Somatic embryogenesis and plant regeneration from callus cultures of Cleome rosea Vahl

Detalhes bibliográficos
Autor(a) principal: Simões,Claudia
Data de Publicação: 2010
Outros Autores: Albarello,Norma, Callado,Cátia Henriques, Castro,Tatiana Carvalho de, Mansur,Elisabeth
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Archives of Biology and Technology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000300024
Resumo: This paper describes a protocol for the efficient vegetative propagation of Cleome rosea by somatic embryogenesis. Leaf and stem explants from nursery-grown seedlings of C. rosea were cultivated on Murashige and Skoog (MS) medium supplemented with indole-3-acetic acid (IAA), a -naphthaleneacetic acid (NAA), 4-amino-3,5,6-trichloropicolinic acid (picloram) or 2,4-dichlorophenoxyacetic acid (2,4-D). Nodular calli were produced from both explant types in the presence of 4.5 and 9.0 µM 2,4-D. Embryo development and maturation were achieved when calli from stem explants were transferred to media containing a ten-fold reduction of 2,4-D concentration initially used (0.45 and 0.90 µM). Leaf-derived calli did not form embryos with the same treatments. The highest frequency of embryogenic callus formation (85%) and number of embryo per callus (13.45 ± 2.8) were achieved during the first subculture on medium supplemented with 0.90 µM 2,4-D. Embryo conversion into plantlets was achieved following transfer to growth regulator-free MS medium solidified with 2 g.L-1 phytagel. An acclimatization rate of 53% was found three months after transfer to ex vitro conditions and the recovered plants presented a normal phenotypic aspect.
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spelling Somatic embryogenesis and plant regeneration from callus cultures of Cleome rosea Vahlauxinembriogenic callushistologyphytagelsomatic embryosThis paper describes a protocol for the efficient vegetative propagation of Cleome rosea by somatic embryogenesis. Leaf and stem explants from nursery-grown seedlings of C. rosea were cultivated on Murashige and Skoog (MS) medium supplemented with indole-3-acetic acid (IAA), a -naphthaleneacetic acid (NAA), 4-amino-3,5,6-trichloropicolinic acid (picloram) or 2,4-dichlorophenoxyacetic acid (2,4-D). Nodular calli were produced from both explant types in the presence of 4.5 and 9.0 µM 2,4-D. Embryo development and maturation were achieved when calli from stem explants were transferred to media containing a ten-fold reduction of 2,4-D concentration initially used (0.45 and 0.90 µM). Leaf-derived calli did not form embryos with the same treatments. The highest frequency of embryogenic callus formation (85%) and number of embryo per callus (13.45 ± 2.8) were achieved during the first subculture on medium supplemented with 0.90 µM 2,4-D. Embryo conversion into plantlets was achieved following transfer to growth regulator-free MS medium solidified with 2 g.L-1 phytagel. An acclimatization rate of 53% was found three months after transfer to ex vitro conditions and the recovered plants presented a normal phenotypic aspect.Instituto de Tecnologia do Paraná - Tecpar2010-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000300024Brazilian Archives of Biology and Technology v.53 n.3 2010reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/S1516-89132010000300024info:eu-repo/semantics/openAccessSimões,ClaudiaAlbarello,NormaCallado,Cátia HenriquesCastro,Tatiana Carvalho deMansur,Elisabetheng2010-08-12T00:00:00Zoai:scielo:S1516-89132010000300024Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2010-08-12T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false
dc.title.none.fl_str_mv Somatic embryogenesis and plant regeneration from callus cultures of Cleome rosea Vahl
title Somatic embryogenesis and plant regeneration from callus cultures of Cleome rosea Vahl
spellingShingle Somatic embryogenesis and plant regeneration from callus cultures of Cleome rosea Vahl
Simões,Claudia
auxin
embriogenic callus
histology
phytagel
somatic embryos
title_short Somatic embryogenesis and plant regeneration from callus cultures of Cleome rosea Vahl
title_full Somatic embryogenesis and plant regeneration from callus cultures of Cleome rosea Vahl
title_fullStr Somatic embryogenesis and plant regeneration from callus cultures of Cleome rosea Vahl
title_full_unstemmed Somatic embryogenesis and plant regeneration from callus cultures of Cleome rosea Vahl
title_sort Somatic embryogenesis and plant regeneration from callus cultures of Cleome rosea Vahl
author Simões,Claudia
author_facet Simões,Claudia
Albarello,Norma
Callado,Cátia Henriques
Castro,Tatiana Carvalho de
Mansur,Elisabeth
author_role author
author2 Albarello,Norma
Callado,Cátia Henriques
Castro,Tatiana Carvalho de
Mansur,Elisabeth
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Simões,Claudia
Albarello,Norma
Callado,Cátia Henriques
Castro,Tatiana Carvalho de
Mansur,Elisabeth
dc.subject.por.fl_str_mv auxin
embriogenic callus
histology
phytagel
somatic embryos
topic auxin
embriogenic callus
histology
phytagel
somatic embryos
description This paper describes a protocol for the efficient vegetative propagation of Cleome rosea by somatic embryogenesis. Leaf and stem explants from nursery-grown seedlings of C. rosea were cultivated on Murashige and Skoog (MS) medium supplemented with indole-3-acetic acid (IAA), a -naphthaleneacetic acid (NAA), 4-amino-3,5,6-trichloropicolinic acid (picloram) or 2,4-dichlorophenoxyacetic acid (2,4-D). Nodular calli were produced from both explant types in the presence of 4.5 and 9.0 µM 2,4-D. Embryo development and maturation were achieved when calli from stem explants were transferred to media containing a ten-fold reduction of 2,4-D concentration initially used (0.45 and 0.90 µM). Leaf-derived calli did not form embryos with the same treatments. The highest frequency of embryogenic callus formation (85%) and number of embryo per callus (13.45 ± 2.8) were achieved during the first subculture on medium supplemented with 0.90 µM 2,4-D. Embryo conversion into plantlets was achieved following transfer to growth regulator-free MS medium solidified with 2 g.L-1 phytagel. An acclimatization rate of 53% was found three months after transfer to ex vitro conditions and the recovered plants presented a normal phenotypic aspect.
publishDate 2010
dc.date.none.fl_str_mv 2010-06-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000300024
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000300024
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1516-89132010000300024
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
dc.source.none.fl_str_mv Brazilian Archives of Biology and Technology v.53 n.3 2010
reponame:Brazilian Archives of Biology and Technology
instname:Instituto de Tecnologia do Paraná (Tecpar)
instacron:TECPAR
instname_str Instituto de Tecnologia do Paraná (Tecpar)
instacron_str TECPAR
institution TECPAR
reponame_str Brazilian Archives of Biology and Technology
collection Brazilian Archives of Biology and Technology
repository.name.fl_str_mv Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)
repository.mail.fl_str_mv babt@tecpar.br||babt@tecpar.br
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