Evaluation of methylene blue mediated photodynamic therapy in wound healing: in vivo experimental study

• Autor(a) principal: Neri, Erick Souza
• Data de Publicação: 2021
• Outros Autores: Nascimento Júnior, Charton Frankson Madureira, Lima, Taynara Camille Guilherme, Moreira, Rafael Pires, Barcessat, Ana Rita Pinheiro
• Tipo de documento: Artigo
• Idioma: por
• Título da fonte: Revista Enfermagem Atual In Derme
• Texto Completo: https://revistaenfermagematual.com/index.php/revista/article/view/1247
• topic: Fototerapia; Fotoquimioterapia; Azul de metileno; Cicatrização; Ferimentos e lesões.; Phototherapy; Photochemotherapy; Methylene blue; Wound healing; Wounds and injuries.

Objective: To evaluate the tissue effects of a single application of Photodynamic Therapy on lesions induced in experimental models, determining their influence on collagen formation and cell cycle duration. Method: Fifty lesions induced in Mus Musculus mice were randomly treated with 0.01% Methylene Blue mediated Photodynamic Therapy activated by 660 nm Red Laser (G1), Lasertherapy without photosensitizer (G2), 0.01% Methylene Blue (G3), Dermatological Ointment as positive control (G4) and Distilled Water as negative control (G5), being histologically evaluated for collagen deposition and areas of nucleolar organization after 72 hours of treatment. Results: The photoactivated groups showed a significant difference regarding collagen deposition (p<0,05) by the Massons Trichrome Stain, showing evidence of reduced repair time. There was no significant difference (p>0.05) by the AgNOR technique in the nucleolar organizer regions. Conclusions: Photodynamic Therapy and Laser Photobiomodulation showed evidence of collagen deposition in the first hours of the healing process, without necessarily changing the duration of the local cell cycle, not exerting acceleration in the cell proliferation process under the experimental conditions evaluated in this study.