Effect of Salicylic Acid, 2,4-D and 2i-P on the Production of Secondary Metabolites in Garcinia brasiliensis Mart. Callus

• Autor(a) principal: Teixeira,Maria Gessi
• Data de Publicação: 2019
• Outros Autores: Carvalho,Marília, Leite,Marco Aurélio, Barbosa,Sandro, Santos filho,Plinio Rodrigues dos, Santos,Breno Régis
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Brazilian Archives of Biology and Technology
• Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100407
• topic: Bacupari; phenols; flavonoids; 7-epiclusianone; fukugetin

Abstract The aim of this work was to evaluate the effect of Salicylic acid, 2,4-D and 2-iP on the production of total phenolic and flavonoids as well in the levels of fukugetin and 7-epiclusianone in callus of Garcinia brasiliensis zygotic embryos. For this, Bacupari callus were exposed to different concentrations (0.0; 0.1; 1; 10; 100 µM) of Salicylic acid (SA) in the presence or in the absence of 72 μM 2-iP and 28.73 μM 2,4-D. The highest concentration of total phenolic occurred in the treatment with 100 μM SA in callus subcultured in the absence of 2,4-D and 2-iP, and with 10 μM SA in callus subcultured in the presence of these regulators. Concerning flavonoids, 2,4-D and 2-iP supplementation without the presence of the SA was sufficient for the highest levels. Additionally, it was possible to identify the fukugetin and 7-epiclusianone. However, the variation in the levels was very high, especially for the fukugetin. Therefore, in most treatments, there was no statistically difference. Except for the treatment with 10 μM SA + 2,4-D and 2-iP, where there was a significant increase in 7-epiclusianone.