Non-linear dynamics of chromosome condensation induced by colcemid

Detalhes bibliográficos
Autor(a) principal: Fernandes,Thiago de Salazar e
Data de Publicação: 2013
Outros Autores: Silva,Isvânia Maria Serafim da, Moraes,Renato Barros, Aguiar,Leandro Álvaro de Alcântara, Amaral,Ademir, Barbosa,Catão Temístocles, Nogueira,Romildo de Albuquerque
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Archives of Biology and Technology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000100011
Resumo: This study investigated the dynamical process of chromosome condensation after colcemid treatment. Two pairs of human chromosomes, #2 and #3, were highlighted for the accurate identification by fluorescence in situ hybridization (FISH). A computerized image analysis system was used to measure the lengths of the two pairs of chromosomes averaged over 50 metaphases of different cultures with colcemid (0.5 µg/mL) added either at 3 or 48 h of a total 72 h culture period. For determining whether the process of chromosome condensation was chaotic or random, the algorithm of Detrended Fluctuation Analysis (DFA) was used. In order to evaluate the power of the method, the data were shuffled and DFA was performed again. It was found that colcemid prolonged treatment induced a significantly greater chromosome condensation (p<0.05), and the dynamics of this process was determined by the DFA and showed to be chaotic, with scaling exponents with range values 0.5< α<1.0. When the data were shuffled, the scaling exponent αreduced around to 0.5, which was characteristic of random events. These findings reinforced the idea that colcemid could interfere in some manner with the structure of chromosomes and the dynamics of chromosome condensation was non-linear.
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spelling Non-linear dynamics of chromosome condensation induced by colcemidNon-linear dynamicschaoschromosome condensationcolcemidThis study investigated the dynamical process of chromosome condensation after colcemid treatment. Two pairs of human chromosomes, #2 and #3, were highlighted for the accurate identification by fluorescence in situ hybridization (FISH). A computerized image analysis system was used to measure the lengths of the two pairs of chromosomes averaged over 50 metaphases of different cultures with colcemid (0.5 µg/mL) added either at 3 or 48 h of a total 72 h culture period. For determining whether the process of chromosome condensation was chaotic or random, the algorithm of Detrended Fluctuation Analysis (DFA) was used. In order to evaluate the power of the method, the data were shuffled and DFA was performed again. It was found that colcemid prolonged treatment induced a significantly greater chromosome condensation (p<0.05), and the dynamics of this process was determined by the DFA and showed to be chaotic, with scaling exponents with range values 0.5< α<1.0. When the data were shuffled, the scaling exponent αreduced around to 0.5, which was characteristic of random events. These findings reinforced the idea that colcemid could interfere in some manner with the structure of chromosomes and the dynamics of chromosome condensation was non-linear.Instituto de Tecnologia do Paraná - Tecpar2013-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000100011Brazilian Archives of Biology and Technology v.56 n.1 2013reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/S1516-89132013000100011info:eu-repo/semantics/openAccessFernandes,Thiago de Salazar eSilva,Isvânia Maria Serafim daMoraes,Renato BarrosAguiar,Leandro Álvaro de AlcântaraAmaral,AdemirBarbosa,Catão TemístoclesNogueira,Romildo de Albuquerqueeng2013-04-09T00:00:00Zoai:scielo:S1516-89132013000100011Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2013-04-09T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false
dc.title.none.fl_str_mv Non-linear dynamics of chromosome condensation induced by colcemid
title Non-linear dynamics of chromosome condensation induced by colcemid
spellingShingle Non-linear dynamics of chromosome condensation induced by colcemid
Fernandes,Thiago de Salazar e
Non-linear dynamics
chaos
chromosome condensation
colcemid
title_short Non-linear dynamics of chromosome condensation induced by colcemid
title_full Non-linear dynamics of chromosome condensation induced by colcemid
title_fullStr Non-linear dynamics of chromosome condensation induced by colcemid
title_full_unstemmed Non-linear dynamics of chromosome condensation induced by colcemid
title_sort Non-linear dynamics of chromosome condensation induced by colcemid
author Fernandes,Thiago de Salazar e
author_facet Fernandes,Thiago de Salazar e
Silva,Isvânia Maria Serafim da
Moraes,Renato Barros
Aguiar,Leandro Álvaro de Alcântara
Amaral,Ademir
Barbosa,Catão Temístocles
Nogueira,Romildo de Albuquerque
author_role author
author2 Silva,Isvânia Maria Serafim da
Moraes,Renato Barros
Aguiar,Leandro Álvaro de Alcântara
Amaral,Ademir
Barbosa,Catão Temístocles
Nogueira,Romildo de Albuquerque
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Fernandes,Thiago de Salazar e
Silva,Isvânia Maria Serafim da
Moraes,Renato Barros
Aguiar,Leandro Álvaro de Alcântara
Amaral,Ademir
Barbosa,Catão Temístocles
Nogueira,Romildo de Albuquerque
dc.subject.por.fl_str_mv Non-linear dynamics
chaos
chromosome condensation
colcemid
topic Non-linear dynamics
chaos
chromosome condensation
colcemid
description This study investigated the dynamical process of chromosome condensation after colcemid treatment. Two pairs of human chromosomes, #2 and #3, were highlighted for the accurate identification by fluorescence in situ hybridization (FISH). A computerized image analysis system was used to measure the lengths of the two pairs of chromosomes averaged over 50 metaphases of different cultures with colcemid (0.5 µg/mL) added either at 3 or 48 h of a total 72 h culture period. For determining whether the process of chromosome condensation was chaotic or random, the algorithm of Detrended Fluctuation Analysis (DFA) was used. In order to evaluate the power of the method, the data were shuffled and DFA was performed again. It was found that colcemid prolonged treatment induced a significantly greater chromosome condensation (p<0.05), and the dynamics of this process was determined by the DFA and showed to be chaotic, with scaling exponents with range values 0.5< α<1.0. When the data were shuffled, the scaling exponent αreduced around to 0.5, which was characteristic of random events. These findings reinforced the idea that colcemid could interfere in some manner with the structure of chromosomes and the dynamics of chromosome condensation was non-linear.
publishDate 2013
dc.date.none.fl_str_mv 2013-02-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000100011
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000100011
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1516-89132013000100011
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
dc.source.none.fl_str_mv Brazilian Archives of Biology and Technology v.56 n.1 2013
reponame:Brazilian Archives of Biology and Technology
instname:Instituto de Tecnologia do Paraná (Tecpar)
instacron:TECPAR
instname_str Instituto de Tecnologia do Paraná (Tecpar)
instacron_str TECPAR
institution TECPAR
reponame_str Brazilian Archives of Biology and Technology
collection Brazilian Archives of Biology and Technology
repository.name.fl_str_mv Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)
repository.mail.fl_str_mv babt@tecpar.br||babt@tecpar.br
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