In vitro multiplication of Tabernaemontana fuchsiaefolia L. (Apocynaceae)

• Autor(a) principal: Oliveira,Arildo José Braz de
• Data de Publicação: 2003
• Outros Autores: Carvalho,Vanda Marilza de, Ferreira,Alexandre, Sato,Fernando Y., Machado,Maria de Fátima Pires da Silva
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Revista Árvore (Online)
• Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-67622003000400001
• topic: micropropagation; Tabernaemontana fuchsiaefolia; Apocynaceae; tissue culture

This study describes a simple and promising for in vitro multiplication of Tabernaemontana fuchsiaefolia, a species abundantly found in southern Brazil utilized for medicinal purposes and as a source of compounds that may be used to develop new synthetic drugs. Apical and hypocotyl explants were cultured in MS medium containing different concentrations of the cytokinins benzylaminopurine (BA) and 6-furfurylaminopurine (kinetin), supplemented with phloroglucinol (1, 3, 5-hydroxybenzene) to stimulate growth and shoot proliferation. Cytokinin added to the culture media positively influenced the micropropagation of T. fuchsiaefolia.and kinetin induced more shoots per explant than BA cytokinin. A favorable effect of phloroglucinol on apical and lateral buds from hypocotyls was also achieved in medium containing no kinetin or in all kinetin concentrations tested. Short pulses of auxin 3-indolebutyric acid (IBA) 5.0 mg/l resulted in satisfactory rooting in apical microcuttings. The addition of phloroglucinol to MS medium induced rhizogenesis in 29% of the nodal segments transferred to MS medium in the absence of IBA and in 50% of the nodal segments transferred to MS medium containing 0.5 mg/l IBA and in nodal segments previously submitted to short pulses of IBA.