Phenotypic and molecular characterization of Staphylococcus xylosus: technological potential for use in fermented sausage
Autor(a) principal: | |
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Data de Publicação: | 2009 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Archives of Biology and Technology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000300026 |
Resumo: | Micrococcaceae strains are applied to fermented sausage as starter cultures, where several members of this family are naturally found. The aim of the present work was to isolate and characterize Staphylococcus xylosus from artisanal sausages produced in South Region of Brazil. From 89 isolates presenting catalase positive and coagulase negative activities, 25 strains were selected for phenotypic characterization. Nine strains identified as Staphylococcus xylosus by API-STAPH were evaluated for their nitrate reduction capacity, which showed satisfactory growth of the strains in the presence of nitrite and sodium chloride, demonstrating their potential for use as starter cultures in fermented sausage. The strains were also evaluated through genus and species-specific PCR, which showed only two as S. xylosus, differing from results found in phenotypic characterization. |
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Brazilian Archives of Biology and Technology |
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Phenotypic and molecular characterization of Staphylococcus xylosus: technological potential for use in fermented sausageStaphylococcus xylosusermented sausagestarter culturesPCRMicrococcaceae strains are applied to fermented sausage as starter cultures, where several members of this family are naturally found. The aim of the present work was to isolate and characterize Staphylococcus xylosus from artisanal sausages produced in South Region of Brazil. From 89 isolates presenting catalase positive and coagulase negative activities, 25 strains were selected for phenotypic characterization. Nine strains identified as Staphylococcus xylosus by API-STAPH were evaluated for their nitrate reduction capacity, which showed satisfactory growth of the strains in the presence of nitrite and sodium chloride, demonstrating their potential for use as starter cultures in fermented sausage. The strains were also evaluated through genus and species-specific PCR, which showed only two as S. xylosus, differing from results found in phenotypic characterization.Instituto de Tecnologia do Paraná - Tecpar2009-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000300026Brazilian Archives of Biology and Technology v.52 n.3 2009reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/S1516-89132009000300026info:eu-repo/semantics/openAccessFiorentini,Ângela MariaSawitzki,Maristela CortezBertol,Teresinha MarisaBrod,Fábio Cristiano AngonesiPelisser,Márcia ReginaArisi,Ana Carolina MaisonnaveSant'Anna,Ernani Sebastiãoeng2009-07-22T00:00:00Zoai:scielo:S1516-89132009000300026Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2009-07-22T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false |
dc.title.none.fl_str_mv |
Phenotypic and molecular characterization of Staphylococcus xylosus: technological potential for use in fermented sausage |
title |
Phenotypic and molecular characterization of Staphylococcus xylosus: technological potential for use in fermented sausage |
spellingShingle |
Phenotypic and molecular characterization of Staphylococcus xylosus: technological potential for use in fermented sausage Fiorentini,Ângela Maria Staphylococcus xylosus ermented sausage starter cultures PCR |
title_short |
Phenotypic and molecular characterization of Staphylococcus xylosus: technological potential for use in fermented sausage |
title_full |
Phenotypic and molecular characterization of Staphylococcus xylosus: technological potential for use in fermented sausage |
title_fullStr |
Phenotypic and molecular characterization of Staphylococcus xylosus: technological potential for use in fermented sausage |
title_full_unstemmed |
Phenotypic and molecular characterization of Staphylococcus xylosus: technological potential for use in fermented sausage |
title_sort |
Phenotypic and molecular characterization of Staphylococcus xylosus: technological potential for use in fermented sausage |
author |
Fiorentini,Ângela Maria |
author_facet |
Fiorentini,Ângela Maria Sawitzki,Maristela Cortez Bertol,Teresinha Marisa Brod,Fábio Cristiano Angonesi Pelisser,Márcia Regina Arisi,Ana Carolina Maisonnave Sant'Anna,Ernani Sebastião |
author_role |
author |
author2 |
Sawitzki,Maristela Cortez Bertol,Teresinha Marisa Brod,Fábio Cristiano Angonesi Pelisser,Márcia Regina Arisi,Ana Carolina Maisonnave Sant'Anna,Ernani Sebastião |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Fiorentini,Ângela Maria Sawitzki,Maristela Cortez Bertol,Teresinha Marisa Brod,Fábio Cristiano Angonesi Pelisser,Márcia Regina Arisi,Ana Carolina Maisonnave Sant'Anna,Ernani Sebastião |
dc.subject.por.fl_str_mv |
Staphylococcus xylosus ermented sausage starter cultures PCR |
topic |
Staphylococcus xylosus ermented sausage starter cultures PCR |
description |
Micrococcaceae strains are applied to fermented sausage as starter cultures, where several members of this family are naturally found. The aim of the present work was to isolate and characterize Staphylococcus xylosus from artisanal sausages produced in South Region of Brazil. From 89 isolates presenting catalase positive and coagulase negative activities, 25 strains were selected for phenotypic characterization. Nine strains identified as Staphylococcus xylosus by API-STAPH were evaluated for their nitrate reduction capacity, which showed satisfactory growth of the strains in the presence of nitrite and sodium chloride, demonstrating their potential for use as starter cultures in fermented sausage. The strains were also evaluated through genus and species-specific PCR, which showed only two as S. xylosus, differing from results found in phenotypic characterization. |
publishDate |
2009 |
dc.date.none.fl_str_mv |
2009-06-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000300026 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000300026 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1516-89132009000300026 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Instituto de Tecnologia do Paraná - Tecpar |
publisher.none.fl_str_mv |
Instituto de Tecnologia do Paraná - Tecpar |
dc.source.none.fl_str_mv |
Brazilian Archives of Biology and Technology v.52 n.3 2009 reponame:Brazilian Archives of Biology and Technology instname:Instituto de Tecnologia do Paraná (Tecpar) instacron:TECPAR |
instname_str |
Instituto de Tecnologia do Paraná (Tecpar) |
instacron_str |
TECPAR |
institution |
TECPAR |
reponame_str |
Brazilian Archives of Biology and Technology |
collection |
Brazilian Archives of Biology and Technology |
repository.name.fl_str_mv |
Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar) |
repository.mail.fl_str_mv |
babt@tecpar.br||babt@tecpar.br |
_version_ |
1750318273113620480 |