EFFECT OF BAP (6-BENZYLAMINOPURINE) ON SHOOT INDUCTION IN EXPLANTS OF BRAZILWOOD
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Cerne (Online) |
Texto Completo: | https://cerne.ufla.br/site/index.php/CERNE/article/view/53 |
Resumo: | The Brazilian Atlantic forest has been subjected to intense degradation, with only about 7% to 8% of its original area remaining today. This situation has raised concerns over the conservation of species threatened with extinction. In all, 276 tree and bush species are under threat, out of which this study chose to evaluate alternatives for protecting brazilwood ‘Pau-Brasil’ (Caesalpinia echinata Lam.). Most studies performed so far on this subject either evaluate the effect of cytokinins on induction of callogenesis or focus on improving cryopreservation methodologies. In an attempt to expand knowledge about biotechnological techniques enabling conservation of C. echinata, this work evaluated the effect of 6-benzylaminopurine (BAP) and explant type on induction of shoots in brazilwood. To attain that, explants were inoculated into basic MS medium and into MS medium supplemented with 2.5 μM, 3.5 μM and 4.5 μM of BAP, and kept in a growth room for 40 days under controlled photoperiod and temperature conditions. A 2x4 factorial design was adopted, with three replicates. Analyzed variables included shoot percentage, callogenesis and oxidations, and means were compared by the Tukey test at the 5% probability level. Results showed a signifi cant infl uence of BAP only on shoot induction, and of explant type on that variable and on other variables too. It was concluded that, under in vitro conditions, the nodal type of explant is more responsive to BAP action and that 2.5 μM is the recommended concentration for shoot induction in brazilwood. |
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EFFECT OF BAP (6-BENZYLAMINOPURINE) ON SHOOT INDUCTION IN EXPLANTS OF BRAZILWOODIn vitro micropropagationcytokinincallogenesis and oxidationsThe Brazilian Atlantic forest has been subjected to intense degradation, with only about 7% to 8% of its original area remaining today. This situation has raised concerns over the conservation of species threatened with extinction. In all, 276 tree and bush species are under threat, out of which this study chose to evaluate alternatives for protecting brazilwood ‘Pau-Brasil’ (Caesalpinia echinata Lam.). Most studies performed so far on this subject either evaluate the effect of cytokinins on induction of callogenesis or focus on improving cryopreservation methodologies. In an attempt to expand knowledge about biotechnological techniques enabling conservation of C. echinata, this work evaluated the effect of 6-benzylaminopurine (BAP) and explant type on induction of shoots in brazilwood. To attain that, explants were inoculated into basic MS medium and into MS medium supplemented with 2.5 μM, 3.5 μM and 4.5 μM of BAP, and kept in a growth room for 40 days under controlled photoperiod and temperature conditions. A 2x4 factorial design was adopted, with three replicates. Analyzed variables included shoot percentage, callogenesis and oxidations, and means were compared by the Tukey test at the 5% probability level. Results showed a signifi cant infl uence of BAP only on shoot induction, and of explant type on that variable and on other variables too. It was concluded that, under in vitro conditions, the nodal type of explant is more responsive to BAP action and that 2.5 μM is the recommended concentration for shoot induction in brazilwood. CERNECERNE2015-05-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://cerne.ufla.br/site/index.php/CERNE/article/view/53CERNE; Vol. 17 No. 3 (2011); 339-345CERNE; v. 17 n. 3 (2011); 339-3452317-63420104-7760reponame:Cerne (Online)instname:Universidade Federal de Lavras (UFLA)instacron:UFLAporhttps://cerne.ufla.br/site/index.php/CERNE/article/view/53/45Copyright (c) 2015 Ana Katarina Oliveira Aragão, Magdi Ahmed Ibrahim Aloufa, Igor do Amaral Costainfo:eu-repo/semantics/openAccessAragão, Ana Katarina OliveiraAloufa, Magdi Ahmed IbrahimCosta, Igor do Amaral2015-05-12T11:04:19Zoai:cerne.ufla.br:article/53Revistahttps://cerne.ufla.br/site/index.php/CERNEPUBhttps://cerne.ufla.br/site/index.php/CERNE/oaicerne@dcf.ufla.br||cerne@dcf.ufla.br2317-63420104-7760opendoar:2024-05-21T19:53:28.196132Cerne (Online) - Universidade Federal de Lavras (UFLA)true |
