The Structural Characterization of Extracellular Polysaccharide from Enterococcus faecium M20
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Archives of Biology and Technology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132022000100324 |
Resumo: | Abstract Enterococcus faecium is one of the well-known human pathogens producing biofilm. Todays, it is an important nosocomial infection agent and is spreading rapidly worldwide. As the first step of the infection, this bacterium adheres to tissue or devices with EPS fibrils, and then the biofilm grows. Therefore, investigation of structure of its EPS is extremely important to find a specific way for treatment of its infection. In this study, we isolated and investigated the physicochemical properties extracellular polysaccharide (EPS) from E. faecium M20 obtained from Denizli Public Hospital. The exopolysaccharide of M20 strain was purified by methanol/ethanol extraction method and then analyzed by SAXS, TGA, HPLC and SEM. Carbohydrate and protein were main compounds in the isolated EPS. Moreover, the uronic acid content was found to be high. The HPLC analysis indicated that the EPS consisted of glucose+maltitol, fructose and sorbitol+ksilitol. According to SAXS data, the EPS of M20 possessed a lamellar structure and prolate core shell with a major core radius of 1644.1 Å, a major shell thickness of 121.9 Å, and a bilayer thickness of 12.6 Å. The natural polysaccharide structure was more stable within aqueous (0.5%, w/v) and serum albumin solutions at room temperature. These results may be used to develop new strategies in future for eradicating biofilms of this pathogen bacterium. |
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The Structural Characterization of Extracellular Polysaccharide from Enterococcus faecium M20enterococcus faeciumexopolysaccharideSAXSHPLCSEM.Abstract Enterococcus faecium is one of the well-known human pathogens producing biofilm. Todays, it is an important nosocomial infection agent and is spreading rapidly worldwide. As the first step of the infection, this bacterium adheres to tissue or devices with EPS fibrils, and then the biofilm grows. Therefore, investigation of structure of its EPS is extremely important to find a specific way for treatment of its infection. In this study, we isolated and investigated the physicochemical properties extracellular polysaccharide (EPS) from E. faecium M20 obtained from Denizli Public Hospital. The exopolysaccharide of M20 strain was purified by methanol/ethanol extraction method and then analyzed by SAXS, TGA, HPLC and SEM. Carbohydrate and protein were main compounds in the isolated EPS. Moreover, the uronic acid content was found to be high. The HPLC analysis indicated that the EPS consisted of glucose+maltitol, fructose and sorbitol+ksilitol. According to SAXS data, the EPS of M20 possessed a lamellar structure and prolate core shell with a major core radius of 1644.1 Å, a major shell thickness of 121.9 Å, and a bilayer thickness of 12.6 Å. The natural polysaccharide structure was more stable within aqueous (0.5%, w/v) and serum albumin solutions at room temperature. These results may be used to develop new strategies in future for eradicating biofilms of this pathogen bacterium.Instituto de Tecnologia do Paraná - Tecpar2022-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132022000100324Brazilian Archives of Biology and Technology v.65 2022reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/1678-4324-2022210349info:eu-repo/semantics/openAccessArar,DicleDoğan,Nazime MercanÖzcan,YusufArslan,ŞevkiOrujalipoor,Ilgharİde,Semraeng2022-07-01T00:00:00Zoai:scielo:S1516-89132022000100324Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2022-07-01T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false |
dc.title.none.fl_str_mv |
The Structural Characterization of Extracellular Polysaccharide from Enterococcus faecium M20 |
title |
The Structural Characterization of Extracellular Polysaccharide from Enterococcus faecium M20 |
spellingShingle |
The Structural Characterization of Extracellular Polysaccharide from Enterococcus faecium M20 Arar,Dicle enterococcus faecium exopolysaccharide SAXS HPLC SEM. |
title_short |
The Structural Characterization of Extracellular Polysaccharide from Enterococcus faecium M20 |
title_full |
The Structural Characterization of Extracellular Polysaccharide from Enterococcus faecium M20 |
title_fullStr |
The Structural Characterization of Extracellular Polysaccharide from Enterococcus faecium M20 |
title_full_unstemmed |
The Structural Characterization of Extracellular Polysaccharide from Enterococcus faecium M20 |
title_sort |
The Structural Characterization of Extracellular Polysaccharide from Enterococcus faecium M20 |
author |
Arar,Dicle |
author_facet |
Arar,Dicle Doğan,Nazime Mercan Özcan,Yusuf Arslan,Şevki Orujalipoor,Ilghar İde,Semra |
author_role |
author |
author2 |
Doğan,Nazime Mercan Özcan,Yusuf Arslan,Şevki Orujalipoor,Ilghar İde,Semra |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Arar,Dicle Doğan,Nazime Mercan Özcan,Yusuf Arslan,Şevki Orujalipoor,Ilghar İde,Semra |
dc.subject.por.fl_str_mv |
enterococcus faecium exopolysaccharide SAXS HPLC SEM. |
topic |
enterococcus faecium exopolysaccharide SAXS HPLC SEM. |
description |
Abstract Enterococcus faecium is one of the well-known human pathogens producing biofilm. Todays, it is an important nosocomial infection agent and is spreading rapidly worldwide. As the first step of the infection, this bacterium adheres to tissue or devices with EPS fibrils, and then the biofilm grows. Therefore, investigation of structure of its EPS is extremely important to find a specific way for treatment of its infection. In this study, we isolated and investigated the physicochemical properties extracellular polysaccharide (EPS) from E. faecium M20 obtained from Denizli Public Hospital. The exopolysaccharide of M20 strain was purified by methanol/ethanol extraction method and then analyzed by SAXS, TGA, HPLC and SEM. Carbohydrate and protein were main compounds in the isolated EPS. Moreover, the uronic acid content was found to be high. The HPLC analysis indicated that the EPS consisted of glucose+maltitol, fructose and sorbitol+ksilitol. According to SAXS data, the EPS of M20 possessed a lamellar structure and prolate core shell with a major core radius of 1644.1 Å, a major shell thickness of 121.9 Å, and a bilayer thickness of 12.6 Å. The natural polysaccharide structure was more stable within aqueous (0.5%, w/v) and serum albumin solutions at room temperature. These results may be used to develop new strategies in future for eradicating biofilms of this pathogen bacterium. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132022000100324 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132022000100324 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/1678-4324-2022210349 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Instituto de Tecnologia do Paraná - Tecpar |
publisher.none.fl_str_mv |
Instituto de Tecnologia do Paraná - Tecpar |
dc.source.none.fl_str_mv |
Brazilian Archives of Biology and Technology v.65 2022 reponame:Brazilian Archives of Biology and Technology instname:Instituto de Tecnologia do Paraná (Tecpar) instacron:TECPAR |
instname_str |
Instituto de Tecnologia do Paraná (Tecpar) |
instacron_str |
TECPAR |
institution |
TECPAR |
reponame_str |
Brazilian Archives of Biology and Technology |
collection |
Brazilian Archives of Biology and Technology |
repository.name.fl_str_mv |
Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar) |
repository.mail.fl_str_mv |
babt@tecpar.br||babt@tecpar.br |
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1750318281380593664 |