CYTOKININ AND FLASK SEALING AFFECT SHOOT PROLIFERATION AND In Vitro DEVELOPMENT OF Jacaranda cuspidifolia MART. MICROCUTTINGS
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista Árvore (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-67622022000100238 |
Resumo: | ABSTRACT Plant growth regulators (PGRs) and types of flasks sealing that allow gas exchange can favor the development of in vitro plant propagation systems. The objective of the present study was to evaluate the eff ects of cytokinin supplementation on the induction of shoot proliferation and the influence of gas exchange on the in vitro development of Jacaranda cuspidifolia Mart microcuttings. Nodal segments were cultured in medium supplemented with diff erent concentrations (0.25; 0.5; 1.0 and 2.0 mg L-1) of 6-benzyladenine (BA). In the control treatment, there was no addition of PGRs. After 30 days of culture, the microcuttings were isolated and transferred to a rooting medium supplemented with 1.0 mg L-1 indole-3-butyric acid. At this stage, the culture fl asks were sealed with rigid polypropylene lids with a porous membrane (1 M; 21 μL L-1 s-1 CO2) or with no membrane (0 M; 14 μL L-1 s-1 CO2). Cytokinin supplementation induced the activation of axillary buds of J. cuspidifolia. The highest numbers of shoots were observed in explants cultivated in the presence of 0.5–1.0 mg L-1 BA. The microcuttings kept in flasks with a higher level of gas exchange (1M) had a higher percentage of rooting and greater root and shoot lengths. Microscopic analysis showed a greater differentiation of leaf tissues in plants kept in flasks with a higher level of gas exchange (1M). These plants also showed greater mesophyll thickness and, consequently, greater leaf blade thickness. The results provide new information for establishing an efficient in vitro propagation system for J. cuspidifolia. |
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CYTOKININ AND FLASK SEALING AFFECT SHOOT PROLIFERATION AND In Vitro DEVELOPMENT OF Jacaranda cuspidifolia MART. MICROCUTTINGSCytokininGas ExchangeMicropropagationABSTRACT Plant growth regulators (PGRs) and types of flasks sealing that allow gas exchange can favor the development of in vitro plant propagation systems. The objective of the present study was to evaluate the eff ects of cytokinin supplementation on the induction of shoot proliferation and the influence of gas exchange on the in vitro development of Jacaranda cuspidifolia Mart microcuttings. Nodal segments were cultured in medium supplemented with diff erent concentrations (0.25; 0.5; 1.0 and 2.0 mg L-1) of 6-benzyladenine (BA). In the control treatment, there was no addition of PGRs. After 30 days of culture, the microcuttings were isolated and transferred to a rooting medium supplemented with 1.0 mg L-1 indole-3-butyric acid. At this stage, the culture fl asks were sealed with rigid polypropylene lids with a porous membrane (1 M; 21 μL L-1 s-1 CO2) or with no membrane (0 M; 14 μL L-1 s-1 CO2). Cytokinin supplementation induced the activation of axillary buds of J. cuspidifolia. The highest numbers of shoots were observed in explants cultivated in the presence of 0.5–1.0 mg L-1 BA. The microcuttings kept in flasks with a higher level of gas exchange (1M) had a higher percentage of rooting and greater root and shoot lengths. Microscopic analysis showed a greater differentiation of leaf tissues in plants kept in flasks with a higher level of gas exchange (1M). These plants also showed greater mesophyll thickness and, consequently, greater leaf blade thickness. The results provide new information for establishing an efficient in vitro propagation system for J. cuspidifolia.Sociedade de Investigações Florestais2022-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-67622022000100238Revista Árvore v.46 2022reponame:Revista Árvore (Online)instname:Universidade Federal de Viçosa (UFV)instacron:SIF10.1590/1806-908820220000033info:eu-repo/semantics/openAccessFerreira,Deise Kelle BarbosaDias,Lana Laene LimaSilva,Lázara Aline SimõesNetto,Antônio Paulino da CostaKuster,Vinícius CoelhoRocha,Diego Ismaeleng2022-10-14T00:00:00Zoai:scielo:S0100-67622022000100238Revistahttp://www.scielo.br/revistas/rarv/iaboutj.htmPUBhttps://old.scielo.br/oai/scielo-oai.php||r.arvore@ufv.br1806-90880100-6762opendoar:2022-10-14T00:00Revista Árvore (Online) - Universidade Federal de Viçosa (UFV)false |
