Tc-99m direct radiolabeling of monoclonal antibody ior egf/r3: quality control and image studies in mice
Autor(a) principal: | |
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Data de Publicação: | 2005 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Archives of Biology and Technology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000700004 |
Resumo: | Monoclonal antibodies (Mabs) have been useful for immunoscintigraphic applications in clinical diagnosis since they were introduced in the practice of nuclear medicine. The ior egf/r3 (Centis, Cuba) is a murine monoclonal antibody against epidermal growth factor receptor (EGF-R) and has been widely used in the radioimmunodiagnosis of tumors of epithelial origin. Labeled with 99mTc, its main application in Nuclear Medicine is the follow up, detection and evaluation of tumor recurrences. The objective of this work is to describe the preparation of a lyophilized formulation (kit) for radiolabeling the Mab ior egf/r3 with 99mTc for immunoscintigraphic applications. Radiolabeling efficiency, effects on immunoreactivity, image studies and stability of the formulation are reported. The study demonstrated that the kit formulation can be labeled with 99mTc at high yields and can be used to visualize in vivo human tumors of epithelial origin by immunoscintigraphy studies. |
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Tc-99m direct radiolabeling of monoclonal antibody ior egf/r3: quality control and image studies in miceRadiolabelingtechnetiummonoclonal antibodiesnuclear medicineMonoclonal antibodies (Mabs) have been useful for immunoscintigraphic applications in clinical diagnosis since they were introduced in the practice of nuclear medicine. The ior egf/r3 (Centis, Cuba) is a murine monoclonal antibody against epidermal growth factor receptor (EGF-R) and has been widely used in the radioimmunodiagnosis of tumors of epithelial origin. Labeled with 99mTc, its main application in Nuclear Medicine is the follow up, detection and evaluation of tumor recurrences. The objective of this work is to describe the preparation of a lyophilized formulation (kit) for radiolabeling the Mab ior egf/r3 with 99mTc for immunoscintigraphic applications. Radiolabeling efficiency, effects on immunoreactivity, image studies and stability of the formulation are reported. The study demonstrated that the kit formulation can be labeled with 99mTc at high yields and can be used to visualize in vivo human tumors of epithelial origin by immunoscintigraphy studies.Instituto de Tecnologia do Paraná - Tecpar2005-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000700004Brazilian Archives of Biology and Technology v.48 n.spe2 2005reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/S1516-89132005000700004info:eu-repo/semantics/openAccessDias,Carla RobertaMarczewski,BarbaraMoraes,VanessaBarboza,Marycel Figols deOsso Junior,João Albertoeng2005-12-09T00:00:00Zoai:scielo:S1516-89132005000700004Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2005-12-09T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false |
dc.title.none.fl_str_mv |
Tc-99m direct radiolabeling of monoclonal antibody ior egf/r3: quality control and image studies in mice |
title |
Tc-99m direct radiolabeling of monoclonal antibody ior egf/r3: quality control and image studies in mice |
spellingShingle |
Tc-99m direct radiolabeling of monoclonal antibody ior egf/r3: quality control and image studies in mice Dias,Carla Roberta Radiolabeling technetium monoclonal antibodies nuclear medicine |
title_short |
Tc-99m direct radiolabeling of monoclonal antibody ior egf/r3: quality control and image studies in mice |
title_full |
Tc-99m direct radiolabeling of monoclonal antibody ior egf/r3: quality control and image studies in mice |
title_fullStr |
Tc-99m direct radiolabeling of monoclonal antibody ior egf/r3: quality control and image studies in mice |
title_full_unstemmed |
Tc-99m direct radiolabeling of monoclonal antibody ior egf/r3: quality control and image studies in mice |
title_sort |
Tc-99m direct radiolabeling of monoclonal antibody ior egf/r3: quality control and image studies in mice |
author |
Dias,Carla Roberta |
author_facet |
Dias,Carla Roberta Marczewski,Barbara Moraes,Vanessa Barboza,Marycel Figols de Osso Junior,João Alberto |
author_role |
author |
author2 |
Marczewski,Barbara Moraes,Vanessa Barboza,Marycel Figols de Osso Junior,João Alberto |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Dias,Carla Roberta Marczewski,Barbara Moraes,Vanessa Barboza,Marycel Figols de Osso Junior,João Alberto |
dc.subject.por.fl_str_mv |
Radiolabeling technetium monoclonal antibodies nuclear medicine |
topic |
Radiolabeling technetium monoclonal antibodies nuclear medicine |
description |
Monoclonal antibodies (Mabs) have been useful for immunoscintigraphic applications in clinical diagnosis since they were introduced in the practice of nuclear medicine. The ior egf/r3 (Centis, Cuba) is a murine monoclonal antibody against epidermal growth factor receptor (EGF-R) and has been widely used in the radioimmunodiagnosis of tumors of epithelial origin. Labeled with 99mTc, its main application in Nuclear Medicine is the follow up, detection and evaluation of tumor recurrences. The objective of this work is to describe the preparation of a lyophilized formulation (kit) for radiolabeling the Mab ior egf/r3 with 99mTc for immunoscintigraphic applications. Radiolabeling efficiency, effects on immunoreactivity, image studies and stability of the formulation are reported. The study demonstrated that the kit formulation can be labeled with 99mTc at high yields and can be used to visualize in vivo human tumors of epithelial origin by immunoscintigraphy studies. |
publishDate |
2005 |
dc.date.none.fl_str_mv |
2005-10-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000700004 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000700004 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1516-89132005000700004 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Instituto de Tecnologia do Paraná - Tecpar |
publisher.none.fl_str_mv |
Instituto de Tecnologia do Paraná - Tecpar |
dc.source.none.fl_str_mv |
Brazilian Archives of Biology and Technology v.48 n.spe2 2005 reponame:Brazilian Archives of Biology and Technology instname:Instituto de Tecnologia do Paraná (Tecpar) instacron:TECPAR |
instname_str |
Instituto de Tecnologia do Paraná (Tecpar) |
instacron_str |
TECPAR |
institution |
TECPAR |
reponame_str |
Brazilian Archives of Biology and Technology |
collection |
Brazilian Archives of Biology and Technology |
repository.name.fl_str_mv |
Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar) |
repository.mail.fl_str_mv |
babt@tecpar.br||babt@tecpar.br |
_version_ |
1750318270656806912 |