Aspergillus niveus Blochwitz 4128URM: new source for inulinase production

• Autor(a) principal: Souza-Motta,Cristina Maria de
• Data de Publicação: 2005
• Outros Autores: Cavalcanti,Maria Auxiliadora de Queiroz, Porto,Ana Lúcia Figueiredo, Moreira,Keila Aparecida, Lima Filho,José Luiz de
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Brazilian Archives of Biology and Technology
• Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000300003
• topic: Aspergillus niveus; rhizosphere; inulinase; inulin; purification

Aspergillus niveus Blochwitz 4128 URM isolated from sunflower rhizosphere demonstrated a new source of inulinase. The enzyme was produced in culture medium containing inulin as substrate in the concentrations: 10, 15 and 20g L-1. Maximum enzyme activity was obtained in medium containing 20g L-1 inulin. The enzyme was partially purified using ammonium sulphate precipitation, followed by ion charge (DE-32) and molecular exclusion (Sephadex) chromatography. The results showed the optimal pH and temperature of inulinase from crude extract were 4.0 and 4.8 and 45ºC, respectively. The enzyme was purified 34.65 fold with yield of 53.63%. A. niveus 4128URM can be used in the inulinase production with use in the food industries.