Evaluation of MALDI-TOF MS System for the Identification and Differentiation of Burkholderia cepacia Complex Species
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Archives of Biology and Technology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132022000100321 |
Resumo: | Abstract To evaluate the performance of MALDI-TOF MS system as a tool for identification and differentiation of Burkholderia cenocepacia from other species of Burkholderia cepacia complex (Bcc). Fifty-three suggestive colonies were submitted to the MALDI-TOF Microflex LT 4.0® (Bruker Daltonik, Bremen, Germany) system for identification. We compared two protocols of protein extraction: (A) Direct Method and (B) Tube Extraction. In parallel, all isolates were subjected to molecular diagnosis (primers for recA gene) to identify species belonging to Bcc and to differentiate B. cenocepacia in genomovar IIIA or IIIB. MALDI-TOF was able to identify all isolates to the genus level and 94.3% (50/53) to species using both methods. The MALDI-TOF system was able to identify 38 out of the 40 isolates identified as B. cenocepacia by molecular techniques. In conclusion, MALDI-TOF Microflex LT 4.0® is a feasible technique and presents a low cost of reagents; it can be used for the reliable differentiation of B. cenocepacia from other species of Bcc. |
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Brazilian Archives of Biology and Technology |
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Evaluation of MALDI-TOF MS System for the Identification and Differentiation of Burkholderia cepacia Complex SpeciesBurkholderia cepacia complexBurkholderia cenocepaciaMALDI-TOF MSPolymerase Chain ReactionAbstract To evaluate the performance of MALDI-TOF MS system as a tool for identification and differentiation of Burkholderia cenocepacia from other species of Burkholderia cepacia complex (Bcc). Fifty-three suggestive colonies were submitted to the MALDI-TOF Microflex LT 4.0® (Bruker Daltonik, Bremen, Germany) system for identification. We compared two protocols of protein extraction: (A) Direct Method and (B) Tube Extraction. In parallel, all isolates were subjected to molecular diagnosis (primers for recA gene) to identify species belonging to Bcc and to differentiate B. cenocepacia in genomovar IIIA or IIIB. MALDI-TOF was able to identify all isolates to the genus level and 94.3% (50/53) to species using both methods. The MALDI-TOF system was able to identify 38 out of the 40 isolates identified as B. cenocepacia by molecular techniques. In conclusion, MALDI-TOF Microflex LT 4.0® is a feasible technique and presents a low cost of reagents; it can be used for the reliable differentiation of B. cenocepacia from other species of Bcc.Instituto de Tecnologia do Paraná - Tecpar2022-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132022000100321Brazilian Archives of Biology and Technology v.65 2022reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/1678-4324-2022210572info:eu-repo/semantics/openAccessVolpato,Fabiana Caroline ZempulskiHernandez,Mayana KielingLima-Morales,Daiana deWink,Priscila LambMartins,Daniela de SouzaOliveira,Katia Ruschel Pilger deBarth,Afonso Luíseng2022-06-02T00:00:00Zoai:scielo:S1516-89132022000100321Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2022-06-02T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false |
dc.title.none.fl_str_mv |
Evaluation of MALDI-TOF MS System for the Identification and Differentiation of Burkholderia cepacia Complex Species |
title |
Evaluation of MALDI-TOF MS System for the Identification and Differentiation of Burkholderia cepacia Complex Species |
spellingShingle |
Evaluation of MALDI-TOF MS System for the Identification and Differentiation of Burkholderia cepacia Complex Species Volpato,Fabiana Caroline Zempulski Burkholderia cepacia complex Burkholderia cenocepacia MALDI-TOF MS Polymerase Chain Reaction |
title_short |
Evaluation of MALDI-TOF MS System for the Identification and Differentiation of Burkholderia cepacia Complex Species |
title_full |
Evaluation of MALDI-TOF MS System for the Identification and Differentiation of Burkholderia cepacia Complex Species |
title_fullStr |
Evaluation of MALDI-TOF MS System for the Identification and Differentiation of Burkholderia cepacia Complex Species |
title_full_unstemmed |
Evaluation of MALDI-TOF MS System for the Identification and Differentiation of Burkholderia cepacia Complex Species |
title_sort |
Evaluation of MALDI-TOF MS System for the Identification and Differentiation of Burkholderia cepacia Complex Species |
author |
Volpato,Fabiana Caroline Zempulski |
author_facet |
Volpato,Fabiana Caroline Zempulski Hernandez,Mayana Kieling Lima-Morales,Daiana de Wink,Priscila Lamb Martins,Daniela de Souza Oliveira,Katia Ruschel Pilger de Barth,Afonso Luís |
author_role |
author |
author2 |
Hernandez,Mayana Kieling Lima-Morales,Daiana de Wink,Priscila Lamb Martins,Daniela de Souza Oliveira,Katia Ruschel Pilger de Barth,Afonso Luís |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Volpato,Fabiana Caroline Zempulski Hernandez,Mayana Kieling Lima-Morales,Daiana de Wink,Priscila Lamb Martins,Daniela de Souza Oliveira,Katia Ruschel Pilger de Barth,Afonso Luís |
dc.subject.por.fl_str_mv |
Burkholderia cepacia complex Burkholderia cenocepacia MALDI-TOF MS Polymerase Chain Reaction |
topic |
Burkholderia cepacia complex Burkholderia cenocepacia MALDI-TOF MS Polymerase Chain Reaction |
description |
Abstract To evaluate the performance of MALDI-TOF MS system as a tool for identification and differentiation of Burkholderia cenocepacia from other species of Burkholderia cepacia complex (Bcc). Fifty-three suggestive colonies were submitted to the MALDI-TOF Microflex LT 4.0® (Bruker Daltonik, Bremen, Germany) system for identification. We compared two protocols of protein extraction: (A) Direct Method and (B) Tube Extraction. In parallel, all isolates were subjected to molecular diagnosis (primers for recA gene) to identify species belonging to Bcc and to differentiate B. cenocepacia in genomovar IIIA or IIIB. MALDI-TOF was able to identify all isolates to the genus level and 94.3% (50/53) to species using both methods. The MALDI-TOF system was able to identify 38 out of the 40 isolates identified as B. cenocepacia by molecular techniques. In conclusion, MALDI-TOF Microflex LT 4.0® is a feasible technique and presents a low cost of reagents; it can be used for the reliable differentiation of B. cenocepacia from other species of Bcc. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132022000100321 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132022000100321 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/1678-4324-2022210572 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Instituto de Tecnologia do Paraná - Tecpar |
publisher.none.fl_str_mv |
Instituto de Tecnologia do Paraná - Tecpar |
dc.source.none.fl_str_mv |
Brazilian Archives of Biology and Technology v.65 2022 reponame:Brazilian Archives of Biology and Technology instname:Instituto de Tecnologia do Paraná (Tecpar) instacron:TECPAR |
instname_str |
Instituto de Tecnologia do Paraná (Tecpar) |
instacron_str |
TECPAR |
institution |
TECPAR |
reponame_str |
Brazilian Archives of Biology and Technology |
collection |
Brazilian Archives of Biology and Technology |
repository.name.fl_str_mv |
Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar) |
repository.mail.fl_str_mv |
babt@tecpar.br||babt@tecpar.br |
_version_ |
1750318281374302208 |