Non-linear dynamics of chromosome condensation induced by colcemid
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Archives of Biology and Technology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000100011 |
Resumo: | This study investigated the dynamical process of chromosome condensation after colcemid treatment. Two pairs of human chromosomes, #2 and #3, were highlighted for the accurate identification by fluorescence in situ hybridization (FISH). A computerized image analysis system was used to measure the lengths of the two pairs of chromosomes averaged over 50 metaphases of different cultures with colcemid (0.5 µg/mL) added either at 3 or 48 h of a total 72 h culture period. For determining whether the process of chromosome condensation was chaotic or random, the algorithm of Detrended Fluctuation Analysis (DFA) was used. In order to evaluate the power of the method, the data were shuffled and DFA was performed again. It was found that colcemid prolonged treatment induced a significantly greater chromosome condensation (p<0.05), and the dynamics of this process was determined by the DFA and showed to be chaotic, with scaling exponents with range values 0.5< α<1.0. When the data were shuffled, the scaling exponent αreduced around to 0.5, which was characteristic of random events. These findings reinforced the idea that colcemid could interfere in some manner with the structure of chromosomes and the dynamics of chromosome condensation was non-linear. |
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Brazilian Archives of Biology and Technology |
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Non-linear dynamics of chromosome condensation induced by colcemidNon-linear dynamicschaoschromosome condensationcolcemidThis study investigated the dynamical process of chromosome condensation after colcemid treatment. Two pairs of human chromosomes, #2 and #3, were highlighted for the accurate identification by fluorescence in situ hybridization (FISH). A computerized image analysis system was used to measure the lengths of the two pairs of chromosomes averaged over 50 metaphases of different cultures with colcemid (0.5 µg/mL) added either at 3 or 48 h of a total 72 h culture period. For determining whether the process of chromosome condensation was chaotic or random, the algorithm of Detrended Fluctuation Analysis (DFA) was used. In order to evaluate the power of the method, the data were shuffled and DFA was performed again. It was found that colcemid prolonged treatment induced a significantly greater chromosome condensation (p<0.05), and the dynamics of this process was determined by the DFA and showed to be chaotic, with scaling exponents with range values 0.5< α<1.0. When the data were shuffled, the scaling exponent αreduced around to 0.5, which was characteristic of random events. These findings reinforced the idea that colcemid could interfere in some manner with the structure of chromosomes and the dynamics of chromosome condensation was non-linear.Instituto de Tecnologia do Paraná - Tecpar2013-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000100011Brazilian Archives of Biology and Technology v.56 n.1 2013reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/S1516-89132013000100011info:eu-repo/semantics/openAccessFernandes,Thiago de Salazar eSilva,Isvânia Maria Serafim daMoraes,Renato BarrosAguiar,Leandro Álvaro de AlcântaraAmaral,AdemirBarbosa,Catão TemístoclesNogueira,Romildo de Albuquerqueeng2013-04-09T00:00:00Zoai:scielo:S1516-89132013000100011Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2013-04-09T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false |
dc.title.none.fl_str_mv |
Non-linear dynamics of chromosome condensation induced by colcemid |
title |
Non-linear dynamics of chromosome condensation induced by colcemid |
spellingShingle |
Non-linear dynamics of chromosome condensation induced by colcemid Fernandes,Thiago de Salazar e Non-linear dynamics chaos chromosome condensation colcemid |
title_short |
Non-linear dynamics of chromosome condensation induced by colcemid |
title_full |
Non-linear dynamics of chromosome condensation induced by colcemid |
title_fullStr |
Non-linear dynamics of chromosome condensation induced by colcemid |
title_full_unstemmed |
Non-linear dynamics of chromosome condensation induced by colcemid |
title_sort |
Non-linear dynamics of chromosome condensation induced by colcemid |
author |
Fernandes,Thiago de Salazar e |
author_facet |
Fernandes,Thiago de Salazar e Silva,Isvânia Maria Serafim da Moraes,Renato Barros Aguiar,Leandro Álvaro de Alcântara Amaral,Ademir Barbosa,Catão Temístocles Nogueira,Romildo de Albuquerque |
author_role |
author |
author2 |
Silva,Isvânia Maria Serafim da Moraes,Renato Barros Aguiar,Leandro Álvaro de Alcântara Amaral,Ademir Barbosa,Catão Temístocles Nogueira,Romildo de Albuquerque |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Fernandes,Thiago de Salazar e Silva,Isvânia Maria Serafim da Moraes,Renato Barros Aguiar,Leandro Álvaro de Alcântara Amaral,Ademir Barbosa,Catão Temístocles Nogueira,Romildo de Albuquerque |
dc.subject.por.fl_str_mv |
Non-linear dynamics chaos chromosome condensation colcemid |
topic |
Non-linear dynamics chaos chromosome condensation colcemid |
description |
This study investigated the dynamical process of chromosome condensation after colcemid treatment. Two pairs of human chromosomes, #2 and #3, were highlighted for the accurate identification by fluorescence in situ hybridization (FISH). A computerized image analysis system was used to measure the lengths of the two pairs of chromosomes averaged over 50 metaphases of different cultures with colcemid (0.5 µg/mL) added either at 3 or 48 h of a total 72 h culture period. For determining whether the process of chromosome condensation was chaotic or random, the algorithm of Detrended Fluctuation Analysis (DFA) was used. In order to evaluate the power of the method, the data were shuffled and DFA was performed again. It was found that colcemid prolonged treatment induced a significantly greater chromosome condensation (p<0.05), and the dynamics of this process was determined by the DFA and showed to be chaotic, with scaling exponents with range values 0.5< α<1.0. When the data were shuffled, the scaling exponent αreduced around to 0.5, which was characteristic of random events. These findings reinforced the idea that colcemid could interfere in some manner with the structure of chromosomes and the dynamics of chromosome condensation was non-linear. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-02-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000100011 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000100011 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1516-89132013000100011 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Instituto de Tecnologia do Paraná - Tecpar |
publisher.none.fl_str_mv |
Instituto de Tecnologia do Paraná - Tecpar |
dc.source.none.fl_str_mv |
Brazilian Archives of Biology and Technology v.56 n.1 2013 reponame:Brazilian Archives of Biology and Technology instname:Instituto de Tecnologia do Paraná (Tecpar) instacron:TECPAR |
instname_str |
Instituto de Tecnologia do Paraná (Tecpar) |
instacron_str |
TECPAR |
institution |
TECPAR |
reponame_str |
Brazilian Archives of Biology and Technology |
collection |
Brazilian Archives of Biology and Technology |
repository.name.fl_str_mv |
Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar) |
repository.mail.fl_str_mv |
babt@tecpar.br||babt@tecpar.br |
_version_ |
1750318275589308416 |