Improvement of xylanase production by a parasexual cross between Aspergillus niger strains

Detalhes bibliográficos
Autor(a) principal: Loera,Octavio
Data de Publicação: 2003
Outros Autores: Córdova,Jesús
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Archives of Biology and Technology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000200006
Resumo: A diploid strain (D4) isolated via parasexual recombination between two Aspergillus niger xylanase overproducing mutants was characterised in terms of enzyme production and catabolite repression by glucose. This strain increased xylanase production (607 nkat/ml), which was nearly 100% higher than titers achieved by the wild type strain (305 nkat/ml) and 28% higher than the best mutant used to induce parasexual cycle. Diploid D4 was also less sensitive to carbon catabolite repression by glucose, since xylanolytic activity was detected under conditions normally repressing production by the wild type strain. No decrease in maximal xylanase levels was observed in the presence of glucose for diploid D4.
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spelling Improvement of xylanase production by a parasexual cross between Aspergillus niger strainsCarbon repressionxylanase productionmutantsparasexual crossA diploid strain (D4) isolated via parasexual recombination between two Aspergillus niger xylanase overproducing mutants was characterised in terms of enzyme production and catabolite repression by glucose. This strain increased xylanase production (607 nkat/ml), which was nearly 100% higher than titers achieved by the wild type strain (305 nkat/ml) and 28% higher than the best mutant used to induce parasexual cycle. Diploid D4 was also less sensitive to carbon catabolite repression by glucose, since xylanolytic activity was detected under conditions normally repressing production by the wild type strain. No decrease in maximal xylanase levels was observed in the presence of glucose for diploid D4.Instituto de Tecnologia do Paraná - Tecpar2003-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000200006Brazilian Archives of Biology and Technology v.46 n.2 2003reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/S1516-89132003000200006info:eu-repo/semantics/openAccessLoera,OctavioCórdova,Jesúseng2003-07-29T00:00:00Zoai:scielo:S1516-89132003000200006Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2003-07-29T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false
dc.title.none.fl_str_mv Improvement of xylanase production by a parasexual cross between Aspergillus niger strains
title Improvement of xylanase production by a parasexual cross between Aspergillus niger strains
spellingShingle Improvement of xylanase production by a parasexual cross between Aspergillus niger strains
Loera,Octavio
Carbon repression
xylanase production
mutants
parasexual cross
title_short Improvement of xylanase production by a parasexual cross between Aspergillus niger strains
title_full Improvement of xylanase production by a parasexual cross between Aspergillus niger strains
title_fullStr Improvement of xylanase production by a parasexual cross between Aspergillus niger strains
title_full_unstemmed Improvement of xylanase production by a parasexual cross between Aspergillus niger strains
title_sort Improvement of xylanase production by a parasexual cross between Aspergillus niger strains
author Loera,Octavio
author_facet Loera,Octavio
Córdova,Jesús
author_role author
author2 Córdova,Jesús
author2_role author
dc.contributor.author.fl_str_mv Loera,Octavio
Córdova,Jesús
dc.subject.por.fl_str_mv Carbon repression
xylanase production
mutants
parasexual cross
topic Carbon repression
xylanase production
mutants
parasexual cross
description A diploid strain (D4) isolated via parasexual recombination between two Aspergillus niger xylanase overproducing mutants was characterised in terms of enzyme production and catabolite repression by glucose. This strain increased xylanase production (607 nkat/ml), which was nearly 100% higher than titers achieved by the wild type strain (305 nkat/ml) and 28% higher than the best mutant used to induce parasexual cycle. Diploid D4 was also less sensitive to carbon catabolite repression by glucose, since xylanolytic activity was detected under conditions normally repressing production by the wild type strain. No decrease in maximal xylanase levels was observed in the presence of glucose for diploid D4.
publishDate 2003
dc.date.none.fl_str_mv 2003-03-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000200006
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000200006
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1516-89132003000200006
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
dc.source.none.fl_str_mv Brazilian Archives of Biology and Technology v.46 n.2 2003
reponame:Brazilian Archives of Biology and Technology
instname:Instituto de Tecnologia do Paraná (Tecpar)
instacron:TECPAR
instname_str Instituto de Tecnologia do Paraná (Tecpar)
instacron_str TECPAR
institution TECPAR
reponame_str Brazilian Archives of Biology and Technology
collection Brazilian Archives of Biology and Technology
repository.name.fl_str_mv Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)
repository.mail.fl_str_mv babt@tecpar.br||babt@tecpar.br
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