Damage assessment of the equine sperm membranes by fluorimetric technique
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Archives of Biology and Technology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000600004 |
Resumo: | To validate a practical technique of simultaneous evaluation of the plasma, acrosomal and mitochondrial membranes in equine spermatozoa three fluorescent probes (PI, FITC-PSA and MITO) were associated. Four ejaculates from three stallions (n=12) were diluted in TALP medium and split into 2 aliquots, 1 aliquot was flash frozen in liquid nitrogen to induce damage in cellular membranes. Three treatments were prepared with the following fixed ratios of fresh semen: flash frozen semen: 100:0 (T100), 50:50 (T50), and 0:100 (T0). A 150-µL aliquot of diluted semen of each treatment was added of 2 µL of PI, 2 µL of MITO and 80 µL of FITC-PSA; incubated at 38.5ºC/8 min, and sperm cells were evaluated by epifluorescent microscopy. Based in regression analysis, this could be an efficient and practical technique to assess damage in equine spermatozoa, as it was able to determine the sperm percentage more representative of the potential to fertilize the oocyte. |
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Brazilian Archives of Biology and Technology |
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Damage assessment of the equine sperm membranes by fluorimetric techniqueStallionacrosomeplasma membranemitochondriafluorescent probesTo validate a practical technique of simultaneous evaluation of the plasma, acrosomal and mitochondrial membranes in equine spermatozoa three fluorescent probes (PI, FITC-PSA and MITO) were associated. Four ejaculates from three stallions (n=12) were diluted in TALP medium and split into 2 aliquots, 1 aliquot was flash frozen in liquid nitrogen to induce damage in cellular membranes. Three treatments were prepared with the following fixed ratios of fresh semen: flash frozen semen: 100:0 (T100), 50:50 (T50), and 0:100 (T0). A 150-µL aliquot of diluted semen of each treatment was added of 2 µL of PI, 2 µL of MITO and 80 µL of FITC-PSA; incubated at 38.5ºC/8 min, and sperm cells were evaluated by epifluorescent microscopy. Based in regression analysis, this could be an efficient and practical technique to assess damage in equine spermatozoa, as it was able to determine the sperm percentage more representative of the potential to fertilize the oocyte.Instituto de Tecnologia do Paraná - Tecpar2010-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000600004Brazilian Archives of Biology and Technology v.53 n.6 2010reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/S1516-89132010000600004info:eu-repo/semantics/openAccessCeleghini,Eneiva Carla CarvalhoAndrade,André Furugen Cesar deRaphael,Cláudia FernandesNascimento,JulianaTicianelli,Janahi SouzaArruda,Rubens Paes deeng2011-01-18T00:00:00Zoai:scielo:S1516-89132010000600004Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2011-01-18T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false |
dc.title.none.fl_str_mv |
Damage assessment of the equine sperm membranes by fluorimetric technique |
title |
Damage assessment of the equine sperm membranes by fluorimetric technique |
spellingShingle |
Damage assessment of the equine sperm membranes by fluorimetric technique Celeghini,Eneiva Carla Carvalho Stallion acrosome plasma membrane mitochondria fluorescent probes |
title_short |
Damage assessment of the equine sperm membranes by fluorimetric technique |
title_full |
Damage assessment of the equine sperm membranes by fluorimetric technique |
title_fullStr |
Damage assessment of the equine sperm membranes by fluorimetric technique |
title_full_unstemmed |
Damage assessment of the equine sperm membranes by fluorimetric technique |
title_sort |
Damage assessment of the equine sperm membranes by fluorimetric technique |
author |
Celeghini,Eneiva Carla Carvalho |
author_facet |
Celeghini,Eneiva Carla Carvalho Andrade,André Furugen Cesar de Raphael,Cláudia Fernandes Nascimento,Juliana Ticianelli,Janahi Souza Arruda,Rubens Paes de |
author_role |
author |
author2 |
Andrade,André Furugen Cesar de Raphael,Cláudia Fernandes Nascimento,Juliana Ticianelli,Janahi Souza Arruda,Rubens Paes de |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Celeghini,Eneiva Carla Carvalho Andrade,André Furugen Cesar de Raphael,Cláudia Fernandes Nascimento,Juliana Ticianelli,Janahi Souza Arruda,Rubens Paes de |
dc.subject.por.fl_str_mv |
Stallion acrosome plasma membrane mitochondria fluorescent probes |
topic |
Stallion acrosome plasma membrane mitochondria fluorescent probes |
description |
To validate a practical technique of simultaneous evaluation of the plasma, acrosomal and mitochondrial membranes in equine spermatozoa three fluorescent probes (PI, FITC-PSA and MITO) were associated. Four ejaculates from three stallions (n=12) were diluted in TALP medium and split into 2 aliquots, 1 aliquot was flash frozen in liquid nitrogen to induce damage in cellular membranes. Three treatments were prepared with the following fixed ratios of fresh semen: flash frozen semen: 100:0 (T100), 50:50 (T50), and 0:100 (T0). A 150-µL aliquot of diluted semen of each treatment was added of 2 µL of PI, 2 µL of MITO and 80 µL of FITC-PSA; incubated at 38.5ºC/8 min, and sperm cells were evaluated by epifluorescent microscopy. Based in regression analysis, this could be an efficient and practical technique to assess damage in equine spermatozoa, as it was able to determine the sperm percentage more representative of the potential to fertilize the oocyte. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010-12-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000600004 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000600004 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1516-89132010000600004 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Instituto de Tecnologia do Paraná - Tecpar |
publisher.none.fl_str_mv |
Instituto de Tecnologia do Paraná - Tecpar |
dc.source.none.fl_str_mv |
Brazilian Archives of Biology and Technology v.53 n.6 2010 reponame:Brazilian Archives of Biology and Technology instname:Instituto de Tecnologia do Paraná (Tecpar) instacron:TECPAR |
instname_str |
Instituto de Tecnologia do Paraná (Tecpar) |
instacron_str |
TECPAR |
institution |
TECPAR |
reponame_str |
Brazilian Archives of Biology and Technology |
collection |
Brazilian Archives of Biology and Technology |
repository.name.fl_str_mv |
Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar) |
repository.mail.fl_str_mv |
babt@tecpar.br||babt@tecpar.br |
_version_ |
1750318274335211520 |