Proteomic analysis of whey from bovine colostrum and mature milk

Detalhes bibliográficos
Autor(a) principal: Golinelli,Luciana Pacheco
Data de Publicação: 2011
Outros Autores: Conte-Junior,Carlos Adam, Paschoalin,Vânia Margaret Flosi, Silva,Joab Trajano
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Archives of Biology and Technology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000400016
Resumo: The aim of this study was to standardize a methodology to obtain two-dimensional (2D) maps of whey proteins from bovine colostrum and mature milk, using two-dimensional electrophoresis, in order to identify the minor proteins by matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometry (MALDI TOF/TOF MS). A total of 38 proteins were identified, 20 spots in the colostrum whey and 18 in the mature milk whey; 5 of them were identified for the first time.
id TECPAR-1_0a6fac2d3d40661123231f898f8904e1
oai_identifier_str oai:scielo:S1516-89132011000400016
network_acronym_str TECPAR-1
network_name_str Brazilian Archives of Biology and Technology
repository_id_str
spelling Proteomic analysis of whey from bovine colostrum and mature milkbovine whey proteinscolostrum proteinslow-abundance proteinstwo-dimensional electrophoresismass spectrometrymatrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometry (MALDI TOF/TOF MS)The aim of this study was to standardize a methodology to obtain two-dimensional (2D) maps of whey proteins from bovine colostrum and mature milk, using two-dimensional electrophoresis, in order to identify the minor proteins by matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometry (MALDI TOF/TOF MS). A total of 38 proteins were identified, 20 spots in the colostrum whey and 18 in the mature milk whey; 5 of them were identified for the first time.Instituto de Tecnologia do Paraná - Tecpar2011-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000400016Brazilian Archives of Biology and Technology v.54 n.4 2011reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/S1516-89132011000400016info:eu-repo/semantics/openAccessGolinelli,Luciana PachecoConte-Junior,Carlos AdamPaschoalin,Vânia Margaret FlosiSilva,Joab Trajanoeng2011-08-11T00:00:00Zoai:scielo:S1516-89132011000400016Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2011-08-11T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false
dc.title.none.fl_str_mv Proteomic analysis of whey from bovine colostrum and mature milk
title Proteomic analysis of whey from bovine colostrum and mature milk
spellingShingle Proteomic analysis of whey from bovine colostrum and mature milk
Golinelli,Luciana Pacheco
bovine whey proteins
colostrum proteins
low-abundance proteins
two-dimensional electrophoresis
mass spectrometry
matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometry (MALDI TOF/TOF MS)
title_short Proteomic analysis of whey from bovine colostrum and mature milk
title_full Proteomic analysis of whey from bovine colostrum and mature milk
title_fullStr Proteomic analysis of whey from bovine colostrum and mature milk
title_full_unstemmed Proteomic analysis of whey from bovine colostrum and mature milk
title_sort Proteomic analysis of whey from bovine colostrum and mature milk
author Golinelli,Luciana Pacheco
author_facet Golinelli,Luciana Pacheco
Conte-Junior,Carlos Adam
Paschoalin,Vânia Margaret Flosi
Silva,Joab Trajano
author_role author
author2 Conte-Junior,Carlos Adam
Paschoalin,Vânia Margaret Flosi
Silva,Joab Trajano
author2_role author
author
author
dc.contributor.author.fl_str_mv Golinelli,Luciana Pacheco
Conte-Junior,Carlos Adam
Paschoalin,Vânia Margaret Flosi
Silva,Joab Trajano
dc.subject.por.fl_str_mv bovine whey proteins
colostrum proteins
low-abundance proteins
two-dimensional electrophoresis
mass spectrometry
matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometry (MALDI TOF/TOF MS)
topic bovine whey proteins
colostrum proteins
low-abundance proteins
two-dimensional electrophoresis
mass spectrometry
matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometry (MALDI TOF/TOF MS)
description The aim of this study was to standardize a methodology to obtain two-dimensional (2D) maps of whey proteins from bovine colostrum and mature milk, using two-dimensional electrophoresis, in order to identify the minor proteins by matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometry (MALDI TOF/TOF MS). A total of 38 proteins were identified, 20 spots in the colostrum whey and 18 in the mature milk whey; 5 of them were identified for the first time.
publishDate 2011
dc.date.none.fl_str_mv 2011-08-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000400016
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000400016
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1516-89132011000400016
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
dc.source.none.fl_str_mv Brazilian Archives of Biology and Technology v.54 n.4 2011
reponame:Brazilian Archives of Biology and Technology
instname:Instituto de Tecnologia do Paraná (Tecpar)
instacron:TECPAR
instname_str Instituto de Tecnologia do Paraná (Tecpar)
instacron_str TECPAR
institution TECPAR
reponame_str Brazilian Archives of Biology and Technology
collection Brazilian Archives of Biology and Technology
repository.name.fl_str_mv Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)
repository.mail.fl_str_mv babt@tecpar.br||babt@tecpar.br
_version_ 1750318274799730688

Registros relacionados