Cloning of high molecular weight gluten subunit promoter and study on its function in wheat

Detalhes bibliográficos
Autor(a) principal: Weibo,Jin
Data de Publicação: 2009
Outros Autores: Jin,Liu, Fangli,Wu, Aiguang,Guo
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Archives of Biology and Technology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000200002
Resumo: The aim of this work was to study the cloning and characterization of HMW-GS 1Dx2 promoter from Triticum aestivum. A 1050 bp partial promoter fragment including a putative TATA box and 5' encoding sequence of the gene was cloned by amplifying the upstream sequences using the nest-PCR with appropriate primers. The analysis of the promoter sequence against the PLACE (Plant cis-acting Regulatory DNA Elements) database showed the presence of certain putative endosperm-specific regulatory cis-elements in the sequence along with the TATA and CAAT boxes. The histochemical method detected the transient expressions of GUS in the seeds of wheat. The results showed that HMW-GS 1Dx2 promoter had the endosperm-specific transcription activity in the wheat seeds.
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spelling Cloning of high molecular weight gluten subunit promoter and study on its function in wheatTriticum aestivumgluteninpromoterendosperm-specifictransient expressionThe aim of this work was to study the cloning and characterization of HMW-GS 1Dx2 promoter from Triticum aestivum. A 1050 bp partial promoter fragment including a putative TATA box and 5' encoding sequence of the gene was cloned by amplifying the upstream sequences using the nest-PCR with appropriate primers. The analysis of the promoter sequence against the PLACE (Plant cis-acting Regulatory DNA Elements) database showed the presence of certain putative endosperm-specific regulatory cis-elements in the sequence along with the TATA and CAAT boxes. The histochemical method detected the transient expressions of GUS in the seeds of wheat. The results showed that HMW-GS 1Dx2 promoter had the endosperm-specific transcription activity in the wheat seeds.Instituto de Tecnologia do Paraná - Tecpar2009-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000200002Brazilian Archives of Biology and Technology v.52 n.2 2009reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/S1516-89132009000200002info:eu-repo/semantics/openAccessWeibo,JinJin,LiuFangli,WuAiguang,Guoeng2009-05-06T00:00:00Zoai:scielo:S1516-89132009000200002Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2009-05-06T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false
dc.title.none.fl_str_mv Cloning of high molecular weight gluten subunit promoter and study on its function in wheat
title Cloning of high molecular weight gluten subunit promoter and study on its function in wheat
spellingShingle Cloning of high molecular weight gluten subunit promoter and study on its function in wheat
Weibo,Jin
Triticum aestivum
glutenin
promoter
endosperm-specific
transient expression
title_short Cloning of high molecular weight gluten subunit promoter and study on its function in wheat
title_full Cloning of high molecular weight gluten subunit promoter and study on its function in wheat
title_fullStr Cloning of high molecular weight gluten subunit promoter and study on its function in wheat
title_full_unstemmed Cloning of high molecular weight gluten subunit promoter and study on its function in wheat
title_sort Cloning of high molecular weight gluten subunit promoter and study on its function in wheat
author Weibo,Jin
author_facet Weibo,Jin
Jin,Liu
Fangli,Wu
Aiguang,Guo
author_role author
author2 Jin,Liu
Fangli,Wu
Aiguang,Guo
author2_role author
author
author
dc.contributor.author.fl_str_mv Weibo,Jin
Jin,Liu
Fangli,Wu
Aiguang,Guo
dc.subject.por.fl_str_mv Triticum aestivum
glutenin
promoter
endosperm-specific
transient expression
topic Triticum aestivum
glutenin
promoter
endosperm-specific
transient expression
description The aim of this work was to study the cloning and characterization of HMW-GS 1Dx2 promoter from Triticum aestivum. A 1050 bp partial promoter fragment including a putative TATA box and 5' encoding sequence of the gene was cloned by amplifying the upstream sequences using the nest-PCR with appropriate primers. The analysis of the promoter sequence against the PLACE (Plant cis-acting Regulatory DNA Elements) database showed the presence of certain putative endosperm-specific regulatory cis-elements in the sequence along with the TATA and CAAT boxes. The histochemical method detected the transient expressions of GUS in the seeds of wheat. The results showed that HMW-GS 1Dx2 promoter had the endosperm-specific transcription activity in the wheat seeds.
publishDate 2009
dc.date.none.fl_str_mv 2009-04-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000200002
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000200002
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1516-89132009000200002
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
dc.source.none.fl_str_mv Brazilian Archives of Biology and Technology v.52 n.2 2009
reponame:Brazilian Archives of Biology and Technology
instname:Instituto de Tecnologia do Paraná (Tecpar)
instacron:TECPAR
instname_str Instituto de Tecnologia do Paraná (Tecpar)
instacron_str TECPAR
institution TECPAR
reponame_str Brazilian Archives of Biology and Technology
collection Brazilian Archives of Biology and Technology
repository.name.fl_str_mv Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)
repository.mail.fl_str_mv babt@tecpar.br||babt@tecpar.br
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