dc.title.none.fl_str_mv |
CYTOKININ AND FLASK SEALING AFFECT SHOOT PROLIFERATION AND In Vitro DEVELOPMENT OF Jacaranda cuspidifolia MART. MICROCUTTINGS |
title |
CYTOKININ AND FLASK SEALING AFFECT SHOOT PROLIFERATION AND In Vitro DEVELOPMENT OF Jacaranda cuspidifolia MART. MICROCUTTINGS |
spellingShingle |
CYTOKININ AND FLASK SEALING AFFECT SHOOT PROLIFERATION AND In Vitro DEVELOPMENT OF Jacaranda cuspidifolia MART. MICROCUTTINGS Ferreira,Deise Kelle Barbosa Cytokinin Gas Exchange Micropropagation |
title_short |
CYTOKININ AND FLASK SEALING AFFECT SHOOT PROLIFERATION AND In Vitro DEVELOPMENT OF Jacaranda cuspidifolia MART. MICROCUTTINGS |
title_full |
CYTOKININ AND FLASK SEALING AFFECT SHOOT PROLIFERATION AND In Vitro DEVELOPMENT OF Jacaranda cuspidifolia MART. MICROCUTTINGS |
title_fullStr |
CYTOKININ AND FLASK SEALING AFFECT SHOOT PROLIFERATION AND In Vitro DEVELOPMENT OF Jacaranda cuspidifolia MART. MICROCUTTINGS |
title_full_unstemmed |
CYTOKININ AND FLASK SEALING AFFECT SHOOT PROLIFERATION AND In Vitro DEVELOPMENT OF Jacaranda cuspidifolia MART. MICROCUTTINGS |
title_sort |
CYTOKININ AND FLASK SEALING AFFECT SHOOT PROLIFERATION AND In Vitro DEVELOPMENT OF Jacaranda cuspidifolia MART. MICROCUTTINGS |
author |
Ferreira,Deise Kelle Barbosa |
author_facet |
Ferreira,Deise Kelle Barbosa Dias,Lana Laene Lima Silva,Lázara Aline Simões Netto,Antônio Paulino da Costa Kuster,Vinícius Coelho Rocha,Diego Ismael |
author_role |
author |
author2 |
Dias,Lana Laene Lima Silva,Lázara Aline Simões Netto,Antônio Paulino da Costa Kuster,Vinícius Coelho Rocha,Diego Ismael |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Ferreira,Deise Kelle Barbosa Dias,Lana Laene Lima Silva,Lázara Aline Simões Netto,Antônio Paulino da Costa Kuster,Vinícius Coelho Rocha,Diego Ismael |
dc.subject.por.fl_str_mv |
Cytokinin Gas Exchange Micropropagation |
topic |
Cytokinin Gas Exchange Micropropagation |
description |
ABSTRACT Plant growth regulators (PGRs) and types of flasks sealing that allow gas exchange can favor the development of in vitro plant propagation systems. The objective of the present study was to evaluate the eff ects of cytokinin supplementation on the induction of shoot proliferation and the influence of gas exchange on the in vitro development of Jacaranda cuspidifolia Mart microcuttings. Nodal segments were cultured in medium supplemented with diff erent concentrations (0.25; 0.5; 1.0 and 2.0 mg L-1) of 6-benzyladenine (BA). In the control treatment, there was no addition of PGRs. After 30 days of culture, the microcuttings were isolated and transferred to a rooting medium supplemented with 1.0 mg L-1 indole-3-butyric acid. At this stage, the culture fl asks were sealed with rigid polypropylene lids with a porous membrane (1 M; 21 μL L-1 s-1 CO2) or with no membrane (0 M; 14 μL L-1 s-1 CO2). Cytokinin supplementation induced the activation of axillary buds of J. cuspidifolia. The highest numbers of shoots were observed in explants cultivated in the presence of 0.5–1.0 mg L-1 BA. The microcuttings kept in flasks with a higher level of gas exchange (1M) had a higher percentage of rooting and greater root and shoot lengths. Microscopic analysis showed a greater differentiation of leaf tissues in plants kept in flasks with a higher level of gas exchange (1M). These plants also showed greater mesophyll thickness and, consequently, greater leaf blade thickness. The results provide new information for establishing an efficient in vitro propagation system for J. cuspidifolia. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-67622022000100238 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-67622022000100238 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/1806-908820220000033 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade de Investigações Florestais |
publisher.none.fl_str_mv |
Sociedade de Investigações Florestais |
dc.source.none.fl_str_mv |
Revista Árvore v.46 2022 reponame:Revista Árvore (Online) instname:Universidade Federal de Viçosa (UFV) instacron:SIF |
instname_str |
Universidade Federal de Viçosa (UFV) |
instacron_str |
SIF |
institution |
SIF |
reponame_str |
Revista Árvore (Online) |
collection |
Revista Árvore (Online) |
repository.name.fl_str_mv |
Revista Árvore (Online) - Universidade Federal de Viçosa (UFV) |
repository.mail.fl_str_mv |
||r.arvore@ufv.br |
_version_ |
1750318003691454464 |