LC50 of the Peptide Produced by the Entomopathogenic Fungus Nomuraea rileyi (Farlow) Samson Active Against Third Instar Larvae of Anticarsia gemmatalis (Lep.: Noctuidae)

Detalhes bibliográficos
Autor(a) principal: Onofre,Sideney Becker
Data de Publicação: 2002
Outros Autores: Riveros Gonzalez,Raul, Messias,Cláudio Luiz, Azevedo,João Lúcio, Barros,Neiva Monteiro de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Archives of Biology and Technology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132002000300004
Resumo: The entomopathogenic fungus Nomuraea rileyi (Farlow) Samson produced a peptide active against Anticarsia gemmatalis 3rd instar larvae. To produce this peptide, N. rileyi was cultivated aerobically in Saboraud, maltose, yeast-extract broth at 26 ± 1ºC for 12 days, after which the medium was filtered and separated in a liquid/liquid extractor, concentrated and the peptide purified chromatographically. The crystals obtained were kept refrigerated until needed for LC50 analysis. The LC50 of this peptide against A. gemmatalis 3rd instar larvae was determined in triplicate experiments using solutions containing 1.0, 0.2, 0.1, 0.01, 0.001 and 0.0001 mg/ml of N. rileyi peptide. The results of these experiments were used to calculate a linear equation in which Y = 6,81176 + 1,01382 * LOGx , giving a LC50 value of 0.0163 mg/ml.
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spelling LC50 of the Peptide Produced by the Entomopathogenic Fungus Nomuraea rileyi (Farlow) Samson Active Against Third Instar Larvae of Anticarsia gemmatalis (Lep.: Noctuidae)Anticarsia gemmatalisNomuraea rileyiintegrated controlsecondary metabolitetoxinsThe entomopathogenic fungus Nomuraea rileyi (Farlow) Samson produced a peptide active against Anticarsia gemmatalis 3rd instar larvae. To produce this peptide, N. rileyi was cultivated aerobically in Saboraud, maltose, yeast-extract broth at 26 ± 1ºC for 12 days, after which the medium was filtered and separated in a liquid/liquid extractor, concentrated and the peptide purified chromatographically. The crystals obtained were kept refrigerated until needed for LC50 analysis. The LC50 of this peptide against A. gemmatalis 3rd instar larvae was determined in triplicate experiments using solutions containing 1.0, 0.2, 0.1, 0.01, 0.001 and 0.0001 mg/ml of N. rileyi peptide. The results of these experiments were used to calculate a linear equation in which Y = 6,81176 + 1,01382 * LOGx , giving a LC50 value of 0.0163 mg/ml.Instituto de Tecnologia do Paraná - Tecpar2002-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132002000300004Brazilian Archives of Biology and Technology v.45 n.3 2002reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/S1516-89132002000300004info:eu-repo/semantics/openAccessOnofre,Sideney BeckerRiveros Gonzalez,RaulMessias,Cláudio LuizAzevedo,João LúcioBarros,Neiva Monteiro deeng2002-10-23T00:00:00Zoai:scielo:S1516-89132002000300004Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2002-10-23T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false
dc.title.none.fl_str_mv LC50 of the Peptide Produced by the Entomopathogenic Fungus Nomuraea rileyi (Farlow) Samson Active Against Third Instar Larvae of Anticarsia gemmatalis (Lep.: Noctuidae)
title LC50 of the Peptide Produced by the Entomopathogenic Fungus Nomuraea rileyi (Farlow) Samson Active Against Third Instar Larvae of Anticarsia gemmatalis (Lep.: Noctuidae)
spellingShingle LC50 of the Peptide Produced by the Entomopathogenic Fungus Nomuraea rileyi (Farlow) Samson Active Against Third Instar Larvae of Anticarsia gemmatalis (Lep.: Noctuidae)
Onofre,Sideney Becker
Anticarsia gemmatalis
Nomuraea rileyi
integrated control
secondary metabolite
toxins
title_short LC50 of the Peptide Produced by the Entomopathogenic Fungus Nomuraea rileyi (Farlow) Samson Active Against Third Instar Larvae of Anticarsia gemmatalis (Lep.: Noctuidae)
title_full LC50 of the Peptide Produced by the Entomopathogenic Fungus Nomuraea rileyi (Farlow) Samson Active Against Third Instar Larvae of Anticarsia gemmatalis (Lep.: Noctuidae)
title_fullStr LC50 of the Peptide Produced by the Entomopathogenic Fungus Nomuraea rileyi (Farlow) Samson Active Against Third Instar Larvae of Anticarsia gemmatalis (Lep.: Noctuidae)
title_full_unstemmed LC50 of the Peptide Produced by the Entomopathogenic Fungus Nomuraea rileyi (Farlow) Samson Active Against Third Instar Larvae of Anticarsia gemmatalis (Lep.: Noctuidae)
title_sort LC50 of the Peptide Produced by the Entomopathogenic Fungus Nomuraea rileyi (Farlow) Samson Active Against Third Instar Larvae of Anticarsia gemmatalis (Lep.: Noctuidae)
author Onofre,Sideney Becker
author_facet Onofre,Sideney Becker
Riveros Gonzalez,Raul
Messias,Cláudio Luiz
Azevedo,João Lúcio
Barros,Neiva Monteiro de
author_role author
author2 Riveros Gonzalez,Raul
Messias,Cláudio Luiz
Azevedo,João Lúcio
Barros,Neiva Monteiro de
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Onofre,Sideney Becker
Riveros Gonzalez,Raul
Messias,Cláudio Luiz
Azevedo,João Lúcio
Barros,Neiva Monteiro de
dc.subject.por.fl_str_mv Anticarsia gemmatalis
Nomuraea rileyi
integrated control
secondary metabolite
toxins
topic Anticarsia gemmatalis
Nomuraea rileyi
integrated control
secondary metabolite
toxins
description The entomopathogenic fungus Nomuraea rileyi (Farlow) Samson produced a peptide active against Anticarsia gemmatalis 3rd instar larvae. To produce this peptide, N. rileyi was cultivated aerobically in Saboraud, maltose, yeast-extract broth at 26 ± 1ºC for 12 days, after which the medium was filtered and separated in a liquid/liquid extractor, concentrated and the peptide purified chromatographically. The crystals obtained were kept refrigerated until needed for LC50 analysis. The LC50 of this peptide against A. gemmatalis 3rd instar larvae was determined in triplicate experiments using solutions containing 1.0, 0.2, 0.1, 0.01, 0.001 and 0.0001 mg/ml of N. rileyi peptide. The results of these experiments were used to calculate a linear equation in which Y = 6,81176 + 1,01382 * LOGx , giving a LC50 value of 0.0163 mg/ml.
publishDate 2002
dc.date.none.fl_str_mv 2002-09-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132002000300004
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132002000300004
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1516-89132002000300004
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
dc.source.none.fl_str_mv Brazilian Archives of Biology and Technology v.45 n.3 2002
reponame:Brazilian Archives of Biology and Technology
instname:Instituto de Tecnologia do Paraná (Tecpar)
instacron:TECPAR
instname_str Instituto de Tecnologia do Paraná (Tecpar)
instacron_str TECPAR
institution TECPAR
reponame_str Brazilian Archives of Biology and Technology
collection Brazilian Archives of Biology and Technology
repository.name.fl_str_mv Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)
repository.mail.fl_str_mv babt@tecpar.br||babt@tecpar.br
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