Improved method for isolation of coupled mitochondria of Araucaria angustifolia (Bert.) O. Kuntze

Detalhes bibliográficos
Autor(a) principal: Mariano,André Bellin
Data de Publicação: 2004
Outros Autores: Kovalhuk,Leonardo, Valente,Caroline, Maurer-Menestrina,Juliana, Pereira-Netto,Adaucto Bellarmino, Guerra,Miguel Pedro, Carnieri,Eva Gunilla Skare
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Archives of Biology and Technology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000600006
Resumo: A method for the isolation of coupled mitochondria from the callus of Araucaria angustifolia is described for the first time. Mitochondria were isolated from embryogenic callus of A. angustifolia. They were metabolically active, able to sustain oxidative phosphorylation as shown by respiratory control ratio values, which were about 2.4 when respiring on succinate as substrate. Oxygen uptake experiments, using freeze-thawed disrupted mitochondria, showed the presence of alternative rotenone-insensitive NAD(P)H dehydrogenases, which were stimulated by Ca2+. The procedure now described for the isolation of A. angustifolia mitochondria is an important new tool, allowing the investigation of mitochondrial bioenergetics and metabolism and physiology of plants.
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spelling Improved method for isolation of coupled mitochondria of Araucaria angustifolia (Bert.) O. KuntzeAraucaria angustifoliacallusplant mitochondriarespiratory chainA method for the isolation of coupled mitochondria from the callus of Araucaria angustifolia is described for the first time. Mitochondria were isolated from embryogenic callus of A. angustifolia. They were metabolically active, able to sustain oxidative phosphorylation as shown by respiratory control ratio values, which were about 2.4 when respiring on succinate as substrate. Oxygen uptake experiments, using freeze-thawed disrupted mitochondria, showed the presence of alternative rotenone-insensitive NAD(P)H dehydrogenases, which were stimulated by Ca2+. The procedure now described for the isolation of A. angustifolia mitochondria is an important new tool, allowing the investigation of mitochondrial bioenergetics and metabolism and physiology of plants.Instituto de Tecnologia do Paraná - Tecpar2004-11-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000600006Brazilian Archives of Biology and Technology v.47 n.6 2004reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/S1516-89132004000600006info:eu-repo/semantics/openAccessMariano,André BellinKovalhuk,LeonardoValente,CarolineMaurer-Menestrina,JulianaPereira-Netto,Adaucto BellarminoGuerra,Miguel PedroCarnieri,Eva Gunilla Skareeng2005-02-04T00:00:00Zoai:scielo:S1516-89132004000600006Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2005-02-04T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false
dc.title.none.fl_str_mv Improved method for isolation of coupled mitochondria of Araucaria angustifolia (Bert.) O. Kuntze
title Improved method for isolation of coupled mitochondria of Araucaria angustifolia (Bert.) O. Kuntze
spellingShingle Improved method for isolation of coupled mitochondria of Araucaria angustifolia (Bert.) O. Kuntze
Mariano,André Bellin
Araucaria angustifolia
callus
plant mitochondria
respiratory chain
title_short Improved method for isolation of coupled mitochondria of Araucaria angustifolia (Bert.) O. Kuntze
title_full Improved method for isolation of coupled mitochondria of Araucaria angustifolia (Bert.) O. Kuntze
title_fullStr Improved method for isolation of coupled mitochondria of Araucaria angustifolia (Bert.) O. Kuntze
title_full_unstemmed Improved method for isolation of coupled mitochondria of Araucaria angustifolia (Bert.) O. Kuntze
title_sort Improved method for isolation of coupled mitochondria of Araucaria angustifolia (Bert.) O. Kuntze
author Mariano,André Bellin
author_facet Mariano,André Bellin
Kovalhuk,Leonardo
Valente,Caroline
Maurer-Menestrina,Juliana
Pereira-Netto,Adaucto Bellarmino
Guerra,Miguel Pedro
Carnieri,Eva Gunilla Skare
author_role author
author2 Kovalhuk,Leonardo
Valente,Caroline
Maurer-Menestrina,Juliana
Pereira-Netto,Adaucto Bellarmino
Guerra,Miguel Pedro
Carnieri,Eva Gunilla Skare
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Mariano,André Bellin
Kovalhuk,Leonardo
Valente,Caroline
Maurer-Menestrina,Juliana
Pereira-Netto,Adaucto Bellarmino
Guerra,Miguel Pedro
Carnieri,Eva Gunilla Skare
dc.subject.por.fl_str_mv Araucaria angustifolia
callus
plant mitochondria
respiratory chain
topic Araucaria angustifolia
callus
plant mitochondria
respiratory chain
description A method for the isolation of coupled mitochondria from the callus of Araucaria angustifolia is described for the first time. Mitochondria were isolated from embryogenic callus of A. angustifolia. They were metabolically active, able to sustain oxidative phosphorylation as shown by respiratory control ratio values, which were about 2.4 when respiring on succinate as substrate. Oxygen uptake experiments, using freeze-thawed disrupted mitochondria, showed the presence of alternative rotenone-insensitive NAD(P)H dehydrogenases, which were stimulated by Ca2+. The procedure now described for the isolation of A. angustifolia mitochondria is an important new tool, allowing the investigation of mitochondrial bioenergetics and metabolism and physiology of plants.
publishDate 2004
dc.date.none.fl_str_mv 2004-11-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000600006
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000600006
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1516-89132004000600006
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
dc.source.none.fl_str_mv Brazilian Archives of Biology and Technology v.47 n.6 2004
reponame:Brazilian Archives of Biology and Technology
instname:Instituto de Tecnologia do Paraná (Tecpar)
instacron:TECPAR
instname_str Instituto de Tecnologia do Paraná (Tecpar)
instacron_str TECPAR
institution TECPAR
reponame_str Brazilian Archives of Biology and Technology
collection Brazilian Archives of Biology and Technology
repository.name.fl_str_mv Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)
repository.mail.fl_str_mv babt@tecpar.br||babt@tecpar.br
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