EFFECT OF BAP (6-BENZYLAMINOPURINE) ON SHOOT INDUCTION IN EXPLANTS OF BRAZILWOOD

Detalhes bibliográficos
Autor(a) principal: Aragão, Ana Katarina Oliveira
Data de Publicação: 2015
Outros Autores: Aloufa, Magdi Ahmed Ibrahim, Costa, Igor do Amaral
Tipo de documento: Artigo
Idioma: por
Título da fonte: Cerne (Online)
Texto Completo: https://cerne.ufla.br/site/index.php/CERNE/article/view/53
Resumo: The Brazilian Atlantic forest has been subjected to intense degradation, with only about 7% to 8% of its original area remaining today. This situation has raised concerns over the conservation of species threatened with extinction. In all, 276 tree and bush species are under threat, out of which this study chose to evaluate alternatives for protecting brazilwood ‘Pau-Brasil’ (Caesalpinia echinata Lam.). Most studies performed so far on this subject either evaluate the effect of cytokinins on induction of callogenesis or focus on improving cryopreservation methodologies. In an attempt to expand knowledge about biotechnological techniques enabling conservation of C. echinata, this work evaluated the effect of 6-benzylaminopurine (BAP) and explant type on induction of shoots in brazilwood. To attain that, explants were inoculated into basic MS medium and into MS medium supplemented with 2.5 μM, 3.5 μM and 4.5 μM of BAP, and kept in a growth room for 40 days under controlled photoperiod and temperature conditions. A 2x4 factorial design was adopted, with three replicates. Analyzed variables included shoot percentage, callogenesis and oxidations, and means were compared by the Tukey test at the 5% probability level. Results showed a signifi cant infl uence of BAP only on shoot induction, and of explant type on that variable and on other variables too. It was concluded that, under in vitro conditions, the nodal type of explant is more responsive to BAP action and that 2.5 μM is the recommended concentration for shoot induction in brazilwood. 
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spelling EFFECT OF BAP (6-BENZYLAMINOPURINE) ON SHOOT INDUCTION IN EXPLANTS OF BRAZILWOODIn vitro micropropagationcytokinincallogenesis and oxidationsThe Brazilian Atlantic forest has been subjected to intense degradation, with only about 7% to 8% of its original area remaining today. This situation has raised concerns over the conservation of species threatened with extinction. In all, 276 tree and bush species are under threat, out of which this study chose to evaluate alternatives for protecting brazilwood ‘Pau-Brasil’ (Caesalpinia echinata Lam.). Most studies performed so far on this subject either evaluate the effect of cytokinins on induction of callogenesis or focus on improving cryopreservation methodologies. In an attempt to expand knowledge about biotechnological techniques enabling conservation of C. echinata, this work evaluated the effect of 6-benzylaminopurine (BAP) and explant type on induction of shoots in brazilwood. To attain that, explants were inoculated into basic MS medium and into MS medium supplemented with 2.5 μM, 3.5 μM and 4.5 μM of BAP, and kept in a growth room for 40 days under controlled photoperiod and temperature conditions. A 2x4 factorial design was adopted, with three replicates. Analyzed variables included shoot percentage, callogenesis and oxidations, and means were compared by the Tukey test at the 5% probability level. Results showed a signifi cant infl uence of BAP only on shoot induction, and of explant type on that variable and on other variables too. It was concluded that, under in vitro conditions, the nodal type of explant is more responsive to BAP action and that 2.5 μM is the recommended concentration for shoot induction in brazilwood. CERNECERNE2015-05-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://cerne.ufla.br/site/index.php/CERNE/article/view/53CERNE; Vol. 17 No. 3 (2011); 339-345CERNE; v. 17 n. 3 (2011); 339-3452317-63420104-7760reponame:Cerne (Online)instname:Universidade Federal de Lavras (UFLA)instacron:UFLAporhttps://cerne.ufla.br/site/index.php/CERNE/article/view/53/45Copyright (c) 2015 Ana Katarina Oliveira Aragão, Magdi Ahmed Ibrahim Aloufa, Igor do Amaral Costainfo:eu-repo/semantics/openAccessAragão, Ana Katarina OliveiraAloufa, Magdi Ahmed IbrahimCosta, Igor do Amaral2015-05-12T11:04:19Zoai:cerne.ufla.br:article/53Revistahttps://cerne.ufla.br/site/index.php/CERNEPUBhttps://cerne.ufla.br/site/index.php/CERNE/oaicerne@dcf.ufla.br||cerne@dcf.ufla.br2317-63420104-7760opendoar:2024-05-21T19:53:28.196132Cerne (Online) - Universidade Federal de Lavras (UFLA)true
