Production and characterization of exopolysaccharides (EPS) from biofilm forming marine bacterium

Detalhes bibliográficos
Autor(a) principal: Kumar,Muthusamy Ashok
Data de Publicação: 2011
Outros Autores: Anandapandian,Kanapathi Thangavel Kasirajan, Parthiban,Karuppiah
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Archives of Biology and Technology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000200006
Resumo: Marine water was used for the biofilm forming bacterial isolation. A biofilm forming device was made by using PVC pipes with sterile glass slides. Five morphologically different bacteria were isolated from the scrapping of glass slides from the device after a month period of time. The efficiency of EPS production was checked for all the isolates. Using scanning electron microscope (SEM), the morphology of microbial cells and colonies was studied. The maximum EPS was produced by the strain B3, which was analyzed and characterized by thin layer chromatography (TLC) and Fourier transform infrared spectroscopy (FTIR) spectra. B3 EPS displayed a strong absorption band of - OH at 3415.31 cm-1 and COOH at 1631.48 cm-1 showing it to be polysaccharide.
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spelling Production and characterization of exopolysaccharides (EPS) from biofilm forming marine bacteriumBiofilmMarine BacteriaEPSSEMTLC and FTIRMarine water was used for the biofilm forming bacterial isolation. A biofilm forming device was made by using PVC pipes with sterile glass slides. Five morphologically different bacteria were isolated from the scrapping of glass slides from the device after a month period of time. The efficiency of EPS production was checked for all the isolates. Using scanning electron microscope (SEM), the morphology of microbial cells and colonies was studied. The maximum EPS was produced by the strain B3, which was analyzed and characterized by thin layer chromatography (TLC) and Fourier transform infrared spectroscopy (FTIR) spectra. B3 EPS displayed a strong absorption band of - OH at 3415.31 cm-1 and COOH at 1631.48 cm-1 showing it to be polysaccharide.Instituto de Tecnologia do Paraná - Tecpar2011-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000200006Brazilian Archives of Biology and Technology v.54 n.2 2011reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/S1516-89132011000200006info:eu-repo/semantics/openAccessKumar,Muthusamy AshokAnandapandian,Kanapathi Thangavel KasirajanParthiban,Karuppiaheng2011-04-15T00:00:00Zoai:scielo:S1516-89132011000200006Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2011-04-15T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false
dc.title.none.fl_str_mv Production and characterization of exopolysaccharides (EPS) from biofilm forming marine bacterium
title Production and characterization of exopolysaccharides (EPS) from biofilm forming marine bacterium
spellingShingle Production and characterization of exopolysaccharides (EPS) from biofilm forming marine bacterium
Kumar,Muthusamy Ashok
Biofilm
Marine Bacteria
EPS
SEM
TLC and FTIR
title_short Production and characterization of exopolysaccharides (EPS) from biofilm forming marine bacterium
title_full Production and characterization of exopolysaccharides (EPS) from biofilm forming marine bacterium
title_fullStr Production and characterization of exopolysaccharides (EPS) from biofilm forming marine bacterium
title_full_unstemmed Production and characterization of exopolysaccharides (EPS) from biofilm forming marine bacterium
title_sort Production and characterization of exopolysaccharides (EPS) from biofilm forming marine bacterium
author Kumar,Muthusamy Ashok
author_facet Kumar,Muthusamy Ashok
Anandapandian,Kanapathi Thangavel Kasirajan
Parthiban,Karuppiah
author_role author
author2 Anandapandian,Kanapathi Thangavel Kasirajan
Parthiban,Karuppiah
author2_role author
author
dc.contributor.author.fl_str_mv Kumar,Muthusamy Ashok
Anandapandian,Kanapathi Thangavel Kasirajan
Parthiban,Karuppiah
dc.subject.por.fl_str_mv Biofilm
Marine Bacteria
EPS
SEM
TLC and FTIR
topic Biofilm
Marine Bacteria
EPS
SEM
TLC and FTIR
description Marine water was used for the biofilm forming bacterial isolation. A biofilm forming device was made by using PVC pipes with sterile glass slides. Five morphologically different bacteria were isolated from the scrapping of glass slides from the device after a month period of time. The efficiency of EPS production was checked for all the isolates. Using scanning electron microscope (SEM), the morphology of microbial cells and colonies was studied. The maximum EPS was produced by the strain B3, which was analyzed and characterized by thin layer chromatography (TLC) and Fourier transform infrared spectroscopy (FTIR) spectra. B3 EPS displayed a strong absorption band of - OH at 3415.31 cm-1 and COOH at 1631.48 cm-1 showing it to be polysaccharide.
publishDate 2011
dc.date.none.fl_str_mv 2011-04-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000200006
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000200006
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1516-89132011000200006
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
dc.source.none.fl_str_mv Brazilian Archives of Biology and Technology v.54 n.2 2011
reponame:Brazilian Archives of Biology and Technology
instname:Instituto de Tecnologia do Paraná (Tecpar)
instacron:TECPAR
instname_str Instituto de Tecnologia do Paraná (Tecpar)
instacron_str TECPAR
institution TECPAR
reponame_str Brazilian Archives of Biology and Technology
collection Brazilian Archives of Biology and Technology
repository.name.fl_str_mv Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)
repository.mail.fl_str_mv babt@tecpar.br||babt@tecpar.br
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