High quality DNA from human papillomavirus (HPV) for PCR/RFLPs

Detalhes bibliográficos
Autor(a) principal: Wanderlei-Silva,Denise
Data de Publicação: 2005
Outros Autores: Nobre,Mariana, Gonzaga,Rosa Silva, Viana,Luciana Silva, Ramalho Neto,Eduardo
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Archives of Biology and Technology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000100006
Resumo: The analysis of DNA in clinical samples for a secure diagnostic has become indispensable nowadays. Techniques approaching isolation of high molecular weigth DNA of HPV could lead to efficient amplification and early clinical diagnosis of the virus DNA by PCR (polymerase chain reaction). We describe a fast, non-toxical, efficient and cheap method for DNA isolation of human papilloma virus (HPV) from cervical smears using guanidine (DNAzol solution). A 450 bp DNA band correponding to the late region (L1) of the virus genome was detected by PCR, showing that the DNAzol extraction soluction generated a good viral DNA yield. The electrophoretic pattern after digestion with restriction endonucleases (RFLPs/PCR) revealed the predominance of HPV-16 and HPV-33 in the samples from the State of Alagoas, Brazil.
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spelling High quality DNA from human papillomavirus (HPV) for PCR/RFLPsHuman papillomavirusDNA purificationPCRRFLPThe analysis of DNA in clinical samples for a secure diagnostic has become indispensable nowadays. Techniques approaching isolation of high molecular weigth DNA of HPV could lead to efficient amplification and early clinical diagnosis of the virus DNA by PCR (polymerase chain reaction). We describe a fast, non-toxical, efficient and cheap method for DNA isolation of human papilloma virus (HPV) from cervical smears using guanidine (DNAzol solution). A 450 bp DNA band correponding to the late region (L1) of the virus genome was detected by PCR, showing that the DNAzol extraction soluction generated a good viral DNA yield. The electrophoretic pattern after digestion with restriction endonucleases (RFLPs/PCR) revealed the predominance of HPV-16 and HPV-33 in the samples from the State of Alagoas, Brazil.Instituto de Tecnologia do Paraná - Tecpar2005-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000100006Brazilian Archives of Biology and Technology v.48 n.1 2005reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/S1516-89132005000100006info:eu-repo/semantics/openAccessWanderlei-Silva,DeniseNobre,MarianaGonzaga,Rosa SilvaViana,Luciana SilvaRamalho Neto,Eduardoeng2005-04-15T00:00:00Zoai:scielo:S1516-89132005000100006Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2005-04-15T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false
dc.title.none.fl_str_mv High quality DNA from human papillomavirus (HPV) for PCR/RFLPs
title High quality DNA from human papillomavirus (HPV) for PCR/RFLPs
spellingShingle High quality DNA from human papillomavirus (HPV) for PCR/RFLPs
Wanderlei-Silva,Denise
Human papillomavirus
DNA purification
PCR
RFLP
title_short High quality DNA from human papillomavirus (HPV) for PCR/RFLPs
title_full High quality DNA from human papillomavirus (HPV) for PCR/RFLPs
title_fullStr High quality DNA from human papillomavirus (HPV) for PCR/RFLPs
title_full_unstemmed High quality DNA from human papillomavirus (HPV) for PCR/RFLPs
title_sort High quality DNA from human papillomavirus (HPV) for PCR/RFLPs
author Wanderlei-Silva,Denise
author_facet Wanderlei-Silva,Denise
Nobre,Mariana
Gonzaga,Rosa Silva
Viana,Luciana Silva
Ramalho Neto,Eduardo
author_role author
author2 Nobre,Mariana
Gonzaga,Rosa Silva
Viana,Luciana Silva
Ramalho Neto,Eduardo
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Wanderlei-Silva,Denise
Nobre,Mariana
Gonzaga,Rosa Silva
Viana,Luciana Silva
Ramalho Neto,Eduardo
dc.subject.por.fl_str_mv Human papillomavirus
DNA purification
PCR
RFLP
topic Human papillomavirus
DNA purification
PCR
RFLP
description The analysis of DNA in clinical samples for a secure diagnostic has become indispensable nowadays. Techniques approaching isolation of high molecular weigth DNA of HPV could lead to efficient amplification and early clinical diagnosis of the virus DNA by PCR (polymerase chain reaction). We describe a fast, non-toxical, efficient and cheap method for DNA isolation of human papilloma virus (HPV) from cervical smears using guanidine (DNAzol solution). A 450 bp DNA band correponding to the late region (L1) of the virus genome was detected by PCR, showing that the DNAzol extraction soluction generated a good viral DNA yield. The electrophoretic pattern after digestion with restriction endonucleases (RFLPs/PCR) revealed the predominance of HPV-16 and HPV-33 in the samples from the State of Alagoas, Brazil.
publishDate 2005
dc.date.none.fl_str_mv 2005-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000100006
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000100006
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1516-89132005000100006
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
dc.source.none.fl_str_mv Brazilian Archives of Biology and Technology v.48 n.1 2005
reponame:Brazilian Archives of Biology and Technology
instname:Instituto de Tecnologia do Paraná (Tecpar)
instacron:TECPAR
instname_str Instituto de Tecnologia do Paraná (Tecpar)
instacron_str TECPAR
institution TECPAR
reponame_str Brazilian Archives of Biology and Technology
collection Brazilian Archives of Biology and Technology
repository.name.fl_str_mv Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)
repository.mail.fl_str_mv babt@tecpar.br||babt@tecpar.br
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