Liquid-liquid extraction of enzymes by affinity aqueous two-phase systems

Detalhes bibliográficos
Autor(a) principal: Yan,Xu
Data de Publicação: 2003
Outros Autores: Souza,Maria Aparecida, Pontes,Marcela Z. R., Vitolo,Michele, Pessoa Júnior,Adalberto
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Archives of Biology and Technology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000400030
Resumo: From analytical to commercial scale, aqueous two-phase systems have their application in the purification, characterization and study of biomaterials. In order to improve the selectivity of the systems, the biospecific affinity ligands were introduced. In the affinity partitioning aqueous two-phase system, have many enzymes been purified. This review discusses the partitioning of some enzymes in the affinity aqueous two-phase systems in regard to the different ligands, including reactive dyes, metal ions and other ligands. Some integration of aqueous two-phase system with other techniques for more effective purification of enzymes are also presented.
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spelling Liquid-liquid extraction of enzymes by affinity aqueous two-phase systemsAffinity partitioningAqueous two-phase systemsReactive dyesLigandsEnzymesFrom analytical to commercial scale, aqueous two-phase systems have their application in the purification, characterization and study of biomaterials. In order to improve the selectivity of the systems, the biospecific affinity ligands were introduced. In the affinity partitioning aqueous two-phase system, have many enzymes been purified. This review discusses the partitioning of some enzymes in the affinity aqueous two-phase systems in regard to the different ligands, including reactive dyes, metal ions and other ligands. Some integration of aqueous two-phase system with other techniques for more effective purification of enzymes are also presented.Instituto de Tecnologia do Paraná - Tecpar2003-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000400030Brazilian Archives of Biology and Technology v.46 n.4 2003reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/S1516-89132003000400030info:eu-repo/semantics/openAccessYan,XuSouza,Maria AparecidaPontes,Marcela Z. R.Vitolo,MichelePessoa Júnior,Adalbertoeng2004-02-10T00:00:00Zoai:scielo:S1516-89132003000400030Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2004-02-10T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false
dc.title.none.fl_str_mv Liquid-liquid extraction of enzymes by affinity aqueous two-phase systems
title Liquid-liquid extraction of enzymes by affinity aqueous two-phase systems
spellingShingle Liquid-liquid extraction of enzymes by affinity aqueous two-phase systems
Yan,Xu
Affinity partitioning
Aqueous two-phase systems
Reactive dyes
Ligands
Enzymes
title_short Liquid-liquid extraction of enzymes by affinity aqueous two-phase systems
title_full Liquid-liquid extraction of enzymes by affinity aqueous two-phase systems
title_fullStr Liquid-liquid extraction of enzymes by affinity aqueous two-phase systems
title_full_unstemmed Liquid-liquid extraction of enzymes by affinity aqueous two-phase systems
title_sort Liquid-liquid extraction of enzymes by affinity aqueous two-phase systems
author Yan,Xu
author_facet Yan,Xu
Souza,Maria Aparecida
Pontes,Marcela Z. R.
Vitolo,Michele
Pessoa Júnior,Adalberto
author_role author
author2 Souza,Maria Aparecida
Pontes,Marcela Z. R.
Vitolo,Michele
Pessoa Júnior,Adalberto
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Yan,Xu
Souza,Maria Aparecida
Pontes,Marcela Z. R.
Vitolo,Michele
Pessoa Júnior,Adalberto
dc.subject.por.fl_str_mv Affinity partitioning
Aqueous two-phase systems
Reactive dyes
Ligands
Enzymes
topic Affinity partitioning
Aqueous two-phase systems
Reactive dyes
Ligands
Enzymes
description From analytical to commercial scale, aqueous two-phase systems have their application in the purification, characterization and study of biomaterials. In order to improve the selectivity of the systems, the biospecific affinity ligands were introduced. In the affinity partitioning aqueous two-phase system, have many enzymes been purified. This review discusses the partitioning of some enzymes in the affinity aqueous two-phase systems in regard to the different ligands, including reactive dyes, metal ions and other ligands. Some integration of aqueous two-phase system with other techniques for more effective purification of enzymes are also presented.
publishDate 2003
dc.date.none.fl_str_mv 2003-12-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000400030
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000400030
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1516-89132003000400030
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
dc.source.none.fl_str_mv Brazilian Archives of Biology and Technology v.46 n.4 2003
reponame:Brazilian Archives of Biology and Technology
instname:Instituto de Tecnologia do Paraná (Tecpar)
instacron:TECPAR
instname_str Instituto de Tecnologia do Paraná (Tecpar)
instacron_str TECPAR
institution TECPAR
reponame_str Brazilian Archives of Biology and Technology
collection Brazilian Archives of Biology and Technology
repository.name.fl_str_mv Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)
repository.mail.fl_str_mv babt@tecpar.br||babt@tecpar.br
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