Bioaccumulation of silver and the isolation of metal-binding protein from P.diminuta
Autor(a) principal: | |
---|---|
Data de Publicação: | 2001 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Archives of Biology and Technology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132001000300001 |
Resumo: | A silver uptake study by Pseudomonas diminuta was carried out by growing the bacteria in a chloride-free medium (CFM) containing silver ions (50 muM) in a batch culture. From the results, it was found that higher amounts of silver were accumulated inside the cell during early exponential phase compared to the amount bound at the cell surface. This suggested a possible mechanism for metal uptake during bacterial growth. In view of this, attempts were made to isolate proteins which might be associated with silver-binding properties from cultures of P.diminuta grown in the presence and absence of silver. The proteins were first extracted from the bacterial cultures by precipitation with ammonium sulfate followed by purification using isoelectric focussing and SDS-PAGE. Results of the experiment showed the presence of low molecular weight and high molecular weight proteins containing silver with pI values ranging from 2.0 to 9.0 for bacteria grown in the presence of silver. |
id |
TECPAR-1_5983c579b6391ccc801e4d7d72090390 |
---|---|
oai_identifier_str |
oai:scielo:S1516-89132001000300001 |
network_acronym_str |
TECPAR-1 |
network_name_str |
Brazilian Archives of Biology and Technology |
repository_id_str |
|
spelling |
Bioaccumulation of silver and the isolation of metal-binding protein from P.diminutaBioaccumulationmetal-binding proteinsPseudomonas diminutaA silver uptake study by Pseudomonas diminuta was carried out by growing the bacteria in a chloride-free medium (CFM) containing silver ions (50 muM) in a batch culture. From the results, it was found that higher amounts of silver were accumulated inside the cell during early exponential phase compared to the amount bound at the cell surface. This suggested a possible mechanism for metal uptake during bacterial growth. In view of this, attempts were made to isolate proteins which might be associated with silver-binding properties from cultures of P.diminuta grown in the presence and absence of silver. The proteins were first extracted from the bacterial cultures by precipitation with ammonium sulfate followed by purification using isoelectric focussing and SDS-PAGE. Results of the experiment showed the presence of low molecular weight and high molecular weight proteins containing silver with pI values ranging from 2.0 to 9.0 for bacteria grown in the presence of silver.Instituto de Tecnologia do Paraná - Tecpar2001-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132001000300001Brazilian Archives of Biology and Technology v.44 n.3 2001reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/S1516-89132001000300001info:eu-repo/semantics/openAccessIbrahim,ZaharahAhmad,Wan AzlinaBaba,Abu Bakareng2002-01-23T00:00:00Zoai:scielo:S1516-89132001000300001Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2002-01-23T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false |
dc.title.none.fl_str_mv |
Bioaccumulation of silver and the isolation of metal-binding protein from P.diminuta |
title |
Bioaccumulation of silver and the isolation of metal-binding protein from P.diminuta |
spellingShingle |
Bioaccumulation of silver and the isolation of metal-binding protein from P.diminuta Ibrahim,Zaharah Bioaccumulation metal-binding proteins Pseudomonas diminuta |
title_short |
Bioaccumulation of silver and the isolation of metal-binding protein from P.diminuta |
title_full |
Bioaccumulation of silver and the isolation of metal-binding protein from P.diminuta |
title_fullStr |
Bioaccumulation of silver and the isolation of metal-binding protein from P.diminuta |
title_full_unstemmed |
Bioaccumulation of silver and the isolation of metal-binding protein from P.diminuta |
title_sort |
Bioaccumulation of silver and the isolation of metal-binding protein from P.diminuta |
author |
Ibrahim,Zaharah |
author_facet |
Ibrahim,Zaharah Ahmad,Wan Azlina Baba,Abu Bakar |
author_role |
author |
author2 |
Ahmad,Wan Azlina Baba,Abu Bakar |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Ibrahim,Zaharah Ahmad,Wan Azlina Baba,Abu Bakar |
dc.subject.por.fl_str_mv |
Bioaccumulation metal-binding proteins Pseudomonas diminuta |
topic |
Bioaccumulation metal-binding proteins Pseudomonas diminuta |
description |
A silver uptake study by Pseudomonas diminuta was carried out by growing the bacteria in a chloride-free medium (CFM) containing silver ions (50 muM) in a batch culture. From the results, it was found that higher amounts of silver were accumulated inside the cell during early exponential phase compared to the amount bound at the cell surface. This suggested a possible mechanism for metal uptake during bacterial growth. In view of this, attempts were made to isolate proteins which might be associated with silver-binding properties from cultures of P.diminuta grown in the presence and absence of silver. The proteins were first extracted from the bacterial cultures by precipitation with ammonium sulfate followed by purification using isoelectric focussing and SDS-PAGE. Results of the experiment showed the presence of low molecular weight and high molecular weight proteins containing silver with pI values ranging from 2.0 to 9.0 for bacteria grown in the presence of silver. |
publishDate |
2001 |
dc.date.none.fl_str_mv |
2001-09-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132001000300001 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132001000300001 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1516-89132001000300001 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Instituto de Tecnologia do Paraná - Tecpar |
publisher.none.fl_str_mv |
Instituto de Tecnologia do Paraná - Tecpar |
dc.source.none.fl_str_mv |
Brazilian Archives of Biology and Technology v.44 n.3 2001 reponame:Brazilian Archives of Biology and Technology instname:Instituto de Tecnologia do Paraná (Tecpar) instacron:TECPAR |
instname_str |
Instituto de Tecnologia do Paraná (Tecpar) |
instacron_str |
TECPAR |
institution |
TECPAR |
reponame_str |
Brazilian Archives of Biology and Technology |
collection |
Brazilian Archives of Biology and Technology |
repository.name.fl_str_mv |
Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar) |
repository.mail.fl_str_mv |
babt@tecpar.br||babt@tecpar.br |
_version_ |
1750318268671852544 |