Standardized Protocol for In Situ and In Vitro Maintenance of Newly Developed Parthenocarpic Gynoecious Cucumber Inbred
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Archives of Biology and Technology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132022000100414 |
Resumo: | Abstract New parthenocarpic gynoecious cucumber line ‘PBRK-11’ was developed, morphologically characterized and observed that its fruits were dark green, cylindrical, seedless, bitter free, and long (19-22 cm). Besides, different chemicals {GA3, AgNO3, and [Ag(S2O3)2-3]} having different concentrations were used for altering the sex expression of parthenocarpic gynoecious line to induce male flowers for seed production. Analysis of variance showed that chemical applications significantly affect the male flower induction and plant mortality percentage of parthenocarpic gynoecious cucumber inbred. Application of silver thiosulphate @ 250 ppm induced maximum number of male flowers per plant, minimum plant mortality percentage and induced male flowers for longer duration in parthenocarpic gynoecious plants when sprayed at 3-4 leaf stage at weekly interval for three weeks. Besides, in vitro protocol was standardized to maintain it. Shoot tip explants cultured on half MS media showed higher regeneration rate of 53% with highest shoot initiation response in 32 days followed by M5 [MS + BAP (1.0 mgL-1) + Kinetin (1.0 mgL-1)] media with 48% regeneration. Root induction rate (80%) was high in MS media supplemented with IAA (1.0 mgL-1). The parthenocarpic gynoecious expression was found to be stable in tissue culture regenerated progenies. |
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Brazilian Archives of Biology and Technology |
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Standardized Protocol for In Situ and In Vitro Maintenance of Newly Developed Parthenocarpic Gynoecious Cucumber Inbredgynoeciousparthenocarpicsex modificationmicropropagationCucumis sativus L.Abstract New parthenocarpic gynoecious cucumber line ‘PBRK-11’ was developed, morphologically characterized and observed that its fruits were dark green, cylindrical, seedless, bitter free, and long (19-22 cm). Besides, different chemicals {GA3, AgNO3, and [Ag(S2O3)2-3]} having different concentrations were used for altering the sex expression of parthenocarpic gynoecious line to induce male flowers for seed production. Analysis of variance showed that chemical applications significantly affect the male flower induction and plant mortality percentage of parthenocarpic gynoecious cucumber inbred. Application of silver thiosulphate @ 250 ppm induced maximum number of male flowers per plant, minimum plant mortality percentage and induced male flowers for longer duration in parthenocarpic gynoecious plants when sprayed at 3-4 leaf stage at weekly interval for three weeks. Besides, in vitro protocol was standardized to maintain it. Shoot tip explants cultured on half MS media showed higher regeneration rate of 53% with highest shoot initiation response in 32 days followed by M5 [MS + BAP (1.0 mgL-1) + Kinetin (1.0 mgL-1)] media with 48% regeneration. Root induction rate (80%) was high in MS media supplemented with IAA (1.0 mgL-1). The parthenocarpic gynoecious expression was found to be stable in tissue culture regenerated progenies.Instituto de Tecnologia do Paraná - Tecpar2022-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132022000100414Brazilian Archives of Biology and Technology v.65 2022reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/1678-4324-2022200792info:eu-repo/semantics/openAccessDhall,Rajinder KumarHegde,Shreedhari NarasimhaMalhotra,Pawan Kumareng2022-07-20T00:00:00Zoai:scielo:S1516-89132022000100414Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2022-07-20T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false |
dc.title.none.fl_str_mv |
Standardized Protocol for In Situ and In Vitro Maintenance of Newly Developed Parthenocarpic Gynoecious Cucumber Inbred |
title |
Standardized Protocol for In Situ and In Vitro Maintenance of Newly Developed Parthenocarpic Gynoecious Cucumber Inbred |
spellingShingle |
Standardized Protocol for In Situ and In Vitro Maintenance of Newly Developed Parthenocarpic Gynoecious Cucumber Inbred Dhall,Rajinder Kumar gynoecious parthenocarpic sex modification micropropagation Cucumis sativus L. |
title_short |
Standardized Protocol for In Situ and In Vitro Maintenance of Newly Developed Parthenocarpic Gynoecious Cucumber Inbred |
title_full |
Standardized Protocol for In Situ and In Vitro Maintenance of Newly Developed Parthenocarpic Gynoecious Cucumber Inbred |
title_fullStr |
Standardized Protocol for In Situ and In Vitro Maintenance of Newly Developed Parthenocarpic Gynoecious Cucumber Inbred |
title_full_unstemmed |
Standardized Protocol for In Situ and In Vitro Maintenance of Newly Developed Parthenocarpic Gynoecious Cucumber Inbred |
title_sort |
Standardized Protocol for In Situ and In Vitro Maintenance of Newly Developed Parthenocarpic Gynoecious Cucumber Inbred |
author |
Dhall,Rajinder Kumar |
author_facet |
Dhall,Rajinder Kumar Hegde,Shreedhari Narasimha Malhotra,Pawan Kumar |
author_role |
author |
author2 |
Hegde,Shreedhari Narasimha Malhotra,Pawan Kumar |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Dhall,Rajinder Kumar Hegde,Shreedhari Narasimha Malhotra,Pawan Kumar |
dc.subject.por.fl_str_mv |
gynoecious parthenocarpic sex modification micropropagation Cucumis sativus L. |
topic |
gynoecious parthenocarpic sex modification micropropagation Cucumis sativus L. |
description |
Abstract New parthenocarpic gynoecious cucumber line ‘PBRK-11’ was developed, morphologically characterized and observed that its fruits were dark green, cylindrical, seedless, bitter free, and long (19-22 cm). Besides, different chemicals {GA3, AgNO3, and [Ag(S2O3)2-3]} having different concentrations were used for altering the sex expression of parthenocarpic gynoecious line to induce male flowers for seed production. Analysis of variance showed that chemical applications significantly affect the male flower induction and plant mortality percentage of parthenocarpic gynoecious cucumber inbred. Application of silver thiosulphate @ 250 ppm induced maximum number of male flowers per plant, minimum plant mortality percentage and induced male flowers for longer duration in parthenocarpic gynoecious plants when sprayed at 3-4 leaf stage at weekly interval for three weeks. Besides, in vitro protocol was standardized to maintain it. Shoot tip explants cultured on half MS media showed higher regeneration rate of 53% with highest shoot initiation response in 32 days followed by M5 [MS + BAP (1.0 mgL-1) + Kinetin (1.0 mgL-1)] media with 48% regeneration. Root induction rate (80%) was high in MS media supplemented with IAA (1.0 mgL-1). The parthenocarpic gynoecious expression was found to be stable in tissue culture regenerated progenies. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132022000100414 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132022000100414 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/1678-4324-2022200792 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Instituto de Tecnologia do Paraná - Tecpar |
publisher.none.fl_str_mv |
Instituto de Tecnologia do Paraná - Tecpar |
dc.source.none.fl_str_mv |
Brazilian Archives of Biology and Technology v.65 2022 reponame:Brazilian Archives of Biology and Technology instname:Instituto de Tecnologia do Paraná (Tecpar) instacron:TECPAR |
instname_str |
Instituto de Tecnologia do Paraná (Tecpar) |
instacron_str |
TECPAR |
institution |
TECPAR |
reponame_str |
Brazilian Archives of Biology and Technology |
collection |
Brazilian Archives of Biology and Technology |
repository.name.fl_str_mv |
Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar) |
repository.mail.fl_str_mv |
babt@tecpar.br||babt@tecpar.br |
_version_ |
1750318281429876736 |