Improved method for isolation of coupled mitochondria of Araucaria angustifolia (Bert.) O. Kuntze
Autor(a) principal: | |
---|---|
Data de Publicação: | 2004 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Archives of Biology and Technology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000600006 |
Resumo: | A method for the isolation of coupled mitochondria from the callus of Araucaria angustifolia is described for the first time. Mitochondria were isolated from embryogenic callus of A. angustifolia. They were metabolically active, able to sustain oxidative phosphorylation as shown by respiratory control ratio values, which were about 2.4 when respiring on succinate as substrate. Oxygen uptake experiments, using freeze-thawed disrupted mitochondria, showed the presence of alternative rotenone-insensitive NAD(P)H dehydrogenases, which were stimulated by Ca2+. The procedure now described for the isolation of A. angustifolia mitochondria is an important new tool, allowing the investigation of mitochondrial bioenergetics and metabolism and physiology of plants. |
id |
TECPAR-1_6861b4049710eb864d52c4058cd45904 |
---|---|
oai_identifier_str |
oai:scielo:S1516-89132004000600006 |
network_acronym_str |
TECPAR-1 |
network_name_str |
Brazilian Archives of Biology and Technology |
repository_id_str |
|
spelling |
Improved method for isolation of coupled mitochondria of Araucaria angustifolia (Bert.) O. KuntzeAraucaria angustifoliacallusplant mitochondriarespiratory chainA method for the isolation of coupled mitochondria from the callus of Araucaria angustifolia is described for the first time. Mitochondria were isolated from embryogenic callus of A. angustifolia. They were metabolically active, able to sustain oxidative phosphorylation as shown by respiratory control ratio values, which were about 2.4 when respiring on succinate as substrate. Oxygen uptake experiments, using freeze-thawed disrupted mitochondria, showed the presence of alternative rotenone-insensitive NAD(P)H dehydrogenases, which were stimulated by Ca2+. The procedure now described for the isolation of A. angustifolia mitochondria is an important new tool, allowing the investigation of mitochondrial bioenergetics and metabolism and physiology of plants.Instituto de Tecnologia do Paraná - Tecpar2004-11-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000600006Brazilian Archives of Biology and Technology v.47 n.6 2004reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/S1516-89132004000600006info:eu-repo/semantics/openAccessMariano,André BellinKovalhuk,LeonardoValente,CarolineMaurer-Menestrina,JulianaPereira-Netto,Adaucto BellarminoGuerra,Miguel PedroCarnieri,Eva Gunilla Skareeng2005-02-04T00:00:00Zoai:scielo:S1516-89132004000600006Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2005-02-04T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false |
dc.title.none.fl_str_mv |
Improved method for isolation of coupled mitochondria of Araucaria angustifolia (Bert.) O. Kuntze |
title |
Improved method for isolation of coupled mitochondria of Araucaria angustifolia (Bert.) O. Kuntze |
spellingShingle |
Improved method for isolation of coupled mitochondria of Araucaria angustifolia (Bert.) O. Kuntze Mariano,André Bellin Araucaria angustifolia callus plant mitochondria respiratory chain |
title_short |
Improved method for isolation of coupled mitochondria of Araucaria angustifolia (Bert.) O. Kuntze |
title_full |
Improved method for isolation of coupled mitochondria of Araucaria angustifolia (Bert.) O. Kuntze |
title_fullStr |
Improved method for isolation of coupled mitochondria of Araucaria angustifolia (Bert.) O. Kuntze |
title_full_unstemmed |
Improved method for isolation of coupled mitochondria of Araucaria angustifolia (Bert.) O. Kuntze |
title_sort |
Improved method for isolation of coupled mitochondria of Araucaria angustifolia (Bert.) O. Kuntze |
author |
Mariano,André Bellin |
author_facet |
Mariano,André Bellin Kovalhuk,Leonardo Valente,Caroline Maurer-Menestrina,Juliana Pereira-Netto,Adaucto Bellarmino Guerra,Miguel Pedro Carnieri,Eva Gunilla Skare |
author_role |
author |
author2 |
Kovalhuk,Leonardo Valente,Caroline Maurer-Menestrina,Juliana Pereira-Netto,Adaucto Bellarmino Guerra,Miguel Pedro Carnieri,Eva Gunilla Skare |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Mariano,André Bellin Kovalhuk,Leonardo Valente,Caroline Maurer-Menestrina,Juliana Pereira-Netto,Adaucto Bellarmino Guerra,Miguel Pedro Carnieri,Eva Gunilla Skare |
dc.subject.por.fl_str_mv |
Araucaria angustifolia callus plant mitochondria respiratory chain |
topic |
Araucaria angustifolia callus plant mitochondria respiratory chain |
description |
A method for the isolation of coupled mitochondria from the callus of Araucaria angustifolia is described for the first time. Mitochondria were isolated from embryogenic callus of A. angustifolia. They were metabolically active, able to sustain oxidative phosphorylation as shown by respiratory control ratio values, which were about 2.4 when respiring on succinate as substrate. Oxygen uptake experiments, using freeze-thawed disrupted mitochondria, showed the presence of alternative rotenone-insensitive NAD(P)H dehydrogenases, which were stimulated by Ca2+. The procedure now described for the isolation of A. angustifolia mitochondria is an important new tool, allowing the investigation of mitochondrial bioenergetics and metabolism and physiology of plants. |
publishDate |
2004 |
dc.date.none.fl_str_mv |
2004-11-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000600006 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000600006 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1516-89132004000600006 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Instituto de Tecnologia do Paraná - Tecpar |
publisher.none.fl_str_mv |
Instituto de Tecnologia do Paraná - Tecpar |
dc.source.none.fl_str_mv |
Brazilian Archives of Biology and Technology v.47 n.6 2004 reponame:Brazilian Archives of Biology and Technology instname:Instituto de Tecnologia do Paraná (Tecpar) instacron:TECPAR |
instname_str |
Instituto de Tecnologia do Paraná (Tecpar) |
instacron_str |
TECPAR |
institution |
TECPAR |
reponame_str |
Brazilian Archives of Biology and Technology |
collection |
Brazilian Archives of Biology and Technology |
repository.name.fl_str_mv |
Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar) |
repository.mail.fl_str_mv |
babt@tecpar.br||babt@tecpar.br |
_version_ |
1750318269929095168 |