dc.title.none.fl_str_mv EFFECT OF BAP (6-BENZYLAMINOPURINE) ON SHOOT INDUCTION IN EXPLANTS OF BRAZILWOOD
title EFFECT OF BAP (6-BENZYLAMINOPURINE) ON SHOOT INDUCTION IN EXPLANTS OF BRAZILWOOD
spellingShingle EFFECT OF BAP (6-BENZYLAMINOPURINE) ON SHOOT INDUCTION IN EXPLANTS OF BRAZILWOOD
Aragão, Ana Katarina Oliveira
In vitro micropropagation
cytokinin
callogenesis and oxidations
title_short EFFECT OF BAP (6-BENZYLAMINOPURINE) ON SHOOT INDUCTION IN EXPLANTS OF BRAZILWOOD
title_full EFFECT OF BAP (6-BENZYLAMINOPURINE) ON SHOOT INDUCTION IN EXPLANTS OF BRAZILWOOD
title_fullStr EFFECT OF BAP (6-BENZYLAMINOPURINE) ON SHOOT INDUCTION IN EXPLANTS OF BRAZILWOOD
title_full_unstemmed EFFECT OF BAP (6-BENZYLAMINOPURINE) ON SHOOT INDUCTION IN EXPLANTS OF BRAZILWOOD
title_sort EFFECT OF BAP (6-BENZYLAMINOPURINE) ON SHOOT INDUCTION IN EXPLANTS OF BRAZILWOOD
author Aragão, Ana Katarina Oliveira
author_facet Aragão, Ana Katarina Oliveira
Aloufa, Magdi Ahmed Ibrahim
Costa, Igor do Amaral
author_role author
author2 Aloufa, Magdi Ahmed Ibrahim
Costa, Igor do Amaral
author2_role author
author
dc.contributor.author.fl_str_mv Aragão, Ana Katarina Oliveira
Aloufa, Magdi Ahmed Ibrahim
Costa, Igor do Amaral
dc.subject.por.fl_str_mv In vitro micropropagation
cytokinin
callogenesis and oxidations
topic In vitro micropropagation
cytokinin
callogenesis and oxidations
description The Brazilian Atlantic forest has been subjected to intense degradation, with only about 7% to 8% of its original area remaining today. This situation has raised concerns over the conservation of species threatened with extinction. In all, 276 tree and bush species are under threat, out of which this study chose to evaluate alternatives for protecting brazilwood ‘Pau-Brasil’ (Caesalpinia echinata Lam.). Most studies performed so far on this subject either evaluate the effect of cytokinins on induction of callogenesis or focus on improving cryopreservation methodologies. In an attempt to expand knowledge about biotechnological techniques enabling conservation of C. echinata, this work evaluated the effect of 6-benzylaminopurine (BAP) and explant type on induction of shoots in brazilwood. To attain that, explants were inoculated into basic MS medium and into MS medium supplemented with 2.5 μM, 3.5 μM and 4.5 μM of BAP, and kept in a growth room for 40 days under controlled photoperiod and temperature conditions. A 2x4 factorial design was adopted, with three replicates. Analyzed variables included shoot percentage, callogenesis and oxidations, and means were compared by the Tukey test at the 5% probability level. Results showed a signifi cant infl uence of BAP only on shoot induction, and of explant type on that variable and on other variables too. It was concluded that, under in vitro conditions, the nodal type of explant is more responsive to BAP action and that 2.5 μM is the recommended concentration for shoot induction in brazilwood. 
publishDate 2015
dc.date.none.fl_str_mv 2015-05-11
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://cerne.ufla.br/site/index.php/CERNE/article/view/53
url https://cerne.ufla.br/site/index.php/CERNE/article/view/53
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://cerne.ufla.br/site/index.php/CERNE/article/view/53/45
dc.rights.driver.fl_str_mv Copyright (c) 2015 Ana Katarina Oliveira Aragão, Magdi Ahmed Ibrahim Aloufa, Igor do Amaral Costa
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2015 Ana Katarina Oliveira Aragão, Magdi Ahmed Ibrahim Aloufa, Igor do Amaral Costa
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv CERNE
CERNE
publisher.none.fl_str_mv CERNE
CERNE
dc.source.none.fl_str_mv CERNE; Vol. 17 No. 3 (2011); 339-345
CERNE; v. 17 n. 3 (2011); 339-345
2317-6342
0104-7760
reponame:Cerne (Online)
instname:Universidade Federal de Lavras (UFLA)
instacron:UFLA
instname_str Universidade Federal de Lavras (UFLA)
instacron_str UFLA
institution UFLA
reponame_str Cerne (Online)
collection Cerne (Online)
repository.name.fl_str_mv Cerne (Online) - Universidade Federal de Lavras (UFLA)
repository.mail.fl_str_mv cerne@dcf.ufla.br||cerne@dcf.ufla.br
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