Seleção de bacillus spp. para produção de esterases e melhoramento de bacillus cereus (c124)
Autor(a) principal: | |
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Data de Publicação: | 1998 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Brazilian Archives of Biology and Technology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131998000100011 |
Resumo: | Forty-four Bacillus spp. strains obtained from sugar cane derivates and residues, six of them isolated in this work, were tested using Tween 80 as substrate (agar-Tween 80 medium), in order to determine their esterase activity through the enzymatic index averages. After statistic analysis, B. cereus (C124) strain, which presented better results, was submitted to genetic improvement by treatment with ultraviolet light (UV). The survival curve pointed out 28" as the time necessary to obtain 30% of survivors. Fifty survivors and the wild strain C124 were compared in relation to their esterase activity as mentioned previously. The wild strain and the mutant C124UV35, which showed enzymatic index average higher than C124, were characterized in polyacrilamide gel electrophoresis (PAGE). Eletrophoretic patterns for total proteins of wild and mutant strain showed different profiles according to number, position and intensity of bands. For esterase, the bands varied only in intensity. |
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Seleção de bacillus spp. para produção de esterases e melhoramento de bacillus cereus (c124)Bacteriagenetic improvementBacillus spp.B. cereusesteraseForty-four Bacillus spp. strains obtained from sugar cane derivates and residues, six of them isolated in this work, were tested using Tween 80 as substrate (agar-Tween 80 medium), in order to determine their esterase activity through the enzymatic index averages. After statistic analysis, B. cereus (C124) strain, which presented better results, was submitted to genetic improvement by treatment with ultraviolet light (UV). The survival curve pointed out 28" as the time necessary to obtain 30% of survivors. Fifty survivors and the wild strain C124 were compared in relation to their esterase activity as mentioned previously. The wild strain and the mutant C124UV35, which showed enzymatic index average higher than C124, were characterized in polyacrilamide gel electrophoresis (PAGE). Eletrophoretic patterns for total proteins of wild and mutant strain showed different profiles according to number, position and intensity of bands. For esterase, the bands varied only in intensity.Instituto de Tecnologia do Paraná - Tecpar1998-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131998000100011Brazilian Archives of Biology and Technology v.41 n.1 1998reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/S1516-89131998000100011info:eu-repo/semantics/openAccessMendonça,Analucia LongmanMariano,Rosa de Lima RamosAraújo,Janete Magali deCavalcante,Uided Maaze T.por2011-09-21T00:00:00Zoai:scielo:S1516-89131998000100011Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2011-09-21T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false |
dc.title.none.fl_str_mv |
Seleção de bacillus spp. para produção de esterases e melhoramento de bacillus cereus (c124) |
title |
Seleção de bacillus spp. para produção de esterases e melhoramento de bacillus cereus (c124) |
spellingShingle |
Seleção de bacillus spp. para produção de esterases e melhoramento de bacillus cereus (c124) Mendonça,Analucia Longman Bacteria genetic improvement Bacillus spp. B. cereus esterase |
title_short |
Seleção de bacillus spp. para produção de esterases e melhoramento de bacillus cereus (c124) |
title_full |
Seleção de bacillus spp. para produção de esterases e melhoramento de bacillus cereus (c124) |
title_fullStr |
Seleção de bacillus spp. para produção de esterases e melhoramento de bacillus cereus (c124) |
title_full_unstemmed |
Seleção de bacillus spp. para produção de esterases e melhoramento de bacillus cereus (c124) |
title_sort |
Seleção de bacillus spp. para produção de esterases e melhoramento de bacillus cereus (c124) |
author |
Mendonça,Analucia Longman |
author_facet |
Mendonça,Analucia Longman Mariano,Rosa de Lima Ramos Araújo,Janete Magali de Cavalcante,Uided Maaze T. |
author_role |
author |
author2 |
Mariano,Rosa de Lima Ramos Araújo,Janete Magali de Cavalcante,Uided Maaze T. |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Mendonça,Analucia Longman Mariano,Rosa de Lima Ramos Araújo,Janete Magali de Cavalcante,Uided Maaze T. |
dc.subject.por.fl_str_mv |
Bacteria genetic improvement Bacillus spp. B. cereus esterase |
topic |
Bacteria genetic improvement Bacillus spp. B. cereus esterase |
description |
Forty-four Bacillus spp. strains obtained from sugar cane derivates and residues, six of them isolated in this work, were tested using Tween 80 as substrate (agar-Tween 80 medium), in order to determine their esterase activity through the enzymatic index averages. After statistic analysis, B. cereus (C124) strain, which presented better results, was submitted to genetic improvement by treatment with ultraviolet light (UV). The survival curve pointed out 28" as the time necessary to obtain 30% of survivors. Fifty survivors and the wild strain C124 were compared in relation to their esterase activity as mentioned previously. The wild strain and the mutant C124UV35, which showed enzymatic index average higher than C124, were characterized in polyacrilamide gel electrophoresis (PAGE). Eletrophoretic patterns for total proteins of wild and mutant strain showed different profiles according to number, position and intensity of bands. For esterase, the bands varied only in intensity. |
publishDate |
1998 |
dc.date.none.fl_str_mv |
1998-06-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131998000100011 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131998000100011 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
10.1590/S1516-89131998000100011 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Instituto de Tecnologia do Paraná - Tecpar |
publisher.none.fl_str_mv |
Instituto de Tecnologia do Paraná - Tecpar |
dc.source.none.fl_str_mv |
Brazilian Archives of Biology and Technology v.41 n.1 1998 reponame:Brazilian Archives of Biology and Technology instname:Instituto de Tecnologia do Paraná (Tecpar) instacron:TECPAR |
instname_str |
Instituto de Tecnologia do Paraná (Tecpar) |
instacron_str |
TECPAR |
institution |
TECPAR |
reponame_str |
Brazilian Archives of Biology and Technology |
collection |
Brazilian Archives of Biology and Technology |
repository.name.fl_str_mv |
Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar) |
repository.mail.fl_str_mv |
babt@tecpar.br||babt@tecpar.br |
_version_ |
1750318267808874496 |