003 |
dc.date.none.fl_str_mv |
2003-08-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-67622003000400001 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-67622003000400001 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0100-67622003000400001 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade de Investigações Florestais |
publisher.none.fl_str_mv |
Sociedade de Investigações Florestais |
dc.source.none.fl_str_mv |
Revista Árvore v.27 n.4 2003 reponame:Revista Árvore (Online) instname:Universidade Federal de Viçosa (UFV) instacron:SIF |
instname_str |
Universidade Federal de Viçosa (UFV) |
instacron_str |
SIF |
institution |
SIF |
reponame_str |
Revista Árvore (Online) |
collection |
Revista Árvore (Online) |
repository.name.fl_str_mv |
Revista Árvore (Online) - Universidade Federal de Viçosa (UFV) |
repository.mail.fl_str_mv |
||r.arvore@ufv.br |
_version_ |
1750317995590156288 |