Characterization of Nomuraea rileyi strains using polymorphic DNA, virulence and enzyme activity

Detalhes bibliográficos
Autor(a) principal: Vargas,Lúcia Rosane Bertholdo
Data de Publicação: 2003
Outros Autores: Rossato,Marcelo, Ribeiro,Rute Terezinha da Silva, Barros,Neiva Monteiro de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Archives of Biology and Technology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000100003
Resumo: The characterization of entomopathogenic microorganisms is important for the selection of more effective strains for use in integrated pest-control programs. Five Nomuraea rileyi strains (SA86101, GU87401, SR86151, CG128 and VA9101) were characterized using random amplified polymorphic DNA (RAPD) analysis, virulence studies and assessment of chitinolytic and proteolytic activity. RAPD analysis divided the strains into two groups with a similarity coefficient of 0,76%, group 1 consisting of strains SA86101, GU87401 and SR86151 and group 2 of strains CG128 and VA9101. The LT50 varied from 165h with strain VA9101 to 246h with strain GU87401. Chitinolytic and proteolytic activity of the fungi after 144h growth in minimal medium were tested using colloidal chitin as substrate. All strains exhibited enzyme activity, with strain VA9101 having the highest chitinase activity (0,0040 mumol/mL/min the 40ºC) and strain SA86101 the highest proteolytic activity. No relationship was found between RAPD analysis, virulence and chitinase or protease activity.
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spelling Characterization of Nomuraea rileyi strains using polymorphic DNA, virulence and enzyme activityNomuraea rileyiAnticarsia gemmatalischitinaseproteaseRAPDvirulenceThe characterization of entomopathogenic microorganisms is important for the selection of more effective strains for use in integrated pest-control programs. Five Nomuraea rileyi strains (SA86101, GU87401, SR86151, CG128 and VA9101) were characterized using random amplified polymorphic DNA (RAPD) analysis, virulence studies and assessment of chitinolytic and proteolytic activity. RAPD analysis divided the strains into two groups with a similarity coefficient of 0,76%, group 1 consisting of strains SA86101, GU87401 and SR86151 and group 2 of strains CG128 and VA9101. The LT50 varied from 165h with strain VA9101 to 246h with strain GU87401. Chitinolytic and proteolytic activity of the fungi after 144h growth in minimal medium were tested using colloidal chitin as substrate. All strains exhibited enzyme activity, with strain VA9101 having the highest chitinase activity (0,0040 mumol/mL/min the 40ºC) and strain SA86101 the highest proteolytic activity. No relationship was found between RAPD analysis, virulence and chitinase or protease activity.Instituto de Tecnologia do Paraná - Tecpar2003-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000100003Brazilian Archives of Biology and Technology v.46 n.1 2003reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/S1516-89132003000100003info:eu-repo/semantics/openAccessVargas,Lúcia Rosane BertholdoRossato,MarceloRibeiro,Rute Terezinha da SilvaBarros,Neiva Monteiro deeng2003-05-19T00:00:00Zoai:scielo:S1516-89132003000100003Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2003-05-19T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false
dc.title.none.fl_str_mv Characterization of Nomuraea rileyi strains using polymorphic DNA, virulence and enzyme activity
title Characterization of Nomuraea rileyi strains using polymorphic DNA, virulence and enzyme activity
spellingShingle Characterization of Nomuraea rileyi strains using polymorphic DNA, virulence and enzyme activity
Vargas,Lúcia Rosane Bertholdo
Nomuraea rileyi
Anticarsia gemmatalis
chitinase
protease
RAPD
virulence
title_short Characterization of Nomuraea rileyi strains using polymorphic DNA, virulence and enzyme activity
title_full Characterization of Nomuraea rileyi strains using polymorphic DNA, virulence and enzyme activity
title_fullStr Characterization of Nomuraea rileyi strains using polymorphic DNA, virulence and enzyme activity
title_full_unstemmed Characterization of Nomuraea rileyi strains using polymorphic DNA, virulence and enzyme activity
title_sort Characterization of Nomuraea rileyi strains using polymorphic DNA, virulence and enzyme activity
author Vargas,Lúcia Rosane Bertholdo
author_facet Vargas,Lúcia Rosane Bertholdo
Rossato,Marcelo
Ribeiro,Rute Terezinha da Silva
Barros,Neiva Monteiro de
author_role author
author2 Rossato,Marcelo
Ribeiro,Rute Terezinha da Silva
Barros,Neiva Monteiro de
author2_role author
author
author
dc.contributor.author.fl_str_mv Vargas,Lúcia Rosane Bertholdo
Rossato,Marcelo
Ribeiro,Rute Terezinha da Silva
Barros,Neiva Monteiro de
dc.subject.por.fl_str_mv Nomuraea rileyi
Anticarsia gemmatalis
chitinase
protease
RAPD
virulence
topic Nomuraea rileyi
Anticarsia gemmatalis
chitinase
protease
RAPD
virulence
description The characterization of entomopathogenic microorganisms is important for the selection of more effective strains for use in integrated pest-control programs. Five Nomuraea rileyi strains (SA86101, GU87401, SR86151, CG128 and VA9101) were characterized using random amplified polymorphic DNA (RAPD) analysis, virulence studies and assessment of chitinolytic and proteolytic activity. RAPD analysis divided the strains into two groups with a similarity coefficient of 0,76%, group 1 consisting of strains SA86101, GU87401 and SR86151 and group 2 of strains CG128 and VA9101. The LT50 varied from 165h with strain VA9101 to 246h with strain GU87401. Chitinolytic and proteolytic activity of the fungi after 144h growth in minimal medium were tested using colloidal chitin as substrate. All strains exhibited enzyme activity, with strain VA9101 having the highest chitinase activity (0,0040 mumol/mL/min the 40ºC) and strain SA86101 the highest proteolytic activity. No relationship was found between RAPD analysis, virulence and chitinase or protease activity.
publishDate 2003
dc.date.none.fl_str_mv 2003-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
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dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000100003
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000100003
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1516-89132003000100003
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
dc.source.none.fl_str_mv Brazilian Archives of Biology and Technology v.46 n.1 2003
reponame:Brazilian Archives of Biology and Technology
instname:Instituto de Tecnologia do Paraná (Tecpar)
instacron:TECPAR
instname_str Instituto de Tecnologia do Paraná (Tecpar)
instacron_str TECPAR
institution TECPAR
reponame_str Brazilian Archives of Biology and Technology
collection Brazilian Archives of Biology and Technology
repository.name.fl_str_mv Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)
repository.mail.fl_str_mv babt@tecpar.br||babt@tecpar.br
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