Somatic embryogenesis in cassava genotypes from the northeast of Brazil

Detalhes bibliográficos
Autor(a) principal: Feitosa,Terezinha
Data de Publicação: 2007
Outros Autores: Bastos,João Luíz Pinheiro, Ponte,Luíz Ferreira Aguiar, Jucá,Thiago Lustosa, Campos,Francisco de Assis de Paiva
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Archives of Biology and Technology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000200004
Resumo: A method for the induction of somatic embryogenesis in eight cassava genotypes from northeastern Brazil is described. The explants used were shoot apexes isolated both from in vitro grown plants and from shoots that sprouted from stem cuttings. Somatic embryogenesis was achieved in high frequencies by the addition in the induction medium of the auxin picloram over a wide range of concentrations. Green cotyledons of primary somatic embryos were used as explants to induce somatic (cyclic) secondary embryogenesis in an inducing medium supplemented with picloram at 12 mg/L. The method could be used not only for the mass production of plants of the cassava genotypes, but also to generate explants (green cotyledons of somatic embryos) as themselves excellent targets for genetic transformation.
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spelling Somatic embryogenesis in cassava genotypes from the northeast of BrazilManihot esculentacassavasomatic embryogenesisplant tissue culturepicloramA method for the induction of somatic embryogenesis in eight cassava genotypes from northeastern Brazil is described. The explants used were shoot apexes isolated both from in vitro grown plants and from shoots that sprouted from stem cuttings. Somatic embryogenesis was achieved in high frequencies by the addition in the induction medium of the auxin picloram over a wide range of concentrations. Green cotyledons of primary somatic embryos were used as explants to induce somatic (cyclic) secondary embryogenesis in an inducing medium supplemented with picloram at 12 mg/L. The method could be used not only for the mass production of plants of the cassava genotypes, but also to generate explants (green cotyledons of somatic embryos) as themselves excellent targets for genetic transformation.Instituto de Tecnologia do Paraná - Tecpar2007-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000200004Brazilian Archives of Biology and Technology v.50 n.2 2007reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/S1516-89132007000200004info:eu-repo/semantics/openAccessFeitosa,TerezinhaBastos,João Luíz PinheiroPonte,Luíz Ferreira AguiarJucá,Thiago LustosaCampos,Francisco de Assis de Paivaeng2007-06-12T00:00:00Zoai:scielo:S1516-89132007000200004Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2007-06-12T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false
dc.title.none.fl_str_mv Somatic embryogenesis in cassava genotypes from the northeast of Brazil
title Somatic embryogenesis in cassava genotypes from the northeast of Brazil
spellingShingle Somatic embryogenesis in cassava genotypes from the northeast of Brazil
Feitosa,Terezinha
Manihot esculenta
cassava
somatic embryogenesis
plant tissue culture
picloram
title_short Somatic embryogenesis in cassava genotypes from the northeast of Brazil
title_full Somatic embryogenesis in cassava genotypes from the northeast of Brazil
title_fullStr Somatic embryogenesis in cassava genotypes from the northeast of Brazil
title_full_unstemmed Somatic embryogenesis in cassava genotypes from the northeast of Brazil
title_sort Somatic embryogenesis in cassava genotypes from the northeast of Brazil
author Feitosa,Terezinha
author_facet Feitosa,Terezinha
Bastos,João Luíz Pinheiro
Ponte,Luíz Ferreira Aguiar
Jucá,Thiago Lustosa
Campos,Francisco de Assis de Paiva
author_role author
author2 Bastos,João Luíz Pinheiro
Ponte,Luíz Ferreira Aguiar
Jucá,Thiago Lustosa
Campos,Francisco de Assis de Paiva
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Feitosa,Terezinha
Bastos,João Luíz Pinheiro
Ponte,Luíz Ferreira Aguiar
Jucá,Thiago Lustosa
Campos,Francisco de Assis de Paiva
dc.subject.por.fl_str_mv Manihot esculenta
cassava
somatic embryogenesis
plant tissue culture
picloram
topic Manihot esculenta
cassava
somatic embryogenesis
plant tissue culture
picloram
description A method for the induction of somatic embryogenesis in eight cassava genotypes from northeastern Brazil is described. The explants used were shoot apexes isolated both from in vitro grown plants and from shoots that sprouted from stem cuttings. Somatic embryogenesis was achieved in high frequencies by the addition in the induction medium of the auxin picloram over a wide range of concentrations. Green cotyledons of primary somatic embryos were used as explants to induce somatic (cyclic) secondary embryogenesis in an inducing medium supplemented with picloram at 12 mg/L. The method could be used not only for the mass production of plants of the cassava genotypes, but also to generate explants (green cotyledons of somatic embryos) as themselves excellent targets for genetic transformation.
publishDate 2007
dc.date.none.fl_str_mv 2007-03-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000200004
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000200004
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1516-89132007000200004
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
dc.source.none.fl_str_mv Brazilian Archives of Biology and Technology v.50 n.2 2007
reponame:Brazilian Archives of Biology and Technology
instname:Instituto de Tecnologia do Paraná (Tecpar)
instacron:TECPAR
instname_str Instituto de Tecnologia do Paraná (Tecpar)
instacron_str TECPAR
institution TECPAR
reponame_str Brazilian Archives of Biology and Technology
collection Brazilian Archives of Biology and Technology
repository.name.fl_str_mv Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)
repository.mail.fl_str_mv babt@tecpar.br||babt@tecpar.br
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