In vitro shoot regeneration from roots and leaf discs of Passiflora cincinnata mast.
Autor(a) principal: | |
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Data de Publicação: | 2007 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Archives of Biology and Technology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000200009 |
Resumo: | Different concentrations of 6-benzyladenine (6-BA) were used in vitro to induce buds in three types of explants: leaf discs, root segments and the seedling itself obtained from in vitro seed germination of Passiflora cincinnata Mast. The 0.5 mg.L -1 6-BA concentration was most suitable for all the three explants, however, bud formation time and means (direct/indirect) were different for each type of explant. In seedlings used as explants, it had shoot regeneration from the primary root. Histological events leading to bud formation via meristemoids were described. The origin was indirect in leaf discs, with callus formation in subepidermal cells of the chlorophyll parenchyma layers. In root segments and in seedling roots, the buds were formed directly in the pericycle (in roots with some secondary structure) and in the vascular cambium (at the initial phase of secondary structure). Also, indirect buds originated from meristemoids which were formed around the callus. |
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In vitro shoot regeneration from roots and leaf discs of Passiflora cincinnata mast.Anatomicalorganogenesis6-benzyladeninepassionfruitDifferent concentrations of 6-benzyladenine (6-BA) were used in vitro to induce buds in three types of explants: leaf discs, root segments and the seedling itself obtained from in vitro seed germination of Passiflora cincinnata Mast. The 0.5 mg.L -1 6-BA concentration was most suitable for all the three explants, however, bud formation time and means (direct/indirect) were different for each type of explant. In seedlings used as explants, it had shoot regeneration from the primary root. Histological events leading to bud formation via meristemoids were described. The origin was indirect in leaf discs, with callus formation in subepidermal cells of the chlorophyll parenchyma layers. In root segments and in seedling roots, the buds were formed directly in the pericycle (in roots with some secondary structure) and in the vascular cambium (at the initial phase of secondary structure). Also, indirect buds originated from meristemoids which were formed around the callus.Instituto de Tecnologia do Paraná - Tecpar2007-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000200009Brazilian Archives of Biology and Technology v.50 n.2 2007reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/S1516-89132007000200009info:eu-repo/semantics/openAccessLombardi,Simone PachecoPassos,Ilene Ribeiro da SilvaNogueira,Maria Cristina StolfAppezzato-da-Glória,Beatrizeng2007-06-12T00:00:00Zoai:scielo:S1516-89132007000200009Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2007-06-12T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false |
dc.title.none.fl_str_mv |
In vitro shoot regeneration from roots and leaf discs of Passiflora cincinnata mast. |
title |
In vitro shoot regeneration from roots and leaf discs of Passiflora cincinnata mast. |
spellingShingle |
In vitro shoot regeneration from roots and leaf discs of Passiflora cincinnata mast. Lombardi,Simone Pacheco Anatomical organogenesis 6-benzyladenine passionfruit |
title_short |
In vitro shoot regeneration from roots and leaf discs of Passiflora cincinnata mast. |
title_full |
In vitro shoot regeneration from roots and leaf discs of Passiflora cincinnata mast. |
title_fullStr |
In vitro shoot regeneration from roots and leaf discs of Passiflora cincinnata mast. |
title_full_unstemmed |
In vitro shoot regeneration from roots and leaf discs of Passiflora cincinnata mast. |
title_sort |
In vitro shoot regeneration from roots and leaf discs of Passiflora cincinnata mast. |
author |
Lombardi,Simone Pacheco |
author_facet |
Lombardi,Simone Pacheco Passos,Ilene Ribeiro da Silva Nogueira,Maria Cristina Stolf Appezzato-da-Glória,Beatriz |
author_role |
author |
author2 |
Passos,Ilene Ribeiro da Silva Nogueira,Maria Cristina Stolf Appezzato-da-Glória,Beatriz |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Lombardi,Simone Pacheco Passos,Ilene Ribeiro da Silva Nogueira,Maria Cristina Stolf Appezzato-da-Glória,Beatriz |
dc.subject.por.fl_str_mv |
Anatomical organogenesis 6-benzyladenine passionfruit |
topic |
Anatomical organogenesis 6-benzyladenine passionfruit |
description |
Different concentrations of 6-benzyladenine (6-BA) were used in vitro to induce buds in three types of explants: leaf discs, root segments and the seedling itself obtained from in vitro seed germination of Passiflora cincinnata Mast. The 0.5 mg.L -1 6-BA concentration was most suitable for all the three explants, however, bud formation time and means (direct/indirect) were different for each type of explant. In seedlings used as explants, it had shoot regeneration from the primary root. Histological events leading to bud formation via meristemoids were described. The origin was indirect in leaf discs, with callus formation in subepidermal cells of the chlorophyll parenchyma layers. In root segments and in seedling roots, the buds were formed directly in the pericycle (in roots with some secondary structure) and in the vascular cambium (at the initial phase of secondary structure). Also, indirect buds originated from meristemoids which were formed around the callus. |
publishDate |
2007 |
dc.date.none.fl_str_mv |
2007-03-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000200009 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000200009 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1516-89132007000200009 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Instituto de Tecnologia do Paraná - Tecpar |
publisher.none.fl_str_mv |
Instituto de Tecnologia do Paraná - Tecpar |
dc.source.none.fl_str_mv |
Brazilian Archives of Biology and Technology v.50 n.2 2007 reponame:Brazilian Archives of Biology and Technology instname:Instituto de Tecnologia do Paraná (Tecpar) instacron:TECPAR |
instname_str |
Instituto de Tecnologia do Paraná (Tecpar) |
instacron_str |
TECPAR |
institution |
TECPAR |
reponame_str |
Brazilian Archives of Biology and Technology |
collection |
Brazilian Archives of Biology and Technology |
repository.name.fl_str_mv |
Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar) |
repository.mail.fl_str_mv |
babt@tecpar.br||babt@tecpar.br |
_version_ |
1750318271480987648 |