An inexpensive alternative equipment for the plant material embedding in the paraffin under the vacuum

Detalhes bibliográficos
Autor(a) principal: Leitão,Carlos André Espolador
Data de Publicação: 2008
Outros Autores: Cortelazzo,Angelo Luiz
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Archives of Biology and Technology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000500018
Resumo: The present work describes an equipment constructed using inexpensive material for embedding the plant material in the paraffin under the vacuum, using an oven and a vacuum pump. The equipment was tested using the samples of Rodriguezia venusta (Orchidaceae) buds embedded in paraffin, where half of the samples were submitted to the vacuum by the equipment during the embedding. The material was sectioned with a rotary microtome, obtaining full series of quality sections. The control was hard to section with the microtome, obtaining damaged sections due the air bubbles, making the ribbon formation difficult. These results proved the effectiveness of the equipment, making it a practical, inexpensive and more portable solution for newly established laboratories.
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spelling An inexpensive alternative equipment for the plant material embedding in the paraffin under the vacuumEmbeddingparaffin methodplant anatomytechnique in microscopyThe present work describes an equipment constructed using inexpensive material for embedding the plant material in the paraffin under the vacuum, using an oven and a vacuum pump. The equipment was tested using the samples of Rodriguezia venusta (Orchidaceae) buds embedded in paraffin, where half of the samples were submitted to the vacuum by the equipment during the embedding. The material was sectioned with a rotary microtome, obtaining full series of quality sections. The control was hard to section with the microtome, obtaining damaged sections due the air bubbles, making the ribbon formation difficult. These results proved the effectiveness of the equipment, making it a practical, inexpensive and more portable solution for newly established laboratories.Instituto de Tecnologia do Paraná - Tecpar2008-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000500018Brazilian Archives of Biology and Technology v.51 n.5 2008reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/S1516-89132008000500018info:eu-repo/semantics/openAccessLeitão,Carlos André EspoladorCortelazzo,Angelo Luizeng2008-10-29T00:00:00Zoai:scielo:S1516-89132008000500018Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2008-10-29T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false
dc.title.none.fl_str_mv An inexpensive alternative equipment for the plant material embedding in the paraffin under the vacuum
title An inexpensive alternative equipment for the plant material embedding in the paraffin under the vacuum
spellingShingle An inexpensive alternative equipment for the plant material embedding in the paraffin under the vacuum
Leitão,Carlos André Espolador
Embedding
paraffin method
plant anatomy
technique in microscopy
title_short An inexpensive alternative equipment for the plant material embedding in the paraffin under the vacuum
title_full An inexpensive alternative equipment for the plant material embedding in the paraffin under the vacuum
title_fullStr An inexpensive alternative equipment for the plant material embedding in the paraffin under the vacuum
title_full_unstemmed An inexpensive alternative equipment for the plant material embedding in the paraffin under the vacuum
title_sort An inexpensive alternative equipment for the plant material embedding in the paraffin under the vacuum
author Leitão,Carlos André Espolador
author_facet Leitão,Carlos André Espolador
Cortelazzo,Angelo Luiz
author_role author
author2 Cortelazzo,Angelo Luiz
author2_role author
dc.contributor.author.fl_str_mv Leitão,Carlos André Espolador
Cortelazzo,Angelo Luiz
dc.subject.por.fl_str_mv Embedding
paraffin method
plant anatomy
technique in microscopy
topic Embedding
paraffin method
plant anatomy
technique in microscopy
description The present work describes an equipment constructed using inexpensive material for embedding the plant material in the paraffin under the vacuum, using an oven and a vacuum pump. The equipment was tested using the samples of Rodriguezia venusta (Orchidaceae) buds embedded in paraffin, where half of the samples were submitted to the vacuum by the equipment during the embedding. The material was sectioned with a rotary microtome, obtaining full series of quality sections. The control was hard to section with the microtome, obtaining damaged sections due the air bubbles, making the ribbon formation difficult. These results proved the effectiveness of the equipment, making it a practical, inexpensive and more portable solution for newly established laboratories.
publishDate 2008
dc.date.none.fl_str_mv 2008-10-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000500018
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000500018
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1516-89132008000500018
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
dc.source.none.fl_str_mv Brazilian Archives of Biology and Technology v.51 n.5 2008
reponame:Brazilian Archives of Biology and Technology
instname:Instituto de Tecnologia do Paraná (Tecpar)
instacron:TECPAR
instname_str Instituto de Tecnologia do Paraná (Tecpar)
instacron_str TECPAR
institution TECPAR
reponame_str Brazilian Archives of Biology and Technology
collection Brazilian Archives of Biology and Technology
repository.name.fl_str_mv Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)
repository.mail.fl_str_mv babt@tecpar.br||babt@tecpar.br
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