dc.title.none.fl_str_mv |
EFFECT OF BAP (6-BENZYLAMINOPURINE) ON SHOOT INDUCTION IN EXPLANTS OF BRAZILWOOD |
title |
EFFECT OF BAP (6-BENZYLAMINOPURINE) ON SHOOT INDUCTION IN EXPLANTS OF BRAZILWOOD |
spellingShingle |
EFFECT OF BAP (6-BENZYLAMINOPURINE) ON SHOOT INDUCTION IN EXPLANTS OF BRAZILWOOD Aragão, Ana Katarina Oliveira In vitro micropropagation cytokinin callogenesis and oxidations |
title_short |
EFFECT OF BAP (6-BENZYLAMINOPURINE) ON SHOOT INDUCTION IN EXPLANTS OF BRAZILWOOD |
title_full |
EFFECT OF BAP (6-BENZYLAMINOPURINE) ON SHOOT INDUCTION IN EXPLANTS OF BRAZILWOOD |
title_fullStr |
EFFECT OF BAP (6-BENZYLAMINOPURINE) ON SHOOT INDUCTION IN EXPLANTS OF BRAZILWOOD |
title_full_unstemmed |
EFFECT OF BAP (6-BENZYLAMINOPURINE) ON SHOOT INDUCTION IN EXPLANTS OF BRAZILWOOD |
title_sort |
EFFECT OF BAP (6-BENZYLAMINOPURINE) ON SHOOT INDUCTION IN EXPLANTS OF BRAZILWOOD |
author |
Aragão, Ana Katarina Oliveira |
author_facet |
Aragão, Ana Katarina Oliveira Aloufa, Magdi Ahmed Ibrahim Costa, Igor do Amaral |
author_role |
author |
author2 |
Aloufa, Magdi Ahmed Ibrahim Costa, Igor do Amaral |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Aragão, Ana Katarina Oliveira Aloufa, Magdi Ahmed Ibrahim Costa, Igor do Amaral |
dc.subject.por.fl_str_mv |
In vitro micropropagation cytokinin callogenesis and oxidations |
topic |
In vitro micropropagation cytokinin callogenesis and oxidations |
description |
The Brazilian Atlantic forest has been subjected to intense degradation, with only about 7% to 8% of its original area remaining today. This situation has raised concerns over the conservation of species threatened with extinction. In all, 276 tree and bush species are under threat, out of which this study chose to evaluate alternatives for protecting brazilwood ‘Pau-Brasil’ (Caesalpinia echinata Lam.). Most studies performed so far on this subject either evaluate the effect of cytokinins on induction of callogenesis or focus on improving cryopreservation methodologies. In an attempt to expand knowledge about biotechnological techniques enabling conservation of C. echinata, this work evaluated the effect of 6-benzylaminopurine (BAP) and explant type on induction of shoots in brazilwood. To attain that, explants were inoculated into basic MS medium and into MS medium supplemented with 2.5 μM, 3.5 μM and 4.5 μM of BAP, and kept in a growth room for 40 days under controlled photoperiod and temperature conditions. A 2x4 factorial design was adopted, with three replicates. Analyzed variables included shoot percentage, callogenesis and oxidations, and means were compared by the Tukey test at the 5% probability level. Results showed a signifi cant infl uence of BAP only on shoot induction, and of explant type on that variable and on other variables too. It was concluded that, under in vitro conditions, the nodal type of explant is more responsive to BAP action and that 2.5 μM is the recommended concentration for shoot induction in brazilwood. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-05-11 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://cerne.ufla.br/site/index.php/CERNE/article/view/53 |
url |
https://cerne.ufla.br/site/index.php/CERNE/article/view/53 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://cerne.ufla.br/site/index.php/CERNE/article/view/53/45 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2015 Ana Katarina Oliveira Aragão, Magdi Ahmed Ibrahim Aloufa, Igor do Amaral Costa info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2015 Ana Katarina Oliveira Aragão, Magdi Ahmed Ibrahim Aloufa, Igor do Amaral Costa |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
CERNE CERNE |
publisher.none.fl_str_mv |
CERNE CERNE |
dc.source.none.fl_str_mv |
CERNE; Vol. 17 No. 3 (2011); 339-345 CERNE; v. 17 n. 3 (2011); 339-345 2317-6342 0104-7760 reponame:Cerne (Online) instname:Universidade Federal de Lavras (UFLA) instacron:UFLA |
instname_str |
Universidade Federal de Lavras (UFLA) |
instacron_str |
UFLA |
institution |
UFLA |
reponame_str |
Cerne (Online) |
collection |
Cerne (Online) |
repository.name.fl_str_mv |
Cerne (Online) - Universidade Federal de Lavras (UFLA) |
repository.mail.fl_str_mv |
cerne@dcf.ufla.br||cerne@dcf.ufla.br |
_version_ |
1799874939133624320 |